FastQCFastQC Report
Sat 18 Jun 2016
SRR1930942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019840
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54310.26888268377693286No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42890.21234355196451202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34010.16837967363751583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24500.058.725221
GTATCAA47650.040.1596761
ATCAACG59150.031.7040773
TCAACGC59600.031.6232364
CAACGCA59400.031.5604845
TATCAAC60000.031.3348392
AACGCAG63700.029.5790426
ACGCAGA71550.026.0021617
CGCAGAG73750.025.1621518
TATTACG1252.2641268E-422.7889752
CCGATCG2154.4648914E-822.0746889
GTGGTAT11250.021.94821
GCAGAGT84300.021.7879529
GTACATG50100.021.5147481
CCGTACG1553.9229086E-521.441242
TGTACGC1353.809739E-421.1009042
TACATGG51050.020.367232
ACATGGG49000.020.3467963
GAGTACT58900.019.86253512-13
AGAGTAC78650.019.64198110-11
Produced by FastQC (version 0.11.5)