FastQCFastQC Report
Sat 18 Jun 2016
SRR1930941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677836
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.2215594980424492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48570.18137779908851775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.12334586584092529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.053.9302371
GTATCAA58900.041.041421
TCAACGC70750.033.4848184
CAACGCA71550.032.8309025
ATCAACG72150.032.7698863
TATCAAC74550.031.7786052
AACGCAG75250.031.4671176
ACGCAGA88650.026.6571397
CGCAGAG91550.025.7608978
GTACATG69100.023.9177911
ACATGGG68700.022.5290453
TACATGG72250.022.4734732
GCAGAGT105850.022.101369
AGAGTAC96650.020.0073110-11
CAGAGTA102200.019.24582510-11
GAGTACT72250.019.11252612-13
TATCACG2251.6771282E-618.9907422
GGACCGT3851.2732926E-1118.488156
GTGGTAT15200.018.434371
CATGGGG46350.018.334844
Produced by FastQC (version 0.11.5)