FastQCFastQC Report
Sat 18 Jun 2016
SRR1930940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267505
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.2276592960879236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.1816788471243528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.14616549223379002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3550.049.4761541
GTCGTCC608.322489E-539.5438468
GTATCAA5650.031.926971
ACGTAGA600.003957652531.6469062
CCCGCGA350.00842856427.1157884-85
ATCAACG7150.026.5568472
CAACGCA7700.026.5094264
TCAACGC7350.026.4800663
GTACATG7050.026.26021
ATATAGT1457.892504E-726.1905444
TATCAAC7300.026.011161
AACGCAG7950.025.6661955
CCTATAT1301.0032061E-525.5609651
ATTATAG1501.0607819E-625.3175263
GGGTAGA950.001228555624.9844021
TAGTACC1151.2835895E-424.7671454
TACATGG7350.024.5425012
AGTACAT5650.024.3653182
GCCTTAG1000.001656347123.735181
TAGACTC1000.001659957123.7263075
Produced by FastQC (version 0.11.5)