FastQCFastQC Report
Sat 18 Jun 2016
SRR1930940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267505
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5040.18840769331414367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2000.050.4152761
GTACTAG404.0084773E-450.4152761
GTATCAA3350.045.148011
GTGATAC500.002599117634.4809159
TATCAAC5000.034.282391
TCAACGC5000.034.2477953
CAACGCA5000.034.2305264
GTCTAGG600.00295626333.5847432
GTAATAC600.002959922733.576273
ATCAACG5150.033.2586752
AACGCAG5400.031.3467065
TAAGGTG953.0747044E-530.5453645
TACCCTA804.0322435E-430.2271845
CTTAGAC700.00629302728.779663
CTATAGC906.3363876E-427.9802233
ACGCAGA6350.027.0789416
GTACATG5250.025.9278581
CGCAGAG6800.025.2984247
GTGTAGC1000.00116539625.2076381
TACATGG5200.025.1885572
Produced by FastQC (version 0.11.5)