##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930942_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019840 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.259072005703423 31.0 31.0 34.0 30.0 34.0 2 31.383237286121673 31.0 31.0 34.0 30.0 34.0 3 31.45928093314322 31.0 31.0 34.0 30.0 34.0 4 35.32397318599493 37.0 35.0 37.0 33.0 37.0 5 35.112755465779465 37.0 35.0 37.0 32.0 37.0 6 35.14834442332066 37.0 35.0 37.0 32.0 37.0 7 35.02941668647021 37.0 35.0 37.0 32.0 37.0 8 35.01091720136248 37.0 35.0 37.0 32.0 37.0 9 36.521026417934095 38.0 35.0 39.0 32.0 39.0 10-11 36.449328164607095 38.0 35.0 39.0 32.0 39.0 12-13 36.342380832145125 38.0 35.0 39.0 32.0 39.0 14-15 37.355271952233835 39.0 36.0 41.0 32.0 41.0 16-17 37.33163790201204 39.0 36.0 41.0 32.0 41.0 18-19 37.33684598780101 39.0 36.0 41.0 32.0 41.0 20-21 37.30481102463561 39.0 36.0 40.5 31.5 41.0 22-23 37.18530081590622 39.0 36.0 40.0 31.0 41.0 24-25 37.063283230354884 39.0 36.0 40.0 31.0 41.0 26-27 36.7879802360583 39.0 35.5 40.0 30.5 41.0 28-29 36.60351092165716 38.5 35.0 40.0 30.0 41.0 30-31 36.35603760693916 38.0 35.0 40.0 30.0 41.0 32-33 36.11005228136882 38.0 35.0 40.0 30.0 41.0 34-35 36.26930573708809 38.0 35.0 40.0 30.0 41.0 36-37 36.30834001703106 38.0 35.0 40.0 30.0 41.0 38-39 36.27085536478137 38.0 35.0 40.0 30.0 41.0 40-41 36.17504752851711 38.0 35.0 40.0 30.0 41.0 42-43 36.031153952788344 38.0 35.0 40.0 29.5 41.0 44-45 35.84029378564639 38.0 35.0 40.0 28.5 41.0 46-47 35.601165438846635 38.0 34.0 40.0 28.0 41.0 48-49 35.39397724572243 38.0 34.0 40.0 27.0 41.0 50-51 35.128349522734474 38.0 34.0 40.0 26.5 41.0 52-53 34.924591304261725 38.0 33.5 40.0 26.0 41.0 54-55 34.7587977265526 38.0 33.0 40.0 26.0 41.0 56-57 34.50777314044677 37.5 33.0 40.0 26.0 41.0 58-59 34.27008426410012 37.0 33.0 40.0 25.0 41.0 60-61 33.9624499960393 37.0 32.5 40.0 24.5 41.0 62-63 33.66616192371673 36.5 32.0 39.5 23.5 41.0 64-65 33.357806311391 36.0 32.0 39.0 23.0 41.0 66-67 33.01634560163181 36.0 31.0 39.0 22.5 40.0 68-69 32.649582640209125 35.0 31.0 39.0 22.0 40.0 70-71 32.25126693203422 35.0 31.0 38.0 21.5 40.0 72-73 31.828187133634348 35.0 30.5 37.5 20.5 40.0 74-75 31.30706937183143 34.0 30.0 37.0 19.0 39.0 76-77 29.93456511406844 33.5 28.5 35.5 18.0 38.0 78-79 30.6925682232256 34.0 30.0 36.0 18.5 39.0 80-81 30.68091036913815 34.0 30.0 36.0 18.0 37.5 82-83 30.397883495722432 34.0 30.0 35.0 18.0 37.0 84-85 30.079229295389734 34.0 30.0 35.0 16.5 37.0 86-87 29.73881792617237 34.0 29.0 35.0 12.0 36.0 88-89 29.39022496831432 34.0 29.0 35.0 7.5 36.0 90-91 29.049841571609633 34.0 29.0 35.0 4.5 35.5 92-93 28.693082620405576 34.0 29.0 35.0 2.0 35.0 94-95 28.275688915953737 33.0 29.0 35.0 2.0 35.0 96-97 27.82061202867554 33.0 27.0 35.0 2.0 35.0 98-99 27.345085996910647 33.0 27.0 35.0 2.0 35.0 100-101 26.151822421577947 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4549234931679216 1101 2 0.5419847162316032 1101 3 0.5606508111025832 1101 4 0.3434846368150204 1101 5 0.48779816089950145 1101 6 0.37631235975919 1101 7 0.4653985749646381 1101 8 0.45239881623155753 1101 9 0.655277257377314 1101 10-11 0.5246101233597216 1101 12-13 0.5431200466592827 1101 14-15 0.7347916374243013 1101 16-17 0.6499088431275268 1101 18-19 0.661378898138345 1101 20-21 0.7345898131780828 1101 22-23 0.7697063656233141 1101 24-25 0.8775328035839252 1101 26-27 0.7504678383683441 1101 28-29 0.7612782426065792 1101 30-31 0.7635825026448586 1101 32-33 0.6942548161541922 1101 34-35 0.6415828357458082 1101 36-37 0.7833530226163887 1101 38-39 0.8982837734941214 1101 40-41 0.7711522007553597 1101 42-43 0.7842945056130901 1101 44-45 0.8559044227769022 1101 46-47 0.7565820432898249 1101 48-49 0.7810663817311436 1101 50-51 0.7822955499961495 1101 52-53 0.8814184027369834 1101 54-55 0.6922788127080679 1101 56-57 0.8058122054473458 1101 58-59 0.6998039877450779 1101 60-61 0.6550895272456358 1101 62-63 0.61633614746912 1101 64-65 0.5272779707443291 1101 66-67 0.7662683186984722 1101 68-69 0.6174552883250968 1101 70-71 0.5462255067119983 1101 72-73 0.5223654246664609 1101 74-75 0.779279847248219 1101 76-77 0.755992045827 1101 78-79 0.5759067102991686 1101 80-81 0.550539404140391 1101 82-83 0.8248857599147179 1101 84-85 0.9578463676396076 1101 86-87 0.7179419173347164 1101 88-89 0.5261570072136088 1101 90-91 0.6548090694191266 1101 92-93 0.6892307203191379 1101 94-95 0.7606698424695146 1101 96-97 0.7676930956077435 1101 98-99 0.9419172532792324 1101 100-101 1.3328405246800266 1102 1 0.5320849896943471 1102 2 0.5599421688050406 1102 3 0.5422659858195864 1102 4 0.340545613854097 1102 5 0.4853312978820341 1102 6 0.5313314489250018 1102 7 0.6583598043719832 1102 8 0.632130208695628 1102 9 0.6699578887480726 1102 10-11 0.6444888368564605 1102 12-13 0.7448456475323937 1102 14-15 0.8636878482460588 1102 16-17 0.8135571621801958 1102 18-19 0.8441701945304558 1102 20-21 0.9004130983506613 1102 22-23 0.9566215412761636 1102 24-25 0.9547322912714478 1102 26-27 0.9096072416419574 1102 28-29 1.0232276325866252 1102 30-31 0.958642715849912 1102 32-33 1.0164969586685118 1102 34-35 1.0501755450971615 1102 36-37 1.0074170919800025 1102 38-39 1.1248305262414036 1102 40-41 1.10448241457987 1102 42-43 1.0997434720084058 1102 44-45 1.2932046349480615 1102 46-47 1.269022644499394 1102 48-49 1.208147974267142 1102 50-51 1.3288098776773793 1102 52-53 1.1900240580499712 1102 54-55 1.1823241258020332 1102 56-57 1.0551061937527564 1102 58-59 1.164098312511797 1102 60-61 1.1132804215776275 1102 62-63 0.9185364484571608 1102 64-65 0.9498977743667893 1102 66-67 0.9542079187685246 1102 68-69 1.0108848451364807 1102 70-71 1.0680834594318256 1102 72-73 1.1259496066176098 1102 74-75 1.2632015645110926 1102 76-77 1.2742903047399956 1102 78-79 1.313931472960249 1102 80-81 1.2262378287438658 1102 82-83 1.3167861780625962 1102 84-85 1.4071185862298314 1102 86-87 1.2300732415261066 1102 88-89 1.4505849004354943 1102 90-91 1.3443159998536345 1102 92-93 1.4099162502100455 1102 94-95 1.4712179713658422 1102 96-97 1.5388248712366241 1102 98-99 1.466452301629392 1102 100-101 1.6285885672996478 1103 1 0.5071951700305277 1103 2 0.6237953181450564 1103 3 0.5745661721427666 1103 4 0.4545331471155123 1103 5 0.4991029349438705 1103 6 0.5929071686868639 1103 7 0.6100570609040972 1103 8 0.6171907884101557 1103 9 0.77640649608486 1103 10-11 0.6759748876277101 1103 12-13 0.6779765109921385 1103 14-15 0.8199224131815299 1103 16-17 0.772456589564932 1103 18-19 0.797749769315196 1103 20-21 0.7934152621391135 1103 22-23 0.8570824459867694 1103 24-25 0.9271615940963045 1103 26-27 0.937707861863629 1103 28-29 1.0301247494147532 1103 30-31 1.0821560299607782 1103 32-33 1.08556915347134 1103 34-35 0.9607687188634344 1103 36-37 1.0002741549185359 1103 38-39 1.0332711001598014 1103 40-41 0.9745515579649151 1103 42-43 1.0372410724913976 1103 44-45 0.9730481875363779 1103 46-47 0.7542231914686113 1103 48-49 0.7750010957453455 1103 50-51 0.7795471080237704 1103 52-53 0.6866746065439031 1103 54-55 0.7625725303837427 1103 56-57 0.8137991365291839 1103 58-59 0.893596784389679 1103 60-61 0.8784342352794496 1103 62-63 0.9192371622682174 1103 64-65 0.8443680586385298 1103 66-67 0.8677545551776902 1103 68-69 0.9940078217266262 1103 70-71 0.917597490052195 1103 72-73 0.8363411222326249 1103 74-75 0.784035843939705 1103 76-77 0.9620282956921429 1103 78-79 1.0036227530222206 1103 80-81 0.8847075259429218 1103 82-83 1.0402745042889698 1103 84-85 1.042403080644192 1103 86-87 1.070417113428423 1103 88-89 1.0595378905243642 1103 90-91 1.1410212408814147 1103 92-93 1.2149393415163061 1103 94-95 1.2079513023736972 1103 96-97 1.1534116530186829 1103 98-99 1.2212324137304371 1103 100-101 1.5026841824853499 1104 1 0.4848564376899702 1104 2 0.56988942326835 1104 3 0.4528819512561597 1104 4 0.25621255046629443 1104 5 0.5343279281125533 1104 6 0.45647377694738367 1104 7 0.48972032550984324 1104 8 0.41944324541818645 1104 9 0.6954816069076131 1104 10-11 0.6967281715165541 1104 12-13 0.6442767470772992 1104 14-15 0.664443890714594 1104 16-17 0.6845177826302731 1104 18-19 0.7145957985389941 1104 20-21 0.6875845544923109 1104 22-23 0.6562986898887644 1104 24-25 0.764342157152285 1104 26-27 0.7969140821830152 1104 28-29 0.6943871807940099 1104 30-31 0.7344578278599769 1104 32-33 0.9840402333634302 1104 34-35 0.8898314750378375 1104 36-37 0.8190655232411714 1104 38-39 0.8912434744408912 1104 40-41 0.9150044840351654 1104 42-43 0.8887415017960052 1104 44-45 0.8968223383485565 1104 46-47 0.8100567481957412 1104 48-49 0.7440701198964774 1104 50-51 0.7732894915902762 1104 52-53 0.7216920701112883 1104 54-55 0.7725132306617866 1104 56-57 0.8851239200314538 1104 58-59 0.6409798393549124 1104 60-61 0.5819000119970283 1104 62-63 0.6395924814364449 1104 64-65 0.6383798745516813 1104 66-67 0.5989545101104241 1104 68-69 0.6469364172983845 1104 70-71 0.6932201112319873 1104 72-73 0.6369411126493532 1104 74-75 0.6249720128849887 1104 76-77 0.8443505940193461 1104 78-79 0.8746850326231943 1104 80-81 0.7175717322992199 1104 82-83 0.9317996558406172 1104 84-85 1.061612681008878 1104 86-87 1.0258571733109108 1104 88-89 1.0370499052256186 1104 90-91 1.1125718787516483 1104 92-93 0.9999759068722653 1104 94-95 0.8929077697212939 1104 96-97 0.846075530176698 1104 98-99 1.1855839436218254 1104 100-101 1.7562817939199995 1105 1 0.25659836150945736 1105 2 0.35808589899945176 1105 3 0.3381436885954585 1105 4 0.10834693501065118 1105 5 0.1986416384096401 1105 6 0.32561737573087157 1105 7 0.3025046787374137 1105 8 0.36328546045153587 1105 9 0.4908034595701807 1105 10-11 0.37146327021286396 1105 12-13 0.3947603816329561 1105 14-15 0.4924407784067739 1105 16-17 0.37295123283657716 1105 18-19 0.49416401425678913 1105 20-21 0.4832127951343992 1105 22-23 0.5374608333777715 1105 24-25 0.5743341503852761 1105 26-27 0.4320140699145796 1105 28-29 0.65548527014694 1105 30-31 0.5817038874329015 1105 32-33 0.6580724799718567 1105 34-35 0.5986967095263509 1105 36-37 0.7071896797863744 1105 38-39 0.7390590476027299 1105 40-41 0.670746433682929 1105 42-43 0.5872902752755209 1105 44-45 0.6123535292260058 1105 46-47 0.6339885873630422 1105 48-49 0.745501329499433 1105 50-51 0.6493679837352531 1105 52-53 0.6006707553226676 1105 54-55 0.71991845701438 1105 56-57 0.8514825844510625 1105 58-59 0.7333617879695495 1105 60-61 0.6547312435540249 1105 62-63 0.5682441160437577 1105 64-65 0.6028585629915852 1105 66-67 0.6870789895091391 1105 68-69 0.6435538810903623 1105 70-71 0.467765694918846 1105 72-73 0.5019044042054475 1105 74-75 0.6989768169451906 1105 76-77 0.7885095283444841 1105 78-79 0.8591234935159484 1105 80-81 0.8372829075505592 1105 82-83 1.041004209366502 1105 84-85 0.9284023548622606 1105 86-87 0.9039041032969024 1105 88-89 0.7928754739320745 1105 90-91 0.7566781879549112 1105 92-93 0.812027059782146 1105 94-95 0.8202399020395781 1105 96-97 0.6897815010294828 1105 98-99 0.9293686907306729 1105 100-101 1.2455272207000547 1106 1 0.12353204368563198 1106 2 0.15692739622432228 1106 3 0.145214216220257 1106 4 -0.028515740420154145 1106 5 0.2080539351198638 1106 6 0.14265684126918643 1106 7 0.12320591745250908 1106 8 0.05843019254776749 1106 9 0.18332864031707174 1106 10-11 0.15683609600140613 1106 12-13 0.09414559995581584 1106 14-15 0.14689310052653326 1106 16-17 0.10714375317228786 1106 18-19 0.19613486865259233 1106 20-21 0.12313107546838609 1106 22-23 0.2446654070950487 1106 24-25 0.2303035082022049 1106 26-27 0.331122582648959 1106 28-29 0.19635694658443725 1106 30-31 0.251577431902291 1106 32-33 0.3849925960374705 1106 34-35 0.36078750556633565 1106 36-37 0.3579091681502078 1106 38-39 0.22132297305372362 1106 40-41 0.19331893431547797 1106 42-43 0.43441151431362357 1106 44-45 0.30707889623774776 1106 46-47 0.1420997218021185 1106 48-49 0.24088239185508797 1106 50-51 0.2766918530914566 1106 52-53 0.11712612443392345 1106 54-55 0.24133945326217088 1106 56-57 0.16380728823073554 1106 58-59 0.3522337551094168 1106 60-61 0.20523055179005212 1106 62-63 0.19099372978643459 1106 64-65 0.24436924630365553 1106 66-67 0.25418440141825016 1106 68-69 0.23759394418353708 1106 70-71 0.08256136883692733 1106 72-73 0.08379795330453987 1106 74-75 0.2803605602226966 1106 76-77 0.3767979912287913 1106 78-79 0.32435226013039653 1106 80-81 0.26670360232922263 1106 82-83 0.316694492908983 1106 84-85 0.26628141190990817 1106 86-87 0.33173885515012813 1106 88-89 0.3926585238305975 1106 90-91 0.4260241614579279 1106 92-93 0.6875784521926604 1106 94-95 0.610979416566618 1106 96-97 0.6902358580658294 1106 98-99 0.6959637584804739 1106 100-101 0.8425884350822876 1107 1 0.07688077347777877 1107 2 0.16062254633979478 1107 3 0.20618419312556924 1107 4 0.06478679999555226 1107 5 0.08288072495820131 1107 6 0.07845360801283618 1107 7 0.09733986664419092 1107 8 -0.05750514232521198 1107 9 -0.04299930425567311 1107 10-11 0.10718251632472686 1107 12-13 0.06689386785420481 1107 14-15 0.13049587188911715 1107 16-17 0.01753636287205751 1107 18-19 0.07188544601979885 1107 20-21 0.23005948193489445 1107 22-23 0.19177857106733853 1107 24-25 0.09658526339850937 1107 26-27 0.11818955724479707 1107 28-29 0.2515532514343164 1107 30-31 0.2679746605397071 1107 32-33 0.24296026347396094 1107 34-35 0.2804179905547812 1107 36-37 0.3267313390509017 1107 38-39 0.3732860215525662 1107 40-41 0.21040900359954406 1107 42-43 0.274134378054967 1107 44-45 0.3271712749906399 1107 46-47 0.2492590751508459 1107 48-49 0.22771841956870986 1107 50-51 0.2596017838073834 1107 52-53 0.2711676948727231 1107 54-55 0.46050804448618976 1107 56-57 0.37766410116375226 1107 58-59 0.40673721931264595 1107 60-61 0.3063852862011345 1107 62-63 0.1124717898326324 1107 64-65 0.06677109387870672 1107 66-67 0.1170019533812976 1107 68-69 0.3793953298648347 1107 70-71 0.32898169216256434 1107 72-73 0.36347462766943295 1107 74-75 0.44664231541900534 1107 76-77 0.4102852891502664 1107 78-79 0.4585323986985266 1107 80-81 0.46970591179804444 1107 82-83 0.5721217446410805 1107 84-85 0.57598118096303 1107 86-87 0.40379428240186144 1107 88-89 0.5289171843386811 1107 90-91 0.6334144616888757 1107 92-93 0.6076708309455441 1107 94-95 0.5142126729176617 1107 96-97 0.5424298534468868 1107 98-99 0.611437199589016 1107 100-101 0.8569071417797467 1108 1 0.2524078929413065 1108 2 0.2878997189420289 1108 3 0.2426079415575657 1108 4 0.19177014032226936 1108 5 0.19506209920778872 1108 6 0.20394972530785793 1108 7 0.20037609678050217 1108 8 0.320284970240742 1108 9 0.3478559921591966 1108 10-11 0.21733966372874391 1108 12-13 0.24187263990401675 1108 14-15 0.34597713237231176 1108 16-17 0.5290483545989346 1108 18-19 0.4199911199122255 1108 20-21 0.3714744142973032 1108 22-23 0.48565589058571135 1108 24-25 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-0.6855120984788527 2316 88-89 -0.9531847737248604 2316 90-91 -0.5994380395983328 2316 92-93 -0.7922963912592245 2316 94-95 -0.5187245043405575 2316 96-97 -0.44761034032451263 2316 98-99 -0.39286543233136584 2316 100-101 -0.558566162754655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 11.0 4 33.0 5 57.0 6 166.0 7 379.0 8 866.0 9 1567.0 10 2607.0 11 4095.0 12 5685.0 13 7245.0 14 8549.0 15 10733.0 16 12698.0 17 14196.0 18 12942.0 19 11569.0 20 11542.0 21 12261.0 22 13659.0 23 15807.0 24 19169.0 25 24150.0 26 30536.0 27 38942.0 28 49315.0 29 60618.0 30 73661.0 31 88420.0 32 106488.0 33 127674.0 34 156525.0 35 195461.0 36 250722.0 37 323762.0 38 278557.0 39 49148.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.67485979188888 22.104899336369733 14.966145051889132 23.25409581985226 2 15.37855986786634 22.347659860786337 39.59152835491909 22.682251916428232 3 18.042359530201143 26.643142396267276 31.39494929590575 23.919548777625835 4 12.723752771604898 18.277957364953444 39.05846903900033 29.939820824441334 5 12.595993367663446 38.549738315804376 35.993496389000704 12.860771927531475 6 30.0162686314447 39.18126107143246 17.735184702931623 13.067285594191217 7 25.68540082382763 33.43111335551331 23.491464670468947 17.392021150190114 8 24.63333729404309 36.757663973384034 21.088056479721164 17.52094225285171 9 25.252210696001022 16.07345932027256 21.260456897802253 37.41387308592417 10-11 23.806044043092523 27.166334957224336 29.475453501267427 19.552167498415717 12-13 24.493202431875794 24.615043765842838 29.600958491761727 21.290795310519645 14-15 21.636446856297244 26.398359869682714 27.014720724097135 24.950472549922903 16-17 21.25933242237009 29.318139060519645 27.862924786121674 21.559603730988595 18-19 21.182197599809886 28.280334085868187 29.891996395754116 20.645471918567807 20-21 21.72612682192649 27.425860464195186 29.900784220532316 20.947228493346007 22-23 21.50521823510773 27.29956333174905 29.79812757446134 21.397090858681874 24-25 21.467024222661042 27.51750338581564 29.796093569561894 21.219378821961424 26-27 21.4601853706187 27.672212701162476 29.669894145014474 21.197707783204358 28-29 21.444739627633503 27.770816273384302 29.619776437516276 21.16466766146592 30-31 21.74593841869101 27.533318892155346 29.51553148692558 21.205211202228067 32-33 21.656761600465895 27.63364906402448 29.29416932389195 21.415420011617677 34-35 21.728276650233816 27.680274439820714 29.483811100789687 21.107637809155786 36-37 21.761803480296344 27.557420730874117 29.456864643920177 21.22391114490937 38-39 21.61984865516781 27.613364428444154 29.458747529797762 21.308039386590274 40-41 21.712741716083627 27.63569705453875 29.41401448904039 21.237546740337233 42-43 21.683103875883784 27.653676579990993 29.149946612182127 21.513272931943096 44-45 21.58501533670188 27.814290983900797 29.19390096872501 21.406792710672313 46-47 21.828595336355285 27.77586877031587 28.895806977889897 21.499728915438947 48-49 21.73929727488225 27.88614293406614 28.726689814385008 21.647869976666605 50-51 21.76831875491693 27.901819152239543 28.742223439157062 21.58763865368647 52-53 21.855548719899584 27.915630173179856 28.66477844053921 21.564042666381354 54-55 21.61556899541187 27.93583102721806 28.766181080537045 21.68241889683302 56-57 21.737134787704377 27.979741663378082 28.585971668829625 21.697151880087915 58-59 21.680394750818373 28.03535009523866 28.70042371485993 21.583831439083035 60-61 21.61395647542427 28.176375904285262 28.680811604770284 21.528856015520184 62-63 21.657076792173356 28.04365569220294 28.613200576631364 21.686066938992344 64-65 21.431799788981337 27.984454595057763 28.624820413602524 21.958925202358373 66-67 21.480576178861366 28.079216292077056 28.520675220289615 21.91953230877196 68-69 21.54658127907435 28.01708511757603 28.60604937259002 21.830284230759606 70-71 21.54595833182606 27.855872498935824 28.539473169107293 22.058696000130823 72-73 21.55674174894273 28.025428951926468 28.47853357695768 21.939295722173117 74-75 21.490094500104348 28.198309018641694 28.292831456988072 22.01876502426589 76-77 21.530647282463374 28.080388366002136 28.298599688610032 22.090364662924458 78-79 21.447028935528447 28.12954121336022 28.33702283065232 22.08640702045901 80-81 21.591006140391052 28.208642096025766 28.184134992059946 22.01621677152324 82-83 21.594061905893536 28.03803766634981 28.377643773764262 21.990256653992397 84-85 21.617256812420784 28.122722591888465 28.071060083967048 22.188960511723703 86-87 21.448976156527248 28.234587888149555 28.14438272338403 22.172053231939163 88-89 21.48801574384078 28.046365116005617 28.12968865069591 22.33593048945769 90-91 21.424186055262638 27.967704130462877 28.256608280136515 22.351501534137974 92-93 21.539157084403968 27.927948449151575 28.221123349359207 22.311771117085254 94-95 21.29324158499812 27.874634153224537 28.25838492464974 22.573739337127602 96-97 21.27666052756654 27.71098205798479 28.316228018060833 22.696129396387832 98-99 21.141823114702156 27.817921221482887 28.186688054499363 22.85356760931559 100-101 21.189029824144487 27.73365712135615 28.175177241761723 22.902135812737644 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 744.0 1 541.5 2 422.0 3 529.0 4 455.5 5 424.5 6 716.5 7 1472.5 8 2283.0 9 2489.0 10 1962.5 11 1429.0 12 1370.5 13 1458.5 14 1599.0 15 1807.0 16 1989.5 17 2178.5 18 2477.0 19 2819.0 20 3292.5 21 3946.5 22 4813.0 23 6117.0 24 7834.0 25 10071.5 26 13032.5 27 16502.5 28 20491.0 29 25630.0 30 31139.5 31 36867.0 32 43294.0 33 47584.0 34 50237.5 35 53382.0 36 57518.0 37 62382.5 38 65191.5 39 66206.5 40 67646.5 41 69253.0 42 70624.0 43 72486.5 44 73975.5 45 75430.5 46 76502.5 47 76602.5 48 76514.0 49 74622.5 50 71172.0 51 68232.0 52 65609.5 53 62881.0 54 59519.5 55 53798.5 56 47212.0 57 40995.5 58 34978.0 59 29461.0 60 23880.0 61 18992.0 62 15311.0 63 11406.0 64 7907.0 65 5724.5 66 4114.5 67 2990.0 68 2140.5 69 1522.0 70 1148.5 71 853.0 72 583.5 73 432.0 74 326.0 75 245.5 76 170.5 77 96.0 78 59.0 79 38.0 80 24.5 81 13.5 82 6.5 83 4.0 84 3.5 85 2.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01693203422053232 2 0.0033170944233206594 3 0.0040597275031685675 4 0.03480473700887199 5 0.0019308460076045625 6 9.901774397972116E-5 7 0.0 8 0.0 9 4.950887198986058E-5 10-11 0.0 12-13 0.0 14-15 2.475443599493029E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.168963878326996E-4 26-27 0.0024011802915082383 28-29 9.901774397972116E-5 30-31 0.007550102978453739 32-33 0.024902962610899873 34-35 0.01524873257287706 36-37 5.94106463878327E-4 38-39 7.67387515842839E-4 40-41 0.001856582699619772 42-43 0.009134386882129277 44-45 0.008664052598225603 46-47 0.008664052598225603 48-49 0.010025546577946768 50-51 0.1904111216730038 52-53 0.05020199619771863 54-55 0.019333214512040557 56-57 0.011238513941698352 58-59 0.021759149239543727 60-61 0.051959561153358674 62-63 0.0947104721166033 64-65 0.0994633238276299 66-67 0.10389436787072244 68-69 0.10201303073510773 70-71 0.09154190430925221 72-73 0.07089670468948037 74-75 0.24628188371356147 76-77 0.2953946847275032 78-79 0.0070797686945500635 80-81 8.664052598225602E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.2377217997465144E-4 90-91 0.0068817332065906215 92-93 0.01965502217997465 94-95 0.001955600443599493 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2019840.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.87727782061793 #Duplication Level Percentage of deduplicated Percentage of total 1 81.0215559705217 52.564579961582815 2 11.31075714090431 14.676222667839742 3 3.3439079651079386 6.508309381766547 4 1.449093224277924 3.7605289479781554 5 0.7708018301013897 2.5003762238064304 6 0.4765979122173263 1.8552225095789971 7 0.3293257243950216 1.4956029560536448 8 0.21712292046488493 1.1269075225779417 9 0.18147564893910934 1.0596281484509573 >10 0.8416642694470498 9.435004379985546 >50 0.039446026723306916 1.7404403749712214 >100 0.016419855222750784 1.903626868594455 >500 0.0015262597310920743 0.661698320632718 >1k 2.2893895966380492E-4 0.44296407592224474 >5k 7.631298655460164E-5 0.26888766025860183 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5431 0.26888268377693286 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4289 0.21234355196451202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3401 0.16837967363751583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.901774397972116E-5 2 0.0 0.0 0.0 0.0 9.901774397972116E-5 3 0.0 0.0 0.0 0.0 9.901774397972116E-5 4 0.0 0.0 0.0 0.0 9.901774397972116E-5 5 0.0 0.0 0.0 0.0 9.901774397972116E-5 6 0.0 0.0 0.0 0.0 9.901774397972116E-5 7 0.0 0.0 0.0 0.0 9.901774397972116E-5 8 0.0 0.0 0.0 0.0 1.4852661596958174E-4 9 0.0 0.0 0.0 0.0 1.9803548795944233E-4 10-11 0.0 0.0 0.0 0.0 2.4754435994930294E-4 12-13 0.0 0.0 0.0 0.0 2.970532319391635E-4 14-15 0.0 0.0 0.0 0.0 3.713165399239544E-4 16-17 0.0 0.0 0.0 0.0 4.950887198986059E-4 18-19 0.0 0.0 0.0 0.0 4.950887198986059E-4 20-21 0.0 0.0 0.0 2.475443599493029E-5 6.188608998732572E-4 22-23 0.0 0.0 0.0 2.722987959442332E-4 6.436153358681876E-4 24-25 0.0 0.0 0.0 3.465621039290241E-4 6.436153358681876E-4 26-27 0.0 0.0 0.0 8.168963878326997E-4 7.67387515842839E-4 28-29 0.0 0.0 0.0 0.002970532319391635 7.921419518377693E-4 30-31 0.0 0.0 0.0 0.009109632446134348 7.921419518377693E-4 32-33 0.0 0.0 0.0 0.024383119455006336 9.40668567807351E-4 34-35 0.0 0.0 0.0 0.0466621118504436 9.40668567807351E-4 36-37 0.0 0.0 0.0 0.08196193757921419 9.40668567807351E-4 38-39 0.0 0.0 0.0 0.13060440430925221 9.901774397972117E-4 40-41 0.0 0.0 0.0 0.180756891634981 9.901774397972117E-4 42-43 0.0 0.0 0.0 0.23816242870722434 9.901774397972117E-4 44-45 0.0 0.0 0.0 0.30633614543726234 0.001014931875792142 46-47 0.0 0.0 0.0 0.38740692332065907 0.0010396863117870722 48-49 0.0 0.0 0.0 0.4731315351711027 0.0011387040557667935 50-51 0.0 0.0 0.0 0.5602671498732573 0.0011387040557667935 52-53 0.0 0.0 0.0 0.6464373415716096 0.0012624762357414448 54-55 0.0 0.0 0.0 0.7448857335234473 0.0012872306717363752 56-57 0.0 0.0 0.0 0.8596968076679341 0.0013119851077313055 58-59 0.0 0.0 0.0 0.9874544518377694 0.0013862484157160964 60-61 0.0 0.0 0.0 1.1176380307351077 0.0013862484157160964 62-63 0.0 0.0 0.0 1.2522031448035489 0.001435757287705957 64-65 0.0 0.0 0.0 1.396323471166033 0.001435757287705957 66-67 2.475443599493029E-5 0.0 0.0 1.563935757287706 0.0014605117237008873 68-69 4.950887198986058E-5 0.0 0.0 1.748999920785805 0.0014852661596958174 70-71 4.950887198986058E-5 0.0 0.0 1.9483969027249683 0.0014852661596958174 72-73 4.950887198986058E-5 0.0 0.0 2.157349096958175 0.0015347750316856781 74-75 4.950887198986058E-5 0.0 0.0 2.3722671102661597 0.0015347750316856781 76-77 4.950887198986058E-5 0.0 0.0 2.618425221799747 0.0015595294676806083 78-79 4.950887198986058E-5 0.0 0.0 2.896368029150824 0.001609038339670469 80-81 4.950887198986058E-5 0.0 0.0 3.1963422845373892 0.0016337927756653993 82-83 4.950887198986058E-5 0.0 0.0 3.5156002455640047 0.0016337927756653993 84-85 4.950887198986058E-5 0.0 0.0 3.845972948352345 0.0016833016476552598 86-87 4.950887198986058E-5 0.0 0.0 4.207065906210393 0.0017575649556400507 88-89 4.950887198986058E-5 0.0 0.0 4.60219621356147 0.0018318282636248415 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2450 0.0 58.72522 1 GTATCAA 4765 0.0 40.159676 1 ATCAACG 5915 0.0 31.704077 3 TCAACGC 5960 0.0 31.623236 4 CAACGCA 5940 0.0 31.560484 5 TATCAAC 6000 0.0 31.334839 2 AACGCAG 6370 0.0 29.579042 6 ACGCAGA 7155 0.0 26.002161 7 CGCAGAG 7375 0.0 25.162151 8 TATTACG 125 2.2641268E-4 22.788975 2 CCGATCG 215 4.4648914E-8 22.074688 9 GTGGTAT 1125 0.0 21.9482 1 GCAGAGT 8430 0.0 21.787952 9 GTACATG 5010 0.0 21.514748 1 CCGTACG 155 3.9229086E-5 21.44124 2 TGTACGC 135 3.809739E-4 21.100904 2 TACATGG 5105 0.0 20.36723 2 ACATGGG 4900 0.0 20.346796 3 GAGTACT 5890 0.0 19.862535 12-13 AGAGTAC 7865 0.0 19.641981 10-11 >>END_MODULE