##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930944_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3582421 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24361988722152 31.0 31.0 33.0 30.0 34.0 2 31.35334791751165 31.0 31.0 34.0 30.0 34.0 3 31.43654416943179 31.0 31.0 34.0 28.0 34.0 4 35.30237289252157 37.0 35.0 37.0 33.0 37.0 5 35.08835477460634 35.0 35.0 37.0 32.0 37.0 6 35.130966460949175 37.0 35.0 37.0 32.0 37.0 7 35.01386325057831 36.0 35.0 37.0 32.0 37.0 8 34.99445375068983 36.0 35.0 37.0 32.0 37.0 9 36.50084928599961 38.0 35.0 39.0 32.0 39.0 10-11 36.43095870083388 38.0 35.0 39.0 32.0 39.0 12-13 36.32561039029193 38.0 35.0 39.0 32.0 39.0 14-15 37.33313826041105 39.0 36.0 41.0 32.0 41.0 16-17 37.29830734578655 39.0 36.0 40.5 32.0 41.0 18-19 37.28846246714163 39.0 36.0 40.0 32.0 41.0 20-21 37.25239593559775 39.0 36.0 40.0 31.0 41.0 22-23 37.123633570705394 39.0 36.0 40.0 31.0 41.0 24-25 36.999920444861175 39.0 36.0 40.0 31.0 41.0 26-27 36.72043417007661 39.0 35.5 40.0 30.0 41.0 28-29 36.5314207905771 38.0 35.0 40.0 30.0 41.0 30-31 36.29336390111604 38.0 35.0 40.0 30.0 41.0 32-33 36.05854364967155 38.0 34.5 40.0 29.5 41.0 34-35 36.26236880031688 38.0 35.0 40.0 30.0 41.0 36-37 36.311588308576795 38.0 35.0 40.0 30.0 41.0 38-39 36.277188108265335 38.0 35.0 40.0 30.0 41.0 40-41 36.179589305667875 38.0 35.0 40.0 30.0 41.0 42-43 36.04434305739052 38.0 35.0 40.0 29.5 41.0 44-45 35.860857643476294 38.0 35.0 40.0 29.0 41.0 46-47 35.64096165693535 38.0 34.0 40.0 28.0 41.0 48-49 35.43832704196408 38.0 34.0 40.0 27.0 41.0 50-51 35.17300465244035 38.0 34.0 40.0 26.5 41.0 52-53 34.963629484083526 38.0 33.5 40.0 26.0 41.0 54-55 34.787372422169256 38.0 33.0 40.0 26.0 41.0 56-57 34.53204941574427 37.5 33.0 40.0 26.0 41.0 58-59 34.28262423651492 37.0 33.0 40.0 25.5 41.0 60-61 33.96873329516548 37.0 32.5 40.0 25.0 41.0 62-63 33.66068449241449 36.0 32.0 39.0 24.0 41.0 64-65 33.3477673338784 36.0 32.0 39.0 23.0 40.5 66-67 33.008417212828974 35.5 31.0 39.0 23.0 40.0 68-69 32.62831839138951 35.0 31.0 38.5 22.0 40.0 70-71 32.21697310282627 35.0 31.0 38.0 22.0 40.0 72-73 31.790666563198464 35.0 30.5 37.0 20.5 39.5 74-75 31.2640446223378 34.0 30.0 37.0 20.0 39.0 76-77 29.907750791992342 32.5 28.5 35.5 18.0 38.0 78-79 30.670669918471337 34.0 29.5 36.0 19.5 38.0 80-81 30.66419008262848 34.0 30.0 35.5 19.0 37.5 82-83 30.381874855021227 34.0 30.0 35.0 18.0 37.0 84-85 30.051589134833677 34.0 29.5 35.0 17.5 36.5 86-87 29.69634403661658 34.0 29.0 35.0 14.0 36.0 88-89 29.340790208632654 34.0 29.0 35.0 8.5 36.0 90-91 28.985865424527155 34.0 29.0 35.0 6.0 35.5 92-93 28.614000001674846 33.0 29.0 35.0 2.0 35.0 94-95 28.179585118555302 33.0 28.5 35.0 2.0 35.0 96-97 27.69905212145641 33.0 27.0 35.0 2.0 35.0 98-99 27.183872442686106 33.0 27.0 35.0 2.0 35.0 100-101 25.936307876712426 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5175747134921664 1101 2 0.5798125616231715 1101 3 0.530492289861602 1101 4 0.4171153453353824 1101 5 0.4171608772720674 1101 6 0.39390658014701074 1101 7 0.4807686124428656 1101 8 0.4075509084587381 1101 9 0.5535221312910963 1101 10-11 0.5707153502106124 1101 12-13 0.5902879085370145 1101 14-15 0.6707627567249261 1101 16-17 0.7335301441775783 1101 18-19 0.7640897684062438 1101 20-21 0.7093532437429175 1101 22-23 0.6917473950475426 1101 24-25 0.6294610547737989 1101 26-27 0.6374166250311504 1101 28-29 0.6392284114092632 1101 30-31 0.7923982872463355 1101 32-33 0.6770741607158541 1101 34-35 0.6622241375403846 1101 36-37 0.7706601274203209 1101 38-39 0.827819325330843 1101 40-41 0.7991641774262987 1101 42-43 0.7113344545083393 1101 44-45 0.7629738798756236 1101 46-47 0.804266912565943 1101 48-49 0.8783395488136065 1101 50-51 0.9101492572111027 1101 52-53 0.7966626750798866 1101 54-55 0.7390381605063894 1101 56-57 0.9385869222406669 1101 58-59 0.774486064497836 1101 60-61 0.8545106415970807 1101 62-63 0.8157506259219289 1101 64-65 0.9312434136518988 1101 66-67 0.8725634021430935 1101 68-69 0.7510831379799825 1101 70-71 0.674459773942921 1101 72-73 0.7069794344253459 1101 74-75 0.7008619320651057 1101 76-77 0.8564971060221751 1101 78-79 0.8036969351351395 1101 80-81 0.6907387096318658 1101 82-83 0.7859619316088633 1101 84-85 0.8824734422217944 1101 86-87 0.8724056855386415 1101 88-89 0.9163515191209797 1101 90-91 0.897793391724381 1101 92-93 0.9175878555530268 1101 94-95 1.0720124382753298 1101 96-97 1.104302029710908 1101 98-99 1.0903815784446742 1101 100-101 1.2076407052992515 1102 1 0.44594203308876956 1102 2 0.503741784690412 1102 3 0.492358266318341 1102 4 0.29636906334766167 1102 5 0.3762160753245496 1102 6 0.3997642828236252 1102 7 0.4771657011980466 1102 8 0.5241913045669264 1102 9 0.5793707062070084 1102 10-11 0.6437173955229838 1102 12-13 0.6872740205896761 1102 14-15 0.7185581181775049 1102 16-17 0.7224143003320052 1102 18-19 0.7292500198839207 1102 20-21 0.7977662969226458 1102 22-23 0.7277244991795229 1102 24-25 0.7746771089658964 1102 26-27 0.7973444864845263 1102 28-29 0.8352992815152191 1102 30-31 0.7870241615081355 1102 32-33 0.7908840995974558 1102 34-35 0.7591522974692921 1102 36-37 0.7979914815192899 1102 38-39 0.8302383345270314 1102 40-41 0.868409937162177 1102 42-43 0.8146908824791907 1102 44-45 0.8791250765247227 1102 46-47 0.8186743406547023 1102 48-49 0.7221113461214159 1102 50-51 0.746668846233753 1102 52-53 0.8191503481049267 1102 54-55 0.7237948242375651 1102 56-57 0.5852331047258446 1102 58-59 0.7637271703930324 1102 60-61 0.5903311665605955 1102 62-63 0.6411281862170384 1102 64-65 0.6939086233895893 1102 66-67 0.7189890693676233 1102 68-69 0.7221439839348065 1102 70-71 0.6708556578722451 1102 72-73 0.6106138478502565 1102 74-75 0.7483380146653964 1102 76-77 0.9067735252026168 1102 78-79 0.8992532803424851 1102 80-81 0.67429022621846 1102 82-83 0.8135263793504244 1102 84-85 0.9368017309224896 1102 86-87 0.9195510836018599 1102 88-89 0.834591152879625 1102 90-91 0.7635100880083456 1102 92-93 0.884099560195267 1102 94-95 0.7380631042548735 1102 96-97 0.9676279900454681 1102 98-99 1.0080418114483543 1102 100-101 1.1650357336415773 1103 1 0.3816840459726727 1103 2 0.49141708107011794 1103 3 0.4006840510965439 1103 4 0.33202237101124865 1103 5 0.3612083374736912 1103 6 0.4249334775192466 1103 7 0.463779700206878 1103 8 0.4683269538869652 1103 9 0.5339517559666476 1103 10-11 0.5967614648101858 1103 12-13 0.6017267418088892 1103 14-15 0.6920502768346282 1103 16-17 0.6526339594072894 1103 18-19 0.6252145219694398 1103 20-21 0.7160863919677212 1103 22-23 0.7174568738075635 1103 24-25 0.7098113584285031 1103 26-27 0.7395154943849889 1103 28-29 0.7868832523251967 1103 30-31 0.8024002114400091 1103 32-33 0.8375257916406085 1103 34-35 0.7826214768660975 1103 36-37 0.8419650735236814 1103 38-39 0.8295572965906146 1103 40-41 0.9563191068645622 1103 42-43 0.9771059592944695 1103 44-45 0.9020088743928838 1103 46-47 0.9392551387441159 1103 48-49 0.902996643460007 1103 50-51 0.8855031445098334 1103 52-53 0.8636059847886983 1103 54-55 0.9605451704780776 1103 56-57 0.9151393319657544 1103 58-59 0.904618702077876 1103 60-61 0.6789102802283367 1103 62-63 0.7108116294505038 1103 64-65 0.7395538751234341 1103 66-67 0.6992529724407603 1103 68-69 0.7973305798731403 1103 70-71 0.6998033970450663 1103 72-73 0.6760328229162802 1103 74-75 0.7316283220102839 1103 76-77 0.850580622302946 1103 78-79 1.0094406179476962 1103 80-81 1.0766771290020714 1103 82-83 0.921952064174274 1103 84-85 0.8377540203302161 1103 86-87 0.7851761737888623 1103 88-89 0.8195173942639542 1103 90-91 0.866765788369964 1103 92-93 0.9399311720235808 1103 94-95 0.9590297553124003 1103 96-97 0.9034930839439106 1103 98-99 1.1513325113491462 1103 100-101 1.3316913063371878 1104 1 0.40131999105480176 1104 2 0.4830585383881285 1104 3 0.4389227877024382 1104 4 0.24368327697619918 1104 5 0.3833976988048633 1104 6 0.3026677458699467 1104 7 0.3865519181987125 1104 8 0.46868748608159905 1104 9 0.5283042367964939 1104 10-11 0.46717134861612664 1104 12-13 0.4614712349865755 1104 14-15 0.5746058540444423 1104 16-17 0.536817847346299 1104 18-19 0.5762242241921669 1104 20-21 0.570441877604587 1104 22-23 0.5191843699572374 1104 24-25 0.5415131440562888 1104 26-27 0.6858882837812814 1104 28-29 0.5575971663452961 1104 30-31 0.6483031704605509 1104 32-33 0.8344247682872705 1104 34-35 0.7542775072749208 1104 36-37 0.7190565230171373 1104 38-39 0.7260953838590538 1104 40-41 0.7235058328504849 1104 42-43 0.7605829362442975 1104 44-45 0.7364860882000812 1104 46-47 0.8262155710612902 1104 48-49 0.7712728017450274 1104 50-51 0.7069001833238602 1104 52-53 0.75235679989283 1104 54-55 0.6999989340658388 1104 56-57 0.8132039326378333 1104 58-59 0.8309303862900776 1104 60-61 0.8670561825846121 1104 62-63 0.772599815558344 1104 64-65 0.8624158801423079 1104 66-67 0.9359240525572616 1104 68-69 0.8166069973395125 1104 70-71 0.7745935126140537 1104 72-73 0.8001857117837439 1104 74-75 0.9634767538291875 1104 76-77 1.147541391712135 1104 78-79 1.069690124085561 1104 80-81 1.041739661804364 1104 82-83 1.0707945995616832 1104 84-85 1.0747519234392513 1104 86-87 1.02839833595959 1104 88-89 1.0780632909052912 1104 90-91 0.9671464344659775 1104 92-93 1.0421701774101315 1104 94-95 1.072213982352583 1104 96-97 1.1139463298387007 1104 98-99 1.2765033734720355 1104 100-101 1.4307511544582567 1105 1 0.2561496718586298 1105 2 0.3335729288160074 1105 3 0.40733856706985705 1105 4 0.19374074664361274 1105 5 0.23965181980405248 1105 6 0.24410129206247433 1105 7 0.2692248641248298 1105 8 0.24929656035538983 1105 9 0.42437220296363165 1105 10-11 0.34760929468986745 1105 12-13 0.34066351046121923 1105 14-15 0.45217708256677014 1105 16-17 0.36600999256987166 1105 18-19 0.44825222035757406 1105 20-21 0.45741108277781706 1105 22-23 0.4850874252421562 1105 24-25 0.5289863195716293 1105 26-27 0.6090149332663799 1105 28-29 0.586633386563399 1105 30-31 0.6035750608898738 1105 32-33 0.5718167177869091 1105 34-35 0.6412676249813742 1105 36-37 0.5219165326383575 1105 38-39 0.41157996560549037 1105 40-41 0.497159825508966 1105 42-43 0.43786806828703817 1105 44-45 0.47679236925410606 1105 46-47 0.45888301749770477 1105 48-49 0.5165199039481223 1105 50-51 0.47914894782007167 1105 52-53 0.4173455794866001 1105 54-55 0.4686995146382813 1105 56-57 0.516983869399489 1105 58-59 0.4514601333193369 1105 60-61 0.40434391675432835 1105 62-63 0.39543468093373235 1105 64-65 0.512970814834425 1105 66-67 0.3989823520687352 1105 68-69 0.44832563707836215 1105 70-71 0.4606856794303624 1105 72-73 0.433768280543287 1105 74-75 0.6945000409841704 1105 76-77 0.675667663469131 1105 78-79 0.6435894446380814 1105 80-81 0.6549123579378744 1105 82-83 0.7917530969390043 1105 84-85 0.7392123540319275 1105 86-87 0.7406743330604932 1105 88-89 0.7475951917083883 1105 90-91 0.7047497163298786 1105 92-93 0.7219182848620598 1105 94-95 0.6556034321894195 1105 96-97 0.520726940143124 1105 98-99 0.7384789609477878 1105 100-101 1.118203395731868 1106 1 0.09524588625504293 1106 2 0.1526136326316916 1106 3 0.1428921405406065 1106 4 -0.005722639808837471 1106 5 0.04665466814724084 1106 6 0.03548318074611245 1106 7 0.0659744375212199 1106 8 0.12223001864175131 1106 9 0.15108780017771295 1106 10-11 0.115000504430121 1106 12-13 0.07048990574658376 1106 14-15 0.21096984926233375 1106 16-17 0.1515025260911358 1106 18-19 0.1817899896361439 1106 20-21 0.1113246392801912 1106 22-23 0.1729355811145794 1106 24-25 0.10486331463716425 1106 26-27 0.20203068281273318 1106 28-29 0.26400366329097835 1106 30-31 0.23077698481725406 1106 32-33 0.33626585548142884 1106 34-35 0.2696191181926153 1106 36-37 0.37242633029669037 1106 38-39 0.38462038464319903 1106 40-41 0.4065968707021881 1106 42-43 0.40843449620215466 1106 44-45 0.3731641681777518 1106 46-47 0.2042515726001355 1106 48-49 0.40462535996753246 1106 50-51 0.33808103320051686 1106 52-53 0.21866086082527403 1106 54-55 0.2095395787034704 1106 56-57 0.2515901898933066 1106 58-59 0.4018790822942222 1106 60-61 0.16201633841502883 1106 62-63 0.1721740941502432 1106 64-65 0.06486452504367435 1106 66-67 0.20025742365758248 1106 68-69 0.1649490800244564 1106 70-71 0.1639435001210856 1106 72-73 0.21130495239848557 1106 74-75 0.36734633354664936 1106 76-77 0.468762938182703 1106 78-79 0.3640042937677208 1106 80-81 0.3277344260626265 1106 82-83 0.46941371481990757 1106 84-85 0.4821501558163135 1106 86-87 0.3435461190268825 1106 88-89 0.564132820992242 1106 90-91 0.46222211032965177 1106 92-93 0.5537672023958748 1106 94-95 0.5787761560922355 1106 96-97 0.4483512213674352 1106 98-99 0.5289651087946012 1106 100-101 0.8286855564052402 1107 1 0.04972038364405762 1107 2 0.07313367445405206 1107 3 0.1362979348984723 1107 4 -0.03815292859475505 1107 5 0.06296082568395889 1107 6 0.10878832184803855 1107 7 0.13805344955048326 1107 8 0.033069174389268596 1107 9 0.03390655733134196 1107 10-11 0.06287036000318125 1107 12-13 0.007001320979121317 1107 14-15 0.06446951093614217 1107 16-17 0.12975887929398056 1107 18-19 0.11882831029559782 1107 20-21 0.15690208005853634 1107 22-23 0.1373283268573502 1107 24-25 0.15404487590819116 1107 26-27 0.30885705691704857 1107 28-29 0.2892861438190195 1107 30-31 0.11876577393955756 1107 32-33 0.33378471583183256 1107 34-35 0.12558948334219622 1107 36-37 0.17502950036943332 1107 38-39 0.14472357170506456 1107 40-41 0.09251148241341411 1107 42-43 0.2587132633939362 1107 44-45 0.34587648606652266 1107 46-47 0.3102716916016348 1107 48-49 0.46148139222162143 1107 50-51 0.3897282012112626 1107 52-53 0.28563706926139076 1107 54-55 0.4461786064986413 1107 56-57 0.5145786149630354 1107 58-59 0.49112584295441053 1107 60-61 0.37924942234807446 1107 62-63 0.31715487702831524 1107 64-65 0.24004953656229588 1107 66-67 0.37297367331416353 1107 68-69 0.409374949697515 1107 70-71 0.22983458918192667 1107 72-73 0.17091064948150247 1107 74-75 0.4132679253592535 1107 76-77 0.46465715324166723 1107 78-79 0.5723746391128479 1107 80-81 0.6326282149373768 1107 82-83 0.5992576770322202 1107 84-85 0.49109419947932054 1107 86-87 0.48403594933954963 1107 88-89 0.5711374352372758 1107 90-91 0.6149271180427149 1107 92-93 0.6849824293074178 1107 94-95 0.6712792456850885 1107 96-97 0.5907309699478738 1107 98-99 0.5619674455267294 1107 100-101 0.8365095316457847 1108 1 0.24546069300246032 1108 2 0.36110478241732835 1108 3 0.3330729074367049 1108 4 0.16101237111889333 1108 5 0.24569520290801705 1108 6 0.1660280682279449 1108 7 0.28646037256624624 1108 8 0.3226403781619567 1108 9 0.32094313080961 1108 10-11 0.2519543198771004 1108 12-13 0.38353748658020237 1108 14-15 0.4832673481563319 1108 16-17 0.4510576269981357 1108 18-19 0.3808430174338824 1108 20-21 0.4586310670211944 1108 22-23 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-0.6424191405653836 2316 78-79 -0.337131124351032 2316 80-81 -0.5227205635458709 2316 82-83 -0.8361735428635697 2316 84-85 -0.7137471403208977 2316 86-87 -0.6843388971880024 2316 88-89 -0.980893240496961 2316 90-91 -0.6754658584955102 2316 92-93 -0.8109150085651287 2316 94-95 -0.5379991474709591 2316 96-97 -0.3897199225534287 2316 98-99 -0.2861694341448455 2316 100-101 -0.46051131113479826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 24.0 4 54.0 5 101.0 6 232.0 7 674.0 8 1456.0 9 2719.0 10 4403.0 11 6786.0 12 9342.0 13 12075.0 14 14366.0 15 17745.0 16 20402.0 17 22984.0 18 21703.0 19 19701.0 20 20476.0 21 21949.0 22 24328.0 23 28735.0 24 35278.0 25 43995.0 26 56009.0 27 71521.0 28 90177.0 29 111564.0 30 134176.0 31 161652.0 32 193146.0 33 232716.0 34 284243.0 35 353606.0 36 452386.0 37 570982.0 38 465389.0 39 75297.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.673996813837306 21.181002072178366 14.37645634605323 23.7685447679311 2 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21.306206054508948 20-21 22.35263527095224 27.095126452195316 29.047200203437846 21.5050380734146 22-23 22.122443260049042 26.926446477428584 28.87335748182408 22.07775278069829 24-25 22.04719848660243 27.143889171597944 28.889337687580095 21.919574654219527 26-27 22.057555467035215 27.25088789407409 28.790783599494524 21.900773039396174 28-29 22.05442582479929 27.36186211711255 28.773660352773 21.810051705315153 30-31 22.325599638097625 27.154095904967406 28.71539312008618 21.80491133684879 32-33 22.160566402754505 27.259961341526644 28.646707733732857 21.932764521985995 34-35 22.26934879115814 27.283305529665796 28.70074120003802 21.74660447913804 36-37 22.270518556357857 27.204030261856758 28.663820807154984 21.861630374630398 38-39 22.146718782602992 27.290103903880013 28.675391270790023 21.887786042726972 40-41 22.250212849107427 27.13810766675506 28.731879911231456 21.87979957290605 42-43 22.184378659208193 27.217327497536743 28.492164400403162 22.106129442851902 44-45 22.147377277841652 27.375234374138525 28.449729791899912 22.027658556119913 46-47 22.263238770702078 27.333073061954128 28.23780689771265 22.165881269631146 48-49 22.238376657882693 27.444245910524618 28.17469905500739 22.142678376585298 50-51 22.25210864263821 27.497217374767878 28.06554410810569 22.185129874488222 52-53 22.328522008161087 27.507415555113052 28.033451477651116 22.130610959074737 54-55 22.204836546418406 27.44328848080245 28.087403805671542 22.264471167107597 56-57 22.22739769967785 27.5843658271273 27.971544893048378 22.21669158014647 58-59 22.23426016160061 27.546900151504932 28.03448555538013 22.184354131514326 60-61 22.281539813290628 27.61050155032568 28.050365615446616 22.057593020937077 62-63 22.27851291428527 27.526385912648937 28.021406854714275 22.173694318351526 64-65 22.04731152870987 27.457700382857848 28.073452711662945 22.42153537676934 66-67 22.08923423200006 27.52894709083047 27.969927858435717 22.411890818733752 68-69 22.101520498033626 27.51951809471038 28.006539632270027 22.372421774985963 70-71 22.126988189328376 27.35376638384236 27.98893364208569 22.53031178474358 72-73 22.11847730733621 27.483994469731222 27.891655559619878 22.505872663312694 74-75 22.08218177697475 27.621082172683437 27.813683252575743 22.483052797766067 76-77 22.072282215487824 27.507312357222354 27.825561036295078 22.594844390994755 78-79 22.007576392029435 27.532424389617493 27.828556912271296 22.631442306081773 80-81 22.055969292034682 27.707973413302227 27.707470955866654 22.52858633879644 82-83 22.17666488667859 27.556629999656657 27.78944741558851 22.477257698076244 84-85 22.167875044261276 27.565496568590987 27.61878456200214 22.647843825145596 86-87 21.952919547981658 27.671496454492644 27.72326312289929 22.652320874626405 88-89 22.011285963698505 27.513189777453167 27.70426202774693 22.7712622311014 90-91 22.067679846038274 27.48172974681124 27.737439314341035 22.713151092809454 92-93 22.08263186842333 27.41936294422621 27.73935657869389 22.758648608656564 94-95 21.979249145500095 27.37855521718443 27.711729577037026 22.930466060278444 96-97 21.872652600015464 27.330888803968044 27.77301997727235 23.02343861874414 98-99 21.79948978637631 27.34779078170879 27.687547052677502 23.165172379237394 100-101 21.81993685275963 27.335034045412304 27.633896183614375 23.21113291821369 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 842.0 1 620.5 2 515.0 3 662.0 4 566.0 5 532.0 6 865.0 7 1850.0 8 2927.0 9 3198.5 10 2604.0 11 1958.5 12 1930.5 13 2089.5 14 2304.0 15 2577.5 16 2804.0 17 3109.5 18 3489.5 19 3914.0 20 4547.5 21 5544.5 22 6865.5 23 8678.5 24 11051.0 25 14319.5 26 19005.0 27 24557.5 28 31087.5 29 39507.5 30 48612.0 31 57606.5 32 67429.0 33 75789.5 34 81985.5 35 88147.5 36 96515.5 37 105003.5 38 109938.0 39 112559.0 40 114324.0 41 116155.0 42 118596.0 43 122614.0 44 126368.0 45 129554.0 46 132331.0 47 133836.5 48 135381.0 49 134749.5 50 131031.5 51 127053.0 52 124080.5 53 120741.5 54 115407.0 55 106372.0 56 95400.0 57 84529.5 58 73729.5 59 63222.5 60 52679.5 61 42735.5 62 34177.5 63 26348.5 64 19704.5 65 14671.5 66 10541.5 67 7904.5 68 5970.5 69 4291.5 70 3105.0 71 2216.0 72 1558.5 73 1112.0 74 792.5 75 547.5 76 363.0 77 220.5 78 134.5 79 64.0 80 38.5 81 27.0 82 22.5 83 12.5 84 8.5 85 5.0 86 2.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01906531923523226 2 0.002568095709577406 3 0.0032101196369717575 4 0.03207328228591782 5 0.0017306731955847735 6 8.374225139926324E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.3957041899877204E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.978520949938603E-5 24-25 5.024535083955794E-4 26-27 0.0018981576983833 28-29 1.1165633519901763E-4 30-31 0.006685423070041181 32-33 0.02233126703980353 34-35 0.012826521505987152 36-37 6.559809692942287E-4 38-39 6.559809692942287E-4 40-41 0.0019818999497825634 42-43 0.007718244170632095 44-45 0.0076624160030325855 46-47 0.007327446997435533 48-49 0.00919769061201908 50-51 0.18593571219016414 52-53 0.04946375649316481 54-55 0.016999677034050437 56-57 0.009979284958412202 58-59 0.019246760779930667 60-61 0.052562219794937554 62-63 0.09436356028506979 64-65 0.09901125523772888 66-67 0.10310066851439291 68-69 0.10123042489980938 70-71 0.09169776528219324 72-73 0.06940836936808935 74-75 0.23597170740122395 76-77 0.28512840897259145 78-79 0.006727294195740812 80-81 7.815943463931234E-4 82-83 0.0 84-85 1.3957041899877204E-5 86-87 0.0 88-89 1.814415446984037E-4 90-91 0.006476067441543024 92-93 0.017613786877645032 94-95 0.0017446302374846507 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3582421.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.83326426601755 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8049481158786 46.553240218211485 2 12.629720303982609 15.113547851081577 3 4.077370940846255 7.318872390427039 4 1.7655295396694366 4.225495820660069 5 0.9797442842028482 2.931064933491461 6 0.5927748167522303 2.1280591356585794 7 0.40551276147852344 1.6984206554550925 8 0.2926737180807647 1.4009299134115452 9 0.22505583349971114 1.2119242644357355 >10 1.1479077856318998 12.135833776225958 >50 0.05795482503854003 2.3516486215772776 >100 0.01968537940199575 2.021753923344497 >500 8.880089713866249E-4 0.34102659745863234 >1k 1.4021193907872974E-4 0.1904379726941684 >5k 9.347462605248646E-5 0.37774392586678807 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7510 0.20963476933615563 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5998 0.16742867463092698 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4402 0.12287779688651891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.7914083799754412E-5 2 0.0 0.0 0.0 0.0 2.7914083799754412E-5 3 0.0 0.0 0.0 0.0 2.7914083799754412E-5 4 0.0 0.0 0.0 0.0 2.7914083799754412E-5 5 0.0 2.7914083799754412E-5 0.0 2.7914083799754412E-5 2.7914083799754412E-5 6 0.0 2.7914083799754412E-5 0.0 2.7914083799754412E-5 8.374225139926324E-5 7 0.0 2.7914083799754412E-5 0.0 2.7914083799754412E-5 8.374225139926324E-5 8 0.0 2.7914083799754412E-5 0.0 5.5828167599508824E-5 8.374225139926324E-5 9 0.0 2.7914083799754412E-5 0.0 8.374225139926324E-5 8.374225139926324E-5 10-11 0.0 2.7914083799754412E-5 0.0 1.5352746089864925E-4 8.374225139926324E-5 12-13 0.0 2.7914083799754412E-5 0.0 2.0935562849815808E-4 1.9539858659828088E-4 14-15 0.0 2.7914083799754412E-5 0.0 2.233126703980353E-4 2.7914083799754406E-4 16-17 0.0 2.7914083799754412E-5 0.0 2.930978798974213E-4 3.6288308939680733E-4 18-19 0.0 2.7914083799754412E-5 0.0 3.070549217972985E-4 4.1871125699631615E-4 20-21 0.0 2.7914083799754412E-5 0.0 3.7684013129668455E-4 4.884964664957021E-4 22-23 0.0 2.7914083799754412E-5 0.0 5.303675921953338E-4 5.303675921953338E-4 24-25 0.0 2.7914083799754412E-5 0.0 7.257661787936148E-4 6.420239273943514E-4 26-27 0.0 4.187112569963162E-5 0.0 0.0010467781424907904 6.978520949938603E-4 28-29 0.0 5.5828167599508824E-5 0.0 0.0022749978296799844 7.257661787936148E-4 30-31 0.0 5.5828167599508824E-5 0.0 0.00784385754773099 7.676373044932463E-4 32-33 0.0 5.5828167599508824E-5 0.0 0.021591543819110038 8.374225139926323E-4 34-35 0.0 6.978520949938603E-5 0.0 0.04170364119683309 8.374225139926323E-4 36-37 0.0 8.374225139926324E-5 0.0 0.07104134327037498 8.374225139926323E-4 38-39 0.0 8.374225139926324E-5 0.0 0.11277289855100782 8.513795558925095E-4 40-41 0.0 8.374225139926324E-5 0.0 0.15915214878429979 8.653365977923867E-4 42-43 0.0 8.374225139926324E-5 0.0 0.206410692657284 8.653365977923867E-4 44-45 0.0 8.374225139926324E-5 0.0 0.2660072615697597 9.072077234920184E-4 46-47 0.0 8.374225139926324E-5 0.0 0.3336570436584645 9.909499748912815E-4 48-49 0.0 8.374225139926324E-5 0.0 0.40581495028082964 0.0011026063100902993 50-51 0.0 8.374225139926324E-5 0.0 0.4812806758334657 0.0012003056033894398 52-53 0.0 8.374225139926324E-5 0.0 0.5568022295537012 0.001214262645289317 54-55 0.0 9.769929329914044E-5 0.0 0.6423170252742489 0.0012700908128888257 56-57 0.0 1.1165633519901765E-4 0.0 0.7436451494673574 0.0013398760223882118 58-59 0.0 1.2561337709889487E-4 0.0 0.8555666684624728 0.001353833064288089 60-61 0.0 1.3957041899877206E-4 0.0 0.9744108802399272 0.0014515323575872293 62-63 0.0 1.3957041899877206E-4 0.0 1.0950415933805657 0.0015911027765860015 64-65 0.0 1.3957041899877206E-4 0.0 1.2211295099040564 0.0016050598184858787 66-67 0.0 1.3957041899877206E-4 0.0 1.367120168176772 0.001619016860385756 68-69 0.0 1.3957041899877206E-4 0.0 1.5332787519948101 0.0016329739022856332 70-71 0.0 1.3957041899877206E-4 0.0 1.715250664285409 0.0016469309441855104 72-73 0.0 1.3957041899877206E-4 0.0 1.9069227206964228 0.001828372488883914 74-75 0.0 1.3957041899877206E-4 0.0 2.1075970691328574 0.0018562865726836685 76-77 0.0 1.6748450279852647E-4 0.0 2.3256758488184386 0.0019121147402831771 78-79 0.0 1.6748450279852647E-4 0.0 2.5709987742925806 0.0019400288240829316 80-81 0.0 1.6748450279852647E-4 0.0 2.8430075638792873 0.001967942907882686 82-83 0.0 1.6748450279852647E-4 0.0 3.131094865734653 0.0020098140335823174 84-85 0.0 1.6748450279852647E-4 0.0 3.4231180534057835 0.002023771075482195 86-87 0.0 1.6748450279852647E-4 0.0 3.73726315248822 0.0021633414944809666 88-89 0.0 1.6748450279852647E-4 0.0 4.087138278834342 0.0023029119134797393 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3625 0.0 52.783554 1 GTATCAA 6915 0.0 42.638412 1 ATCAACG 8875 0.0 32.41313 3 TCAACGC 8920 0.0 32.195946 4 CAACGCA 8975 0.0 31.831532 5 TATCAAC 9290 0.0 31.118912 2 AACGCAG 9590 0.0 29.78978 6 ACGCAGA 11060 0.0 25.787472 7 CGCAGAG 11380 0.0 25.020638 8 GTACATG 9230 0.0 23.302263 1 TACATGG 9570 0.0 21.72626 2 ACATGGG 9550 0.0 21.075563 3 GCAGAGT 13435 0.0 20.699 9 AGAGTAC 12175 0.0 19.917797 10-11 CATGGGG 6850 0.0 19.195518 4 GAGTACA 7270 0.0 18.808739 1 GTGGTAT 2325 0.0 18.583181 1 CAGAGTA 12605 0.0 17.939465 10-11 GAGTACT 8720 0.0 17.741508 12-13 TATACCG 325 1.0153599E-8 17.52239 5 >>END_MODULE