##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930940_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267505 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.619543559933458 30.0 10.0 31.0 2.0 33.0 2 21.57105474664025 28.0 10.0 31.0 2.0 33.0 3 22.000276630343357 28.0 10.0 31.0 2.0 33.0 4 23.919220201491562 32.0 2.0 35.0 2.0 37.0 5 23.898218724883648 32.0 10.0 35.0 2.0 37.0 6 24.64336367544532 32.0 11.0 35.0 2.0 37.0 7 24.709919440758117 32.0 11.0 35.0 2.0 37.0 8 25.48270873441618 32.0 17.0 35.0 2.0 37.0 9 26.655359712902563 33.0 17.0 37.0 2.0 39.0 10-11 26.887084353563488 33.5 17.0 37.0 2.0 39.0 12-13 26.834795611296983 33.0 17.0 37.0 2.0 39.0 14-15 27.07764340853442 33.5 17.0 38.0 2.0 40.0 16-17 26.160357750322422 33.0 10.5 38.0 2.0 40.0 18-19 25.537558176482683 32.0 10.0 38.0 2.0 40.0 20-21 25.001854170950075 32.0 10.0 38.0 2.0 40.0 22-23 24.98074428515355 32.0 10.0 38.0 2.0 40.0 24-25 24.68161903515822 32.0 8.5 38.0 2.0 40.0 26-27 24.33703295265509 31.5 5.0 37.5 2.0 39.5 28-29 24.256118577222857 31.0 5.0 37.0 2.0 39.5 30-31 24.188546008485822 31.0 2.0 37.0 2.0 39.0 32-33 23.906026055587745 30.0 2.0 37.0 2.0 39.0 34-35 21.021644455243827 25.0 2.0 36.0 2.0 39.0 36-37 19.43029289172165 18.0 2.0 35.0 2.0 38.5 38-39 18.52897142109493 17.5 2.0 34.0 2.0 38.0 40-41 19.851212126876135 23.5 2.0 34.5 2.0 38.0 42-43 19.76306050354199 23.0 2.0 35.0 2.0 38.0 44-45 17.83279004130764 16.5 2.0 34.0 2.0 38.0 46-47 18.68387132950786 18.5 2.0 33.0 2.0 38.0 48-49 18.119287489953457 17.0 2.0 33.0 2.0 38.0 50-51 16.204884020859424 11.0 2.0 31.5 2.0 37.5 52-53 19.118666940804843 22.0 2.0 33.5 2.0 38.0 54-55 20.71405768116484 26.5 2.0 35.5 2.0 38.5 56-57 20.94630193828153 28.0 2.0 36.0 2.0 39.0 58-59 20.583417132390046 26.5 2.0 36.0 2.0 39.0 60-61 20.26283807779294 26.0 2.0 35.0 2.0 39.0 62-63 19.79191230070466 25.5 2.0 35.0 2.0 38.5 64-65 19.30680361114746 24.0 2.0 34.0 2.0 38.0 66-67 18.88543017887516 23.0 2.0 34.0 2.0 38.0 68-69 18.440298312181078 22.0 2.0 34.0 2.0 37.0 70-71 17.848367320237003 20.5 2.0 33.0 2.0 36.5 72-73 17.36086802115848 18.0 2.0 32.5 2.0 36.0 74-75 16.820805218594046 14.5 2.0 32.0 2.0 35.5 76-77 16.243169286555393 9.5 2.0 31.5 2.0 35.0 78-79 15.73990205790546 7.0 2.0 31.0 2.0 35.0 80-81 15.239434776920056 2.0 2.0 31.0 2.0 34.0 82-83 14.763017513691333 2.0 2.0 30.5 2.0 34.0 84-85 14.219943552456964 2.0 2.0 30.0 2.0 34.0 86-87 13.681925571484646 2.0 2.0 29.5 2.0 34.0 88-89 13.11701276611652 2.0 2.0 29.0 2.0 34.0 90-91 12.530406908282089 2.0 2.0 29.0 2.0 34.0 92-93 11.90289153473767 2.0 2.0 28.0 2.0 34.0 94-95 11.235803069101511 2.0 2.0 26.0 2.0 33.0 96-97 10.519666922113606 2.0 2.0 25.0 2.0 33.0 98-99 9.719739817947328 2.0 2.0 22.0 2.0 32.0 100-101 8.676407917609016 2.0 2.0 10.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.721818862444412 1101 2 8.225748534991197 1101 3 7.360667996038362 1101 4 9.06275269955814 1101 5 9.097139209276836 1101 6 8.938706226579743 1101 7 8.487198775606732 1101 8 7.2629565543010735 1101 9 6.952875532549509 1101 10-11 7.232688343529176 1101 12-13 6.959535975225688 1101 14-15 7.509973540528986 1101 16-17 7.641315257679874 1101 18-19 8.185697585715491 1101 20-21 9.111840473527412 1101 22-23 8.832511856887063 1101 24-25 8.414546245671968 1101 26-27 9.379907663779175 1101 28-29 8.387568774039384 1101 30-31 8.254719535876763 1101 32-33 8.597609589981342 1101 34-35 10.846513409193168 1101 36-37 5.127523938341906 1101 38-39 8.341342014963246 1101 40-41 9.302272148447475 1101 42-43 9.56957096389359 1101 44-45 4.602410629509166 1101 46-47 5.621135160226213 1101 48-49 8.453526189365373 1101 50-51 -3.995942077517711 1101 52-53 4.6590259579694475 1101 54-55 7.239651137645545 1101 56-57 8.05300922732092 1101 58-59 8.358441248163917 1101 60-61 8.230490375649836 1101 62-63 7.882061426951893 1101 64-65 7.802804464545556 1101 66-67 7.15262599409991 1101 68-69 7.7345897012109255 1101 70-71 7.263627918518512 1101 72-73 7.738627491691361 1101 74-75 7.109374360416396 1101 76-77 7.227141939371673 1101 78-79 6.99422109120564 1101 80-81 6.6274490721710375 1101 82-83 6.718426608803814 1101 84-85 6.804116518456434 1101 86-87 6.343692449690922 1101 88-89 6.48381774943611 1101 90-91 6.42278704096938 1101 92-93 7.1130764161720155 1101 94-95 6.582284198373868 1101 96-97 6.0207857002460745 1101 98-99 5.767177653400228 1101 100-101 5.513440834018931 1102 1 6.129435888738481 1102 2 7.537342097071139 1102 3 7.11225978945895 1102 4 9.130344964621223 1102 5 8.910118221185815 1102 6 8.099536317370923 1102 7 7.76543836999419 1102 8 7.06094617816618 1102 9 6.932300128705624 1102 10-11 6.823354243776791 1102 12-13 6.1447661957185495 1102 14-15 7.096040926450453 1102 16-17 7.531783657632712 1102 18-19 7.321324280680702 1102 20-21 8.238476483431906 1102 22-23 7.882071165930807 1102 24-25 8.19777493332673 1102 26-27 8.207544440102726 1102 28-29 7.972899218562436 1102 30-31 7.577028225889734 1102 32-33 7.835707986396859 1102 34-35 10.598178896753598 1102 36-37 1.2415214032634942 1102 38-39 3.8947628969347114 1102 40-41 9.268358105292187 1102 42-43 9.951623493076468 1102 44-45 1.348689075447144 1102 46-47 1.5801833267160141 1102 48-49 7.0354151175578 1102 50-51 -4.1601399904996725 1102 52-53 5.291505913163412 1102 54-55 8.1581896354642 1102 56-57 8.62924581498747 1102 58-59 9.454199413474612 1102 60-61 9.230726196897333 1102 62-63 8.908060719488152 1102 64-65 8.618215383069312 1102 66-67 8.911697742714463 1102 68-69 9.276551144436961 1102 70-71 8.973840452417814 1102 72-73 8.630982461624154 1102 74-75 8.708301373740294 1102 76-77 8.244614819928213 1102 78-79 8.047155591854152 1102 80-81 7.906300327919178 1102 82-83 7.301465165577781 1102 84-85 7.37803174071796 1102 86-87 7.403731065420198 1102 88-89 6.77516016188747 1102 90-91 6.699574860801947 1102 92-93 6.52748804215952 1102 94-95 6.772429817994192 1102 96-97 6.547750386014259 1102 98-99 6.0643713805550465 1102 100-101 5.254892313797258 1103 1 6.309634824662609 1103 2 7.155575962442409 1103 3 6.982315306461032 1103 4 8.309329466124808 1103 5 8.171366197128446 1103 6 7.058823849177543 1103 7 7.764339595994652 1103 8 6.590844396995699 1103 9 6.596190945268194 1103 10-11 5.888456603249374 1103 12-13 5.700268740247818 1103 14-15 6.2543077550417046 1103 16-17 6.034400474657925 1103 18-19 7.164720070641376 1103 20-21 7.720433457755288 1103 22-23 7.456513104399466 1103 24-25 7.66338041563068 1103 26-27 7.302198037141814 1103 28-29 7.838024710119228 1103 30-31 7.795857742429174 1103 32-33 7.613307454358921 1103 34-35 9.295640150512561 1103 36-37 -3.3111474609511724 1103 38-39 -1.7472536425054912 1103 40-41 5.944711332217935 1103 42-43 8.115268531244407 1103 44-45 0.3958062394536199 1103 46-47 0.32612075442824917 1103 48-49 6.513555297988059 1103 50-51 -5.295419310416396 1103 52-53 3.7083026978037097 1103 54-55 6.564880590819278 1103 56-57 7.331434688454966 1103 58-59 7.583305358420247 1103 60-61 7.811630661389582 1103 62-63 7.7919954870107055 1103 64-65 7.500646565118817 1103 66-67 7.19568744893699 1103 68-69 6.63611047823294 1103 70-71 6.4340098949345865 1103 72-73 6.381980032755312 1103 74-75 6.654403617552465 1103 76-77 6.25171347826733 1103 78-79 6.081665769971197 1103 80-81 5.737363825490311 1103 82-83 6.093623389033716 1103 84-85 5.680107845274984 1103 86-87 5.49595579361821 1103 88-89 5.297106726772967 1103 90-91 4.951063988722373 1103 92-93 4.960144762029978 1103 94-95 4.784024775199841 1103 96-97 4.512112518792426 1103 98-99 3.9998906958453535 1103 100-101 3.2166665446554585 1104 1 6.287868036301063 1104 2 7.2624738493768035 1104 3 6.754552279577528 1104 4 8.636273818376168 1104 5 8.461995691541542 1104 6 7.6538841434578835 1104 7 7.30792848857126 1104 8 6.408772265122703 1104 9 6.5060814198782175 1104 10-11 6.459800225331463 1104 12-13 6.2072661957185495 1104 14-15 6.296057395488656 1104 16-17 6.996951641822431 1104 18-19 7.626330868295984 1104 20-21 8.172765020981313 1104 22-23 7.441771429435416 1104 24-25 7.23063066455202 1104 26-27 7.583285546822086 1104 28-29 6.940372868101306 1104 30-31 6.845144167918718 1104 32-33 6.70206174133757 1104 34-35 8.752493784764138 1104 36-37 -3.4494370947602224 1104 38-39 -2.557229856688476 1104 40-41 4.878041899758593 1104 42-43 7.552166971337336 1104 44-45 -0.31278984418394984 1104 46-47 -0.3948660803986108 1104 48-49 5.900863075397066 1104 50-51 -5.507389661118907 1104 52-53 3.2835184296324513 1104 54-55 6.330949846267888 1104 56-57 6.406996144368232 1104 58-59 6.584798886465386 1104 60-61 6.7409370005863956 1104 62-63 6.762968492874183 1104 64-65 6.061397201251133 1104 66-67 6.225350575389033 1104 68-69 5.741307402671481 1104 70-71 5.789469569677369 1104 72-73 6.21950354199306 1104 74-75 6.0968059850709935 1104 76-77 5.948748548518463 1104 78-79 6.057119359970089 1104 80-81 6.151112580883607 1104 82-83 5.525114704444711 1104 84-85 5.1611345075163815 1104 86-87 5.1212047149590685 1104 88-89 4.867551466235298 1104 90-91 4.504499103226189 1104 92-93 4.409240347824868 1104 94-95 3.8259541921707427 1104 96-97 3.917397948596607 1104 98-99 3.960616439843582 1104 100-101 3.9363811148512777 1105 1 6.121915428663279 1105 2 6.902720331974923 1105 3 6.332067354157026 1105 4 8.796513953782913 1105 5 9.029699066131624 1105 6 7.54965797187214 1105 7 7.511567544955479 1105 8 6.976396299820681 1105 9 6.141435275796976 1105 10-11 5.862463401042881 1105 12-13 5.39948030764069 1105 14-15 6.058341855448457 1105 16-17 6.550294495942818 1105 18-19 7.387570686343171 1105 20-21 8.025470511301844 1105 22-23 7.984191825077016 1105 24-25 8.08301879530416 1105 26-27 7.475806439660342 1105 28-29 7.898759094696036 1105 30-31 7.581894405938396 1105 32-33 6.972002500884802 1105 34-35 9.031849544840306 1105 36-37 -3.5869595743963174 1105 38-39 -1.6288076626759924 1105 40-41 5.500026983858874 1105 42-43 7.906459259499826 1105 44-45 -3.0207397013721433 1105 46-47 -3.862072258331544 1105 48-49 5.118029583383947 1105 50-51 -9.805337402576646 1105 52-53 0.7476964683503162 1105 54-55 5.1444482065767865 1105 56-57 6.443598281255991 1105 58-59 6.789495808077575 1105 60-61 6.4680077717701465 1105 62-63 6.468445590091999 1105 64-65 6.499657541883732 1105 66-67 6.1342287093147725 1105 68-69 5.9370521397919696 1105 70-71 6.802957351333983 1105 72-73 6.71380429307737 1105 74-75 6.759783346755118 1105 76-77 6.971624884073314 1105 78-79 6.633986492097458 1105 80-81 6.431142730042604 1105 82-83 6.242241542485544 1105 84-85 5.824310218930746 1105 86-87 6.212802620385917 1105 88-89 6.270598118091851 1105 90-91 5.535521001036409 1105 92-93 4.840333651537977 1105 94-95 4.671373635733632 1105 96-97 4.70353726944713 1105 98-99 4.400027208911604 1105 100-101 3.7259717210080527 1106 1 5.266333029156748 1106 2 6.270536719350268 1106 3 6.106818048101715 1106 4 7.049572450750119 1106 5 7.01409816143336 1106 6 6.3518837352113025 1106 7 5.990363679426537 1106 8 5.3379414833305 1106 9 4.705560905487527 1106 10-11 4.9252268430085415 1106 12-13 5.041186383511981 1106 14-15 4.875410324657878 1106 16-17 5.870318655498696 1106 18-19 5.545982838085738 1106 20-21 5.8133804527020665 1106 22-23 5.660106804001657 1106 24-25 6.067893371226855 1106 26-27 6.202289425164604 1106 28-29 6.048149965468117 1106 30-31 5.76599536203997 1106 32-33 6.228980500267962 1106 34-35 8.223712400808228 1106 36-37 -0.4222376663054348 1106 38-39 0.8739295636013793 1106 40-41 5.977705096329316 1106 42-43 7.0880671550483 1106 44-45 -3.7145044798238764 1106 46-47 -4.799591534572933 1106 48-49 3.1103190868279675 1106 50-51 -9.929746264507354 1106 52-53 -1.0130982268280526 1106 54-55 2.730266033244824 1106 56-57 4.211165362576029 1106 58-59 4.617024850947935 1106 60-61 5.038931489257553 1106 62-63 4.700634343047689 1106 64-65 4.488040393739393 1106 66-67 4.511863128971399 1106 68-69 4.183081234065643 1106 70-71 4.2743899615940855 1106 72-73 4.213328812456417 1106 74-75 3.7477806737829766 1106 76-77 4.06293001412369 1106 78-79 3.8661109909151836 1106 80-81 3.387334258777054 1106 82-83 3.116766061864592 1106 84-85 2.9046535930440402 1106 86-87 3.1339113897906667 1106 88-89 3.072695373155078 1106 90-91 2.9305554412543575 1106 92-93 2.4955844996925958 1106 94-95 2.7200397198294493 1106 96-97 2.5525785977086723 1106 98-99 2.296552345130376 1106 100-101 2.1304258178518882 1107 1 5.770797963090246 1107 2 7.11227961659629 1107 3 6.475193247753232 1107 4 7.681657054593106 1107 5 8.423746774762822 1107 6 6.580635973728313 1107 7 5.8286217501878745 1107 8 5.630533807032162 1107 9 6.091469138390099 1107 10-11 5.413501853898623 1107 12-13 4.918606401904121 1107 14-15 5.343326930824087 1107 16-17 6.298087156539307 1107 18-19 6.814998132601975 1107 20-21 7.4869925721541755 1107 22-23 7.262479632035149 1107 24-25 7.43971104704119 1107 26-27 7.077682678153327 1107 28-29 6.633727865860159 1107 30-31 6.9120797722814835 1107 32-33 6.627394178211912 1107 34-35 8.53745568525719 1107 36-37 -0.7557646011101404 1107 38-39 0.40614605844674045 1107 40-41 5.7983877835177395 1107 42-43 7.167903561804991 1107 44-45 -3.5607728114631954 1107 46-47 -4.862705614239939 1107 48-49 3.8131408282732018 1107 50-51 -9.826006126082302 1107 52-53 0.028755991264006298 1107 54-55 4.4916401196878795 1107 56-57 5.209513700023397 1107 58-59 5.658159113568331 1107 60-61 6.169182929168496 1107 62-63 5.463980744480342 1107 64-65 6.0051257235878985 1107 66-67 5.798393306600747 1107 68-69 5.621013812353237 1107 70-71 5.095423551449368 1107 72-73 5.6575171382876945 1107 74-75 5.811862760806836 1107 76-77 5.644587484720464 1107 78-79 5.501408169173738 1107 80-81 5.2904185722178365 1107 82-83 5.0948890955996475 1107 84-85 5.204687473301528 1107 86-87 4.513013320965344 1107 88-89 4.731775282162385 1107 90-91 4.810341027624817 1107 92-93 4.29189760167217 1107 94-95 3.8943838948451788 1107 96-97 4.129541232624696 1107 98-99 3.2939871256347075 1107 100-101 3.3226055284176894 1108 1 6.519752094272079 1108 2 5.911024574014487 1108 3 6.269045033200715 1108 4 8.360911499535582 1108 5 8.722865256758933 1108 6 7.708231969544837 1108 7 7.938363271179956 1108 8 6.11167081584734 1108 9 5.053182869153581 1108 10-11 6.144582834027119 1108 12-13 5.63661402180551 1108 14-15 6.453666091140828 1108 16-17 7.094777728778961 1108 18-19 7.706041013223523 1108 20-21 7.939069368807399 1108 22-23 7.8656844729136814 1108 24-25 7.638239360204192 1108 26-27 7.467343517836582 1108 28-29 7.837111998536088 1108 30-31 7.825216631686832 1108 32-33 8.390467538439015 1108 34-35 10.36300103114095 1108 36-37 -2.9132052107022517 1108 38-39 -1.5825921755290544 1108 40-41 6.580940450483226 1108 42-43 8.838398855395312 1108 44-45 -2.379818829691196 1108 46-47 -3.086895065905857 1108 48-49 6.603761626121706 1108 50-51 -9.581665023437747 1108 52-53 1.5461995890527405 1108 54-55 6.483123759311368 1108 56-57 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-16.310042140327056 2313 70-71 -15.740412266408075 2313 72-73 -15.146911900111673 2313 74-75 -14.619594307813923 2313 76-77 -14.127818133297424 2313 78-79 -13.683608055068035 2313 80-81 -13.127652000240465 2313 82-83 -12.614990330146007 2313 84-85 -12.080697538133268 2313 86-87 -11.573178711794455 2313 88-89 -10.990083740551555 2313 90-91 -10.382260478655343 2313 92-93 -9.80646560581852 2313 94-95 -9.246509575945199 2313 96-97 -8.505814443465201 2313 98-99 -7.675941152440415 2313 100-101 -6.638228792738483 2314 1 -20.476624717322125 2314 2 -19.451294266565224 2314 3 -19.865012937813777 2314 4 -21.816624732348473 2314 5 -21.813366627299036 2314 6 -22.56713034929574 2314 7 -22.59062223606642 2314 8 -23.355720488500484 2314 9 -24.537396840991345 2314 10-11 -24.705054847132303 2314 12-13 -24.62606713761478 2314 14-15 -24.886913619004094 2314 16-17 -24.06101937267032 2314 18-19 -23.413524204167782 2314 20-21 -22.897887152931734 2314 22-23 -22.86982277346313 2314 24-25 -22.546543248491457 2314 26-27 -22.192076772018492 2314 28-29 -22.108540175376955 2314 30-31 -22.089638440776934 2314 32-33 -21.779822316633446 2314 34-35 -19.019245345670644 2314 36-37 -17.358857384615295 2314 38-39 -16.50107043639862 2314 40-41 -17.83391462198054 2314 42-43 -17.735876506923532 2314 44-45 -15.82365940940134 2314 46-47 -16.637619703587017 2314 48-49 -16.100948518805833 2314 50-51 -14.188549081408762 2314 52-53 -17.058872874715377 2314 54-55 -18.61832551605095 2314 56-57 -18.857496609254955 2314 58-59 -18.458679374404177 2314 60-61 -18.11775865158752 2314 62-63 -17.637393825966395 2314 64-65 -17.230269465415535 2314 66-67 -16.790953772437053 2314 68-69 -16.325721582835765 2314 70-71 -15.808356517279154 2314 72-73 -15.249699356557667 2314 74-75 -14.731092565653643 2314 76-77 -14.18530942249603 2314 78-79 -13.723677741479184 2314 80-81 -13.213017853899002 2314 82-83 -12.721262107149492 2314 84-85 -12.193937956251736 2314 86-87 -11.677708328519193 2314 88-89 -11.09983983811253 2314 90-91 -10.49550089677381 2314 92-93 -9.900542260870784 2314 94-95 -9.246509575945199 2314 96-97 -8.533689007925132 2314 98-99 -7.738658922475258 2314 100-101 -6.737531928626984 2315 1 -20.476624717322125 2315 2 -19.451294266565224 2315 3 -19.865012937813777 2315 4 -17.152313778284867 2315 5 -21.813366627299036 2315 6 -22.56713034929574 2315 7 -22.59062223606642 2315 8 -23.355720488500484 2315 9 -20.070965745584985 2315 10-11 -18.332263327697675 2315 12-13 -15.09249823302114 2315 14-15 -15.061825279781479 2315 16-17 -20.296001704825798 2315 18-19 -23.413524204167782 2315 20-21 -22.897887152931734 2315 22-23 -22.86982277346313 2315 24-25 -22.546543248491457 2315 26-27 -22.192076772018492 2315 28-29 -22.108540175376955 2315 30-31 -22.089638440776934 2315 32-33 -21.779822316633446 2315 34-35 -19.019245345670644 2315 36-37 -17.358857384615295 2315 38-39 -16.50107043639862 2315 40-41 -17.83391462198054 2315 42-43 -17.735876506923532 2315 44-45 -15.82365940940134 2315 46-47 -16.637619703587017 2315 48-49 -16.100948518805833 2315 50-51 -14.188549081408762 2315 52-53 -17.058872874715377 2315 54-55 -18.61832551605095 2315 56-57 -18.857496609254955 2315 58-59 -18.458679374404177 2315 60-61 -18.11775865158752 2315 62-63 -17.637393825966395 2315 64-65 -17.230269465415535 2315 66-67 -16.790953772437053 2315 68-69 -16.325721582835765 2315 70-71 -15.808356517279154 2315 72-73 -15.249699356557667 2315 74-75 -14.731092565653643 2315 76-77 -14.18530942249603 2315 78-79 -13.723677741479184 2315 80-81 -13.213017853899002 2315 82-83 -12.721262107149492 2315 84-85 -12.193937956251736 2315 86-87 -11.677708328519193 2315 88-89 -11.09983983811253 2315 90-91 -10.49550089677381 2315 92-93 -9.900542260870784 2315 94-95 -9.246509575945199 2315 96-97 -8.533689007925132 2315 98-99 -7.738658922475258 2315 100-101 -6.737531928626984 2316 1 -20.476624717322125 2316 2 -19.451294266565224 2316 3 -19.865012937813777 2316 4 -21.816624732348473 2316 5 -21.813366627299036 2316 6 -22.56713034929574 2316 7 -22.59062223606642 2316 8 -23.355720488500484 2316 9 -24.537396840991345 2316 10-11 -24.705054847132303 2316 12-13 -24.62606713761478 2316 14-15 -24.886913619004094 2316 16-17 -24.06101937267032 2316 18-19 -23.413524204167782 2316 20-21 -22.897887152931734 2316 22-23 -22.86982277346313 2316 24-25 -22.546543248491457 2316 26-27 -22.192076772018492 2316 28-29 -22.108540175376955 2316 30-31 -22.089638440776934 2316 32-33 -21.779822316633446 2316 34-35 -19.019245345670644 2316 36-37 -17.358857384615295 2316 38-39 -16.50107043639862 2316 40-41 -17.83391462198054 2316 42-43 -17.735876506923532 2316 44-45 -15.82365940940134 2316 46-47 -16.637619703587017 2316 48-49 -16.100948518805833 2316 50-51 -14.188549081408762 2316 52-53 -17.058872874715377 2316 54-55 -18.61832551605095 2316 56-57 -18.857496609254955 2316 58-59 -18.458679374404177 2316 60-61 -18.11775865158752 2316 62-63 -17.637393825966395 2316 64-65 -17.230269465415535 2316 66-67 -16.790953772437053 2316 68-69 -16.325721582835765 2316 70-71 -15.808356517279154 2316 72-73 -15.249699356557667 2316 74-75 -14.731092565653643 2316 76-77 -14.18530942249603 2316 78-79 -13.723677741479184 2316 80-81 -13.213017853899002 2316 82-83 -12.721262107149492 2316 84-85 -12.193937956251736 2316 86-87 -11.677708328519193 2316 88-89 -11.09983983811253 2316 90-91 -10.49550089677381 2316 92-93 -9.900542260870784 2316 94-95 -9.246509575945199 2316 96-97 -8.533689007925132 2316 98-99 -7.738658922475258 2316 100-101 -6.737531928626984 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 45882.0 3 2859.0 4 5810.0 5 3480.0 6 3050.0 7 3585.0 8 4817.0 9 5583.0 10 5564.0 11 5185.0 12 4603.0 13 4318.0 14 4203.0 15 4474.0 16 4873.0 17 5134.0 18 5437.0 19 5762.0 20 6021.0 21 6273.0 22 6753.0 23 7015.0 24 7356.0 25 7719.0 26 8439.0 27 8975.0 28 9426.0 29 9939.0 30 10250.0 31 10375.0 32 10202.0 33 9775.0 34 9176.0 35 7520.0 36 5332.0 37 2103.0 38 235.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.76319295907991 22.2671727620183 15.00184087801115 23.96779340089063 2 14.874768732102137 21.219755895837416 41.04497234346689 22.860503028593556 3 18.059079006485167 26.32732619617062 30.8596816867787 24.753913110565513 4 12.09975623112972 17.15299283232288 39.55307425015629 31.194176686391106 5 12.68516637156154 38.09081497392432 36.10199994194653 13.122018712567607 6 30.294171632202637 38.177398342682416 18.19557421890739 13.332855806207554 7 25.79886201614719 32.97946067157822 23.552910952101385 17.668766360173212 8 24.64318924406453 36.29317772716028 21.331439442411213 17.732193586363984 9 25.60464963703655 16.196930344207445 21.09356005202283 37.10485996673317 10-11 24.257700388349125 26.53812490862079 29.676562659600673 19.52761204342941 12-13 24.634379035792268 24.36212667103615 29.523092973019093 21.48040132015249 14-15 21.835946743467822 25.876704211052164 27.042474572089066 25.24487447339095 16-17 21.7059533642544 28.900819620052097 27.667153779359126 21.72607323633437 18-19 21.721860412009857 28.07481347134331 29.31606891130502 20.88725720534181 20-21 22.17065861656538 27.449124154275957 29.31799224118527 21.062224987973398 22-23 21.858687827859843 27.270180217847646 29.1236524610232 21.74747949326931 24-25 21.730176448526784 27.41310170811761 29.22663127783708 21.63009056551853 26-27 21.856420975509895 27.51123226272182 28.963184036718992 21.669162725049294 28-29 21.688682459386147 27.542166388504345 29.136963675020162 21.632187477089342 30-31 21.962139351066124 27.624243190072313 28.869997538121 21.543619920740568 32-33 21.891801111213326 27.65367969072584 28.838275030647154 21.61624416741368 34-35 22.1394225185254 27.562545006852655 28.865943459778393 21.43208901484355 36-37 22.51978299181036 27.65496882605283 28.309794493836787 21.51545368830002 38-39 22.360389372758547 27.600819732123256 28.432506282173264 21.606284612944936 40-41 22.425879595763174 27.532499696157366 28.467417750372064 21.574202957707396 42-43 22.42235498204534 27.65567387832081 28.199240564924366 21.722730574709484 44-45 22.722193086073627 27.746871676232583 27.982384339020598 21.548550898673188 46-47 22.029141955211877 27.622281381576403 28.332121952373374 22.01645471083835 48-49 22.497714425207857 27.77200733298551 27.90938114771754 21.820897094089098 50-51 22.762002753285604 27.84629235320177 27.685525244008748 21.706179649503884 52-53 21.757953981478547 27.979007613435975 28.240462085933622 22.022576319151856 54-55 21.638131809943648 27.835153623288235 28.542892689709802 21.98382187705832 56-57 21.715748272460157 28.104810147169918 28.19062538119298 21.988816199176945 58-59 21.89516924016873 27.954147104512277 28.1788184928168 21.971865162502194 60-61 21.6973744223471 28.0522017503851 28.3157491631025 21.934674664165296 62-63 21.795701722880917 28.005686302763362 28.298388952571845 21.900223021783873 64-65 21.44123579304126 27.90183530600776 28.28606029980005 22.37086860115093 66-67 21.730639946217625 28.052416471918985 27.985401927201714 22.231541654661672 68-69 21.765117726376932 27.89864467724648 28.122298862496976 22.213938733879612 70-71 21.532908009787686 27.807953876276343 28.12450174005659 22.53463637387938 72-73 21.70502721147292 27.91734633800469 28.061897396181738 22.315729054340647 74-75 21.78944522593536 28.019617534121583 27.778200205299775 22.412737034643282 76-77 21.77607907965889 27.83642890060502 27.92437035189264 22.463121667843446 78-79 21.66851816843639 27.892750255422282 27.976014732714656 22.462716843426673 80-81 21.83002900224629 28.016254846092764 27.759778173155723 22.393937978505214 82-83 21.897604567974923 27.859262629340392 27.93648077268231 22.30665203000238 84-85 21.74622526493482 27.882084466535375 27.838527827273957 22.53316244125585 86-87 21.77262279784309 27.896179483486037 27.841066847035297 22.49013087163557 88-89 21.788984011239247 27.845146284215396 27.70826347712041 22.65760622742495 90-91 21.688846183982086 27.929696359864902 27.6387577270909 22.74269972906211 92-93 21.751836214517965 27.80939238759582 27.7484138187143 22.690357579171916 94-95 21.645853887317337 27.772226294874585 27.661947788831448 22.91997202897663 96-97 21.66225936778734 27.68442338657126 27.698717328882594 22.954599916758806 98-99 21.592918868680595 27.6410005727509 27.619810887302343 23.146269671266158 100-101 21.981299303554476 27.643384607629816 27.55097325951627 22.82434282929944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3281.0 1 3507.5 2 2642.0 3 1621.0 4 2092.5 5 2680.0 6 2832.0 7 2695.0 8 2462.0 9 2195.5 10 1946.5 11 1706.0 12 1443.5 13 1201.0 14 1012.5 15 850.0 16 758.0 17 702.5 18 675.5 19 705.0 20 782.0 21 866.0 22 961.5 23 1133.5 24 1338.0 25 1577.5 26 1873.0 27 2286.0 28 2709.5 29 3182.5 30 3730.0 31 4413.0 32 5118.5 33 5781.5 34 6462.0 35 7014.5 36 7659.5 37 8089.0 38 8338.5 39 8721.0 40 8863.0 41 8867.0 42 8871.5 43 8905.0 44 8985.5 45 9014.5 46 8835.5 47 8559.0 48 8342.0 49 8085.0 50 7698.0 51 7229.5 52 6888.0 53 6362.0 54 5633.0 55 5053.5 56 4448.0 57 3846.0 58 3301.5 59 2707.0 60 2192.5 61 1794.5 62 1450.0 63 1089.5 64 804.0 65 602.0 66 440.0 67 326.0 68 233.5 69 168.5 70 137.0 71 112.5 72 84.0 73 60.5 74 35.0 75 21.0 76 20.0 77 11.5 78 7.0 79 6.5 80 4.0 81 2.5 82 1.0 83 0.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.711126894824394 2 21.8059475523822 3 17.628081718098727 4 22.86387170333265 5 22.728173305171868 6 16.497635558213865 7 15.313358628810677 8 12.361264275434104 9 3.1356423244425335 10-11 6.6759499822433215 12-13 3.6096521560344668 14-15 7.583596568288444 16-17 11.745574849068243 18-19 14.875235976897628 20-21 15.298405637277806 22-23 13.273770583727407 24-25 15.77484532999383 26-27 13.260499803741988 28-29 13.317881908749369 30-31 14.207397992560885 32-33 12.633969458514793 34-35 19.536083437692753 36-37 24.27319115530551 38-39 23.386478757406405 40-41 18.4910562419394 42-43 18.43573017326779 44-45 23.731145212239024 46-47 13.079381693800116 48-49 21.900899048615912 50-51 21.522588362834338 52-53 11.496046802863498 54-55 13.164613745537467 56-57 12.659015719332348 58-59 13.72815461393245 60-61 13.120876245303826 62-63 13.090035700267286 64-65 14.187398366385676 66-67 12.421263153959739 68-69 13.502925178968617 70-71 12.07883964785705 72-73 11.425767742659016 74-75 11.505579334965702 76-77 11.582587241359974 78-79 12.003700865404385 80-81 11.964636175024767 82-83 10.43868338909553 84-85 10.312891347825275 86-87 10.465411861460533 88-89 11.925758397039306 90-91 9.350853255079345 92-93 11.415300648586008 94-95 11.525578961140912 96-97 11.080914375432235 98-99 10.908768060410086 100-101 11.005027943402927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 267505.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.17837050571016 #Duplication Level Percentage of deduplicated Percentage of total 1 98.37610873501289 95.60029943561112 2 1.2195314889453075 2.3702416575221497 3 0.23166932103160773 0.6753974134204767 4 0.07734907604035533 0.30066628678895996 5 0.0322666277582116 0.15678091536286592 6 0.017179122146771315 0.10016634581650764 7 0.010799312069655656 0.07346216846582496 8 0.00946975610125729 0.07362043736053522 9 0.00626087377498886 0.054757936025581655 >10 0.01782121449938944 0.2576568522534683 >50 0.0 0.0 >100 0.0011583544646732156 0.14783799247304394 >500 3.861181548910719E-4 0.18911255889952827 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 504 0.18840769331414367 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.869123941608568E-4 0.0 22-23 0.0 0.0 0.0 3.738247883217136E-4 0.0 24-25 0.0 0.0 0.0 3.738247883217136E-4 0.0 26-27 0.0 0.0 0.0 7.476495766434272E-4 0.0 28-29 0.0 0.0 0.0 0.0013083867591259977 0.0 30-31 0.0 0.0 0.0 0.00411207267153885 0.0 32-33 0.0 0.0 0.0 0.008971794919721127 0.0 34-35 0.0 0.0 0.0 0.013831517167903403 0.0 36-37 0.0 0.0 0.0 0.022429487299302817 0.0 38-39 0.0 0.0 0.0 0.03383114334311508 0.0 40-41 0.0 0.0 0.0 0.04915795966430534 0.0 42-43 0.0 0.0 0.0 0.06953141062783874 0.0 44-45 0.0 0.0 0.0 0.09588605820451954 0.0 46-47 0.0 0.0 0.0 0.1216799685987178 0.0 48-49 0.0 0.0 0.0 0.1467262294162726 0.0 50-51 0.0 0.0 0.0 0.18149193473019196 0.0 52-53 0.0 0.0 0.0 0.22616399693463674 0.0 54-55 0.0 0.0 0.0 0.2801816788471243 0.0 56-57 0.0 0.0 0.0 0.34092820694940285 0.0 58-59 0.0 0.0 0.0 0.4070951944823461 0.0 60-61 0.0 0.0 0.0 0.47232762004448514 0.0 62-63 0.0 0.0 0.0 0.5489617016504365 0.0 64-65 0.0 0.0 0.0 0.6283994691688006 0.0 66-67 0.0 0.0 0.0 0.7147529952711165 0.0 68-69 0.0 0.0 0.0 0.8194239360011962 0.0 70-71 0.0 0.0 0.0 0.9237210519429544 0.0 72-73 0.0 0.0 0.0 1.0407282106876505 3.738247883217136E-4 74-75 0.0 0.0 0.0 1.1541840339432907 3.738247883217136E-4 76-77 0.0 0.0 0.0 1.298854227023794 3.738247883217136E-4 78-79 0.0 0.0 0.0 1.4633371338853478 3.738247883217136E-4 80-81 0.0 0.0 0.0 1.625016354834489 3.738247883217136E-4 82-83 0.0 0.0 0.0 1.7945458963383862 3.738247883217136E-4 84-85 0.0 0.0 0.0 1.963140875871479 3.738247883217136E-4 86-87 0.0 0.0 0.0 2.147436496514084 3.738247883217136E-4 88-89 0.0 0.0 0.0 2.3420122988355354 3.738247883217136E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 200 0.0 50.415276 1 GTACTAG 40 4.0084773E-4 50.415276 1 GTATCAA 335 0.0 45.14801 1 GTGATAC 50 0.0025991176 34.480915 9 TATCAAC 500 0.0 34.28239 1 TCAACGC 500 0.0 34.247795 3 CAACGCA 500 0.0 34.230526 4 GTCTAGG 60 0.002956263 33.584743 2 GTAATAC 60 0.0029599227 33.57627 3 ATCAACG 515 0.0 33.258675 2 AACGCAG 540 0.0 31.346706 5 TAAGGTG 95 3.0747044E-5 30.545364 5 TACCCTA 80 4.0322435E-4 30.227184 5 CTTAGAC 70 0.006293027 28.77966 3 CTATAGC 90 6.3363876E-4 27.980223 3 ACGCAGA 635 0.0 27.078941 6 GTACATG 525 0.0 25.927858 1 CGCAGAG 680 0.0 25.298424 7 GTGTAGC 100 0.001165396 25.207638 1 TACATGG 520 0.0 25.188557 2 >>END_MODULE