FastQCFastQC Report
Sat 18 Jun 2016
SRR1930944_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930944_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3582421
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75100.20963476933615563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59980.16742867463092698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44020.12287779688651891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.052.7835541
GTATCAA69150.042.6384121
ATCAACG88750.032.413133
TCAACGC89200.032.1959464
CAACGCA89750.031.8315325
TATCAAC92900.031.1189122
AACGCAG95900.029.789786
ACGCAGA110600.025.7874727
CGCAGAG113800.025.0206388
GTACATG92300.023.3022631
TACATGG95700.021.726262
ACATGGG95500.021.0755633
GCAGAGT134350.020.6999
AGAGTAC121750.019.91779710-11
CATGGGG68500.019.1955184
GAGTACA72700.018.8087391
GTGGTAT23250.018.5831811
CAGAGTA126050.017.93946510-11
GAGTACT87200.017.74150812-13
TATACCG3251.0153599E-817.522395
Produced by FastQC (version 0.11.5)