##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930941_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2677836 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25362568880245 31.0 31.0 34.0 30.0 34.0 2 31.37148130057255 31.0 31.0 34.0 30.0 34.0 3 31.452045606975183 31.0 31.0 34.0 28.0 34.0 4 35.31735401271773 37.0 35.0 37.0 33.0 37.0 5 35.10074478048693 37.0 35.0 37.0 32.0 37.0 6 35.14404952357052 37.0 35.0 37.0 32.0 37.0 7 35.02641498583184 37.0 35.0 37.0 32.0 37.0 8 35.00547905099491 36.0 35.0 37.0 32.0 37.0 9 36.5101331821665 38.0 35.0 39.0 32.0 39.0 10-11 36.440249701624744 38.0 35.0 39.0 32.0 39.0 12-13 36.340363636906815 38.0 35.0 39.0 32.0 39.0 14-15 37.352274373785406 39.0 36.0 41.0 32.0 41.0 16-17 37.31895045103583 39.0 36.0 41.0 32.0 41.0 18-19 37.312891827580174 39.0 36.0 40.5 32.0 41.0 20-21 37.28291463704275 39.0 36.0 40.0 31.5 41.0 22-23 37.15130127461129 39.0 36.0 40.0 31.0 41.0 24-25 37.02469998162695 39.0 36.0 40.0 31.0 41.0 26-27 36.74568532202868 39.0 35.5 40.0 30.0 41.0 28-29 36.5617836566541 38.0 35.0 40.0 30.0 41.0 30-31 36.32083742245604 38.0 35.0 40.0 30.0 41.0 32-33 36.07787295413162 38.0 34.5 40.0 30.0 41.0 34-35 36.26309583559262 38.0 35.0 40.0 30.0 41.0 36-37 36.30766036456303 38.0 35.0 40.0 30.0 41.0 38-39 36.273399117795115 38.0 35.0 40.0 30.0 41.0 40-41 36.17503909873495 38.0 35.0 40.0 30.0 41.0 42-43 36.03217093951982 38.0 35.0 40.0 29.5 41.0 44-45 35.840913521216386 38.0 35.0 40.0 28.5 41.0 46-47 35.611496372444016 38.0 34.0 40.0 28.0 41.0 48-49 35.4033583460675 38.0 34.0 40.0 27.0 41.0 50-51 35.137155150651495 38.0 34.0 40.0 26.5 41.0 52-53 34.92522768384621 38.0 33.5 40.0 26.0 41.0 54-55 34.756626806122554 38.0 33.0 40.0 26.0 41.0 56-57 34.50394385615848 37.5 33.0 40.0 26.0 41.0 58-59 34.26289866145649 37.0 33.0 40.0 25.0 41.0 60-61 33.94960539032263 37.0 32.5 40.0 24.5 41.0 62-63 33.648260946525475 36.5 32.0 39.0 23.5 41.0 64-65 33.34189659112806 36.0 32.0 39.0 23.0 41.0 66-67 33.000281383923436 35.5 31.0 39.0 22.5 40.0 68-69 32.62422493386451 35.0 31.0 38.5 22.0 40.0 70-71 32.217964804416695 35.0 31.0 38.0 21.5 40.0 72-73 31.785969342409317 35.0 30.5 37.5 20.5 39.5 74-75 31.25889916335429 34.0 30.0 37.0 19.0 39.0 76-77 29.89388651881594 33.0 28.5 35.5 18.0 38.0 78-79 30.64823312555362 34.0 29.5 36.0 18.5 38.5 80-81 30.639515452029173 34.0 30.0 36.0 18.5 37.5 82-83 30.35208280118723 34.0 30.0 35.0 18.0 37.0 84-85 30.02501030682984 34.0 29.5 35.0 17.0 36.5 86-87 29.67445784581281 34.0 29.0 35.0 12.0 36.0 88-89 29.317327872207258 34.0 29.0 35.0 7.5 36.0 90-91 28.96327127576147 34.0 29.0 35.0 4.5 35.5 92-93 28.597004073438406 33.5 29.0 35.0 2.0 35.0 94-95 28.163510013309256 33.0 28.5 35.0 2.0 35.0 96-97 27.691792178460517 33.0 27.0 35.0 2.0 35.0 98-99 27.18624142777974 33.0 27.0 35.0 2.0 35.0 100-101 25.959039687269872 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5154967929219225 1101 2 0.5305234136422179 1101 3 0.5826368776956947 1101 4 0.4412653072546604 1101 5 0.5026325878324585 1101 6 0.47342119863433396 1101 7 0.6222819063236074 1101 8 0.5172197254255622 1101 9 0.6826941999763605 1101 10-11 0.5993842716220854 1101 12-13 0.6697592079121648 1101 14-15 0.8202462744066992 1101 16-17 0.7875518128491379 1101 18-19 0.7231061814870969 1101 20-21 0.6874920612158206 1101 22-23 0.8238457647726989 1101 24-25 0.7989375712933224 1101 26-27 0.8280274085348225 1101 28-29 0.8270612947729177 1101 30-31 0.9049473655623004 1101 32-33 0.8260920073010354 1101 34-35 0.8024469007119777 1101 36-37 0.9132614677994226 1101 38-39 1.0553190689897676 1101 40-41 0.931733815079788 1101 42-43 1.0788559147846755 1101 44-45 1.1071140198474794 1101 46-47 1.057518516196481 1101 48-49 1.2469967683200238 1101 50-51 1.1871936652736252 1101 52-53 1.1578332056976635 1101 54-55 1.2182467557315704 1101 56-57 1.2255208136308653 1101 58-59 1.1497710876500165 1101 60-61 1.2446428286371756 1101 62-63 1.1930744339660677 1101 64-65 1.2334020335474918 1101 66-67 1.069606112911707 1101 68-69 1.1468796088356186 1101 70-71 1.1364035929055873 1101 72-73 1.168032159850089 1101 74-75 1.2076199040115654 1101 76-77 1.191138097443904 1101 78-79 1.164880622497531 1101 80-81 1.0953145610999293 1101 82-83 1.253019746923865 1101 84-85 1.3985862958198823 1101 86-87 1.2648711319377242 1101 88-89 1.1622089335560624 1101 90-91 1.2637319561180291 1101 92-93 1.424916952024148 1101 94-95 1.521399003205815 1101 96-97 1.5137276970641835 1101 98-99 1.5667443030917916 1101 100-101 1.7536344750019204 1102 1 0.3928158518083258 1102 2 0.4690851079570031 1102 3 0.4437920217977869 1102 4 0.26637962781771307 1102 5 0.3493322684675704 1102 6 0.4064024117037235 1102 7 0.40964953798081183 1102 8 0.46884348686300825 1102 9 0.6359246986769236 1102 10-11 0.5370615708553572 1102 12-13 0.5724623882003215 1102 14-15 0.6200329334879129 1102 16-17 0.5079086309232039 1102 18-19 0.5724844758803229 1102 20-21 0.6396955065639105 1102 22-23 0.661302180731461 1102 24-25 0.7221154459005845 1102 26-27 0.7056128203258965 1102 28-29 0.7300237166017851 1102 30-31 0.6946445076559726 1102 32-33 0.745841127000368 1102 34-35 0.8507871514629812 1102 36-37 0.8197819420627397 1102 38-39 0.8038881763627401 1102 40-41 0.9006649124278923 1102 42-43 0.8293896980733919 1102 44-45 1.0108981274574944 1102 46-47 1.0016701735752775 1102 48-49 0.9114059001733281 1102 50-51 0.9764555056946165 1102 52-53 0.7697578855785281 1102 54-55 0.831506217078072 1102 56-57 0.7464652889630514 1102 58-59 0.6478861452089646 1102 60-61 0.7226269078017395 1102 62-63 0.6634948233240152 1102 64-65 0.7138201386491403 1102 66-67 0.7920396647578585 1102 68-69 0.8016282759936146 1102 70-71 0.7287103900765644 1102 72-73 0.6603505937984444 1102 74-75 0.7879946944418208 1102 76-77 0.94384601864137 1102 78-79 1.0328206543047216 1102 80-81 1.004102051995229 1102 82-83 1.105160267475707 1102 84-85 1.1678930024825824 1102 86-87 1.0502273250737524 1102 88-89 1.1001411644124879 1102 90-91 0.9828643266157826 1102 92-93 1.1244129071665299 1102 94-95 1.0791628886826885 1102 96-97 1.1248668944713351 1102 98-99 1.152556916496497 1102 100-101 1.3777646258921301 1103 1 0.39771797331469116 1103 2 0.5298841092054438 1103 3 0.43119749559527065 1103 4 0.21184972622164366 1103 5 0.34210561155301633 1103 6 0.33621699532604765 1103 7 0.45594436305216846 1103 8 0.4698212209243877 1103 9 0.5055926642250412 1103 10-11 0.4074769186739502 1103 12-13 0.5016813358961727 1103 14-15 0.5982016367502467 1103 16-17 0.5446066556986366 1103 18-19 0.6691536226118941 1103 20-21 0.6534644906200313 1103 22-23 0.5781113460424976 1103 24-25 0.6678020245459635 1103 26-27 0.7251443823043928 1103 28-29 0.7899532239095706 1103 30-31 0.7335474782693083 1103 32-33 0.7853327977654558 1103 34-35 0.7382512640831465 1103 36-37 0.693586992147452 1103 38-39 0.8178553554360448 1103 40-41 0.8373866869773892 1103 42-43 0.8075066982877743 1103 44-45 0.9211143134539554 1103 46-47 0.7009807996025046 1103 48-49 0.7423629950505273 1103 50-51 0.7539323128842881 1103 52-53 0.7100833205356807 1103 54-55 0.8855077387450194 1103 56-57 0.8960493526544795 1103 58-59 0.9347777411517484 1103 60-61 0.8558391803398564 1103 62-63 0.7093838004864068 1103 64-65 0.7317587843655673 1103 66-67 0.5826242454797708 1103 68-69 0.7330933638257449 1103 70-71 0.6064675707051563 1103 72-73 0.6537141082605835 1103 74-75 0.727549627879398 1103 76-77 0.7874907472424013 1103 78-79 0.9694215782339874 1103 80-81 0.9942452652345715 1103 82-83 0.8725436308640653 1103 84-85 0.963068435548152 1103 86-87 0.9222442063290828 1103 88-89 0.8948731282455746 1103 90-91 1.1413856614791875 1103 92-93 1.206104353464717 1103 94-95 1.1572914485216117 1103 96-97 1.0482406654730738 1103 98-99 1.077034736729626 1103 100-101 1.3559255526797749 1104 1 0.45071600409050205 1104 2 0.5351344144557473 1104 3 0.40119005447671796 1104 4 0.286464411947442 1104 5 0.44664469386987804 1104 6 0.37376052175846297 1104 7 0.47262439084330765 1104 8 0.3945832901308961 1104 9 0.506418998384703 1104 10-11 0.5102866603725786 1104 12-13 0.5334678621271465 1104 14-15 0.5945380164199605 1104 16-17 0.5190355190163913 1104 18-19 0.6481008071146306 1104 20-21 0.6078603005713958 1104 22-23 0.6179388025366208 1104 24-25 0.6392965793738483 1104 26-27 0.7311283160661048 1104 28-29 0.6526726032956205 1104 30-31 0.6663488332928793 1104 32-33 0.7206487164147006 1104 34-35 0.7448646095853846 1104 36-37 0.7678105052598525 1104 38-39 0.6607326427577718 1104 40-41 0.7309381971955631 1104 42-43 0.8235782810260233 1104 44-45 0.8016150883991813 1104 46-47 0.8174745938740742 1104 48-49 0.8320895236659425 1104 50-51 0.7593896127860376 1104 52-53 0.7497204323950157 1104 54-55 0.7444810699405622 1104 56-57 0.8630455252062035 1104 58-59 0.8560270679566315 1104 60-61 0.7035895780902521 1104 62-63 0.5844562588921889 1104 64-65 0.6531974280264308 1104 66-67 0.6776263627041104 1104 68-69 0.6531387505377992 1104 70-71 0.6215304913236253 1104 72-73 0.5730661553904106 1104 74-75 0.7920630979484287 1104 76-77 0.9215922424550094 1104 78-79 1.0054160364506686 1104 80-81 0.8438662204110834 1104 82-83 0.8228579256228059 1104 84-85 0.9120610067629471 1104 86-87 0.9758765821836839 1104 88-89 0.977566360938809 1104 90-91 0.8341648987028698 1104 92-93 0.9776087749691378 1104 94-95 0.9171675806199637 1104 96-97 0.919211875333172 1104 98-99 1.2035035354206478 1104 100-101 1.436732811264811 1105 1 0.26206171805630163 1105 2 0.3324071562699764 1105 3 0.2930298740457964 1105 4 0.1889709301744844 1105 5 0.18621792994966313 1105 6 0.21726628818999671 1105 7 0.31736342182688304 1105 8 0.29306130411109876 1105 9 0.5359189743912296 1105 10-11 0.4062849488381204 1105 12-13 0.2763773907353624 1105 14-15 0.4287187192687796 1105 16-17 0.3737890260624752 1105 18-19 0.5511279837225942 1105 20-21 0.5385007663646988 1105 22-23 0.5177080652971497 1105 24-25 0.5479254323258473 1105 26-27 0.6286130002320149 1105 28-29 0.7218289927576862 1105 30-31 0.6876134493173183 1105 32-33 0.6197829519026499 1105 34-35 0.56948471291728 1105 36-37 0.6024250900927726 1105 38-39 0.4840886899129444 1105 40-41 0.4837398351500397 1105 42-43 0.4551812850089405 1105 44-45 0.36504823458341207 1105 46-47 0.40712500896117376 1105 48-49 0.4877727778208154 1105 50-51 0.5796517014979017 1105 52-53 0.5735195590325688 1105 54-55 0.5392928811279205 1105 56-57 0.550975208332396 1105 58-59 0.6278323369234684 1105 60-61 0.5111955092769662 1105 62-63 0.3910028782117365 1105 64-65 0.4871825840639872 1105 66-67 0.34427663383035423 1105 68-69 0.39186141797225815 1105 70-71 0.44591145970023405 1105 72-73 0.5581954002358138 1105 74-75 0.5679032694217838 1105 76-77 0.5452366111867164 1105 78-79 0.4337999160354187 1105 80-81 0.5293833597491826 1105 82-83 0.6969029853665525 1105 84-85 0.6260815931634411 1105 86-87 0.7461622644780981 1105 88-89 0.7821943021388016 1105 90-91 0.6032848980630447 1105 92-93 0.908398400191075 1105 94-95 0.6829760807079381 1105 96-97 0.5797905979206277 1105 98-99 0.6956280775451873 1105 100-101 1.0292304519065603 1106 1 0.0032557774591808197 1106 2 0.0770561484072374 1106 3 0.18841703224567397 1106 4 0.03765848885883827 1106 5 0.07084854527883522 1106 6 0.043353698684263975 1106 7 0.1099858604045636 1106 8 0.08637364642709855 1106 9 0.08181420183517218 1106 10-11 0.16693456436373566 1106 12-13 0.08447606398550533 1106 14-15 0.1958006043456706 1106 16-17 0.22834250675057888 1106 18-19 0.10409201746290364 1106 20-21 0.12801616312896158 1106 22-23 0.19147428228889396 1106 24-25 0.1628988184383644 1106 26-27 0.33405813509581606 1106 28-29 0.17539042744808597 1106 30-31 0.12676270210207718 1106 32-33 0.2639505969641931 1106 34-35 0.20529307591183255 1106 36-37 0.09952896391773436 1106 38-39 0.10464898146688739 1106 40-41 0.16092792054341487 1106 42-43 0.2588419900410628 1106 44-45 0.14263780992136788 1106 46-47 -0.04911889057275687 1106 48-49 0.0671140280315754 1106 50-51 -0.029576805138937345 1106 52-53 -0.03807623781398917 1106 54-55 0.12752089586031445 1106 56-57 0.12646823427185439 1106 58-59 0.12695912916498742 1106 60-61 -0.039365076182143355 1106 62-63 -0.22785373009154597 1106 64-65 -0.1416704741740915 1106 66-67 -0.08916409090887356 1106 68-69 -0.08621615597969878 1106 70-71 -0.1699817336104914 1106 72-73 -0.06164927087655414 1106 74-75 0.2909869689764406 1106 76-77 0.3121351922434421 1106 78-79 0.14236643992604314 1106 80-81 0.2769654095676586 1106 82-83 0.4167090620881808 1106 84-85 0.34809157201774 1106 86-87 0.41383246264753026 1106 88-89 0.4642026258428764 1106 90-91 0.4203790034079411 1106 92-93 0.40600094602868353 1106 94-95 0.5215007076417209 1106 96-97 0.5726467684598795 1106 98-99 0.4556351622068959 1106 100-101 0.751598144302303 1107 1 0.10913346320922201 1107 2 0.16659129324782995 1107 3 0.15147543996546986 1107 4 0.009830573602521042 1107 5 0.11725332787262488 1107 6 0.2035947361649093 1107 7 0.15857243064829163 1107 8 -0.07116890998315029 1107 9 0.13560794875177606 1107 10-11 0.21020099335142817 1107 12-13 0.16952982494226632 1107 14-15 0.14205020502611632 1107 16-17 0.20269302560517133 1107 18-19 0.2525553637781641 1107 20-21 0.31833248383460244 1107 22-23 0.23380376973398853 1107 24-25 0.38121793525032643 1107 26-27 0.35098816636073593 1107 28-29 0.3837598423772448 1107 30-31 0.1090029183382697 1107 32-33 0.26707125133653875 1107 34-35 0.22571308556008063 1107 36-37 0.23585529357111312 1107 38-39 0.28248063529819234 1107 40-41 0.36554677468632946 1107 42-43 0.4199374968452716 1107 44-45 0.3463856754558776 1107 46-47 0.376241142893079 1107 48-49 0.34630459797919144 1107 50-51 0.268891459700221 1107 52-53 0.09831790785082717 1107 54-55 0.38205370067589683 1107 56-57 0.3398562203549105 1107 58-59 0.3606894345755194 1107 60-61 0.191701898215193 1107 62-63 0.08779881395284406 1107 64-65 0.1417537524873822 1107 66-67 0.23889921794610558 1107 68-69 0.22819731749679306 1107 70-71 0.10366127545301396 1107 72-73 0.06842010018334932 1107 74-75 0.386618040434648 1107 76-77 0.35833286275102694 1107 78-79 0.3695126924828074 1107 80-81 0.4599004666697333 1107 82-83 0.3844686968058042 1107 84-85 0.6221024146304401 1107 86-87 0.5835962468107816 1107 88-89 0.505058667603624 1107 90-91 0.31116144119731004 1107 92-93 0.38975242841713964 1107 94-95 0.4674226551387122 1107 96-97 0.601072172586175 1107 98-99 0.47028913411368123 1107 100-101 0.7995267377684137 1108 1 0.18997870423648067 1108 2 0.27541512918441313 1108 3 0.1999871564283957 1108 4 -0.008861888110722305 1108 5 0.0684601724676952 1108 6 0.1968185713149353 1108 7 0.24579048167501583 1108 8 0.27410898700675546 1108 9 0.28540622216688405 1108 10-11 0.3312549490064782 1108 12-13 0.30955915604177875 1108 14-15 0.4431459979932413 1108 16-17 0.4459468481359181 1108 18-19 0.3990510310932507 1108 20-21 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-0.5623407072016455 2316 76-77 -1.0239661378048623 2316 78-79 -0.8071242839254751 2316 80-81 -0.7577725636903878 2316 82-83 -1.0005830198330656 2316 84-85 -0.9870979603064711 2316 86-87 -0.8888260037875604 2316 88-89 -0.9371284798754367 2316 90-91 -0.6796843532060812 2316 92-93 -0.8839064510983263 2316 94-95 -0.7334561323171833 2316 96-97 -0.6976106056684124 2316 98-99 -0.8452094017251248 2316 100-101 -1.1905096394851 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 17.0 4 43.0 5 82.0 6 191.0 7 480.0 8 1247.0 9 2186.0 10 3550.0 11 5295.0 12 7484.0 13 9536.0 14 11191.0 15 13808.0 16 16147.0 17 18562.0 18 16845.0 19 15130.0 20 15650.0 21 16457.0 22 18238.0 23 21564.0 24 25819.0 25 32898.0 26 41190.0 27 52372.0 28 66177.0 29 81689.0 30 99599.0 31 118942.0 32 142565.0 33 170623.0 34 209271.0 35 260401.0 36 333688.0 37 426844.0 38 360227.0 39 61806.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.47425093160835 21.415581548832265 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28.02180566696392 29.344758230153005 21.052558857226504 20-21 22.110371957057865 27.251370136184587 29.401184389185897 21.237073517571652 22-23 21.879660949857236 27.08933787531266 29.28273373181459 21.74826744301551 24-25 21.85667992684341 27.264255312395264 29.25546643862026 21.62359832214107 26-27 21.866525754052095 27.377036783233887 29.057475728517907 21.69896173419611 28-29 21.858648739232194 27.476643890161167 29.092166892440062 21.572540478166577 30-31 22.017604268824183 27.32465381906346 28.977000693893068 21.68074121821929 32-33 21.89595527723041 27.393350041059623 28.922594445063872 21.7881002366461 34-35 22.009123159667617 27.403503641401972 28.972203812440206 21.6151693864902 36-37 22.07108330071969 27.27997368004374 28.93772387647247 21.711219142764104 38-39 21.916278399314663 27.33093448745815 28.980562701201784 21.7722244120254 40-41 21.9937238892169 27.374519796861048 28.970808952065763 21.66094736185629 42-43 22.02284995304561 27.414397640273553 28.707982171337488 21.85477023534335 44-45 21.94902379069033 27.49958731730855 28.749717097611967 21.801671794389158 46-47 22.15000221278557 27.47947291261028 28.4667300687308 21.90379480587335 48-49 22.093108372971294 27.62370554174535 28.30540624925305 21.977779836030308 50-51 22.09214228818382 27.67250536048138 28.241084914290287 21.99426743704451 52-53 22.234828048476114 27.672231745609487 28.18455178721681 21.908388418697587 54-55 22.071197785716407 27.621174637780083 28.301288833020926 22.00633874348258 56-57 22.061558968256946 27.772701613211947 28.154487699840136 22.011251718690975 58-59 22.03932784245556 27.72108278187375 28.25290807230747 21.986681303363216 60-61 22.063665213884757 27.811080686282992 28.247793317751224 21.87746078208103 62-63 22.031964963839805 27.772870525673238 28.195066573115295 22.000097937371667 64-65 21.846220904206234 27.67054775688586 28.228403464664126 22.25482787424378 66-67 21.914423693039037 27.797121885824982 28.120661127364816 22.167793293771165 68-69 21.981630914732744 27.737012209608018 28.16716439559347 22.114192480065764 70-71 21.924951192371395 27.529259346338485 28.194624227049015 22.35116523424111 72-73 21.960294544381682 27.665527062652433 28.05083243037606 22.323345962589823 74-75 21.86535281894678 27.8249266968651 27.958262974926267 22.351457509261856 76-77 21.838057221462066 27.730176139067154 27.974939090146954 22.456827549323826 78-79 21.839697748255876 27.76160160708954 27.93785786004107 22.460842784613515 80-81 21.879131171269677 27.83733510791646 27.839034256325686 22.44449946448817 82-83 21.983515794096427 27.695796157793083 27.92269578868908 22.39799225942141 84-85 21.912973759408715 27.72503618593521 27.788781687900233 22.573208366755843 86-87 21.850049816344242 27.80394318397392 27.81249486525687 22.53351213442496 88-89 21.838221770779164 27.67512531123586 27.78831386951947 22.698339048465506 90-91 21.826408797666772 27.687374575866393 27.844414414128433 22.6418022123384 92-93 21.850314808845646 27.58572971147845 27.837397505508264 22.726557974167637 94-95 21.675389639610202 27.546992955024564 27.85523163647703 22.92238576888821 96-97 21.703046788526258 27.385732360010095 27.911604743531715 22.999616107931928 98-99 21.532479957697184 27.463743111975493 27.850305246475138 23.153471683852185 100-101 21.531639727003444 27.42010713128063 27.81635992644807 23.231893215267853 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 883.0 1 625.5 2 532.5 3 695.0 4 553.5 5 478.5 6 754.0 7 1416.0 8 2152.5 9 2429.0 10 2064.5 11 1582.5 12 1546.0 13 1662.0 14 1810.5 15 2035.5 16 2261.0 17 2463.0 18 2799.0 19 3191.5 20 3632.0 21 4376.0 22 5435.5 23 6890.0 24 8729.5 25 11363.0 26 14746.5 27 18839.5 28 23864.0 29 30572.0 30 38230.5 31 45661.0 32 54249.0 33 60575.0 34 64275.0 35 68529.5 36 74387.5 37 80283.0 38 83492.0 39 85479.0 40 87030.0 41 88361.0 42 90206.0 43 92608.0 44 95079.0 45 98182.0 46 100011.5 47 100432.5 48 100502.5 49 99386.0 50 96609.5 51 93313.0 52 91098.0 53 88561.0 54 84367.5 55 77109.0 56 68169.0 57 59324.0 58 51181.5 59 43611.5 60 36059.0 61 29523.0 62 23873.0 63 18241.5 64 13342.5 65 9722.5 66 7106.0 67 5421.0 68 4008.5 69 2837.0 70 2078.5 71 1530.5 72 1086.5 73 827.5 74 638.0 75 461.0 76 322.5 77 197.0 78 106.0 79 69.0 80 53.0 81 30.5 82 20.0 83 17.0 84 9.0 85 3.0 86 3.0 87 2.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01781289070727259 2 0.003024830497461383 3 0.003921076570783275 4 0.03484156610038852 5 0.0022406151833047282 6 1.1203075916523641E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.7343586388412136E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.468717277682427E-5 24-25 6.908563481856245E-4 26-27 0.001997881871780049 28-29 1.3070255235944248E-4 30-31 0.007095281413798306 32-33 0.02358247480428226 34-35 0.013611737238576222 36-37 7.468717277682428E-4 38-39 7.655435209624488E-4 40-41 0.0023526459424699645 42-43 0.008869101767247882 44-45 0.00873839921488844 46-47 0.008047542866702815 48-49 0.010008081152094452 50-51 0.1880249574656551 52-53 0.0516835235615624 54-55 0.018354372709904563 56-57 0.010810968259445313 58-59 0.02042694175446144 60-61 0.05513780530249052 62-63 0.09922190903401105 64-65 0.10349774967548424 66-67 0.10792296466251108 68-69 0.10652258017294562 70-71 0.09474067866740159 72-73 0.07119554744950773 74-75 0.23881223495389564 76-77 0.28760163057035604 78-79 0.007487389070876633 80-81 8.962460733218913E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.3070255235944248E-4 90-91 0.006404425065612681 92-93 0.01855976243504083 94-95 0.0015310870419248976 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2677836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.5936112485931 #Duplication Level Percentage of deduplicated Percentage of total 1 80.6197438045974 51.26900646470739 2 11.361592468389091 14.450493891993583 3 3.3645192219549926 6.418857823182742 4 1.4935904742384074 3.799312479332763 5 0.8478414384533904 2.695864941872644 6 0.5147144220252493 1.9639529314990793 7 0.35393013413156915 1.5755386751387792 8 0.24038190793957245 1.2229402883763412 9 0.18463018754905838 1.0567170334574685 >10 0.955545249833254 10.790675912630082 >50 0.046331711348283636 1.9927386896740646 >100 0.01600332723138619 1.7859506819575526 >500 9.405218466713009E-4 0.410641137402381 >1k 1.7634784623504483E-4 0.3455217144061219 >5k 5.878261541168161E-5 0.22178733436887602 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5933 0.2215594980424492 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4857 0.18137779908851775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3303 0.12334586584092529 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.7343586388412136E-5 2 0.0 0.0 0.0 0.0 3.7343586388412136E-5 3 0.0 0.0 0.0 0.0 3.7343586388412136E-5 4 0.0 0.0 0.0 0.0 3.7343586388412136E-5 5 0.0 0.0 0.0 0.0 3.7343586388412136E-5 6 0.0 0.0 0.0 0.0 3.7343586388412136E-5 7 0.0 0.0 0.0 0.0 3.7343586388412136E-5 8 0.0 0.0 0.0 0.0 3.7343586388412136E-5 9 0.0 0.0 0.0 0.0 3.7343586388412136E-5 10-11 0.0 0.0 0.0 0.0 3.7343586388412136E-5 12-13 0.0 0.0 0.0 1.8671793194206068E-5 1.3070255235944248E-4 14-15 0.0 0.0 0.0 3.7343586388412136E-5 1.4937434555364854E-4 16-17 0.0 0.0 0.0 3.7343586388412136E-5 1.4937434555364854E-4 18-19 0.0 0.0 0.0 3.7343586388412136E-5 1.4937434555364854E-4 20-21 0.0 0.0 0.0 1.3070255235944248E-4 1.8671793194206067E-4 22-23 0.0 0.0 0.0 2.80076897913091E-4 1.8671793194206067E-4 24-25 0.0 0.0 0.0 4.667948298551517E-4 1.8671793194206067E-4 26-27 0.0 0.0 0.0 9.149178665160973E-4 1.8671793194206067E-4 28-29 0.0 0.0 0.0 0.002109912630945286 1.8671793194206067E-4 30-31 0.0 0.0 0.0 0.007655435209624488 1.8671793194206067E-4 32-33 0.0 0.0 0.0 0.01941866492197431 1.8671793194206067E-4 34-35 0.0 0.0 0.0 0.03717554024966428 2.2406151833047282E-4 36-37 0.0 0.0 0.0 0.06523924542055601 2.2406151833047282E-4 38-39 0.0 0.0 0.0 0.10364712402103787 2.427333115246789E-4 40-41 0.0 0.0 0.0 0.14479975622106805 2.6140510471888497E-4 42-43 0.0 0.0 0.0 0.18761417801538258 2.987486911072971E-4 44-45 0.0 0.0 0.0 0.2412582398623366 3.7343586388412133E-4 46-47 0.0 0.0 0.0 0.3036967163037617 4.107794502725335E-4 48-49 0.0 0.0 0.0 0.3692160386222308 4.4812303666094563E-4 50-51 0.0 0.0 0.0 0.4388431554434252 4.8546662304935776E-4 52-53 0.0 0.0 0.0 0.511681820694023 5.601537958261821E-4 54-55 0.0 0.0 0.0 0.5902527264552422 5.974973822145942E-4 56-57 0.0 0.0 0.0 0.6797839748214602 6.161691754088003E-4 58-59 0.0 0.0 0.0 0.7812464990387762 6.348409686030063E-4 60-61 0.0 0.0 0.0 0.8932212428244299 6.721845549914184E-4 62-63 0.0 0.0 0.0 1.0062976224085418 7.095281413798305E-4 64-65 0.0 0.0 0.0 1.1220440684194253 7.281999345740366E-4 66-67 0.0 0.0 0.0 1.2602153380565502 7.468717277682427E-4 68-69 0.0 0.0 0.0 1.4169090265423274 7.468717277682427E-4 70-71 0.0 0.0 0.0 1.5875318727509824 7.655435209624488E-4 72-73 0.0 0.0 0.0 1.763457508226792 8.21558900545067E-4 74-75 0.0 0.0 0.0 1.9474120147761103 8.775742801276853E-4 76-77 0.0 1.8671793194206068E-5 0.0 2.151756119493501 8.962460733218913E-4 78-79 0.0 3.7343586388412136E-5 0.0 2.381605893714178 9.149178665160973E-4 80-81 0.0 3.7343586388412136E-5 0.0 2.6373161015088304 9.522614529045095E-4 82-83 0.0 3.7343586388412136E-5 0.0 2.9089720206913343 0.0010082768324871276 84-85 0.0 3.7343586388412136E-5 0.0 3.1897211031594166 0.0010082768324871276 86-87 0.0 3.7343586388412136E-5 0.0 3.4980297523821475 0.001064292212069746 88-89 3.7343586388412136E-5 3.7343586388412136E-5 0.0 3.8308357942756763 0.0011203075916523641 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3135 0.0 53.930237 1 GTATCAA 5890 0.0 41.04142 1 TCAACGC 7075 0.0 33.484818 4 CAACGCA 7155 0.0 32.830902 5 ATCAACG 7215 0.0 32.769886 3 TATCAAC 7455 0.0 31.778605 2 AACGCAG 7525 0.0 31.467117 6 ACGCAGA 8865 0.0 26.657139 7 CGCAGAG 9155 0.0 25.760897 8 GTACATG 6910 0.0 23.917791 1 ACATGGG 6870 0.0 22.529045 3 TACATGG 7225 0.0 22.473473 2 GCAGAGT 10585 0.0 22.10136 9 AGAGTAC 9665 0.0 20.00731 10-11 CAGAGTA 10220 0.0 19.245825 10-11 GAGTACT 7225 0.0 19.112526 12-13 TATCACG 225 1.6771282E-6 18.990742 2 GGACCGT 385 1.2732926E-11 18.48815 6 GTGGTAT 1520 0.0 18.43437 1 CATGGGG 4635 0.0 18.33484 4 >>END_MODULE