##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930940_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267505 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.266193155268127 31.0 31.0 33.0 30.0 34.0 2 31.3790620736061 31.0 31.0 34.0 30.0 34.0 3 31.45882880693819 31.0 31.0 34.0 28.0 34.0 4 35.32793779555522 37.0 35.0 37.0 33.0 37.0 5 35.0953103680305 37.0 35.0 37.0 32.0 37.0 6 35.14988504887759 37.0 35.0 37.0 32.0 37.0 7 35.02703874693931 37.0 35.0 37.0 32.0 37.0 8 34.9984187211454 37.0 35.0 37.0 32.0 37.0 9 36.524969626735945 38.0 35.0 39.0 32.0 39.0 10-11 36.452656959683 38.0 35.0 39.0 32.0 39.0 12-13 36.35250556064372 38.0 35.0 39.0 32.0 39.0 14-15 37.363125922879945 39.0 36.0 41.0 32.0 41.0 16-17 37.3356815760453 39.0 36.0 41.0 32.0 41.0 18-19 37.33191342217903 39.0 36.0 41.0 32.0 41.0 20-21 37.307326965851104 39.0 36.0 40.0 31.5 41.0 22-23 37.17554251322405 39.0 36.0 40.0 31.0 41.0 24-25 37.06061382030242 39.0 36.0 40.0 31.0 41.0 26-27 36.784501224276184 39.0 35.5 40.0 30.5 41.0 28-29 36.61912861441842 38.5 35.0 40.0 30.0 41.0 30-31 36.38619651969122 38.0 35.0 40.0 30.0 41.0 32-33 36.15996897254257 38.0 35.0 40.0 30.0 41.0 34-35 36.34103474701408 38.0 35.0 40.0 30.0 41.0 36-37 36.37357993308537 38.0 35.0 40.0 30.0 41.0 38-39 36.351365395039345 38.0 35.0 40.0 30.0 41.0 40-41 36.25456907347527 38.0 35.0 40.0 30.0 41.0 42-43 36.13529653651334 38.0 35.0 40.0 30.0 41.0 44-45 35.94724958411992 38.0 35.0 40.0 29.0 41.0 46-47 35.73828152744808 38.0 34.5 40.0 28.0 41.0 48-49 35.532657333507785 38.0 34.0 40.0 27.5 41.0 50-51 35.309678323769646 38.0 34.0 40.0 27.0 41.0 52-53 35.12850974748136 38.0 34.0 40.0 27.0 41.0 54-55 34.971021102409296 38.0 33.5 40.0 26.0 41.0 56-57 34.727066783798435 38.0 33.0 40.0 26.0 41.0 58-59 34.480925590175886 37.5 33.0 40.0 26.0 41.0 60-61 34.18703014896918 37.0 33.0 40.0 25.5 41.0 62-63 33.90576250911198 37.0 32.0 39.5 25.0 41.0 64-65 33.61530438683389 36.0 32.0 39.0 24.0 41.0 66-67 33.284433935814285 36.0 32.0 39.0 23.0 40.0 68-69 32.91523709837199 35.5 31.0 39.0 22.5 40.0 70-71 32.501844825330366 35.0 31.0 38.0 22.0 40.0 72-73 32.09187865647371 35.0 31.0 37.5 21.5 40.0 74-75 31.563249285060092 34.5 30.0 37.0 20.0 39.0 76-77 30.17773499560756 33.5 28.5 35.5 19.5 38.5 78-79 30.934825517280053 34.0 30.0 36.0 20.0 39.0 80-81 30.93810396067363 34.0 30.0 36.0 20.0 37.5 82-83 30.636922674342536 34.0 30.0 35.0 19.5 37.0 84-85 30.30629707855928 34.0 30.0 35.0 18.0 37.0 86-87 29.962490420739798 34.0 29.5 35.0 17.5 36.0 88-89 29.6131081662025 34.0 29.0 35.0 13.0 36.0 90-91 29.273387413319377 34.0 29.0 35.0 7.5 35.5 92-93 28.919724864955796 34.0 29.0 35.0 4.5 35.0 94-95 28.505956898001905 33.5 29.0 35.0 2.0 35.0 96-97 28.05125324760285 33.0 28.5 35.0 2.0 35.0 98-99 27.571862208183024 33.0 27.0 35.0 2.0 35.0 100-101 26.360346535578774 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44125586332066646 1101 2 0.4309684510320686 1101 3 0.5930725418435294 1101 4 0.3056109269510898 1101 5 0.6135503240206717 1101 6 0.5588134251938897 1101 7 0.6319643492652389 1101 8 0.630522088157079 1101 9 0.3465383705636995 1101 10-11 0.401427182719047 1101 12-13 0.7689650438997333 1101 14-15 0.689993066540346 1101 16-17 0.6449076998077956 1101 18-19 0.6918730041849841 1101 20-21 0.523517689597405 1101 22-23 0.431243609307117 1101 24-25 0.3151371647177257 1101 26-27 0.5349468559989887 1101 28-29 0.7146700791998128 1101 30-31 0.8139928198129098 1101 32-33 1.0107859219343922 1101 34-35 0.5097581145130547 1101 36-37 0.2805927364721086 1101 38-39 0.5543829377718055 1101 40-41 0.6925078395465292 1101 42-43 0.563715081066924 1101 44-45 0.5989538019059921 1101 46-47 0.796331277721329 1101 48-49 0.38799293770251353 1101 50-51 0.5887560541515526 1101 52-53 0.5019674980678914 1101 54-55 0.4019149599081402 1101 56-57 0.2988941121986173 1101 58-59 0.09745209276805866 1101 60-61 0.12551640653989438 1101 62-63 0.3233603383361938 1101 64-65 0.29749597163349506 1101 66-67 0.3358484254520704 1101 68-69 0.12163780388099354 1101 70-71 0.3015531443004207 1101 72-73 0.15618686903589918 1101 74-75 -0.013809604591887847 1101 76-77 0.06769057197829298 1101 78-79 -0.09729267901045802 1101 80-81 -0.3279184623783351 1101 82-83 0.09519501325426205 1101 84-85 0.07156440937143316 1101 86-87 0.1324308661343494 1101 88-89 -0.07055499964129908 1101 90-91 -0.25992739406283505 1101 92-93 0.037463969493124694 1101 94-95 0.13090102263599945 1101 96-97 0.12228173359438088 1101 98-99 -0.08053089364241472 1101 100-101 0.2731965535892016 1102 1 0.23718205127019942 1102 2 0.38330308138226243 1102 3 0.3457255308778393 1102 4 0.005115702331352168 1102 5 0.12180406768297303 1102 6 0.370922846252725 1102 7 0.4260629225466914 1102 8 0.07654850013680203 1102 9 0.44546538388760126 1102 10-11 0.5824642066549046 1102 12-13 0.32812669936488703 1102 14-15 0.5449703687452754 1102 16-17 0.6005364582089214 1102 18-19 0.48507250896036425 1102 20-21 0.4172433705312599 1102 22-23 0.4918349310852079 1102 24-25 0.5885645317734998 1102 26-27 0.6095621725890155 1102 28-29 0.5093950526699231 1102 30-31 1.0562328268875447 1102 32-33 0.9758917467192063 1102 34-35 0.5497150771353887 1102 36-37 0.5869171675533522 1102 38-39 0.4293976765997769 1102 40-41 0.4248065071564824 1102 42-43 0.4135337934829124 1102 44-45 0.9718387800925257 1102 46-47 0.7520853161601906 1102 48-49 0.9508024530898425 1102 50-51 0.7826600749038235 1102 52-53 0.7428662717974035 1102 54-55 0.26294992040807585 1102 56-57 0.8324544234826661 1102 58-59 0.8955964743268297 1102 60-61 0.7483940153124351 1102 62-63 0.34055318116133293 1102 64-65 0.31531521464728485 1102 66-67 0.519892170293474 1102 68-69 0.6521324389476106 1102 70-71 0.7931446429444478 1102 72-73 0.9083829544031943 1102 74-75 1.020060222079497 1102 76-77 1.260349161767234 1102 78-79 1.1815438379097145 1102 80-81 0.926031543517194 1102 82-83 0.7681920065333578 1102 84-85 1.18940133897879 1102 86-87 1.1919757311266856 1102 88-89 1.0262127753852326 1102 90-91 1.0009572303918297 1102 92-93 1.0878781305590373 1102 94-95 0.8520350280598876 1102 96-97 0.8698277187376426 1102 98-99 0.8704182868787491 1102 100-101 0.8219820741646089 1103 1 0.31727689281542126 1103 2 0.5417000279471473 1103 3 0.47393510756069546 1103 4 0.36250640323350325 1103 5 0.6423916226216733 1103 6 0.34779076205193604 1103 7 0.3109229734372789 1103 8 0.5387853726790155 1103 9 0.2885141925852679 1103 10-11 0.5118246045267512 1103 12-13 0.46639873267508847 1103 14-15 0.5021904705264504 1103 16-17 0.8498858683407704 1103 18-19 0.7183306630200477 1103 20-21 0.8308884086992023 1103 22-23 0.5119309292346443 1103 24-25 0.42905435365644706 1103 26-27 0.24050422671809457 1103 28-29 0.542661881261175 1103 30-31 0.8111339372275879 1103 32-33 0.6353440946257507 1103 34-35 0.5870444780144055 1103 36-37 0.9626255876320045 1103 38-39 1.0996498163175659 1103 40-41 1.2907271641076719 1103 42-43 1.0134210245724304 1103 44-45 1.200484974864672 1103 46-47 0.48149776122149035 1103 48-49 0.5866889902168424 1103 50-51 0.4976727975501305 1103 52-53 0.580204347208003 1103 54-55 0.6298681484904307 1103 56-57 0.3677597669941193 1103 58-59 0.42735971744503587 1103 60-61 0.6089942928974494 1103 62-63 0.5489298871525463 1103 64-65 0.3572132021559611 1103 66-67 0.14633792555602554 1103 68-69 0.7769011181056058 1103 70-71 0.7096233614269778 1103 72-73 0.44866169601780825 1103 74-75 0.7900116447026697 1103 76-77 0.7775102767336897 1103 78-79 0.35857656980886077 1103 80-81 0.4486431558234649 1103 82-83 0.264418585298106 1103 84-85 0.42682326819461025 1103 86-87 0.23962782458030674 1103 88-89 0.39249487115205994 1103 90-91 0.4501967631237278 1103 92-93 0.025493790980039677 1103 94-95 -0.134967632129797 1103 96-97 0.24946859313042324 1103 98-99 0.42206066485700333 1103 100-101 0.347022555311959 1104 1 0.5201201276865355 1104 2 0.3506943857300868 1104 3 0.33287968107546817 1104 4 0.09273098559880566 1104 5 0.1422256750611055 1104 6 0.4229650069905375 1104 7 0.3680919080539411 1104 8 0.30612689275866956 1104 9 0.3973757923197496 1104 10-11 0.49567237529785757 1104 12-13 0.4608671473227304 1104 14-15 0.6600066006293375 1104 16-17 0.38503909325503827 1104 18-19 0.6764427329392859 1104 20-21 0.5195984429950258 1104 22-23 0.6509258401761215 1104 24-25 0.7525138071358128 1104 26-27 0.6173849657378909 1104 28-29 0.4104984782298686 1104 30-31 0.3362888216174511 1104 32-33 0.6872718521210501 1104 34-35 0.66318675038967 1104 36-37 0.566577905366259 1104 38-39 0.6644108518303469 1104 40-41 0.686664214666358 1104 42-43 0.8391760859730297 1104 44-45 0.9104916127670961 1104 46-47 0.6773158826951047 1104 48-49 0.8761153648157958 1104 50-51 1.0417180459183086 1104 52-53 1.3305639002559033 1104 54-55 0.9376040706057083 1104 56-57 1.2201355829029552 1104 58-59 1.2563507562767668 1104 60-61 1.1872938835601374 1104 62-63 0.29501629051574696 1104 64-65 0.5495049379674484 1104 66-67 0.6873822888705519 1104 68-69 0.4849717011347039 1104 70-71 0.629360057964206 1104 72-73 0.2894764985402105 1104 74-75 0.5133079164141456 1104 76-77 0.985069188117695 1104 78-79 0.5590965388319837 1104 80-81 0.9240552589322135 1104 82-83 0.6719304782066082 1104 84-85 0.8697373073582355 1104 86-87 0.8988268510212833 1104 88-89 0.789635041524889 1104 90-91 0.42231757294782213 1104 92-93 0.6370382096104201 1104 94-95 0.41931104914025497 1104 96-97 0.5010036080657052 1104 98-99 0.9766435833214366 1104 100-101 1.0453022322673746 1105 1 0.16446583804303927 1105 2 0.4836680448859134 1105 3 0.4371322992919069 1105 4 0.22707986955099102 1105 5 -0.11896124972467703 1105 6 0.25524047509590275 1105 7 0.3123159639092208 1105 8 0.18775177374883611 1105 9 0.4075312985082604 1105 10-11 0.3271279978515409 1105 12-13 0.47726405835626196 1105 14-15 0.34986696241924875 1105 16-17 0.38649365794349677 1105 18-19 0.5085462979451023 1105 20-21 0.3155097938889284 1105 22-23 0.3391861808087171 1105 24-25 0.3986314417491599 1105 26-27 0.4470289940886829 1105 28-29 0.5164040108650099 1105 30-31 0.6952092701523114 1105 32-33 0.21719621475724438 1105 34-35 0.7033591824218277 1105 36-37 0.7979462540386422 1105 38-39 0.34717029327750026 1105 40-41 0.45635373122195233 1105 42-43 0.41589776163154824 1105 44-45 0.5392602576417431 1105 46-47 0.25927399123207095 1105 48-49 0.43981207485494167 1105 50-51 0.6725627513028414 1105 52-53 1.133695181330701 1105 54-55 0.35177153067130007 1105 56-57 0.2567244964753641 1105 58-59 0.022932793725196632 1105 60-61 0.21231239620162512 1105 62-63 0.35524849193328834 1105 64-65 0.6898175371423179 1105 66-67 0.29491428929370045 1105 68-69 0.3494062721703486 1105 70-71 0.24538142684180997 1105 72-73 0.7614298466836544 1105 74-75 0.7750369971292592 1105 76-77 0.7695423712341629 1105 78-79 0.7001099737203802 1105 80-81 0.7467570467244506 1105 82-83 0.8829481445559146 1105 84-85 0.8918731372535085 1105 86-87 0.5809383500163143 1105 88-89 0.2896085948941902 1105 90-91 0.2874397563816551 1105 92-93 0.27847479059000335 1105 94-95 0.5125221990397577 1105 96-97 0.42701915868455487 1105 98-99 -0.11918578301728999 1105 100-101 0.6894892628396789 1106 1 -0.06510134608361895 1106 2 -0.07796452425154143 1106 3 -0.10465859204149552 1106 4 -0.13700977782229984 1106 5 0.11145408902313392 1106 6 0.05967658078110816 1106 7 0.11503005869693084 1106 8 -0.018735324020262567 1106 9 -0.31082996395704043 1106 10-11 0.01406898685122826 1106 12-13 -0.2010026220179526 1106 14-15 -0.315651696982421 1106 16-17 -0.2596588042750696 1106 18-19 -0.15977384187189614 1106 20-21 -0.20335522101803605 1106 22-23 -0.19883941803984584 1106 24-25 -0.11947537432979516 1106 26-27 0.34875871548273807 1106 28-29 0.022919380454816007 1106 30-31 0.0867225836096992 1106 32-33 -0.29792920932006695 1106 34-35 -0.22599981829782223 1106 36-37 0.050452165419336836 1106 38-39 -0.11835093628967996 1106 40-41 -0.7519967365481719 1106 42-43 -0.2685244651598495 1106 44-45 -0.2836296364674382 1106 46-47 -0.13369146574334678 1106 48-49 0.04755341339714647 1106 50-51 0.005077915279407819 1106 52-53 0.07094459019263155 1106 54-55 0.13328175993005686 1106 56-57 0.32013047982069764 1106 58-59 0.4229919075321291 1106 60-61 0.048490046042275026 1106 62-63 -0.2402460079125035 1106 64-65 0.1787915270672613 1106 66-67 0.29176583653333665 1106 68-69 -0.034113826524006186 1106 70-71 -0.11239846418316546 1106 72-73 0.10721991556409449 1106 74-75 0.1229731542177781 1106 76-77 0.05273605890765154 1106 78-79 0.22969257884015093 1106 80-81 -0.06601924606880516 1106 82-83 0.4772989207459055 1106 84-85 0.3318469215652158 1106 86-87 0.4337064182799075 1106 88-89 0.4147371031701219 1106 90-91 0.13121224532994447 1106 92-93 0.05424069991456193 1106 94-95 0.45220041431682034 1106 96-97 0.040042187367600235 1106 98-99 -0.3876909747516386 1106 100-101 -0.26465898430743806 1107 1 0.11932825558135107 1107 2 0.2862240435815764 1107 3 0.05542562459855205 1107 4 0.09930501817014914 1107 5 0.03823643256895082 1107 6 0.031810068995618224 1107 7 -0.06964154480726137 1107 8 -0.04521032523027202 1107 9 -0.39427219811801706 1107 10-11 0.16528988613006845 1107 12-13 -0.42296670894501176 1107 14-15 -0.26495231166708777 1107 16-17 -0.06337716253459291 1107 18-19 -0.33549137732516954 1107 20-21 -0.4266140177848925 1107 22-23 -0.2043147096520599 1107 24-25 -0.16508667097564 1107 26-27 -0.4244311107599401 1107 28-29 -0.10543234501830057 1107 30-31 -0.30832121872320073 1107 32-33 -0.5695823238837292 1107 34-35 -0.390264616710283 1107 36-37 -0.06645287305895664 1107 38-39 -0.04373571921403396 1107 40-41 -0.34842841942896285 1107 42-43 0.14617593656320338 1107 44-45 -0.2729089549902639 1107 46-47 -0.18410649889760577 1107 48-49 0.2897004537146515 1107 50-51 0.23798653538491266 1107 52-53 -0.5197274489148711 1107 54-55 0.10301942993635294 1107 56-57 -0.13962461431802353 1107 58-59 0.07911334405503823 1107 60-61 0.06559957607469613 1107 62-63 0.03633184407917156 1107 64-65 -0.4104345510509262 1107 66-67 -0.2687285732241804 1107 68-69 -0.31026212525107866 1107 70-71 -0.09804170507180032 1107 72-73 -0.2384071112013082 1107 74-75 -0.3066345608008554 1107 76-77 -0.3700593043371079 1107 78-79 0.2582971962352403 1107 80-81 0.1941508812241608 1107 82-83 -0.1481427326106619 1107 84-85 -0.022837024032767772 1107 86-87 -0.021238889304431297 1107 88-89 0.30700393439803975 1107 90-91 0.1574512947467177 1107 92-93 0.15112008619789918 1107 94-95 -0.026733325579598954 1107 96-97 -0.546116517438552 1107 98-99 -0.143015015214349 1107 100-101 0.41624949059697514 1108 1 0.24475780884595721 1108 2 0.43040453065762563 1108 3 0.4198362028145972 1108 4 0.4622962029901103 1108 5 0.4683126315828403 1108 6 0.38310993452677167 1108 7 0.4369324877640821 1108 8 0.49453268986012233 1108 9 0.6872308647835155 1108 10-11 0.4739332448630691 1108 12-13 0.39564975601838626 1108 14-15 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-1.0472228548287816 2316 100-101 -0.8193168067608276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 10.0 6 18.0 7 58.0 8 102.0 9 213.0 10 327.0 11 507.0 12 653.0 13 809.0 14 975.0 15 1230.0 16 1515.0 17 1756.0 18 1557.0 19 1450.0 20 1495.0 21 1472.0 22 1726.0 23 2107.0 24 2443.0 25 3020.0 26 3982.0 27 5093.0 28 6506.0 29 7861.0 30 9688.0 31 11751.0 32 14090.0 33 16983.0 34 20845.0 35 26024.0 36 33186.0 37 43479.0 38 38285.0 39 6284.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.71189184541012 22.90807532835581 15.09595504525758 22.284077780976485 2 15.881673439328292 22.719880970627706 39.534648986717606 21.863796603326392 3 18.172744061549047 26.96135240416607 31.483696830583114 23.382206703701765 4 12.901995392945823 18.37474197505011 39.44790139707422 29.275361234929846 5 12.645607476635515 38.68373831775701 36.08373831775701 12.586915887850466 6 29.857759668043588 39.6022504252257 17.797798171996785 12.742191734733929 7 25.793162744621593 33.43077699482252 23.557316685669427 17.21874357488645 8 24.65972598643016 37.14696921552868 21.041475860264296 17.151828937776862 9 25.53821423898619 16.122315470738865 20.991383338629184 37.34808695164576 10-11 23.84815237098372 27.127156501747628 29.70280929328424 19.32188183398441 12-13 24.499728977028468 24.636735761948376 29.821872488364704 21.041662772658455 14-15 21.651184090017008 26.478009756826975 27.058372740696434 24.81243341245958 16-17 21.310442795461768 29.52879385432048 27.782097530887274 21.378665819330482 18-19 21.28240593633764 28.285826433150785 29.76262125941571 20.669146371095866 20-21 21.914169828601334 27.647520607091458 29.748789742247812 20.689519822059403 22-23 21.615670735126447 27.480420926711652 29.58692360890451 21.316984729257396 24-25 21.681398561140323 27.621133929088774 29.624098376282927 21.073369133487972 26-27 21.520974805560382 27.913697034947727 29.43519520597235 21.13013295351954 28-29 21.57548457038186 27.97592568363208 29.385245135604944 21.063344610381115 30-31 21.772004725445626 27.805920265582007 29.271593492044502 21.15048151692786 32-33 21.822308386911025 27.74816645572105 29.137790174857404 21.291734982510523 34-35 21.817090792850827 27.749498544704764 29.443697739759187 20.98971292268522 36-37 21.940314688059573 27.67482981499273 29.19313054433034 21.191724952617356 38-39 21.77956882726549 27.843426055034897 29.258176543814464 21.118828573885153 40-41 21.900040934655955 27.768276202385422 29.239120073121633 21.09256278983699 42-43 21.83842084602875 27.7937080583958 29.141821036693642 21.226050058881807 44-45 21.807683392775697 27.886693135341662 29.024322768294414 21.281300703588222 46-47 21.95415104285442 27.81069734230075 28.80794206821266 21.427209546632174 48-49 21.948661774107318 27.922170172834704 28.62464997513842 21.504518077919556 50-51 21.858813747851197 28.012913422394004 28.715511462160453 21.412761367594353 52-53 22.07124824684432 27.98915381019168 28.535951379149132 21.403646563814867 54-55 21.902778530741806 27.98907506313993 28.698149079400213 21.40999732671805 56-57 21.81876300338158 28.097959275231467 28.555005355570636 21.528272365816317 58-59 21.813096831604685 28.063517464685518 28.73484786168837 21.38853784202143 60-61 21.88410513047804 28.124497406148198 28.689272636827084 21.30212482654668 62-63 21.82482516566699 28.057121903215304 28.59818224678488 21.519870684332822 64-65 21.631461767292183 28.010626952796123 28.603154408875753 21.75475687103594 66-67 21.762656558134662 27.99261318774253 28.57356688732305 21.67116336679976 68-69 21.66923618075409 28.027099642263764 28.64116361568052 21.66250056130162 70-71 21.703665732491704 27.923474981198016 28.515784314092326 21.85707497221796 72-73 21.709236731456958 28.005902949230148 28.507541410423304 21.777318908889587 74-75 21.781254391306383 28.017424703733194 28.4373038549815 21.76401704997892 76-77 21.640442529963746 27.92580012671563 28.368929927757097 22.064827415563528 78-79 21.575026870414504 27.947661105659144 28.53161362680499 21.94569839712136 80-81 21.668582536611808 28.061793815011075 28.293006607414885 21.976617040962235 82-83 21.74781779779817 28.028634978785444 28.37909571783705 21.844451505579336 84-85 21.720528588250687 27.97555185884376 28.205641016055775 22.098278536849776 86-87 21.683146109418516 28.085643259004506 28.205454103661616 22.025756527915366 88-89 21.60326874227814 27.852532770599264 28.31290057887093 22.23129790825167 90-91 21.546947900466563 28.002003828209116 28.37884316305778 22.072205108266537 92-93 21.666124527497672 27.802018253264986 28.386406888532452 22.145450330704893 94-95 21.431735641615624 27.836583483021528 28.462937452453357 22.26874342290949 96-97 21.47156127922843 27.659483000317753 28.402085942318834 22.46686977813499 98-99 21.333619932337715 27.68602456028859 28.358161529691035 22.622193977682663 100-101 21.255303639184316 27.733126483617127 28.31591932861068 22.695650548587874 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 73.0 1 57.0 2 55.5 3 63.5 4 50.5 5 53.0 6 85.0 7 176.0 8 272.5 9 289.5 10 222.0 11 145.0 12 130.5 13 140.0 14 171.5 15 208.5 16 213.5 17 220.5 18 261.0 19 299.5 20 341.0 21 395.0 22 498.0 23 631.0 24 792.5 25 1042.0 26 1320.5 27 1671.5 28 2093.5 29 2617.0 30 3295.5 31 4118.5 32 5220.5 33 6141.5 34 6875.0 35 7600.0 36 8374.5 37 9370.5 38 9929.0 39 9976.0 40 9997.0 41 10188.0 42 10341.5 43 10438.0 44 10584.0 45 10755.5 46 10728.0 47 10482.0 48 10395.0 49 10091.0 50 9509.5 51 8946.0 52 8450.5 53 8100.5 54 7497.0 55 6574.5 56 5680.5 57 4831.5 58 4106.0 59 3440.0 60 2757.5 61 2161.0 62 1644.5 63 1235.0 64 901.0 65 642.5 66 445.5 67 311.0 68 230.0 69 161.0 70 112.5 71 81.5 72 64.5 73 51.0 74 37.5 75 24.5 76 17.0 77 13.5 78 9.5 79 5.5 80 3.5 81 3.0 82 2.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013457692379581691 2 0.002990598306573709 3 0.004112072671538849 4 0.03327040616063251 5 0.0018691239416085681 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.607371824825703E-4 26-27 0.0020560363357694247 28-29 0.0 30-31 0.006355021401469132 32-33 0.021868750116820247 34-35 0.012523130408777406 36-37 7.476495766434272E-4 38-39 7.476495766434272E-4 40-41 0.0020560363357694247 42-43 0.0074764957664342725 44-45 0.007102670978112559 46-47 0.0067288461897908455 48-49 0.008971794919721126 50-51 0.18541709500756995 52-53 0.04859722248182277 54-55 0.01626137829199454 56-57 0.00990635689052541 58-59 0.019065064204407393 60-61 0.053083119941683334 62-63 0.09420384665707184 64-65 0.09719444496364554 66-67 0.10168034242350611 68-69 0.10055886805854096 70-71 0.09196089792714156 72-73 0.06766228668623017 74-75 0.240182426496701 76-77 0.2863497878544326 78-79 0.006541933795629989 80-81 9.345619708042841E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 5.607371824825703E-4 90-91 0.006355021401469132 92-93 0.017195940262798824 94-95 0.0016822115474477114 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 267505.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.79264129876326 #Duplication Level Percentage of deduplicated Percentage of total 1 91.55271604355124 78.5454932745192 2 5.856254698101747 10.048471173368807 3 1.2820557678125546 3.2997285183885907 4 0.531443828778823 1.8237587909145168 5 0.2655550202646158 1.1391333299323996 6 0.15417327112795962 0.7936159288642809 7 0.08621317472046736 0.5177519181814462 8 0.055699479921115375 0.3822884401119934 9 0.052723751567226675 0.40709789155189674 >10 0.15358311243988504 2.030041681956342 >50 0.008275235571496922 0.45736004424574106 >100 8.710774285781678E-4 0.3276999663426038 >500 4.355387142890839E-4 0.227559041622173 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 609 0.2276592960879236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 486 0.1816788471243528 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 391 0.14616549223379002 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.869123941608568E-4 0.0 22-23 0.0 0.0 0.0 3.738247883217136E-4 0.0 24-25 0.0 0.0 0.0 3.738247883217136E-4 0.0 26-27 0.0 0.0 0.0 7.476495766434272E-4 0.0 28-29 0.0 0.0 0.0 0.0013083867591259977 0.0 30-31 0.0 0.0 0.0 0.005607371824825704 0.0 32-33 0.0 0.0 0.0 0.01570064110951197 0.0 34-35 0.0 0.0 0.0 0.0315881946131848 0.0 36-37 0.0 0.0 0.0 0.057008280219061326 0.0 38-39 0.0 0.0 0.0 0.08934412440888956 0.0 40-41 0.0 0.0 0.0 0.12448365451113064 0.0 42-43 0.0 0.0 0.0 0.16541746883235828 0.0 44-45 0.0 0.0 0.0 0.21625764004411133 0.0 46-47 0.0 0.0 0.0 0.26990149716827727 0.0 48-49 3.738247883217136E-4 0.0 0.0 0.3256013906282126 0.0 50-51 3.738247883217136E-4 0.0 0.0 0.3910207285845124 0.0 52-53 3.738247883217136E-4 0.0 0.0 0.4564400665408123 0.0 54-55 3.738247883217136E-4 0.0 0.0 0.5304573746285116 0.0 56-57 3.738247883217136E-4 0.0 0.0 0.6151286891833797 0.0 58-59 3.738247883217136E-4 0.0 0.0 0.7171828563952076 0.0 60-61 3.738247883217136E-4 0.0 0.0 0.8153118633296574 0.0 62-63 3.738247883217136E-4 0.0 0.0 0.9244687015195978 0.0 64-65 3.738247883217136E-4 0.0 0.0 1.0424104222350983 0.0 66-67 3.738247883217136E-4 0.0 0.0 1.1753051344834677 1.869123941608568E-4 68-69 3.738247883217136E-4 0.0 0.0 1.3319377207902656 3.738247883217136E-4 70-71 3.738247883217136E-4 0.0 0.0 1.4850189716080073 3.738247883217136E-4 72-73 3.738247883217136E-4 0.0 0.0 1.6622119212724997 3.738247883217136E-4 74-75 3.738247883217136E-4 0.0 0.0 1.839217958542831 3.738247883217136E-4 76-77 3.738247883217136E-4 0.0 0.0 2.0560363357694245 3.738247883217136E-4 78-79 3.738247883217136E-4 0.0 0.0 2.2930412515653913 3.738247883217136E-4 80-81 3.738247883217136E-4 0.0 0.0 2.5500457935365692 3.738247883217136E-4 82-83 3.738247883217136E-4 0.0 0.0 2.8257415749238333 3.738247883217136E-4 84-85 3.738247883217136E-4 0.0 0.0 3.1092876768658533 3.738247883217136E-4 86-87 3.738247883217136E-4 0.0 0.0 3.4137679669538885 3.738247883217136E-4 88-89 3.738247883217136E-4 0.0 0.0 3.7487149772901445 3.738247883217136E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 355 0.0 49.476154 1 GTCGTCC 60 8.322489E-5 39.543846 8 GTATCAA 565 0.0 31.92697 1 ACGTAGA 60 0.0039576525 31.646906 2 CCCGCGA 35 0.008428564 27.11578 84-85 ATCAACG 715 0.0 26.556847 2 CAACGCA 770 0.0 26.509426 4 TCAACGC 735 0.0 26.480066 3 GTACATG 705 0.0 26.2602 1 ATATAGT 145 7.892504E-7 26.190544 4 TATCAAC 730 0.0 26.01116 1 AACGCAG 795 0.0 25.666195 5 CCTATAT 130 1.0032061E-5 25.560965 1 ATTATAG 150 1.0607819E-6 25.317526 3 GGGTAGA 95 0.0012285556 24.984402 1 TAGTACC 115 1.2835895E-4 24.767145 4 TACATGG 735 0.0 24.542501 2 AGTACAT 565 0.0 24.365318 2 GCCTTAG 100 0.0016563471 23.73518 1 TAGACTC 100 0.0016599571 23.726307 5 >>END_MODULE