##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930938_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2332266 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.180880740018505 31.0 31.0 33.0 30.0 34.0 2 31.3042148708595 31.0 31.0 34.0 28.0 34.0 3 31.384878482986075 31.0 31.0 34.0 28.0 34.0 4 35.2547346657714 37.0 35.0 37.0 33.0 37.0 5 35.041064784205574 35.0 35.0 37.0 32.0 37.0 6 35.07959383706661 36.0 35.0 37.0 32.0 37.0 7 34.9553382847411 36.0 35.0 37.0 32.0 37.0 8 34.9337052463141 36.0 35.0 37.0 32.0 37.0 9 36.429900362994616 38.0 35.0 39.0 32.0 39.0 10-11 36.355800324662795 38.0 35.0 39.0 32.0 39.0 12-13 36.24116653074735 38.0 35.0 39.0 32.0 39.0 14-15 37.25216463302213 39.0 36.0 40.0 32.0 41.0 16-17 37.204775741703564 39.0 36.0 40.0 31.5 41.0 18-19 37.181088692284675 39.0 36.0 40.0 31.0 41.0 20-21 37.13674555132219 39.0 36.0 40.0 31.0 41.0 22-23 37.0035195385089 39.0 36.0 40.0 31.0 41.0 24-25 36.874631581474844 39.0 36.0 40.0 31.0 41.0 26-27 36.58600262577253 38.0 35.0 40.0 30.0 41.0 28-29 36.38631699814687 38.0 35.0 40.0 30.0 41.0 30-31 36.144906498658386 38.0 34.5 40.0 29.5 41.0 32-33 35.90535663599263 38.0 34.0 40.0 29.0 41.0 34-35 36.11896970585688 38.0 35.0 40.0 30.0 41.0 36-37 36.176086261172614 38.0 35.0 40.0 30.0 41.0 38-39 36.13679636027794 38.0 35.0 40.0 30.0 41.0 40-41 36.03621949640393 38.0 35.0 40.0 29.5 41.0 42-43 35.890832134928004 38.0 34.5 40.0 29.0 41.0 44-45 35.69803680197714 38.0 34.0 40.0 28.0 41.0 46-47 35.479862502819145 38.0 34.0 40.0 27.0 41.0 48-49 35.27376787210378 38.0 34.0 40.0 27.0 41.0 50-51 34.997598258517684 38.0 33.5 40.0 26.5 41.0 52-53 34.77258575994334 38.0 33.0 40.0 26.0 41.0 54-55 34.5805103277242 37.5 33.0 40.0 26.0 41.0 56-57 34.30606671794727 37.0 33.0 40.0 25.0 41.0 58-59 34.05063037406539 37.0 32.5 40.0 25.0 41.0 60-61 33.72098529927547 36.5 32.0 39.5 24.0 41.0 62-63 33.403139907712074 36.0 32.0 39.0 23.0 41.0 64-65 33.077634154937726 36.0 31.0 39.0 23.0 40.0 66-67 32.72915910963844 35.0 31.0 39.0 22.0 40.0 68-69 32.34240069528947 35.0 31.0 38.0 22.0 40.0 70-71 31.91771243074332 35.0 30.5 37.5 21.0 40.0 72-73 31.485077602640523 34.5 30.0 37.0 20.0 39.0 74-75 30.948671377964605 34.0 29.5 36.5 18.0 39.0 76-77 29.6179651034659 32.5 28.5 35.0 17.5 37.5 78-79 30.38093982418815 34.0 29.0 36.0 18.0 38.0 80-81 30.37701317088188 34.0 30.0 35.0 18.0 37.0 82-83 30.100865210057513 34.0 29.0 35.0 18.0 37.0 84-85 29.776413153559673 34.0 29.0 35.0 15.5 36.0 86-87 29.418777060592575 34.0 29.0 35.0 10.5 36.0 88-89 29.06556370499763 34.0 29.0 35.0 7.0 36.0 90-91 28.707427240289057 33.0 29.0 35.0 3.5 35.0 92-93 28.32506455095602 33.0 28.5 35.0 2.0 35.0 94-95 27.87133585963179 33.0 27.0 35.0 2.0 35.0 96-97 27.366628206216618 33.0 26.5 35.0 2.0 35.0 98-99 26.833216494173477 33.0 25.5 35.0 2.0 35.0 100-101 25.580620735370665 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4713221174503097 1101 2 0.5517766375290165 1101 3 0.5231789274746532 1101 4 0.31862852906437666 1101 5 0.5297730075636835 1101 6 0.5212846553806187 1101 7 0.4541498577731886 1101 8 0.5326137912762618 1101 9 0.6440385377647573 1101 10-11 0.5903564945550954 1101 12-13 0.6267284624698846 1101 14-15 0.6942609244980176 1101 16-17 0.7760224547100449 1101 18-19 0.7153087335984409 1101 20-21 0.6603921536033965 1101 22-23 0.7509910804093067 1101 24-25 0.6451865339865961 1101 26-27 0.711296797994244 1101 28-29 0.6824993382778644 1101 30-31 0.6795697258096638 1101 32-33 0.6511644417073725 1101 34-35 0.5372025769579807 1101 36-37 0.6586191385609368 1101 38-39 0.7254161367370173 1101 40-41 0.6897740220659543 1101 42-43 0.5183114137515261 1101 44-45 0.748526522328028 1101 46-47 0.7187549253749452 1101 48-49 0.730188474404585 1101 50-51 0.7032375278791463 1101 52-53 0.6882755571589811 1101 54-55 0.8097400976643812 1101 56-57 1.0580123076824108 1101 58-59 0.943518890901224 1101 60-61 0.7572253208672777 1101 62-63 0.8112469667325755 1101 64-65 0.8244835560923889 1101 66-67 0.9053845543990562 1101 68-69 0.7924540374219049 1101 70-71 0.6385126852038674 1101 72-73 0.7418948409643455 1101 74-75 0.6543949474509958 1101 76-77 0.7115988080152995 1101 78-79 0.7809148570049338 1101 80-81 0.8328073429420968 1101 82-83 0.9677918726174681 1101 84-85 1.121455906749393 1101 86-87 1.0242119422941869 1101 88-89 0.9499534978652164 1101 90-91 0.7791451418526414 1101 92-93 0.8678922518609795 1101 94-95 0.9874697057451236 1101 96-97 1.0439165984103198 1101 98-99 1.001981281600056 1101 100-101 1.34097416496909 1102 1 0.49086347612016823 1102 2 0.5973391237955958 1102 3 0.5761238684513863 1102 4 0.38727924076734155 1102 5 0.45549801935450773 1102 6 0.5192796889555353 1102 7 0.5207063264351461 1102 8 0.501366224214344 1102 9 0.6948146882232109 1102 10-11 0.6647742652308253 1102 12-13 0.653495440582688 1102 14-15 0.8102574754748133 1102 16-17 0.7635916628745534 1102 18-19 0.8106412048262612 1102 20-21 0.7779918510849271 1102 22-23 0.7743968841107645 1102 24-25 0.7267621400079918 1102 26-27 0.7689198186159487 1102 28-29 0.8881078185343512 1102 30-31 0.8186138467942072 1102 32-33 0.9953654746107645 1102 34-35 0.9520585365027472 1102 36-37 0.9708539577036532 1102 38-39 1.0450272483992151 1102 40-41 1.025988705574342 1102 42-43 0.9014158592761063 1102 44-45 1.0078525470026491 1102 46-47 1.0354157755348083 1102 48-49 1.0230097627593366 1102 50-51 1.1602375359952006 1102 52-53 1.1062685226425941 1102 54-55 0.978351311228181 1102 56-57 0.9958122823723414 1102 58-59 1.0968113493718192 1102 60-61 1.0224529206278845 1102 62-63 1.0124393355959 1102 64-65 0.8628606274526618 1102 66-67 0.950821016351739 1102 68-69 0.9236145372386915 1102 70-71 1.029688398116722 1102 72-73 0.981830405491646 1102 74-75 1.00595280936923 1102 76-77 1.1730535539391624 1102 78-79 1.2405718965262196 1102 80-81 1.1382007026255572 1102 82-83 1.1876498175900068 1102 84-85 1.2036479572829641 1102 86-87 1.2038412279099227 1102 88-89 1.2171253439282808 1102 90-91 1.1498563698750353 1102 92-93 1.287098041674966 1102 94-95 1.2535518961423868 1102 96-97 1.1844944285316465 1102 98-99 1.2797943291520255 1102 100-101 1.36428588253996 1103 1 0.47276628234266127 1103 2 0.49873914848803125 1103 3 0.43548593215786724 1103 4 0.33375657456547003 1103 5 0.3686777875361287 1103 6 0.45632578391973766 1103 7 0.42013731108225727 1103 8 0.4502374531713045 1103 9 0.5601147376080746 1103 10-11 0.5111373965219457 1103 12-13 0.5872057874387622 1103 14-15 0.5934184156755506 1103 16-17 0.6554315397028816 1103 18-19 0.6354551945425939 1103 20-21 0.7290177672468516 1103 22-23 0.754829038017256 1103 24-25 0.6376397018480162 1103 26-27 0.6407388521250326 1103 28-29 0.6904044872345168 1103 30-31 0.8790372312554027 1103 32-33 0.8409646837266322 1103 34-35 0.7240860939852851 1103 36-37 0.7788898016765202 1103 38-39 0.7256451402463568 1103 40-41 0.9272829703934349 1103 42-43 0.834554285787064 1103 44-45 0.8821807675496558 1103 46-47 0.9035708875948885 1103 48-49 0.9253244060989161 1103 50-51 0.9310776779040708 1103 52-53 0.8525510403993621 1103 54-55 0.9225504956516133 1103 56-57 0.9247961160905902 1103 58-59 0.996374420384349 1103 60-61 0.9295381058937693 1103 62-63 0.9254121230811947 1103 64-65 0.8537366823279697 1103 66-67 0.6668344228908722 1103 68-69 0.9191290476748719 1103 70-71 0.8456171967571855 1103 72-73 0.7394673577190467 1103 74-75 0.7383136057728521 1103 76-77 1.015210801168525 1103 78-79 1.0221532472023576 1103 80-81 0.9132031537127538 1103 82-83 0.9714757286681959 1103 84-85 1.0476299027562597 1103 86-87 1.1831390731319367 1103 88-89 1.0867415762986106 1103 90-91 1.0616930330557146 1103 92-93 1.044575673234803 1103 94-95 1.2253448755024188 1103 96-97 1.0733471563193504 1103 98-99 1.3001083951244006 1103 100-101 1.6366397977432356 1104 1 0.376113380296772 1104 2 0.5608110693826163 1104 3 0.4016229222781007 1104 4 0.2669482963266745 1104 5 0.32411024656670406 1104 6 0.39782269093554845 1104 7 0.4395072146878576 1104 8 0.40441721755656346 1104 9 0.5589640309320885 1104 10-11 0.5640099725427916 1104 12-13 0.5256148922757831 1104 14-15 0.5874913225039009 1104 16-17 0.6037183821433345 1104 18-19 0.6864871055117518 1104 20-21 0.6649398075830177 1104 22-23 0.7000173387504915 1104 24-25 0.712116604201519 1104 26-27 0.7917991064370611 1104 28-29 0.6620516064574034 1104 30-31 0.7566463358521034 1104 32-33 0.833946664750556 1104 34-35 0.7168498297670496 1104 36-37 0.6970726944939258 1104 38-39 0.7921376913695113 1104 40-41 0.7170509935751923 1104 42-43 0.7030711403879693 1104 44-45 0.820894559567499 1104 46-47 0.7950631983929313 1104 48-49 0.720211954975035 1104 50-51 0.7847628682295422 1104 52-53 0.8656600242326888 1104 54-55 0.766911071638809 1104 56-57 0.8299674318465406 1104 58-59 0.7713412705392457 1104 60-61 0.746484045360674 1104 62-63 0.6379473907913535 1104 64-65 0.752701376766602 1104 66-67 0.7889469166724581 1104 68-69 0.762016656031463 1104 70-71 0.6201387695408407 1104 72-73 0.7681446773971068 1104 74-75 0.9500548242932645 1104 76-77 1.0646348620380692 1104 78-79 1.2047660107488838 1104 80-81 0.9501282810249805 1104 82-83 1.031519606025487 1104 84-85 1.0762210809020551 1104 86-87 1.0739119857280919 1104 88-89 1.063703485369203 1104 90-91 1.1576116667834206 1104 92-93 1.1169644507730574 1104 94-95 1.1741417328910906 1104 96-97 1.2031291003332107 1104 98-99 1.3345155911792226 1104 100-101 1.651697968436551 1105 1 0.22619170911714548 1105 2 0.3462314472943433 1105 3 0.2792158769659778 1105 4 0.11297297810802576 1105 5 0.200767698972399 1105 6 0.16216425138219392 1105 7 0.2382447427341674 1105 8 0.2315957512075002 1105 9 0.3536826364657344 1105 10-11 0.3393927552692162 1105 12-13 0.2522158952554747 1105 14-15 0.4144258280744708 1105 16-17 0.32652639273843675 1105 18-19 0.3162126390298212 1105 20-21 0.34167580215892457 1105 22-23 0.4344834469506438 1105 24-25 0.390054346951338 1105 26-27 0.31390340096343294 1105 28-29 0.44538218586113487 1105 30-31 0.5380708918894754 1105 32-33 0.6056330510735677 1105 34-35 0.5616189844999795 1105 36-37 0.5996966621565605 1105 38-39 0.5052305593196493 1105 40-41 0.382563903521401 1105 42-43 0.3751419831301561 1105 44-45 0.2838140623770258 1105 46-47 0.4041003446607263 1105 48-49 0.35368324963633313 1105 50-51 0.33355730182911003 1105 52-53 0.4142488327238354 1105 54-55 0.5405351895291588 1105 56-57 0.5430736157696998 1105 58-59 0.5531426465427458 1105 60-61 0.46803204120374176 1105 62-63 0.34526355421969157 1105 64-65 0.3371991710970619 1105 66-67 0.33611628657974535 1105 68-69 0.30162968944101465 1105 70-71 0.2292970838063404 1105 72-73 0.31852893518022185 1105 74-75 0.4635614879445882 1105 76-77 0.4659873381984312 1105 78-79 0.5598209086065431 1105 80-81 0.4774319112535039 1105 82-83 0.5668341550576166 1105 84-85 0.5266922824251061 1105 86-87 0.7071451378219642 1105 88-89 0.618256304831533 1105 90-91 0.5125056694310182 1105 92-93 0.560515539747449 1105 94-95 0.4412951707148416 1105 96-97 0.33601000447747964 1105 98-99 0.6754693885783851 1105 100-101 1.0246195179331963 1106 1 0.005241909412003309 1106 2 0.1118992280103015 1106 3 0.09724326513379822 1106 4 0.028599168123278673 1106 5 0.09984802327349485 1106 6 0.2420726454705715 1106 7 0.16104772194732675 1106 8 0.13632645407970756 1106 9 0.20551869039110926 1106 10-11 0.21548065524730475 1106 12-13 0.07797426073030067 1106 14-15 0.1668531932663555 1106 16-17 6.696349409835989E-4 1106 18-19 0.256780485057007 1106 20-21 0.07190745491593731 1106 22-23 0.17580471224399474 1106 24-25 -0.0019390708682038849 1106 26-27 0.06928732248784542 1106 28-29 0.20794130103227815 1106 30-31 0.23752329872523603 1106 32-33 0.23169276298084895 1106 34-35 0.1564085525736374 1106 36-37 0.2550639899487237 1106 38-39 0.28613691748471837 1106 40-41 0.305448255844631 1106 42-43 0.2591789190762128 1106 44-45 0.13039064776985754 1106 46-47 0.05784074210189516 1106 48-49 0.22982858945682239 1106 50-51 0.14016320281559302 1106 52-53 0.034954938579083716 1106 54-55 0.0633781638918478 1106 56-57 0.3591554952013496 1106 58-59 0.35683985773201954 1106 60-61 0.12419501718325421 1106 62-63 -0.11698145010957006 1106 64-65 0.09642837096005508 1106 66-67 0.020535410217945582 1106 68-69 -0.03627401159949528 1106 70-71 -0.07861450989399543 1106 72-73 0.011423626675082232 1106 74-75 0.04728005824563297 1106 76-77 -0.10360847677720031 1106 78-79 0.05499170704162637 1106 80-81 0.039519062386236925 1106 82-83 0.20934293590759978 1106 84-85 0.2895008354303812 1106 86-87 0.2179044712645002 1106 88-89 0.15780204077419313 1106 90-91 0.2063736471443569 1106 92-93 0.46317362163964404 1106 94-95 0.5100373658103301 1106 96-97 0.349293499883359 1106 98-99 0.3521543438072179 1106 100-101 0.4137069052075084 1107 1 0.08786071434644427 1107 2 0.08981910251917213 1107 3 0.15107395660561096 1107 4 -0.019223456326777466 1107 5 -0.045112592261226325 1107 6 0.08829825064404417 1107 7 0.06482188490957697 1107 8 0.05063127398145184 1107 9 0.223840450605735 1107 10-11 0.06559284663983078 1107 12-13 0.05869165497159656 1107 14-15 0.14383935035099427 1107 16-17 0.03439746214706929 1107 18-19 0.055492618143389905 1107 20-21 0.14929030085510675 1107 22-23 0.14279334563907042 1107 24-25 0.039783591020203346 1107 26-27 0.10303903171396911 1107 28-29 0.04238472321290487 1107 30-31 0.00617654756104713 1107 32-33 0.29321910693573017 1107 34-35 -0.025700274622870722 1107 36-37 0.11409824124816481 1107 38-39 0.08367195294913898 1107 40-41 -0.020168557221140304 1107 42-43 0.10216143867415894 1107 44-45 -0.0745044862028621 1107 46-47 0.023791087285964352 1107 48-49 0.22322795464335599 1107 50-51 0.23133602176884693 1107 52-53 -0.007276742674939385 1107 54-55 0.09473162366231236 1107 56-57 0.018999014641309486 1107 58-59 0.1687490868884325 1107 60-61 0.10298716508421535 1107 62-63 0.11780647600672012 1107 64-65 0.09105252052054169 1107 66-67 0.06129173910782981 1107 68-69 0.21650163560325097 1107 70-71 0.12791364414797002 1107 72-73 0.008964628780098849 1107 74-75 0.12343742421803938 1107 76-77 0.1705884922312002 1107 78-79 0.26820048907096705 1107 80-81 0.28068217395530937 1107 82-83 0.3654318771875076 1107 84-85 0.35154261366240647 1107 86-87 0.19624680247735782 1107 88-89 0.15060350529076771 1107 90-91 0.26403983740723547 1107 92-93 0.3054826528148773 1107 94-95 0.315094310129826 1107 96-97 0.3068476533298963 1107 98-99 0.23007471295987258 1107 100-101 0.5949518834138132 1108 1 0.223566606574078 1108 2 0.3169356600705129 1108 3 0.3256808616472995 1108 4 0.060436327680832846 1108 5 0.198421625186036 1108 6 0.13937541476469306 1108 7 0.2606027589513147 1108 8 0.31764487142982745 1108 9 0.3389767048724224 1108 10-11 0.13728441398356495 1108 12-13 0.17792393072561197 1108 14-15 0.3506772441454089 1108 16-17 0.41216031817079823 1108 18-19 0.3269267852297304 1108 20-21 0.4456363363315816 1108 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-0.74741195868285 2316 74-75 -0.4007881756265661 2316 76-77 -0.9220332137064595 2316 78-79 -0.7878045741607274 2316 80-81 -0.8528775319417967 2316 82-83 -1.100668591246805 2316 84-85 -1.2201211126157006 2316 86-87 -1.1232792986741416 2316 88-89 -1.49191472671923 2316 90-91 -1.0915189911761196 2316 92-93 -1.2318229266657994 2316 94-95 -0.9031981085772358 2316 96-97 -0.9180775562061427 2316 98-99 -0.7074085705843061 2316 100-101 -0.8524104653109319 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 43.0 5 93.0 6 208.0 7 508.0 8 1193.0 9 1995.0 10 3136.0 11 4756.0 12 6516.0 13 8395.0 14 9755.0 15 11698.0 16 13859.0 17 15652.0 18 14674.0 19 13719.0 20 14256.0 21 15316.0 22 16828.0 23 19927.0 24 24214.0 25 30607.0 26 38918.0 27 49032.0 28 61645.0 29 75299.0 30 91224.0 31 108950.0 32 128841.0 33 154830.0 34 188033.0 35 231897.0 36 295981.0 37 363003.0 38 277786.0 39 39453.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.58314214036066 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22.27106599333009 27.50578193053451 28.39886187939112 21.824290196744283 20-21 22.867181530751637 26.77595522980655 28.33654051467543 22.020322724766388 22-23 22.62246729628338 26.641628065072354 28.20836650029767 22.527538138346596 24-25 22.56492591181623 26.809038207451202 28.146713834189192 22.479322046543377 26-27 22.51049693908506 26.939095778606504 28.061606339861783 22.48880094244665 28-29 22.558945368784126 27.016563937510547 28.058297822439172 22.366192871266158 30-31 22.804058110217312 26.811484829254066 27.980678524261187 22.40377853626743 32-33 22.60877471294832 26.909199763436735 27.901598946358554 22.58042657725639 34-35 22.777464137638397 26.90836564374716 27.94720182131889 22.366968397295555 36-37 22.85439321789136 26.78288899922564 27.870785086015502 22.491932696867494 38-39 22.639153624727008 26.906789426440504 27.898685026036002 22.555371922796493 40-41 22.788041956572727 26.783975446580747 27.959550145547958 22.46843245129857 42-43 22.724863629678197 26.909967072157816 27.732391280017872 22.63277801814612 44-45 22.70761598065238 26.926764633994694 27.70660829220392 22.659011093149005 46-47 22.837798376470094 26.88952646972937 27.548037609360577 22.72463754443996 48-49 22.811577988862485 27.022954479329904 27.403395798979304 22.762071732828307 50-51 22.821480339055636 27.05449979800731 27.363620568161096 22.76039929477596 52-53 22.91214181747829 27.02653543871179 27.33443609886968 22.72688664494024 54-55 22.74626005411656 27.005553819904936 27.425505029658183 22.82268109632032 56-57 22.80038970773635 27.105892614249377 27.272894900694855 22.82082277731942 58-59 22.79483456662419 27.015341782281656 27.38771992686241 22.80210372423175 60-61 22.79259172666307 27.125417061250488 27.407329553193478 22.674661658892965 62-63 22.81933172165555 27.05022194545502 27.36583383295917 22.76461249993026 64-65 22.608251904861845 26.953113767237962 27.444452885222443 22.994181442677746 66-67 22.606939810169013 27.094759260419714 27.342992846786636 22.955308082624637 68-69 22.69369078789666 27.038134595695084 27.349969537561076 22.91820507884718 70-71 22.670061834424846 26.886484252574217 27.344497161049524 23.09895675195141 72-73 22.65989181257381 26.9956482610743 27.264261142950925 23.080198783400963 74-75 22.62948924596591 27.112229564369812 27.196142210123714 23.062138979540574 76-77 22.591856798180224 27.029270389015647 27.184014140629348 23.19485867217478 78-79 22.556686301423316 27.066655689384067 27.153702109996658 23.222955899195956 80-81 22.593271082936095 27.150013023917918 27.09896784110606 23.15774805203993 82-83 22.68493387975471 27.048158314703386 27.202857650027912 23.064050155513993 84-85 22.63217403160703 27.067538608374857 27.029656994528068 23.270630365490042 86-87 22.53765222320267 27.15312061317191 27.099010147213054 23.210217016412365 88-89 22.535215796846238 26.968720937561503 27.07987907024208 23.41618419535018 90-91 22.575711220116276 26.99873912005974 27.08960137455424 23.335948285269744 92-93 22.586363389774977 26.875848184582118 27.14630233871118 23.391486086931724 94-95 22.42309305155486 26.793988081813403 27.229386073610108 23.553532793021624 96-97 22.413695521865858 26.735994093298103 27.23488658669294 23.61542379814309 98-99 22.329163997588612 26.728083331832647 27.15725822011726 23.785494450461485 100-101 22.333301604533958 26.717214074209373 27.108700294048795 23.840784027207874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 179.0 1 128.0 2 117.0 3 161.5 4 145.5 5 148.0 6 241.0 7 418.0 8 610.5 9 737.0 10 703.5 11 658.5 12 715.5 13 803.5 14 917.0 15 1054.5 16 1221.0 17 1354.0 18 1547.5 19 1807.0 20 2102.5 21 2533.5 22 3136.0 23 3987.0 24 5127.5 25 6891.0 26 9210.0 27 11976.0 28 15567.0 29 20505.5 30 26337.5 31 32717.0 32 40051.5 33 45864.5 34 50265.5 35 54397.0 36 59454.0 37 64716.0 38 68131.0 39 70145.5 40 71836.5 41 73617.0 42 75616.0 43 78550.0 44 81336.0 45 84164.5 46 86806.0 47 88587.0 48 89780.5 49 89477.0 50 87554.0 51 85990.0 52 84754.5 53 82985.0 54 80138.0 55 74710.5 56 67364.0 57 59849.0 58 53361.0 59 46838.5 60 39351.0 61 32341.0 62 26356.0 63 20638.0 64 15495.5 65 11588.0 66 8662.0 67 6790.5 68 5280.5 69 3969.5 70 3063.5 71 2351.0 72 1774.0 73 1337.5 74 1032.5 75 810.0 76 543.5 77 338.0 78 220.0 79 133.5 80 77.0 81 45.0 82 30.5 83 14.0 84 7.5 85 5.5 86 2.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01783673045870411 2 0.0028298658900828637 3 0.002786989134172517 4 0.030914141011359766 5 0.0015006864568621245 6 8.575351182069285E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.143837795517321E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.143837795517321E-5 24-25 4.930826929689838E-4 26-27 0.0016507551025483373 28-29 2.143837795517321E-5 30-31 0.006260006362910577 32-33 0.023282078459318106 34-35 0.014127891072459145 36-37 5.14521070924157E-4 38-39 6.217129607000231E-4 40-41 0.0019294540159655888 42-43 0.008446720914338244 44-45 0.008403844158427898 46-47 0.00801795335523478 48-49 0.010183229528707274 50-51 0.18057545751642395 52-53 0.04960840658827081 54-55 0.01918734826988002 56-57 0.01123371004851076 58-59 0.021288309309486997 60-61 0.05245971085630884 62-63 0.09385721868774831 64-65 0.09891667588516918 66-67 0.10307572120847278 68-69 0.10065318449953822 70-71 0.08967673498648954 72-73 0.06810972676358529 74-75 0.23331386728615003 76-77 0.2780772004565517 78-79 0.007589185796131315 80-81 7.932199843414088E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.2863026773103926E-4 90-91 0.007053226347251987 92-93 0.01918734826988002 94-95 0.0013720561891310855 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2332266.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.36601516096302 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24268095480144 53.10510315008832 2 10.918827479055917 14.274404850718172 3 3.2962706105971717 6.463922241207946 4 1.4433763162281499 3.7739103267817673 5 0.8208122959900169 2.6826614491994154 6 0.5271938182679693 2.0676335470602036 7 0.3404058306668099 1.5575680881772835 8 0.24887421464318762 1.3014332550031493 9 0.19824473322131556 1.1662621413593022 >10 0.916573675034595 10.26877422414773 >50 0.03462156614041349 1.527145563387324 >100 0.011461057252533583 1.3267266651810021 >500 4.602136703256527E-4 0.17035195856000668 >1k 1.972344301377832E-4 0.31410253912834196 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3041 0.13038821472336345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2731 0.11709642039115606 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.2876755910346417E-5 2 0.0 0.0 0.0 0.0 4.2876755910346417E-5 3 0.0 0.0 0.0 0.0 4.2876755910346417E-5 4 0.0 0.0 0.0 0.0 4.2876755910346417E-5 5 0.0 0.0 0.0 0.0 4.2876755910346417E-5 6 0.0 0.0 0.0 0.0 4.2876755910346417E-5 7 0.0 0.0 0.0 0.0 4.2876755910346417E-5 8 0.0 0.0 0.0 0.0 4.2876755910346417E-5 9 0.0 0.0 0.0 0.0 4.2876755910346417E-5 10-11 0.0 0.0 0.0 0.0 4.2876755910346417E-5 12-13 4.2876755910346417E-5 0.0 0.0 0.0 4.502059370586374E-4 14-15 4.2876755910346417E-5 0.0 0.0 0.0 6.002745827448498E-4 16-17 6.431513386551962E-5 0.0 0.0 0.0 6.860280945655427E-4 18-19 8.575351182069283E-5 0.0 0.0 0.0 7.289048504758891E-4 20-21 8.575351182069283E-5 0.0 0.0 2.1438377955173208E-5 8.360967402517552E-4 22-23 8.575351182069283E-5 0.0 0.0 8.575351182069283E-5 9.218502520724481E-4 24-25 8.575351182069283E-5 0.0 0.0 2.5726053546207847E-4 9.432886300276212E-4 26-27 8.575351182069283E-5 0.0 0.0 4.287675591034642E-4 9.432886300276212E-4 28-29 8.575351182069283E-5 0.0 0.0 0.0014792480789069516 9.432886300276212E-4 30-31 8.575351182069283E-5 0.0 0.0 0.004759319906048453 9.432886300276212E-4 32-33 8.575351182069283E-5 0.0 0.0 0.014771042411114342 9.861653859379676E-4 34-35 8.575351182069283E-5 0.0 0.0 0.03039961994043561 0.001029042141848314 36-37 8.575351182069283E-5 0.0 0.0 0.056061358352777944 0.001029042141848314 38-39 8.575351182069283E-5 0.0 0.0 0.09383578030979314 0.0010933572757138338 40-41 8.575351182069283E-5 0.0 0.0 0.13120287308566003 0.001114795653669007 42-43 8.575351182069283E-5 0.0 0.0 0.17217161335799605 0.0011362340316241801 44-45 8.575351182069283E-5 0.0 0.0 0.22555317446637735 0.0012005491654896996 46-47 8.575351182069283E-5 0.0 0.0 0.2886891975443624 0.0012648642993552193 48-49 8.575351182069283E-5 0.0 0.0 0.3542263189533269 0.0012863026773103925 50-51 8.575351182069283E-5 0.0 0.0 0.4227862516539709 0.0013720561891310853 52-53 8.575351182069283E-5 0.0 0.0 0.49353289890604246 0.0014792480789069516 54-55 8.575351182069283E-5 0.0 0.0 0.5724261297810799 0.0015864399686828176 56-57 8.575351182069283E-5 0.0 0.0 0.6651899911931143 0.0016078783466379907 58-59 8.575351182069283E-5 0.0 0.0 0.7679441367322595 0.001629316724593164 60-61 8.575351182069283E-5 0.0 0.0 0.8806885663985154 0.0016721934805035102 62-63 8.575351182069283E-5 0.0 0.0 0.9958340943957508 0.0017150702364138568 64-65 8.575351182069283E-5 0.0 0.0 1.1183115476536554 0.0017150702364138568 66-67 8.575351182069283E-5 0.0 0.0 1.2601049794491708 0.001757946992324203 68-69 8.575351182069283E-5 0.0 0.0 1.422264870302101 0.0018222621261897228 70-71 8.575351182069283E-5 0.0 0.0 1.599388748967742 0.001843700504144896 72-73 8.575351182069283E-5 0.0 0.0 1.7869745560755077 0.001950892393920762 74-75 8.575351182069283E-5 0.0 0.0 1.980091464695708 0.002100961039606974 76-77 8.575351182069283E-5 0.0 0.0 2.2000920992716955 0.002229591307338014 78-79 8.575351182069283E-5 0.0 0.0 2.4484985846382874 0.002229591307338014 80-81 8.575351182069283E-5 0.0 0.0 2.7203372171098836 0.0022724680632483603 82-83 8.575351182069283E-5 0.0 0.0 3.005424767157777 0.0024010983309793992 84-85 8.575351182069283E-5 0.0 0.0 3.298616024072726 0.0024010983309793992 86-87 8.575351182069283E-5 0.0 0.0 3.622635668487214 0.0025511669766656118 88-89 8.575351182069283E-5 0.0 0.0 3.9774408236453302 0.0027012356223518243 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2715 0.0 49.840805 1 GTATCAA 4450 0.0 37.663845 1 ATCAACG 5280 0.0 30.480347 3 TCAACGC 5280 0.0 30.38913 4 CAACGCA 5400 0.0 29.790224 5 TATCAAC 5620 0.0 28.974232 2 AACGCAG 5815 0.0 27.745192 6 GTACATG 6140 0.0 24.435919 1 GTGGTAT 1340 0.0 23.739962 1 ACGCAGA 6840 0.0 23.656845 7 TACATGG 6280 0.0 23.358929 2 CGCAGAG 7005 0.0 23.099617 8 ACATGGG 6435 0.0 21.984766 3 TATACCG 185 6.612703E-6 20.520414 5 GCAGAGT 8125 0.0 19.331392 9 CATGGGG 5020 0.0 19.291319 4 TGGTATC 1510 0.0 19.178162 2 CAGAGTA 7795 0.0 17.988705 10-11 GAGTACT 5130 0.0 17.574978 12-13 AGAGTAC 7400 0.0 17.570229 10-11 >>END_MODULE