FastQCFastQC Report
Sat 18 Jun 2016
SRR1930938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332266
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42470.18209758235124124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.052.7449341
GTATCAA33750.039.4922831
TCAACGC39000.032.8653954
ATCAACG39350.032.2030873
CAACGCA40900.031.5836265
TATCAAC41400.030.974912
AACGCAG45250.029.1509176
GTACATG47550.028.4523141
TACATGG50950.026.643762
ACGCAGA51850.025.1052427
ACATGGG53700.024.530323
CGCAGAG54050.024.1721128
GTGGTAT10250.021.5096421
CATGGGG41250.021.2411294
GCAGAGT60600.020.6283119
GAGTACA37100.020.2591741
CTAACGC2551.5321893E-719.6418113
CAGAGTA59500.018.8048910-11
AGTACAT37600.018.6513652
GAGTACT37250.018.10323112-13
Produced by FastQC (version 0.11.5)