FastQCFastQC Report
Sat 18 Jun 2016
SRR1930937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939311
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54160.18426086929896157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.052.555281
GTATCAA41850.042.346881
ATCAACG50200.034.890043
TCAACGC51600.034.2282454
TATCAAC52300.033.396672
CAACGCA53900.032.4525955
AACGCAG59600.030.2218556
GTACATG64700.028.2472741
TACATGG66350.026.7799152
ACATGGG67250.025.9694753
ACGCAGA69350.025.7234737
GTGGTAT11550.024.8466071
CGCAGAG73550.024.245168
CATGGGG50750.022.9035684
TATCACG2356.1419996E-821.4194132
GCAGAGT82950.020.4998669
AGAGTAC72800.019.10371810-11
GTATAGC9650.018.7823281
TGGTATC13800.018.2375432
CAGAGTA76850.018.12572110-11
Produced by FastQC (version 0.11.5)