FastQCFastQC Report
Sat 18 Jun 2016
SRR1930939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882188
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43700.23217659447409078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36700.19498583563384742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.1359056587333465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.055.6095961
ATAACGC3700.043.629433
GTATCAA47400.042.6790161
TAACGCA3650.039.0207944
TATAACG4150.036.611352
ATCAACG60550.033.482363
TCAACGC60900.033.287284
CAACGCA62750.032.60165
TATCAAC62450.032.3124852
AACGCAG67300.030.8190126
GTACATG51700.027.4641
ACGCAGA75900.026.9518017
TACATGG54000.026.2900432
CGCAGAG77950.026.2430028
TATCACG2451.0913936E-1125.1936552
ACATGGG53350.025.0967243
GTATAAC7850.023.5927241
GCAGAGT87200.023.0781889
CATGGGG36500.023.022274
CCGTCGA1050.00220679322.601289
Produced by FastQC (version 0.11.5)