FastQCFastQC Report
Sat 18 Jun 2016
SRR1930941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677836
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48880.1825354502665585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.055.7227971
GTATCAA42250.042.4184271
TCAACGC50550.034.8341834
ATCAACG49900.034.789383
TATCAAC51750.033.454932
CAACGCA52600.033.046315
AACGCAG56750.030.7158376
GTACATG47800.025.6644121
ACGCAGA66400.025.6520947
CGCAGAG70050.024.9992758
TACATGG50400.024.6366082
ACATGGG50600.023.3374943
GTGGTAT11550.021.8545041
GCAGAGT76400.021.3402069
CATGGGG38100.021.1883134
GTATACG2407.436938E-821.0349581
TGGTATC12250.020.192
AGAGTAC67950.018.61226810-11
CAGAGTA72200.018.52724810-11
GAGTACT49600.017.5779212-13
Produced by FastQC (version 0.11.5)