FastQCFastQC Report
Sat 18 Jun 2016
SRR1930943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113416
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2840.2504055865133667No Hit
NNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2280.2010298370600268No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2110.18604077026169147No Hit
NNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1870.16487973478168863No Hit
NNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1660.14636382873668619No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1750.063.052321
TACTGTA250.006044898654.3506287
GGTATCA802.561137E-950.1552541
TATCAAC2100.050.155252
GCCTAAA300.00830236350.155251
AGGCCCG300.00969706748.2240525
TAGACTG404.9760897E-448.224055
ATCAACG2350.042.685323
TATAGTA500.00123041440.12422
TGGTATC500.00123041440.12422
CAACGCA2550.037.822795
TCAACGC2600.036.6306534
AACGCAG2650.035.8731236
GGAGCCG550.002383427235.0720375
GTAGAAC600.00301148833.4368363
GTGGTAT600.00301148833.4368361
TAGGACC600.00302007333.417444
GTACATG2200.031.9169771
ATAGTAT650.00445661130.864773
TACATGG2350.029.8797252
Produced by FastQC (version 0.11.5)