FastQCFastQC Report
Sat 18 Jun 2016
SRR1930938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2332266
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30410.13038821472336345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27310.11709642039115606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27150.049.8408051
GTATCAA44500.037.6638451
ATCAACG52800.030.4803473
TCAACGC52800.030.389134
CAACGCA54000.029.7902245
TATCAAC56200.028.9742322
AACGCAG58150.027.7451926
GTACATG61400.024.4359191
GTGGTAT13400.023.7399621
ACGCAGA68400.023.6568457
TACATGG62800.023.3589292
CGCAGAG70050.023.0996178
ACATGGG64350.021.9847663
TATACCG1856.612703E-620.5204145
GCAGAGT81250.019.3313929
CATGGGG50200.019.2913194
TGGTATC15100.019.1781622
CAGAGTA77950.017.98870510-11
GAGTACT51300.017.57497812-13
AGAGTAC74000.017.57022910-11
Produced by FastQC (version 0.11.5)