##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930943_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 113416 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.524864216689004 30.0 10.0 31.0 2.0 33.0 2 21.451955632362278 28.0 2.0 31.0 2.0 33.0 3 21.888534245609087 28.0 10.0 31.0 2.0 33.0 4 23.83031494674473 32.0 2.0 35.0 2.0 37.0 5 23.835243704591946 32.0 2.0 35.0 2.0 37.0 6 24.53243810397122 32.0 10.0 35.0 2.0 37.0 7 24.61023136065458 32.0 10.0 35.0 2.0 37.0 8 25.400446145164704 32.0 17.0 35.0 2.0 37.0 9 26.464378923608663 33.0 17.0 37.0 2.0 39.0 10-11 26.67669905480708 33.0 17.0 37.0 2.0 39.0 12-13 26.615675918741623 33.0 17.0 37.0 2.0 39.0 14-15 26.807857797841574 33.5 14.0 38.0 2.0 40.0 16-17 25.973729456161387 33.0 10.0 38.0 2.0 40.0 18-19 25.40773788530719 32.0 10.0 38.0 2.0 40.0 20-21 24.972781618113846 32.0 8.0 38.0 2.0 40.0 22-23 24.890703251745784 32.0 10.0 38.0 2.0 40.0 24-25 24.618638463708823 32.0 5.0 38.0 2.0 40.0 26-27 24.27836019609226 31.5 5.0 37.5 2.0 40.0 28-29 24.185295725470834 31.0 5.0 37.0 2.0 40.0 30-31 24.10973760316005 31.0 2.0 37.0 2.0 39.0 32-33 23.79254690696198 30.5 2.0 37.0 2.0 39.0 34-35 20.92999224095366 25.0 2.0 36.0 2.0 39.0 36-37 19.330429569020247 18.0 2.0 35.0 2.0 38.5 38-39 18.39264301333145 17.0 2.0 34.0 2.0 38.0 40-41 19.73375449672004 23.0 2.0 34.5 2.0 38.0 42-43 19.66794367637723 22.5 2.0 35.0 2.0 38.0 44-45 17.69551915073711 15.5 2.0 34.0 2.0 38.0 46-47 18.5335093813924 18.5 2.0 33.0 2.0 38.0 48-49 17.98669499894195 16.0 2.0 33.0 2.0 38.0 50-51 15.995538548352966 10.0 2.0 31.5 2.0 37.5 52-53 18.884967729420893 21.5 2.0 33.5 2.0 37.5 54-55 20.543433025322706 26.0 2.0 35.5 2.0 38.5 56-57 20.739613458418567 27.0 2.0 36.0 2.0 39.0 58-59 20.379840586866052 26.0 2.0 36.0 2.0 39.0 60-61 20.08771690061367 26.0 2.0 35.0 2.0 39.0 62-63 19.60584485434154 25.5 2.0 35.0 2.0 38.0 64-65 19.098068173802638 23.5 2.0 34.0 2.0 38.0 66-67 18.661132468082105 22.0 2.0 34.0 2.0 38.0 68-69 18.214370106510543 21.5 2.0 34.0 2.0 37.0 70-71 17.59518057416943 19.0 2.0 33.0 2.0 36.0 72-73 17.11653999435706 17.0 2.0 32.5 2.0 36.0 74-75 16.520795125908162 10.0 2.0 32.0 2.0 35.0 76-77 15.965379664244903 7.5 2.0 31.5 2.0 35.0 78-79 15.4266020667278 4.0 2.0 31.0 2.0 35.0 80-81 14.932372857445158 2.0 2.0 31.0 2.0 34.0 82-83 14.42990406997249 2.0 2.0 30.0 2.0 34.0 84-85 13.902989877971361 2.0 2.0 30.0 2.0 34.0 86-87 13.317217147492418 2.0 2.0 29.0 2.0 34.0 88-89 12.752305671157508 2.0 2.0 29.0 2.0 34.0 90-91 12.144362347464202 2.0 2.0 28.5 2.0 34.0 92-93 11.550394124285814 2.0 2.0 27.0 2.0 33.5 94-95 10.861456937292798 2.0 2.0 25.5 2.0 33.0 96-97 10.124440114269591 2.0 2.0 24.5 2.0 32.5 98-99 9.3150393242576 2.0 2.0 19.0 2.0 32.0 100-101 8.272188227410595 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.82922325369297 1101 2 7.95511250762063 1101 3 7.731342137750097 1101 4 9.462664176496876 1101 5 9.178560393529086 1101 6 8.414253969409199 1101 7 8.124666463048865 1101 8 7.102634688064242 1101 9 7.607788191728606 1101 10-11 6.15023833487345 1101 12-13 6.427627179621698 1101 14-15 7.509540889400288 1101 16-17 7.823950045108546 1101 18-19 7.564087131822081 1101 20-21 8.589265326313651 1101 22-23 8.249850432475966 1101 24-25 8.898646506974519 1101 26-27 8.852633410231721 1101 28-29 8.35752653439004 1101 30-31 8.058086365313653 1101 32-33 8.09361365274448 1101 34-35 11.359157927070473 1101 36-37 5.048631529247423 1101 38-39 8.301158209167475 1101 40-41 8.97428633363251 1101 42-43 9.210940435405519 1101 44-45 4.0012190618009775 1101 46-47 5.488691102146269 1101 48-49 8.51437742897135 1101 50-51 -4.1765947966917025 1101 52-53 4.752485435307033 1101 54-55 6.669727208530901 1101 56-57 8.166378387734259 1101 58-59 8.20096584619873 1101 60-61 7.635966860272241 1101 62-63 7.6530385532153 1101 64-65 8.08714167918555 1101 66-67 7.517305109759462 1101 68-69 8.187912067132416 1101 70-71 8.537347677816033 1101 72-73 8.099542853205346 1101 74-75 7.85033898035481 1101 76-77 7.264087758009742 1101 78-79 7.806917307360937 1101 80-81 7.64253075812789 1101 82-83 7.0304735979784905 1101 84-85 7.131441522817804 1101 86-87 7.56222534534769 1101 88-89 7.623928781886962 1101 90-91 6.427038307722386 1101 92-93 6.786038172466061 1101 94-95 5.525760649081139 1101 96-97 5.399401045321309 1101 98-99 5.98954100567766 1101 100-101 5.936190960609382 1102 1 6.133191507661223 1102 2 7.432096634604758 1102 3 7.417850074258034 1102 4 8.52377528760799 1102 5 9.004354044322735 1102 6 7.87933333448856 1102 7 7.822285510667911 1102 8 7.00501564044519 1102 9 6.827232636173047 1102 10-11 6.5161113507464705 1102 12-13 6.026436703431219 1102 14-15 6.4652948576542535 1102 16-17 8.217997664156165 1102 18-19 8.529364909599867 1102 20-21 9.386289135837465 1102 22-23 8.598064718190251 1102 24-25 8.667297300625318 1102 26-27 8.160966743565059 1102 28-29 7.85038367724718 1102 30-31 7.954514936742225 1102 32-33 8.468812065442894 1102 34-35 10.979991260403803 1102 36-37 1.5910918467077408 1102 38-39 3.239253447262719 1102 40-41 8.65960379394997 1102 42-43 10.00816265762774 1102 44-45 1.6591555697374858 1102 46-47 1.8960323719875376 1102 48-49 7.471917111511033 1102 50-51 -4.825205907802815 1102 52-53 4.259628292449889 1102 54-55 6.75206847837217 1102 56-57 7.879672038527911 1102 58-59 8.685489655722538 1102 60-61 8.10283193963732 1102 62-63 8.137959188135937 1102 64-65 7.782975012518882 1102 66-67 7.822463839918193 1102 68-69 8.56529301951337 1102 70-71 8.4087762492446 1102 72-73 8.90053491669741 1102 74-75 8.701529456545288 1102 76-77 8.445238551660536 1102 78-79 7.845607783551415 1102 80-81 8.480030758127892 1102 82-83 7.4876164551213495 1102 84-85 7.240568506944788 1102 86-87 8.447145980268326 1102 88-89 6.387817670775847 1102 90-91 6.84926052994461 1102 92-93 8.457069918497808 1102 94-95 7.902149537970029 1102 96-97 8.089083585003848 1102 98-99 7.554818783455438 1102 100-101 6.685595722514144 1103 1 5.940718389381654 1103 2 7.299838570088628 1103 3 6.716237171032226 1103 4 7.506570986532719 1103 5 7.805160495935642 1103 6 7.104602151692863 1103 7 7.045941424646404 1103 8 5.885660801735515 1103 9 6.6893294103666 1103 10-11 5.8650360819292615 1103 12-13 5.3963291765495 1103 14-15 6.1592464705574805 1103 16-17 7.090847126521751 1103 18-19 6.921165984868679 1103 20-21 7.504971931536389 1103 22-23 6.274812030018204 1103 24-25 7.1761682683672525 1103 26-27 7.087848463995169 1103 28-29 7.313555720257934 1103 30-31 8.01123536684975 1103 32-33 7.475398086948271 1103 34-35 9.653512765780153 1103 36-37 -3.370467293077205 1103 38-39 -2.601875584995348 1103 40-41 5.444012396100508 1103 42-43 7.542033625369676 1103 44-45 0.24571470952243146 1103 46-47 0.49105925370796655 1103 48-49 6.417347219037914 1103 50-51 -5.309211284146901 1103 52-53 3.801832593525159 1103 54-55 6.70435342460873 1103 56-57 7.172010748205331 1103 58-59 7.354575677227913 1103 60-61 7.364525488024416 1103 62-63 7.232851661254216 1103 64-65 6.6650986684328615 1103 66-67 7.349614377552605 1103 68-69 7.944056460373584 1103 70-71 7.903131087954282 1103 72-73 7.356583303794185 1103 74-75 7.541583219986144 1103 76-77 6.88838371295086 1103 78-79 7.2239679986051755 1103 80-81 6.7782834462999375 1103 82-83 7.14568097125038 1103 84-85 6.840030872536184 1103 86-87 6.062737378117786 1103 88-89 5.950989713786603 1103 90-91 5.772916443923103 1103 92-93 5.256532284089204 1103 94-95 4.5060473874323925 1103 96-97 3.771072832315676 1103 98-99 3.0509209339930727 1103 100-101 2.792181744019519 1104 1 6.172524840994559 1104 2 6.538096634604756 1104 3 6.147850074258034 1104 4 8.100441954274654 1104 5 8.597354044322739 1104 6 7.128666667821893 1104 7 7.717618844001244 1104 8 6.25501564044519 1104 9 5.764232636173048 1104 10-11 6.4407780174131375 1104 12-13 5.836103370097888 1104 14-15 6.2237948576542585 1104 16-17 6.614330997489496 1104 18-19 7.399198242933199 1104 20-21 7.453455802504131 1104 22-23 7.706231384856913 1104 24-25 7.546297300625316 1104 26-27 7.153300076898393 1104 28-29 7.401717010580512 1104 30-31 7.514848270075557 1104 32-33 6.936978732109562 1104 34-35 10.03115792707047 1104 36-37 -2.723241486625593 1104 38-39 -2.032746552737283 1104 40-41 6.365270460616639 1104 42-43 8.699162657627738 1104 44-45 0.41648890307081743 1104 46-47 -0.09813429467913082 1104 48-49 6.948250444844366 1104 50-51 -5.1523725744694815 1104 52-53 4.122961625783223 1104 54-55 6.844901811705505 1104 56-57 7.3031720385279115 1104 58-59 8.075156322389205 1104 60-61 7.951331939637317 1104 62-63 7.876625854802601 1104 64-65 7.733808345852214 1104 66-67 6.876130506584865 1104 68-69 7.036959686180037 1104 70-71 7.384776249244602 1104 72-73 6.663034916697409 1104 74-75 7.223196123211949 1104 76-77 7.027738551660535 1104 78-79 7.093774450218078 1104 80-81 6.911864091461226 1104 82-83 7.039616455121349 1104 84-85 6.93690184027812 1104 86-87 6.606479313601659 1104 88-89 6.936151004109183 1104 90-91 6.800593863277941 1104 92-93 6.381403251831138 1104 94-95 5.617982871303358 1104 96-97 4.889750251670513 1104 98-99 5.137985450122105 1104 100-101 5.0047623891808115 1105 1 6.1929476052222014 1105 2 7.512990943547845 1105 3 7.285736253119822 1105 4 8.43149886484376 1105 5 9.447240223184526 1105 6 8.169577236927584 1105 7 8.103179819610997 1105 8 7.1037961282500675 1105 9 7.596948083327522 1105 10-11 6.576680456437522 1105 12-13 6.384363532699513 1105 14-15 5.776575345459133 1105 16-17 6.786493599115509 1105 18-19 8.248368974640517 1105 20-21 8.96789482689438 1105 22-23 8.46645902713334 1105 24-25 8.20652494290174 1105 26-27 8.81808056470327 1105 28-29 8.467863352043928 1105 30-31 8.483173473327586 1105 32-33 9.404686049182732 1105 34-35 10.916068496176166 1105 36-37 -2.385940673617462 1105 38-39 -1.5948928942006972 1105 40-41 6.6134655825678585 1105 42-43 7.992105747058634 1105 44-45 -3.1432834546527584 1105 46-47 -3.7922806361425447 1105 48-49 4.987567518015098 1105 50-51 -9.293803468778425 1105 52-53 0.49174211358809927 1105 54-55 5.48834896617705 1105 56-57 6.473676103568561 1105 58-59 7.142603476860749 1105 60-61 7.455169338011306 1105 62-63 8.094869757241629 1105 64-65 7.115596963738398 1105 66-67 6.156000425284049 1105 68-69 5.6382604991881635 1105 70-71 6.141296574447853 1105 72-73 5.451652802876271 1105 74-75 6.023683928090001 1105 76-77 6.344323917514194 1105 78-79 7.425384206315641 1105 80-81 6.479929132111632 1105 82-83 6.741274991706712 1105 84-85 5.900121352473244 1105 86-87 5.64430045181304 1105 88-89 5.86444368703601 1105 90-91 5.236658903928349 1105 92-93 3.714996747766099 1105 94-95 4.099608887563523 1105 96-97 4.280546999637995 1105 98-99 2.702278133048935 1105 100-101 1.6086648282052014 1106 1 4.121249250443377 1106 2 5.94036435114019 1106 3 5.502495743549371 1106 4 6.693197859786466 1106 5 6.207306800228249 1106 6 5.010304463097484 1106 7 5.296957426678411 1106 8 4.732180994775899 1106 9 5.9183743684565115 1106 10-11 4.608368568594237 1106 12-13 4.375977385845918 1106 14-15 4.357716117496775 1106 16-17 4.813732572292643 1106 18-19 4.812993518523747 1106 20-21 5.648132967858466 1106 22-23 6.048688077770301 1106 24-25 5.536785489601694 1106 26-27 5.635441809181859 1106 28-29 5.495134333415159 1106 30-31 5.470722285823587 1106 32-33 4.602112590377278 1106 34-35 7.162307533369688 1106 36-37 -1.0223595968618149 1106 38-39 -0.1497229306900394 1106 40-41 5.435916129907973 1106 42-43 5.954816200934829 1106 44-45 -3.990188262283512 1106 46-47 -4.532150042710626 1106 48-49 3.1267071377577516 1106 50-51 -9.56657729887893 1106 52-53 -0.24207774429551776 1106 54-55 3.4500671660362165 1106 56-57 4.555361014905866 1106 58-59 4.671250810578183 1106 60-61 5.0499776089286605 1106 62-63 4.318799083149063 1106 64-65 4.1317611017577285 1106 66-67 5.049957278238406 1106 68-69 4.588455749172162 1106 70-71 3.829248690189484 1106 72-73 4.04098767260292 1106 74-75 4.404613446046596 1106 76-77 4.28411650441644 1106 78-79 4.351349253367687 1106 80-81 3.2829507056344553 1106 82-83 3.2222621244126888 1106 84-85 2.9134687694119794 1106 86-87 2.636778526200084 1106 88-89 3.0252848623768998 1106 90-91 2.21684583178188 1106 92-93 2.78272608647681 1106 94-95 2.3641246035868253 1106 96-97 2.231734503639018 1106 98-99 2.6032767887047825 1106 100-101 2.392321444298922 1107 1 5.218850422389906 1107 2 7.012329192744293 1107 3 6.551571004490594 1107 4 8.352845055049848 1107 5 8.550284276880877 1107 6 6.76382945851957 1107 7 7.695541324621399 1107 8 7.024007888507207 1107 9 5.997969070281577 1107 10-11 6.011072591056546 1107 12-13 4.992134377849826 1107 14-15 5.3712057103674375 1107 16-17 6.526330997489495 1107 18-19 6.5201594832432725 1107 20-21 7.332897662969252 1107 22-23 7.896029834469317 1107 24-25 7.2019872231059345 1107 26-27 7.765424107906146 1107 28-29 7.763949568720051 1107 30-31 7.369825014261604 1107 32-33 8.277629894900254 1107 34-35 9.42504939993869 1107 36-37 0.8157042498085154 1107 38-39 0.915222439510778 1107 40-41 5.95010766991896 1107 42-43 7.299441727395184 1107 44-45 -3.42689094189042 1107 46-47 -3.8674366202605253 1107 48-49 4.276374475852119 1107 50-51 -9.417550869043126 1107 52-53 0.5398608505894238 1107 54-55 5.094607238062096 1107 56-57 5.950892968760467 1107 58-59 6.128706709986101 1107 60-61 6.250215660567552 1107 62-63 6.080594847050666 1107 64-65 5.858459508642916 1107 66-67 5.493587870925946 1107 68-69 5.49106821331182 1107 70-71 5.717303381027548 1107 72-73 5.337453521348575 1107 74-75 6.107537208483272 1107 76-77 5.6488238229783665 1107 78-79 4.690084527737458 1107 80-81 4.964236184484484 1107 82-83 5.577538935741503 1107 84-85 5.580684786014553 1107 86-87 4.6583862903458435 1107 88-89 4.840337050620812 1107 90-91 5.0426713826577885 1107 92-93 5.52431798051331 1107 94-95 6.610385972078554 1107 96-97 5.772416918337182 1107 98-99 4.58514824081978 1107 100-101 4.0004213039094925 1108 1 5.900131398371606 1108 2 7.106686798539183 1108 3 6.717030402126888 1108 4 8.937163265750062 1108 5 7.886731093503066 1108 6 8.202830602248124 1108 7 6.940045073509442 1108 8 6.292720558477978 1108 9 5.675183455845179 1108 10-11 6.28359768954428 1108 12-13 5.695972222556904 1108 14-15 6.297466988801798 1108 16-17 6.190265423719005 1108 18-19 6.597329390474179 1108 20-21 7.044963999225445 1108 22-23 7.521903516004457 1108 24-25 6.70459238259253 1108 26-27 6.584873847390195 1108 28-29 7.096012092547728 1108 30-31 7.024323679911621 1108 32-33 6.841011518994804 1108 34-35 9.93702677952949 1108 36-37 -2.913962798101 1108 38-39 -1.9115662248684302 1108 40-41 6.47422128028877 1108 42-43 9.584802001890036 1108 44-45 -2.2435766706996727 1108 46-47 -3.449117901236505 1108 48-49 5.046643887467315 1108 50-51 -9.462569295780956 1108 52-53 1.8446009700455157 1108 54-55 6.002410008426818 1108 56-57 7.202417940167255 1108 58-59 7.681254683044944 1108 60-61 7.12926636586683 1108 62-63 7.462822576114078 1108 64-65 6.766103427819431 1108 66-67 7.414130506584861 1108 68-69 6.999582636999708 1108 70-71 6.268612314818373 1108 72-73 7.212215244566263 1108 74-75 7.308769893703754 1108 76-77 7.397902486086766 1108 78-79 7.269446581365621 1108 80-81 7.594716550477621 1108 82-83 7.108927930531182 1108 84-85 7.014475610769923 1108 86-87 6.411626854585263 1108 88-89 5.505987069682954 1108 90-91 5.708003699343516 1108 92-93 5.582616366585238 1108 94-95 5.2520812319590995 1108 96-97 5.421324022162317 1108 98-99 5.679887089466366 1108 100-101 4.729516487541467 1109 1 4.475846874892863 1109 2 6.28548646511323 1109 3 5.1085280403597295 1109 4 7.308238564444146 1109 5 5.447998112119347 1109 6 3.570293786465964 1109 7 2.225957827052092 1109 8 3.1058630980723088 1109 9 2.603080093800166 1109 10-11 2.298879712328386 1109 12-13 3.21132370908094 1109 14-15 3.706608416976291 1109 16-17 3.6110767602013567 1109 18-19 3.5438423107298043 1109 20-21 4.4086761414871845 1109 22-23 6.282739859433185 1109 24-25 5.840602385371081 1109 26-27 4.595571263339071 1109 28-29 5.316767858038141 1109 30-31 5.22556013448234 1109 32-33 3.846707545668881 1109 34-35 7.11763250334166 1109 36-37 -4.31893640187983 1109 38-39 -2.7446448578220313 1109 40-41 4.181778935192906 1109 42-43 5.056044013559941 1109 44-45 -3.640561944386807 1109 46-47 -5.721693616713029 1109 48-49 1.9709284109460619 1109 50-51 -9.615389523622024 1109 52-53 -0.3960214250642373 1109 54-55 2.883071303230931 1109 56-57 4.228188987680454 1109 58-59 4.389020729168866 1109 60-61 5.088213295569524 1109 62-63 4.9334394141246385 1109 64-65 5.152113430597982 1109 66-67 5.566672879466218 1109 68-69 6.459502059061393 1109 70-71 6.629962689922568 1109 72-73 6.0443908489008 1109 74-75 4.878789343550935 1109 76-77 5.231467365219858 1109 78-79 5.996384619709605 1109 80-81 5.497050532139191 1109 82-83 5.7054130652908395 1109 84-85 4.745512009769646 1109 86-87 5.000818296652506 1109 88-89 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-11.245542604166324 2313 86-87 -10.663965130842787 2313 88-89 -10.107552699594521 2313 90-91 -9.48962835894428 2313 92-93 -9.00496711853923 2313 94-95 -8.50201712869664 2313 96-97 -7.894249748329486 2313 98-99 -7.066014549877895 2313 100-101 -6.135237610819189 2314 1 -20.08347515900544 2314 2 -18.917903365395244 2314 3 -19.332149925741966 2314 4 -21.259558045725345 2314 5 -21.170645955677262 2314 6 -21.887333332178105 2314 7 -21.994381155998756 2314 8 -22.94498435955481 2314 9 -23.94776736382695 2314 10-11 -24.167221982586867 2314 12-13 -24.131896629902112 2314 14-15 -24.272205142345744 2314 16-17 -23.473669002510505 2314 18-19 -22.824801757066805 2314 20-21 -22.434544197495867 2314 22-23 -22.297768615143085 2314 24-25 -22.057702699374683 2314 26-27 -21.730699923101607 2314 28-29 -21.666282989419486 2314 30-31 -21.537151729924442 2314 32-33 -21.32702126789044 2314 34-35 -18.640842072929527 2314 36-37 -16.967241486625593 2314 38-39 -15.960746552737284 2314 40-41 -17.398729539383364 2314 42-43 -17.31683734237226 2314 44-45 -15.40751109692918 2314 46-47 -16.15813429467913 2314 48-49 -15.719749555155634 2314 50-51 -13.704372574469481 2314 52-53 -16.557038374216777 2314 54-55 -18.227098188294494 2314 56-57 -18.43282796147209 2314 58-59 -18.072843677610795 2314 60-61 -17.78466806036268 2314 62-63 -17.295374145197396 2314 64-65 -16.746191654147783 2314 66-67 -16.33586949341514 2314 68-69 -15.943040313819964 2314 70-71 -15.391223750755398 2314 72-73 -14.83696508330259 2314 74-75 -14.256803876788048 2314 76-77 -13.692261448339464 2314 78-79 -13.05022554978192 2314 80-81 -12.524135908538774 2314 82-83 -12.112383544878652 2314 84-85 -11.55109815972188 2314 86-87 -10.969520686398342 2314 88-89 -10.403848995890817 2314 90-91 -9.767406136722057 2314 92-93 -9.13459674816886 2314 94-95 -8.50201712869664 2314 96-97 -7.894249748329486 2314 98-99 -7.066014549877895 2314 100-101 -6.135237610819189 2315 1 -20.08347515900544 2315 2 -18.917903365395244 2315 3 -19.332149925741966 2315 4 -16.468648954816253 2315 5 -21.170645955677262 2315 6 -21.887333332178105 2315 7 -21.994381155998756 2315 8 -22.94498435955481 2315 9 -19.711403727463313 2315 10-11 -18.126312891677777 2315 12-13 -15.495532993538475 2315 14-15 -15.485841505982107 2315 16-17 -19.764578093419594 2315 18-19 -22.824801757066805 2315 20-21 -22.434544197495867 2315 22-23 -22.297768615143085 2315 24-25 -22.057702699374683 2315 26-27 -21.730699923101607 2315 28-29 -21.666282989419486 2315 30-31 -21.537151729924442 2315 32-33 -21.32702126789044 2315 34-35 -18.640842072929527 2315 36-37 -16.967241486625593 2315 38-39 -15.960746552737284 2315 40-41 -17.398729539383364 2315 42-43 -17.31683734237226 2315 44-45 -15.40751109692918 2315 46-47 -16.15813429467913 2315 48-49 -15.719749555155634 2315 50-51 -13.704372574469481 2315 52-53 -16.557038374216777 2315 54-55 -18.227098188294494 2315 56-57 -18.43282796147209 2315 58-59 -18.072843677610795 2315 60-61 -17.78466806036268 2315 62-63 -17.295374145197396 2315 64-65 -16.746191654147783 2315 66-67 -16.33586949341514 2315 68-69 -15.943040313819964 2315 70-71 -15.391223750755398 2315 72-73 -14.83696508330259 2315 74-75 -14.256803876788048 2315 76-77 -13.692261448339464 2315 78-79 -13.05022554978192 2315 80-81 -12.524135908538774 2315 82-83 -12.112383544878652 2315 84-85 -11.55109815972188 2315 86-87 -10.969520686398342 2315 88-89 -10.403848995890817 2315 90-91 -9.767406136722057 2315 92-93 -9.13459674816886 2315 94-95 -8.50201712869664 2315 96-97 -7.894249748329486 2315 98-99 -7.066014549877895 2315 100-101 -6.135237610819189 2316 1 -20.08347515900544 2316 2 -18.917903365395244 2316 3 -19.332149925741966 2316 4 -21.259558045725345 2316 5 -21.170645955677262 2316 6 -21.887333332178105 2316 7 -21.994381155998756 2316 8 -22.94498435955481 2316 9 -23.94776736382695 2316 10-11 -24.167221982586867 2316 12-13 -24.131896629902112 2316 14-15 -24.272205142345744 2316 16-17 -23.473669002510505 2316 18-19 -22.824801757066805 2316 20-21 -22.434544197495867 2316 22-23 -22.297768615143085 2316 24-25 -22.057702699374683 2316 26-27 -21.730699923101607 2316 28-29 -21.666282989419486 2316 30-31 -21.537151729924442 2316 32-33 -21.32702126789044 2316 34-35 -18.640842072929527 2316 36-37 -16.967241486625593 2316 38-39 -15.960746552737284 2316 40-41 -17.398729539383364 2316 42-43 -17.31683734237226 2316 44-45 -15.40751109692918 2316 46-47 -16.15813429467913 2316 48-49 -15.719749555155634 2316 50-51 -13.704372574469481 2316 52-53 -16.557038374216777 2316 54-55 -18.227098188294494 2316 56-57 -18.43282796147209 2316 58-59 -18.072843677610795 2316 60-61 -17.78466806036268 2316 62-63 -17.295374145197396 2316 64-65 -16.746191654147783 2316 66-67 -16.33586949341514 2316 68-69 -15.943040313819964 2316 70-71 -15.391223750755398 2316 72-73 -14.83696508330259 2316 74-75 -14.256803876788048 2316 76-77 -13.692261448339464 2316 78-79 -13.05022554978192 2316 80-81 -12.524135908538774 2316 82-83 -12.112383544878652 2316 84-85 -11.55109815972188 2316 86-87 -10.969520686398342 2316 88-89 -10.403848995890817 2316 90-91 -9.767406136722057 2316 92-93 -9.13459674816886 2316 94-95 -8.50201712869664 2316 96-97 -7.894249748329486 2316 98-99 -7.066014549877895 2316 100-101 -6.135237610819189 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 20340.0 3 1351.0 4 2337.0 5 1356.0 6 1192.0 7 1463.0 8 1889.0 9 2284.0 10 2321.0 11 2132.0 12 2044.0 13 1877.0 14 1773.0 15 1912.0 16 2153.0 17 2272.0 18 2364.0 19 2388.0 20 2644.0 21 2771.0 22 2808.0 23 2981.0 24 3362.0 25 3320.0 26 3609.0 27 3698.0 28 3963.0 29 4157.0 30 4425.0 31 4256.0 32 4136.0 33 3990.0 34 3704.0 35 3135.0 36 2009.0 37 892.0 38 106.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.69157477021636 20.581064400624317 14.44295959276526 23.284401236394054 2 15.80773097360775 20.875175377545048 40.062307360885555 23.254786287961643 3 19.003465294550036 26.28898456368791 30.238370261472227 24.469179880289822 4 13.170060108127469 16.853781663103458 38.29346645921714 31.68269176955193 5 13.485174331145606 37.34664542081276 35.377689624817535 13.790490623224095 6 30.575897765699228 37.71134576686098 17.827728564612162 13.885027902827623 7 26.636007005971464 32.69350923374748 23.147360981655414 17.52312277862564 8 25.374378516776325 35.476892236545694 21.002907697956672 18.145821548721308 9 26.296662165468867 16.22425027868154 21.473432359684978 36.00565519616462 10-11 24.909974117558797 26.237386998762148 28.854701976818337 19.997936906860723 12-13 25.50069981718131 24.409714465471886 28.569409554898034 21.520176162448767 14-15 22.837339137017413 25.522331566994698 26.487982588947766 25.152346707040117 16-17 22.060962920223496 28.44180117270526 27.477697394700634 22.01953851237061 18-19 22.24608149670759 27.135985963265785 29.35931897356381 21.258613566462813 20-21 22.49150727325829 26.704822024215158 29.45067557527656 21.352995127249997 22-23 22.624321145231125 26.32830292564507 29.31201041119203 21.735365517931776 24-25 22.38600102933608 26.607822954194543 29.299536798764798 21.70663921770458 26-27 22.27798472853798 26.74761978772367 29.14011541334724 21.834280070391113 28-29 22.35960741679651 27.052970676499616 29.02390268585829 21.56351922084559 30-31 22.423170238576628 26.891427416093812 28.894561261625558 21.790841083704006 32-33 22.500447681012357 26.983724308084124 28.74410553333731 21.771722477566207 34-35 22.672139016379298 26.78017657496346 28.847408813647075 21.700275595010165 36-37 23.151118048289625 26.679359416362214 28.438048518321583 21.73147401702658 38-39 22.882548799032215 26.54396011283275 28.627069683830886 21.94642140430415 40-41 22.54447084084036 26.400293372024425 29.037452765509652 22.017783021625554 42-43 22.87653193432595 26.538306323145218 28.507253456808478 22.077908285720355 44-45 22.732797891556 26.90197325820487 28.331080236745542 22.03414861349359 46-47 22.129757897948057 26.618723025450468 28.730677095575547 22.52084198102592 48-49 22.56001508697196 26.976837060702874 27.982450301739437 22.48069755058573 50-51 22.6030579957073 27.223242537561127 27.81957382780519 22.354125638926384 52-53 21.87174088431492 27.094197052283597 28.276333654735435 22.75772840866605 54-55 21.46632728072851 26.77615030329701 28.89997949682201 22.85754291915247 56-57 21.615674873806515 26.928744237910813 28.50280264035603 22.952778247926645 58-59 21.723138541425566 26.600315987561764 28.54051986998219 23.136025601030482 60-61 21.463748768793092 26.719064832730854 28.537501187458442 23.279685211017615 62-63 21.79258437410978 26.616389542758046 28.312117986316228 23.278908096815947 64-65 21.534628446672595 26.667744804722243 28.255640009703242 23.541986738901915 66-67 21.616480673053523 26.963362486404343 27.917417842474084 23.50273899806805 68-69 21.76180889890709 26.731361041803677 27.884011988373086 23.622818070916146 70-71 21.812513916722335 26.80620500259779 27.63601276627329 23.74526831440659 72-73 22.183228394060485 27.054671640837352 27.415937989982847 23.346161975119315 74-75 22.532312464610158 27.21238829119378 26.9184371845688 23.33686205962727 76-77 22.546964642438912 27.22915990119958 27.20701837254844 23.016857083813065 78-79 22.502248890382656 27.233815403169203 26.94714366208321 23.316792044364924 80-81 22.876863256604462 27.138699907609155 27.004807288501535 22.979629547284848 82-83 22.833642205003237 27.004625778867343 27.105799491212967 23.055932524916457 84-85 22.98572324384233 27.028130605340294 26.89931411294047 23.08683203787691 86-87 23.024023205559992 27.06163490178519 26.899565869137774 23.014776023517044 88-89 23.409458037553538 26.881258305299088 26.55867925367162 23.150604403475754 90-91 23.386219445273465 26.66287991836027 26.78851652530543 23.162384111060835 92-93 23.83402628293968 26.4775553131051 26.572405862058794 23.11601254189642 94-95 23.466868625271708 26.20117831043267 26.707222315114436 23.624730749181182 96-97 23.73086378639921 26.077651645004263 26.3900996111541 23.80138495744243 98-99 24.102772249145442 25.984517656033763 26.120464959338065 23.792245135482734 100-101 24.318356515821375 26.129870892880746 25.975157773110276 23.576614818187604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1270.0 1 1343.0 2 991.5 3 614.0 4 810.0 5 987.5 6 1069.0 7 1054.5 8 960.0 9 864.0 10 758.5 11 693.0 12 591.0 13 495.0 14 436.5 15 377.0 16 336.0 17 299.0 18 291.0 19 326.0 20 350.5 21 361.5 22 413.0 23 483.0 24 562.5 25 661.0 26 771.5 27 929.5 28 1069.0 29 1217.5 30 1463.5 31 1676.5 32 1861.0 33 2073.0 34 2182.5 35 2372.5 36 2657.5 37 2843.5 38 2948.0 39 3056.5 40 3217.0 41 3262.5 42 3366.5 43 3558.5 44 3676.0 45 3764.5 46 3800.0 47 3833.0 48 3887.0 49 3815.5 50 3625.0 51 3486.0 52 3371.0 53 3193.0 54 2992.5 55 2779.5 56 2453.5 57 2107.5 58 1795.0 59 1532.5 60 1309.5 61 1058.5 62 843.5 63 669.5 64 528.0 65 417.5 66 294.0 67 210.5 68 178.5 69 137.5 70 98.0 71 71.5 72 57.5 73 45.0 74 28.0 75 17.0 76 15.5 77 11.0 78 6.0 79 4.0 80 4.5 81 4.0 82 2.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.568632291740142 2 20.188509557734356 3 16.034774634972138 4 21.228927135501163 5 20.87271637158778 6 14.997883896451999 7 13.917789377160188 8 11.152747407773154 9 2.7112576708753613 10-11 5.977992523100797 12-13 3.3015623897862736 14-15 6.8209071030542425 16-17 10.60388305001058 18-19 13.567309727022641 20-21 13.95967059321436 22-23 11.923802638075756 24-25 14.341891796571913 26-27 12.06840304718911 28-29 12.004919940749101 30-31 12.781265429921703 32-33 11.372293150878184 34-35 18.258005925089936 36-37 22.89315440502222 38-39 22.01320801297877 40-41 17.050063483106438 42-43 16.976440713832265 44-45 22.385730408407987 46-47 11.847975594272413 48-49 20.519150737109403 50-51 20.30577696268604 52-53 10.384778161811385 54-55 11.842685335402413 56-57 11.440184806376525 58-59 12.383614304859984 60-61 11.825932848980743 62-63 11.788901036890739 64-65 12.767158072935036 66-67 11.233423855540664 68-69 12.184788742329125 70-71 10.90542780560062 72-73 10.30762855329054 74-75 10.464572899767228 76-77 10.401530648233054 78-79 10.805353741976441 80-81 10.770526204415603 82-83 9.365962474430416 84-85 9.307769626860408 86-87 9.41842420822459 88-89 10.757300557240601 90-91 8.415920152359456 92-93 10.29572547083304 94-95 10.35568173802638 96-97 9.98007335825633 98-99 9.849139451223813 100-101 9.955826338435495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 113416.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.63773753031496 #Duplication Level Percentage of deduplicated Percentage of total 1 97.88873919148259 92.63968806776985 2 1.553208199357544 2.939842198014647 3 0.2840708806171807 0.8065147631956257 4 0.08899537624829973 0.3368928423519286 5 0.045996658384613025 0.21765098417372847 6 0.029997778081433436 0.17033531091379997 7 0.027997923480460343 0.18547620936163536 8 0.00699948082330195 0.052993202320329835 9 0.011999109977297263 0.10220117585659576 >10 0.04699651407616978 0.8583006157297253 >50 0.008999332482668786 0.5677843102952308 >100 0.005999554988445856 1.1223203200169063 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 284 0.2504055865133667 No Hit NNNNNNNNGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 228 0.2010298370600268 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 211 0.18604077026169147 No Hit NNNNNNNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 187 0.16487973478168863 No Hit NNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 166 0.14636382873668619 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.817098116667842E-4 0.0 0.0 0.0 6 0.0 8.817098116667842E-4 0.0 0.0 0.0 7 0.0 8.817098116667842E-4 0.0 0.0 0.0 8 0.0 8.817098116667842E-4 0.0 0.0 0.0 9 0.0 8.817098116667842E-4 0.0 0.0 0.0 10-11 0.0 8.817098116667842E-4 0.0 0.0 0.0 12-13 0.0 8.817098116667842E-4 0.0 0.0 0.0 14-15 0.0 8.817098116667842E-4 0.0 0.0 0.0 16-17 0.0 8.817098116667842E-4 0.0 0.0 0.0 18-19 0.0 8.817098116667842E-4 0.0 0.0 0.0 20-21 0.0 8.817098116667842E-4 0.0 4.408549058333921E-4 0.0 22-23 0.0 8.817098116667842E-4 0.0 0.0013225647175001763 0.0 24-25 0.0 8.817098116667842E-4 0.0 0.003967694152500529 0.0 26-27 0.0 8.817098116667842E-4 0.0 0.005731113775834098 0.0 28-29 0.0 8.817098116667842E-4 0.0 0.014107356986668547 0.0 30-31 0.0 8.817098116667842E-4 0.0 0.058633702475841154 0.0 32-33 0.0 8.817098116667842E-4 0.0 0.15341750723002046 0.0 34-35 0.0 8.817098116667842E-4 0.0 0.27994286520420397 0.0 36-37 0.0 8.817098116667842E-4 0.0 0.47744586301756364 0.0 38-39 0.0 8.817098116667842E-4 0.0 0.7335825633067645 0.0 40-41 0.0 8.817098116667842E-4 0.0 1.0108802990759682 0.0 42-43 0.0 8.817098116667842E-4 0.0 1.2753932425760035 0.0 44-45 0.0 8.817098116667842E-4 0.0 1.6170557945968822 0.0 46-47 0.0 8.817098116667842E-4 0.0 1.9763525428510968 0.0 48-49 0.0 8.817098116667842E-4 0.0 2.3497566480919803 0.0 50-51 0.0 8.817098116667842E-4 0.0 2.743440079001199 0.0 52-53 0.0 8.817098116667842E-4 0.0 3.2521866403329334 0.0 54-55 0.0 8.817098116667842E-4 0.0 3.7512343937363335 0.0 56-57 0.0 8.817098116667842E-4 0.0 4.3225823516964095 0.0 58-59 0.0 0.0017634196233335684 0.0 4.914209635324822 0.0 60-61 0.0 0.0017634196233335684 0.0 5.488202722719898 0.0 62-63 0.0 0.0017634196233335684 0.0 6.020755448966636 0.0 64-65 0.0 0.0017634196233335684 0.0 6.499083021795867 0.0 66-67 0.0 0.0017634196233335684 0.0 7.010915567468435 0.0 68-69 0.0 0.0017634196233335684 0.0 7.570801297876843 0.0 70-71 0.0 0.0017634196233335684 0.0 8.119224800733582 0.0 72-73 0.0 0.0017634196233335684 0.0 8.668970868307824 0.0 74-75 0.0 0.0017634196233335684 0.0 9.181244268886225 0.0 76-77 0.0 0.0017634196233335684 0.0 9.71511955985046 0.0 78-79 0.0 0.0017634196233335684 0.0 10.287790082528037 0.0 80-81 0.0 0.0017634196233335684 0.0 10.863546589546448 0.0 82-83 0.0 0.0017634196233335684 0.0 11.394776751075685 0.0 84-85 0.0 0.0017634196233335684 0.0 11.900437328066587 0.0 86-87 0.0 0.0026451294350003525 0.0 12.414915003174155 0.0 88-89 0.0 0.0026451294350003525 4.408549058333921E-4 12.91087677223672 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 175 0.0 63.05232 1 TACTGTA 25 0.0060448986 54.350628 7 GGTATCA 80 2.561137E-9 50.155254 1 TATCAAC 210 0.0 50.15525 2 GCCTAAA 30 0.008302363 50.15525 1 AGGCCCG 30 0.009697067 48.224052 5 TAGACTG 40 4.9760897E-4 48.22405 5 ATCAACG 235 0.0 42.68532 3 TATAGTA 50 0.001230414 40.1242 2 TGGTATC 50 0.001230414 40.1242 2 CAACGCA 255 0.0 37.82279 5 TCAACGC 260 0.0 36.630653 4 AACGCAG 265 0.0 35.873123 6 GGAGCCG 55 0.0023834272 35.072037 5 GTAGAAC 60 0.003011488 33.436836 3 GTGGTAT 60 0.003011488 33.436836 1 TAGGACC 60 0.003020073 33.41744 4 GTACATG 220 0.0 31.916977 1 ATAGTAT 65 0.004456611 30.86477 3 TACATGG 235 0.0 29.879725 2 >>END_MODULE