FastQCFastQC Report
Sat 18 Jun 2016
SRR1930942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019840
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35820.1773407794676806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.053.056161
GTATCAA34250.046.361081
ATCAACG43050.036.9900363
TCAACGC43600.036.8557474
TATCAAC44100.035.3189162
CAACGCA44400.035.1240355
AACGCAG47950.032.5935176
ACGCAGA54350.028.1086447
GTACATG37300.028.0208641
CGCAGAG56350.026.7990958
TACATGG40550.025.4007912
ACATGGG42350.023.604693
GCAGAGT63900.022.5556269
CATGGGG30050.022.3924754
AGAGTAC56650.020.3817810-11
GTGGTAT8500.020.0995831
GAGTACT42250.019.71613112-13
AGTACTT46250.018.90405712-13
CAGAGTA60200.018.73810210-11
GAGTACA30000.017.7546311
Produced by FastQC (version 0.11.5)