FastQCFastQC Report
Sat 18 Jun 2016
SRR1930939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882188
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33830.17973762450934763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.10370908750879294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16250.056.0183871
GTATCAA33350.049.3125341
ATCAACG42550.038.4017523
TCAACGC44550.037.5713124
TATCAAC44100.036.9445572
CAACGCA47350.034.8837745
TAACGCA2400.033.5095444
AACGCAG50500.033.182116
GTACATG40900.030.2494321
TACATGG41950.029.1285132
ACGCAGA58050.028.8076787
TATAACG2450.028.734652
CGCAGAG60200.028.5227728
ACATGGG42500.027.4451923
CATGGGG31400.026.412784
TATCACG2251.4551915E-924.5840912
GCAGAGT65100.024.5037129
ATAACGC2853.8198777E-1122.9332543
AGAGTAC59000.021.8788910-11
ATGGGGG20700.020.5319215
Produced by FastQC (version 0.11.5)