FastQCFastQC Report
Sat 18 Jun 2016
SRR1930943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113416
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11711.0324821894618041No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT8500.7494533399167667TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG4460.39324257600338575TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT4330.3817803484517176RNA PCR Primer, Index 10 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.28302884954503776No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT2760.24335190802003245TruSeq Adapter, Index 10 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2710.23894335896169852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.1736968328983565No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG1450.1278479226916837TruSeq Adapter, Index 10 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1300.058.417291
GTATCAA2900.045.827361
CAACGCA3250.040.8740775
ATCAACG3400.039.070813
AACGCAG3400.039.070816
TCAACGC3400.039.070814
ATATAGT756.9030484E-637.9712371
TATCAAC3600.036.9002082
ATGGGGA801.0778898E-535.582345
TATTCAG600.00395923631.6287485
GTATAGC600.00395923631.6287486
ACGCAGA4350.030.5381037
ATACACT804.4998896E-429.651954
CGCAGAG4500.029.5201648
GCAGAGT4500.028.4658729
CGCAAGT350.00825587527.2243976-77
TAACAGA700.00840927527.1103554
TAGGAGA700.00840927527.1103554
TATACAT700.00840927527.1103555
TATAGTA908.960166E-426.3572922
Produced by FastQC (version 0.11.5)