##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930942_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019840 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.040885911755385 30.0 10.0 31.0 2.0 33.0 2 21.971795785804815 30.0 10.0 31.0 2.0 33.0 3 22.411456848067175 30.0 10.0 31.0 2.0 33.0 4 24.398766733998734 33.0 10.0 35.0 2.0 37.0 5 24.384536894011408 32.0 10.0 35.0 2.0 37.0 6 25.128903279467682 32.0 17.0 35.0 2.0 37.0 7 25.2146620524398 32.0 17.0 35.0 2.0 37.0 8 25.97602186311787 33.0 17.0 35.0 2.0 37.0 9 27.140060598859314 34.0 17.0 37.0 2.0 39.0 10-11 27.38714700174271 34.0 18.5 37.0 2.0 39.0 12-13 27.313551815985427 34.0 18.5 37.0 2.0 39.0 14-15 27.545542221166034 34.0 17.0 38.0 2.0 40.0 16-17 26.672845373891 33.0 14.0 38.0 2.0 40.0 18-19 26.073439727899242 33.0 10.5 38.0 2.0 40.0 20-21 25.57914661557351 32.0 10.0 38.0 2.0 40.0 22-23 25.526429568678708 32.0 10.0 38.0 2.0 40.0 24-25 25.231510416666666 32.0 10.0 38.0 2.0 40.0 26-27 24.88482676845691 32.0 9.0 38.0 2.0 40.0 28-29 24.793393783666033 32.0 8.5 38.0 2.0 40.0 30-31 24.699061559331433 31.0 8.0 38.0 2.0 40.0 32-33 24.401923419676805 31.0 5.0 37.0 2.0 39.5 34-35 21.470775407953106 26.0 2.0 36.0 2.0 39.0 36-37 19.846549726711025 19.0 2.0 35.0 2.0 38.5 38-39 18.911774447480987 19.0 2.0 34.0 2.0 38.0 40-41 20.249289795231306 24.0 2.0 35.0 2.0 38.0 42-43 20.175415626980353 24.0 2.0 35.0 2.0 38.0 44-45 18.20419216373574 19.0 2.0 34.0 2.0 38.0 46-47 19.061982632287705 19.0 2.0 33.0 2.0 38.0 48-49 18.437663626821926 18.5 2.0 33.5 2.0 38.0 50-51 16.463940460630546 11.0 2.0 31.5 2.0 37.5 52-53 19.43163146585868 23.0 2.0 33.5 2.0 38.0 54-55 21.070550637674273 27.0 2.0 35.5 2.0 38.5 56-57 21.28485226552598 28.0 2.0 36.0 2.0 39.0 58-59 20.92300603018061 27.5 2.0 36.0 2.0 39.0 60-61 20.593320015446768 26.5 2.0 35.5 2.0 39.0 62-63 20.11963002019962 26.0 2.0 35.0 2.0 38.5 64-65 19.61728255703422 25.5 2.0 34.0 2.0 38.0 66-67 19.184750277249684 24.0 2.0 34.0 2.0 38.0 68-69 18.73111731622307 22.5 2.0 34.0 2.0 37.5 70-71 18.130824471245248 21.5 2.0 33.5 2.0 36.5 72-73 17.648893476711024 20.0 2.0 33.0 2.0 36.0 74-75 17.112429202313052 18.0 2.0 32.0 2.0 36.0 76-77 16.53302291270596 12.0 2.0 32.0 2.0 35.0 78-79 16.025604750871356 7.5 2.0 31.0 2.0 35.0 80-81 15.507019367870722 6.5 2.0 31.0 2.0 34.5 82-83 15.030383594740178 2.0 2.0 31.0 2.0 34.0 84-85 14.506700530735108 2.0 2.0 30.0 2.0 34.0 86-87 13.943373732572876 2.0 2.0 30.0 2.0 34.0 88-89 13.376926637753485 2.0 2.0 29.0 2.0 34.0 90-91 12.789415250712928 2.0 2.0 29.0 2.0 34.0 92-93 12.156988919914447 2.0 2.0 29.0 2.0 34.0 94-95 11.484854988513941 2.0 2.0 26.5 2.0 33.5 96-97 10.74311777170469 2.0 2.0 25.5 2.0 33.0 98-99 9.930959135377059 2.0 2.0 24.0 2.0 32.5 100-101 8.858869514416984 2.0 2.0 10.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.823639651186934 1101 2 7.964515001292337 1101 3 7.646035195739493 1101 4 9.386557409853076 1101 5 9.181570202039119 1101 6 8.686455367293323 1101 7 8.381036518434087 1101 8 7.371173386384438 1101 9 7.62266260012014 1101 10-11 7.0518398903546675 1101 12-13 6.854392484784157 1101 14-15 7.521500601937973 1101 16-17 8.181556301317041 1101 18-19 8.460413631132393 1101 20-21 8.97712303504668 1101 22-23 8.809537418140195 1101 24-25 8.925411647321791 1101 26-27 9.000070630886743 1101 28-29 9.074774229823209 1101 30-31 8.651307889560883 1101 32-33 8.597855785327607 1101 34-35 11.06648495834666 1101 36-37 5.588025573861145 1101 38-39 8.59649822706939 1101 40-41 10.047969997089709 1101 42-43 10.1618395805339 1101 44-45 4.7688826217243765 1101 46-47 6.290675172728399 1101 48-49 9.456070930987018 1101 50-51 -3.2138913813666043 1101 52-53 6.030767176176752 1101 54-55 8.482570745746226 1101 56-57 8.895661635250129 1101 58-59 9.069787614158777 1101 60-61 8.947471468832262 1101 62-63 8.97543945389885 1101 64-65 8.958270866500985 1101 66-67 9.001312343844091 1101 68-69 9.121538247522544 1101 70-71 9.190828895565378 1101 72-73 9.013567973671531 1101 74-75 8.960419839309417 1101 76-77 8.956867835929014 1101 78-79 8.92977162887044 1101 80-81 8.699669436212831 1101 82-83 8.397725069869182 1101 84-85 8.206502671037772 1101 86-87 7.983704582627579 1101 88-89 7.995863796880606 1101 90-91 7.8212659776612465 1101 92-93 7.740788366157673 1101 94-95 7.513429934162337 1101 96-97 7.263563088734859 1101 98-99 7.309527593769628 1101 100-101 6.568809752126635 1102 1 6.55600295096772 1102 2 7.63460131810163 1102 3 7.376011625622073 1102 4 9.285975977971788 1102 5 9.298345672717296 1102 6 8.48973334717071 1102 7 8.343766855167239 1102 8 7.369040310811666 1102 9 7.348783762734346 1102 10-11 6.947087006702983 1102 12-13 6.473544686956842 1102 14-15 7.012759961021516 1102 16-17 7.777672601953906 1102 18-19 8.26693512031461 1102 20-21 8.608476304715296 1102 22-23 8.29853156816985 1102 24-25 8.320137025818479 1102 26-27 8.376877327848295 1102 28-29 8.195096582039813 1102 30-31 8.204852141799016 1102 32-33 8.129372294436788 1102 34-35 10.57754306008989 1102 36-37 1.2552266792368165 1102 38-39 3.784999115906583 1102 40-41 8.401345506182409 1102 42-43 9.604197519215944 1102 44-45 1.1079871578021638 1102 46-47 1.9285878781759038 1102 48-49 7.525378395570566 1102 50-51 -4.583130372731359 1102 52-53 4.857712758127807 1102 54-55 7.559944814517134 1102 56-57 8.200364339374076 1102 58-59 8.583451285651321 1102 60-61 8.34367649721311 1102 62-63 8.21078667528549 1102 64-65 8.261219181141772 1102 66-67 8.248886702907583 1102 68-69 8.26502295279358 1102 70-71 8.285045860969582 1102 72-73 8.180716604452257 1102 74-75 7.65271661333621 1102 76-77 7.717222488431325 1102 78-79 7.7924723525519255 1102 80-81 7.7952677869730795 1102 82-83 7.566829632685618 1102 84-85 7.371575602925969 1102 86-87 7.386271737853894 1102 88-89 7.361866075013339 1102 90-91 7.088627938281457 1102 92-93 7.038871361135705 1102 94-95 7.163520756433668 1102 96-97 7.120465313969632 1102 98-99 6.611543063859555 1102 100-101 5.745555997463526 1103 1 6.723929213234328 1103 2 7.713827391324688 1103 3 7.485046555788724 1103 4 9.122243468119784 1103 5 8.985908704601087 1103 6 8.265303752125813 1103 7 7.977485054574068 1103 8 7.253623049339009 1103 9 7.410526434033532 1103 10-11 6.664284265538637 1103 12-13 6.653497492982197 1103 14-15 7.0043679588118835 1103 16-17 7.711424213606637 1103 18-19 8.164527902918476 1103 20-21 8.656677144123933 1103 22-23 8.492567537121694 1103 24-25 8.593214785087522 1103 26-27 8.524973433138765 1103 28-29 8.348732692187465 1103 30-31 8.318795359409265 1103 32-33 8.348698898372685 1103 34-35 10.62997516664753 1103 36-37 -2.8238101749311255 1103 38-39 -1.4455878317300872 1103 40-41 7.076271557181826 1103 42-43 9.352759399550962 1103 44-45 1.2365656192110208 1103 46-47 1.26269827109558 1103 48-49 7.978173012930149 1103 50-51 -4.803110522829787 1103 52-53 4.982909189445461 1103 54-55 7.9720644650202175 1103 56-57 8.661432710745373 1103 58-59 9.052870744039474 1103 60-61 8.896014268473706 1103 62-63 8.935576389842236 1103 64-65 8.686868048931732 1103 66-67 8.45939877025684 1103 68-69 8.254614917532361 1103 70-71 8.421462269256256 1103 72-73 8.438998420841106 1103 74-75 8.320679512799572 1103 76-77 7.993855591476844 1103 78-79 7.956610394489012 1103 80-81 7.953325589939114 1103 82-83 7.532057751887699 1103 84-85 7.440171185094318 1103 86-87 7.225419964712293 1103 88-89 7.16484702652302 1103 90-91 6.948553404621416 1103 92-93 6.936900389642297 1103 94-95 6.469032307132641 1103 96-97 6.363683428803775 1103 98-99 6.139880728938097 1103 100-101 5.540961714928434 1104 1 6.5717462349352544 1104 2 7.731222969293011 1104 3 7.241137436271099 1104 4 9.01203586081629 1104 5 8.825723695684765 1104 6 8.219366216917013 1104 7 7.989156571152478 1104 8 6.937327863896254 1104 9 7.3613856260084525 1104 10-11 6.61733012666669 1104 12-13 6.250418349415739 1104 14-15 6.903355110754632 1104 16-17 7.538886498905772 1104 18-19 8.003937871332536 1104 20-21 8.373888114637246 1104 22-23 8.227317510897208 1104 24-25 8.194009743251367 1104 26-27 8.338685668255241 1104 28-29 7.988521523533567 1104 30-31 7.873443957726494 1104 32-33 7.986908432690502 1104 34-35 10.511339785472959 1104 36-37 -3.1050338290320525 1104 38-39 -1.7629805606180788 1104 40-41 6.387860903693451 1104 42-43 8.636029535102434 1104 44-45 0.5781237294419377 1104 46-47 0.2642396883058389 1104 48-49 7.101669313658348 1104 50-51 -5.319355784614942 1104 52-53 3.833089652783624 1104 54-55 6.668084105543983 1104 56-57 7.147033523510288 1104 58-59 7.333398639773495 1104 60-61 6.980551338246389 1104 62-63 7.075190067499879 1104 64-65 7.421520571209598 1104 66-67 7.006157358615241 1104 68-69 7.217400748278859 1104 70-71 7.031947661891902 1104 72-73 6.83409657813365 1104 74-75 6.7358504122930825 1104 76-77 6.626192782723955 1104 78-79 6.3929279452123495 1104 80-81 6.318611359171376 1104 82-83 6.217932076364278 1104 84-85 6.120791222676274 1104 86-87 5.858445791625483 1104 88-89 5.683897984914793 1104 90-91 5.583914182368547 1104 92-93 5.508570300375432 1104 94-95 5.387817977294608 1104 96-97 5.07807866617749 1104 98-99 4.558247778547061 1104 100-101 4.058491652935036 1105 1 5.9535973478112965 1105 2 6.989059592503594 1105 3 6.700088549792849 1105 4 8.437425060720727 1105 5 8.509870297005882 1105 6 7.520574334745628 1105 7 7.462725200122769 1105 8 6.5205388357498855 1105 9 6.307813251937464 1105 10-11 5.901119006300444 1105 12-13 5.723420370478706 1105 14-15 6.024979838750546 1105 16-17 6.701689254783322 1105 18-19 7.645275065993832 1105 20-21 7.620622128545776 1105 22-23 7.3951283703978135 1105 24-25 7.563877502454307 1105 26-27 7.489749470565577 1105 28-29 7.474687896403541 1105 30-31 7.184464239383896 1105 32-33 6.873600411072228 1105 34-35 9.256863098724804 1105 36-37 -3.6649270290914586 1105 38-39 -2.383424936187108 1105 40-41 5.229045711498756 1105 42-43 7.452765938126927 1105 44-45 -3.2960098931681383 1105 46-47 -4.14977635105646 1105 48-49 5.000575808825232 1105 50-51 -9.67034048781571 1105 52-53 0.7284663905426321 1105 54-55 5.457283687125834 1105 56-57 6.3170982233533834 1105 58-59 6.684455662160154 1105 60-61 6.671549859577318 1105 62-63 6.7608188892782835 1105 64-65 6.568531593428379 1105 66-67 6.442359568224653 1105 68-69 6.554319046970011 1105 70-71 6.507430812167296 1105 72-73 6.391760151863707 1105 74-75 6.120188465744711 1105 76-77 6.1830182287460715 1105 78-79 5.878968728067537 1105 80-81 5.8150929016363 1105 82-83 5.737326209470325 1105 84-85 5.525198173066606 1105 86-87 5.375731691321354 1105 88-89 5.547184698201505 1105 90-91 5.151525841254442 1105 92-93 4.878520387223023 1105 94-95 4.588803326202397 1105 96-97 4.291176833015269 1105 98-99 4.063442814351514 1105 100-101 3.709205159188709 1106 1 5.738139518496656 1106 2 6.941517911705564 1106 3 6.577032083549316 1106 4 7.854936110102447 1106 5 7.850522869991018 1106 6 7.07472949444351 1106 7 6.912345941198478 1106 8 6.218818846731978 1106 9 6.049147360477111 1106 10-11 5.638824180264276 1106 12-13 5.475868461263978 1106 14-15 5.9599125734523355 1106 16-17 6.415202608250485 1106 18-19 6.8282129902712505 1106 20-21 7.155615695410006 1106 22-23 6.9050246327190195 1106 24-25 6.911525774121056 1106 26-27 7.057503041090509 1106 28-29 7.136224742928839 1106 30-31 6.7083529757437645 1106 32-33 6.793791843965742 1106 34-35 8.867969005904616 1106 36-37 -0.18109169583349427 1106 38-39 0.8142908968636 1106 40-41 6.507157976447047 1106 42-43 7.642240897347847 1106 44-45 -3.7230753604183633 1106 46-47 -4.3381261844409345 1106 48-49 4.548411960356532 1106 50-51 -9.614381896753194 1106 52-53 0.4034422999296261 1106 54-55 4.795211194568356 1106 56-57 5.856464707523564 1106 58-59 6.20464063500102 1106 60-61 6.1912121064912995 1106 62-63 6.239485103741266 1106 64-65 5.979044790777603 1106 66-67 5.981779347829189 1106 68-69 5.7305578966499695 1106 70-71 5.585855312993644 1106 72-73 5.573834582436984 1106 74-75 5.4171086356440945 1106 76-77 5.201138138090002 1106 78-79 4.883240437881973 1106 80-81 4.798726896586691 1106 82-83 4.683237265312094 1106 84-85 4.479351717751328 1106 86-87 4.569983859476528 1106 88-89 4.314498446808557 1106 90-91 3.947920055200621 1106 92-93 3.7586207333741797 1106 94-95 3.536363310482935 1106 96-97 3.349234402851131 1106 98-99 3.0382685275144095 1106 100-101 2.838724369770274 1107 1 5.733982474616749 1107 2 6.8948666414977104 1107 3 6.392736471540079 1107 4 7.558400313335703 1107 5 7.913340421954064 1107 6 6.525999702295707 1107 7 6.5141935162562135 1107 8 6.221590209318581 1107 9 6.2029579840336915 1107 10-11 5.446214480495225 1107 12-13 5.320441209531879 1107 14-15 5.607256684306833 1107 16-17 6.62813563365464 1107 18-19 6.903039780109591 1107 20-21 7.308964425202163 1107 22-23 7.1613756719799895 1107 24-25 7.149632009686872 1107 26-27 7.002768630005061 1107 28-29 7.135993796046623 1107 30-31 7.122209788676791 1107 32-33 6.731667132649346 1107 34-35 8.882518659484287 1107 36-37 -0.4660801484893824 1107 38-39 0.9073624903970874 1107 40-41 6.6080817639759175 1107 42-43 7.790739742613432 1107 44-45 -3.3420130047601653 1107 46-47 -4.301174683286202 1107 48-49 4.668273392227199 1107 50-51 -9.658261804374442 1107 52-53 0.2734192052414066 1107 54-55 4.815765467085676 1107 56-57 5.8481506197637465 1107 58-59 6.080391212368227 1107 60-61 6.346870305105618 1107 62-63 6.341563625681218 1107 64-65 6.284356569068596 1107 66-67 6.227968724272607 1107 68-69 5.961504778867059 1107 70-71 5.838973095903576 1107 72-73 5.907783774122894 1107 74-75 5.947824570978966 1107 76-77 5.632546914071526 1107 78-79 5.615111107627932 1107 80-81 5.431752300743733 1107 82-83 4.964761514734725 1107 84-85 5.2199983690215355 1107 86-87 5.094002335227101 1107 88-89 4.893482280526804 1107 90-91 5.032215667209856 1107 92-93 4.7110456756374575 1107 94-95 4.67331481163767 1107 96-97 4.500966504467758 1107 98-99 4.012864370470529 1107 100-101 3.446576563765655 1108 1 6.3250417355646995 1108 2 6.5843035474042 1108 3 6.745010532912257 1108 4 8.225986141985672 1108 5 8.388730206767079 1108 6 7.542896599986346 1108 7 7.634671136875575 1108 8 6.63047544182383 1108 9 5.2127383697381156 1108 10-11 6.1504198723395085 1108 12-13 5.488074505089919 1108 14-15 6.1204922424174555 1108 16-17 6.318324741375886 1108 18-19 6.88917327555728 1108 20-21 7.908878412081023 1108 22-23 7.752009162471744 1108 24-25 7.3881596104274045 1108 26-27 7.721152640595353 1108 28-29 7.73489511884252 1108 30-31 7.546492911712999 1108 32-33 7.528767466239284 1108 34-35 9.772930674449032 1108 36-37 -3.362737420893268 1108 38-39 -1.8263107104033551 1108 40-41 5.833828101259687 1108 42-43 8.099970569451727 1108 44-45 -2.9019077625853207 1108 46-47 -4.021848008535844 1108 48-49 5.440796770505422 1108 50-51 -9.636225485031181 1108 52-53 0.9964803031917384 1108 54-55 5.867836224716999 1108 56-57 7.084743310740961 1108 58-59 7.56372074614627 1108 60-61 7.590736776618314 1108 62-63 7.7116774251153615 1108 64-65 7.4254574048606266 1108 66-67 7.263329918808736 1108 68-69 7.261025263976656 1108 70-71 7.290409493000055 1108 72-73 7.119390699665928 1108 74-75 7.093628044763687 1108 76-77 6.860676288378382 1108 78-79 6.782690328355663 1108 80-81 6.853689831649538 1108 82-83 6.747791709635393 1108 84-85 6.5192459469427035 1108 86-87 6.39450409678904 1108 88-89 6.257455447957234 1108 90-91 6.163560015949562 1108 92-93 6.382334694041624 1108 94-95 6.0411058162731255 1108 96-97 6.123905906516875 1108 98-99 5.775653398235919 1108 100-101 5.130768431395758 1109 1 4.958818397947603 1109 2 6.416778107510247 1109 3 6.292408753130001 1109 4 7.912186363448132 1109 5 7.698129025095117 1109 6 5.290073761872172 1109 7 5.098671363187577 1109 8 3.3177304388736957 1109 9 3.05355818497053 1109 10-11 2.7069978731962614 1109 12-13 3.5781860849901825 1109 14-15 4.166664942723788 1109 16-17 3.006129353291218 1109 18-19 3.908521941528843 1109 20-21 4.597988359301837 1109 22-23 6.008583324107008 1109 24-25 5.932819859532255 1109 26-27 4.6054294120929775 1109 28-29 4.608650402823674 1109 30-31 4.725033886309475 1109 32-33 4.4143606865782665 1109 34-35 6.773013520807428 1109 36-37 -4.71470512293735 1109 38-39 -3.35912291993594 1109 40-41 3.559086339420741 1109 42-43 4.1102758481792065 1109 44-45 -4.50216499042493 1109 46-47 -5.867412293268675 1109 48-49 2.066503995516129 1109 50-51 -9.974828655015743 1109 52-53 -1.238395184793518 1109 54-55 2.4852527519593046 1109 56-57 3.8537555396717167 1109 58-59 4.586004395912283 1109 60-61 5.01651351174436 1109 62-63 4.947293478718976 1109 64-65 4.6308789917927236 1109 66-67 4.706396284803738 1109 68-69 4.922852490927184 1109 70-71 5.097777565225911 1109 72-73 5.009179784961308 1109 74-75 5.091693279198676 1109 76-77 5.017097285282759 1109 78-79 5.124855891751313 1109 80-81 4.638246866315685 1109 82-83 4.803478606978651 1109 84-85 4.687366466167102 1109 86-87 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2313 68-69 -16.250584095864166 2313 70-71 -15.60148862089007 2313 72-73 -15.09283982670179 2313 74-75 -14.566555633068354 2313 76-77 -14.07374871121052 2313 78-79 -13.581415619385774 2313 80-81 -13.028318332723211 2313 82-83 -12.551662148914588 2313 84-85 -12.041033105233637 2313 86-87 -11.501978768716691 2313 88-89 -10.94517289631126 2313 90-91 -10.36763191669642 2313 92-93 -9.746929402450903 2313 94-95 -9.212828375429305 2313 96-97 -8.463075906582297 2313 98-99 -7.661769521205649 2313 100-101 -6.6178019811805004 2314 1 -20.669712675498726 2314 2 -19.557327353883352 2314 3 -19.970773921069622 2314 4 -21.867003843708176 2314 5 -21.864488677353094 2314 6 -22.642129627958337 2314 7 -22.715829578432007 2314 8 -23.490649975438924 2314 9 -24.64276949864529 2314 10-11 -24.917295912114472 2314 12-13 -24.849073802015468 2314 14-15 -25.04355162978092 2314 16-17 -24.185490232396166 2314 18-19 -23.551694630975863 2314 20-21 -23.066324258400613 2314 22-23 -23.008601233331788 2314 24-25 -22.728197089620284 2314 26-27 -22.400926520110414 2314 28-29 -22.288948467232824 2314 30-31 -22.204580049660393 2314 32-33 -21.91567697820516 2314 34-35 -19.090922449060745 2314 36-37 -17.439752203916633 2314 38-39 -16.55568600844818 2314 40-41 -17.890301607848563 2314 42-43 -17.811569726208738 2314 44-45 -15.825846723004968 2314 46-47 -16.613645814925924 2314 48-49 -16.096160787911366 2314 50-51 -14.08782300529823 2314 52-53 -16.980391418315175 2314 54-55 -18.562063632221484 2314 56-57 -18.771479865224705 2314 58-59 -18.405521027816533 2314 60-61 -18.100011911984453 2314 62-63 -17.623563395104 2314 64-65 -17.11841479351801 2314 66-67 -16.715449289584207 2314 68-69 -16.26782547517451 2314 70-71 -15.67811697338049 2314 72-73 -15.198682738579183 2314 74-75 -14.688203142646898 2314 76-77 -14.13050158477374 2314 78-79 -13.633618684519874 2314 80-81 -13.131527144983748 2314 82-83 -12.667801995657882 2314 84-85 -12.162680614812182 2314 86-87 -11.621471105881442 2314 88-89 -11.066102015085207 2314 90-91 -10.488800499071898 2314 92-93 -9.850617141914505 2314 94-95 -9.212828375429305 2314 96-97 -8.474091232252796 2314 98-99 -7.686673735765035 2314 100-101 -6.645340295356746 2315 1 -20.669712675498726 2315 2 -19.557327353883352 2315 3 -19.970773921069622 2315 4 -16.640265352033346 2315 5 -21.864488677353094 2315 6 -22.642129627958337 2315 7 -22.715829578432007 2315 8 -23.490649975438924 2315 9 -19.801437471221195 2315 10-11 -18.040214619264326 2315 12-13 -14.599073802015468 2315 14-15 -14.466421365334302 2315 16-17 -19.881131760309977 2315 18-19 -23.551694630975863 2315 20-21 -23.066324258400613 2315 22-23 -23.008601233331788 2315 24-25 -22.728197089620284 2315 26-27 -22.400926520110414 2315 28-29 -22.288948467232824 2315 30-31 -22.204580049660393 2315 32-33 -21.91567697820516 2315 34-35 -19.090922449060745 2315 36-37 -17.439752203916633 2315 38-39 -16.55568600844818 2315 40-41 -17.890301607848563 2315 42-43 -17.811569726208738 2315 44-45 -15.825846723004968 2315 46-47 -16.613645814925924 2315 48-49 -16.096160787911366 2315 50-51 -14.08782300529823 2315 52-53 -16.980391418315175 2315 54-55 -18.562063632221484 2315 56-57 -18.771479865224705 2315 58-59 -18.405521027816533 2315 60-61 -18.100011911984453 2315 62-63 -17.623563395104 2315 64-65 -17.11841479351801 2315 66-67 -16.715449289584207 2315 68-69 -16.26782547517451 2315 70-71 -15.67811697338049 2315 72-73 -15.198682738579183 2315 74-75 -14.688203142646898 2315 76-77 -14.13050158477374 2315 78-79 -13.633618684519874 2315 80-81 -13.131527144983748 2315 82-83 -12.667801995657882 2315 84-85 -12.162680614812182 2315 86-87 -11.621471105881442 2315 88-89 -11.066102015085207 2315 90-91 -10.488800499071898 2315 92-93 -9.850617141914505 2315 94-95 -9.212828375429305 2315 96-97 -8.474091232252796 2315 98-99 -7.686673735765035 2315 100-101 -6.645340295356746 2316 1 -20.669712675498726 2316 2 -19.557327353883352 2316 3 -19.970773921069622 2316 4 -21.867003843708176 2316 5 -21.864488677353094 2316 6 -22.642129627958337 2316 7 -22.715829578432007 2316 8 -23.490649975438924 2316 9 -24.64276949864529 2316 10-11 -24.917295912114472 2316 12-13 -24.849073802015468 2316 14-15 -25.04355162978092 2316 16-17 -24.185490232396166 2316 18-19 -23.551694630975863 2316 20-21 -23.066324258400613 2316 22-23 -23.008601233331788 2316 24-25 -22.728197089620284 2316 26-27 -22.400926520110414 2316 28-29 -22.288948467232824 2316 30-31 -22.204580049660393 2316 32-33 -21.91567697820516 2316 34-35 -19.090922449060745 2316 36-37 -17.439752203916633 2316 38-39 -16.55568600844818 2316 40-41 -17.890301607848563 2316 42-43 -17.811569726208738 2316 44-45 -15.825846723004968 2316 46-47 -16.613645814925924 2316 48-49 -16.096160787911366 2316 50-51 -14.08782300529823 2316 52-53 -16.980391418315175 2316 54-55 -18.562063632221484 2316 56-57 -18.771479865224705 2316 58-59 -18.405521027816533 2316 60-61 -18.100011911984453 2316 62-63 -17.623563395104 2316 64-65 -17.11841479351801 2316 66-67 -16.715449289584207 2316 68-69 -16.26782547517451 2316 70-71 -15.67811697338049 2316 72-73 -15.198682738579183 2316 74-75 -14.688203142646898 2316 76-77 -14.13050158477374 2316 78-79 -13.633618684519874 2316 80-81 -13.131527144983748 2316 82-83 -12.667801995657882 2316 84-85 -12.162680614812182 2316 86-87 -11.621471105881442 2316 88-89 -11.066102015085207 2316 90-91 -10.488800499071898 2316 92-93 -9.850617141914505 2316 94-95 -9.212828375429305 2316 96-97 -8.474091232252796 2316 98-99 -7.686673735765035 2316 100-101 -6.645340295356746 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 321006.0 3 20418.0 4 42265.0 5 24723.0 6 22163.0 7 27536.0 8 34933.0 9 41299.0 10 42667.0 11 40153.0 12 36327.0 13 33917.0 14 32933.0 15 35127.0 16 38025.0 17 39981.0 18 41362.0 19 42837.0 20 45414.0 21 47716.0 22 50607.0 23 53943.0 24 57033.0 25 60694.0 26 64153.0 27 68358.0 28 72659.0 29 76772.0 30 80035.0 31 80172.0 32 77953.0 33 76592.0 34 71069.0 35 59432.0 36 41041.0 37 16664.0 38 1845.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.359588493186635 20.87788196918303 14.330110139536167 23.43241939809417 2 15.120098452619676 21.230720215896103 40.84846667541246 22.800714656071765 3 18.27845998694904 26.25698522868838 30.713353502995787 24.751201281366793 4 12.36711401969618 17.26125100772988 39.39115726909154 30.9804777034824 5 12.905511473192202 37.94109704513563 35.887796925002775 13.265594556669392 6 29.992589109508806 38.1652065727094 18.289186549233758 13.55301776854804 7 26.024873276986295 33.01859568057415 23.478384075550913 17.478146966888644 8 24.760713581883657 36.04502212587563 21.399282476664816 17.7949818155759 9 25.758237051691257 16.30773074452138 21.472471778346574 36.46156042544079 10-11 24.249331853608624 26.720232771537756 29.35411811539952 19.6763172594541 12-13 24.861468567722223 24.46889343734007 29.348802964904518 21.320835030033187 14-15 22.0386493381917 25.86548005249169 27.167700291102392 24.928170318214214 16-17 21.642533347286015 28.83808281214863 27.781333980500385 21.73804986006497 18-19 21.539053852170017 27.84125398970021 29.865159363398767 20.754532794731002 20-21 22.14293979472582 27.082624593559434 29.918538318232834 20.85589729348191 22-23 21.855515413883868 26.887453710310254 29.724925152344472 21.532105723461402 24-25 21.7480314823263 27.131946582741712 29.685720458133037 21.434301476798954 26-27 21.713767319578093 27.258889380154223 29.521491538119694 21.505851762147994 28-29 21.683782415512496 27.51923930843136 29.48932752218071 21.307650753875436 30-31 21.904173309601937 27.307823510352986 29.367173884807578 21.420829295237496 32-33 21.821130398222117 27.440619767233642 29.175421273652873 21.56282856089137 34-35 22.130191991513705 27.390582364824954 29.236309349380857 21.242916294280484 36-37 22.57359615044459 27.38657329403213 28.81634176036853 21.223488795154747 38-39 22.416030899862736 27.37948095891723 28.838924889073326 21.36556325214671 40-41 22.35765609808153 27.375530227826488 28.946754634981424 21.320059039110557 42-43 22.42748731123523 27.41010069753349 28.54439096177944 21.618021029451846 44-45 22.6694420499396 27.614583063050596 28.28879233989091 21.42718254711889 46-47 22.05443119773513 27.58936310585079 28.57867764012069 21.77752805629339 48-49 22.559977451202997 27.631327168951557 28.106918879136884 21.701776500708565 50-51 22.797242271341137 27.810399456952624 27.929307891850335 21.463050379855908 52-53 21.861408597558608 27.894535431236722 28.418613808670766 21.8254421625339 54-55 21.638238127863353 27.86835219488758 28.59475422108965 21.898655456159414 56-57 21.747766030865918 28.029861876607075 28.373443605931943 21.848928486595064 58-59 21.811713804892577 27.868505687337148 28.528084039879076 21.791696467891196 60-61 21.674560150661215 27.952722031917904 28.566391163435316 21.806326653985565 62-63 21.770765016999313 27.876870727967578 28.472565664038996 21.879798590994113 64-65 21.518480115691766 27.827100516497406 28.479907346124616 22.174512021686212 66-67 21.622486456463175 27.90966626810366 28.35846258635571 22.10938468907746 68-69 21.68356826766426 27.831391093719198 28.400184442898606 22.084856195717933 70-71 21.58637039732082 27.737459058542484 28.22232533249109 22.453845211645607 72-73 21.69813228608305 27.89705646469367 28.214367537309816 22.19044371191346 74-75 21.693156319082643 28.043546259928764 28.057910458356584 22.20538696263201 76-77 21.796557250796162 27.806340153612393 28.024922118380065 22.372180477211387 78-79 21.64207291628629 27.933570253237068 28.03447817364469 22.389878656831954 80-81 21.789241183789617 27.996040513139693 27.92037230097672 22.29434600209397 82-83 21.86806368053672 27.880868117697283 28.039490904484403 22.211577297281586 84-85 21.911871681890986 27.9118837328028 27.820680241105876 22.355564344200335 86-87 21.69112069734108 28.078375431208897 27.787862369780058 22.442641501669968 88-89 21.76359556369214 27.863658110442074 27.71584059100274 22.656905734863045 90-91 21.806192001492047 27.839825985500237 27.768774763560184 22.58520724944753 92-93 21.882318918782666 27.73263410579104 27.77628966014317 22.60875731528313 94-95 21.709461076980563 27.638282816206278 27.830879160842496 22.821376945970666 96-97 21.763669783725252 27.51843694560463 27.709396693619464 23.008496577050657 98-99 21.615474070704714 27.61909589854033 27.5568310962545 23.208598934500458 100-101 21.948127723377283 27.745640182461965 27.4454358989921 22.860796195168646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23135.0 1 24899.5 2 18813.0 3 11587.5 4 14758.0 5 18593.5 6 19703.5 7 18835.5 8 17544.0 9 16070.0 10 14319.5 11 12407.0 12 10504.5 13 9008.0 14 7828.0 15 6841.0 16 6183.5 17 5902.0 18 5869.5 19 6064.5 20 6598.0 21 7442.5 22 8512.5 23 9994.5 24 11928.5 25 14238.5 26 17077.0 27 20140.0 28 23533.5 29 27700.0 30 32004.5 31 36409.0 32 40967.5 33 44344.5 34 47242.5 35 50466.0 36 53510.5 37 56274.0 38 58341.5 39 59583.0 40 60865.0 41 61945.0 42 62742.5 43 63439.5 44 63939.5 45 64505.5 46 64395.5 47 64026.0 48 62990.5 49 60804.5 50 58273.5 51 55414.5 52 52642.0 53 49779.0 54 46043.5 55 41878.5 56 37081.0 57 32062.5 58 27504.5 59 23122.5 60 19015.0 61 15303.5 62 12209.0 63 9441.0 64 7035.0 65 5273.0 66 3859.0 67 2804.5 68 2093.0 69 1594.0 70 1174.5 71 823.0 72 614.0 73 468.5 74 348.5 75 251.5 76 167.5 77 106.0 78 76.0 79 51.5 80 26.0 81 14.0 82 10.0 83 4.5 84 4.0 85 4.5 86 2.5 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.972690906210392 2 20.747682984790874 3 16.542894486692013 4 21.70048122623574 5 21.532794676806084 6 15.55776695183777 7 14.390991365652726 8 11.57002534854246 9 2.9002297211660326 10-11 6.227919043092522 12-13 3.4036606859949305 14-15 7.097220571926489 16-17 11.054935044359949 18-19 14.035468155893534 20-21 14.427875475285171 22-23 12.397640407160964 24-25 14.862984196768062 26-27 12.49086561311787 28-29 12.47499801964512 30-31 13.28285903833967 32-33 11.802766555766793 34-35 18.651007010456276 36-37 23.307687737642585 38-39 22.451778358681874 40-41 17.534284893852977 42-43 17.443040042775664 44-45 22.723309767110266 46-47 12.238518892585551 48-49 20.957229285487962 50-51 20.537443559885933 52-53 10.732038181242078 54-55 12.31010872148289 56-57 11.834650269328263 58-59 12.816188410963244 60-61 12.247826560519645 62-63 12.205570738276299 64-65 13.26558044201521 66-67 11.577179380544994 68-69 12.601196134347276 70-71 11.278096284854247 72-73 10.643739108048162 74-75 10.730849968314322 76-77 10.8041973621673 78-79 11.19521843314322 80-81 11.147566143852979 82-83 9.720151101077313 84-85 9.617197401774398 86-87 9.763273328580482 88-89 11.108949223700886 90-91 8.683658111533585 92-93 10.634530457858048 94-95 10.761520714512042 96-97 10.320792736058301 98-99 10.149838601077313 100-101 10.268511367237009 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2019840.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.28941780618773 #Duplication Level Percentage of deduplicated Percentage of total 1 98.88511684188187 96.20475447243535 2 0.8109005841102426 1.5778409145356616 3 0.14921775942514726 0.4355192682244907 4 0.05654787009610984 0.22006037439321838 5 0.02948887050824985 0.14344775217548436 6 0.016917667941609073 0.09875460388065317 7 0.011312083619889314 0.07703822206877609 8 0.008020717158539968 0.06242647221939631 9 0.006061831426456263 0.05307768452872641 >10 0.022973409161615315 0.37721172547220894 >50 0.0019594715952375376 0.13231122714418816 >100 0.0012785429666798188 0.21884176096387 >500 0.0 0.0 >1k 2.043501082931191E-4 0.39871552195792526 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3582 0.1773407794676806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.901774397972116E-5 0.0 5 0.0 0.0 0.0 9.901774397972116E-5 0.0 6 0.0 0.0 0.0 9.901774397972116E-5 0.0 7 0.0 0.0 0.0 9.901774397972116E-5 0.0 8 0.0 0.0 0.0 9.901774397972116E-5 0.0 9 0.0 0.0 0.0 9.901774397972116E-5 4.950887198986058E-5 10-11 0.0 0.0 0.0 9.901774397972116E-5 9.901774397972116E-5 12-13 0.0 0.0 0.0 9.901774397972116E-5 1.7328105196451202E-4 14-15 0.0 0.0 0.0 9.901774397972116E-5 1.9803548795944233E-4 16-17 0.0 0.0 0.0 9.901774397972116E-5 1.9803548795944233E-4 18-19 0.0 0.0 0.0 9.901774397972116E-5 1.9803548795944233E-4 20-21 0.0 0.0 0.0 1.2377217997465147E-4 2.4754435994930294E-4 22-23 0.0 0.0 0.0 3.218076679340938E-4 2.970532319391635E-4 24-25 0.0 0.0 0.0 3.465621039290241E-4 2.970532319391635E-4 26-27 4.950887198986058E-5 4.950887198986058E-5 0.0 5.94106463878327E-4 3.713165399239544E-4 28-29 4.950887198986058E-5 4.950887198986058E-5 0.0 0.0014605117237008873 3.9607097591888465E-4 30-31 4.950887198986058E-5 4.950887198986058E-5 0.0 0.0046043250950570346 3.9607097591888465E-4 32-33 4.950887198986058E-5 4.950887198986058E-5 0.0 0.012104919201520913 3.9607097591888465E-4 34-35 4.950887198986058E-5 4.950887198986058E-5 0.0 0.021585868187579214 3.9607097591888465E-4 36-37 4.950887198986058E-5 7.426330798479087E-5 0.0 0.03668607414448669 3.9607097591888465E-4 38-39 4.950887198986058E-5 9.901774397972116E-5 0.0 0.058791785487959436 3.9607097591888465E-4 40-41 4.950887198986058E-5 9.901774397972116E-5 0.0 0.08248178073510773 3.9607097591888465E-4 42-43 4.950887198986058E-5 9.901774397972116E-5 0.0 0.10924132604562738 3.9607097591888465E-4 44-45 4.950887198986058E-5 9.901774397972116E-5 0.0 0.14070421419518375 3.9607097591888465E-4 46-47 4.950887198986058E-5 9.901774397972116E-5 0.0 0.17956867870722432 3.9607097591888465E-4 48-49 4.950887198986058E-5 9.901774397972116E-5 0.0 0.22033923479087453 3.9607097591888465E-4 50-51 4.950887198986058E-5 9.901774397972116E-5 0.0 0.269179737008872 3.9607097591888465E-4 52-53 4.950887198986058E-5 9.901774397972116E-5 0.0 0.32970433301647656 4.2082541191381496E-4 54-55 4.950887198986058E-5 9.901774397972116E-5 0.0 0.398793963878327 4.950887198986059E-4 56-57 4.950887198986058E-5 9.901774397972116E-5 0.0 0.4795924429657794 4.950887198986059E-4 58-59 4.950887198986058E-5 9.901774397972116E-5 0.0 0.5695748178073511 4.950887198986059E-4 60-61 4.950887198986058E-5 9.901774397972116E-5 0.0 0.660473106780735 5.445975918884664E-4 62-63 4.950887198986058E-5 9.901774397972116E-5 0.0 0.753921102661597 5.445975918884664E-4 64-65 9.901774397972116E-5 9.901774397972116E-5 0.0 0.8532358998732572 5.445975918884664E-4 66-67 9.901774397972116E-5 9.901774397972116E-5 0.0 0.9696065034854247 5.693520278833967E-4 68-69 9.901774397972116E-5 9.901774397972116E-5 0.0 1.0949877217997466 5.94106463878327E-4 70-71 9.901774397972116E-5 9.901774397972116E-5 0.0 1.229379554816223 6.188608998732572E-4 72-73 9.901774397972116E-5 9.901774397972116E-5 0.0 1.370405576679341 6.931242078580482E-4 74-75 9.901774397972116E-5 9.901774397972116E-5 0.0 1.511753406210393 6.931242078580482E-4 76-77 9.901774397972116E-5 9.901774397972116E-5 0.0 1.6727315034854247 6.931242078580482E-4 78-79 9.901774397972116E-5 9.901774397972116E-5 0.0 1.8560876108998734 6.931242078580482E-4 80-81 9.901774397972116E-5 9.901774397972116E-5 0.0 2.0509297766159698 7.178786438529784E-4 82-83 9.901774397972116E-5 9.901774397972116E-5 0.0 2.256663894169835 7.921419518377693E-4 84-85 9.901774397972116E-5 9.901774397972116E-5 0.0 2.4629673637515843 7.921419518377693E-4 86-87 9.901774397972116E-5 9.901774397972116E-5 0.0 2.6844205481622305 7.921419518377693E-4 88-89 9.901774397972116E-5 9.901774397972116E-5 0.0 2.9235731543092522 7.921419518377693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1790 0.0 53.05616 1 GTATCAA 3425 0.0 46.36108 1 ATCAACG 4305 0.0 36.990036 3 TCAACGC 4360 0.0 36.855747 4 TATCAAC 4410 0.0 35.318916 2 CAACGCA 4440 0.0 35.124035 5 AACGCAG 4795 0.0 32.593517 6 ACGCAGA 5435 0.0 28.108644 7 GTACATG 3730 0.0 28.020864 1 CGCAGAG 5635 0.0 26.799095 8 TACATGG 4055 0.0 25.400791 2 ACATGGG 4235 0.0 23.60469 3 GCAGAGT 6390 0.0 22.555626 9 CATGGGG 3005 0.0 22.392475 4 AGAGTAC 5665 0.0 20.38178 10-11 GTGGTAT 850 0.0 20.099583 1 GAGTACT 4225 0.0 19.716131 12-13 AGTACTT 4625 0.0 18.904057 12-13 CAGAGTA 6020 0.0 18.738102 10-11 GAGTACA 3000 0.0 17.754631 1 >>END_MODULE