##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930937_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2939311 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.76113483738196 31.0 25.0 31.0 2.0 34.0 2 23.411255903169145 31.0 10.0 31.0 2.0 34.0 3 23.992000846456875 31.0 16.0 31.0 2.0 34.0 4 26.03307373734865 35.0 10.0 37.0 2.0 37.0 5 25.972909977882573 35.0 10.0 37.0 2.0 37.0 6 26.915860213499013 35.0 17.0 37.0 2.0 37.0 7 27.111571045051033 35.0 17.0 37.0 2.0 37.0 8 28.15789584701993 35.0 26.0 37.0 2.0 37.0 9 29.648179454300685 35.0 27.0 39.0 2.0 39.0 10-11 30.150632580220332 36.0 29.0 39.0 2.0 39.0 12-13 30.240253583237703 37.0 30.0 39.0 2.0 39.0 14-15 30.637114446208653 37.0 28.5 40.0 2.0 41.0 16-17 29.622622274403763 36.5 26.0 39.5 2.0 41.0 18-19 28.84128134110341 36.0 23.5 39.0 2.0 41.0 20-21 28.3498192263425 36.0 18.0 39.0 2.0 40.5 22-23 28.3354012215788 36.0 18.5 39.0 2.0 40.0 24-25 28.07655314459749 35.0 18.0 39.0 2.0 40.0 26-27 27.846093012954398 35.0 17.0 39.0 2.0 40.0 28-29 27.919830531713046 35.0 17.5 39.0 2.0 40.0 30-31 27.9633257589959 35.0 17.5 39.0 2.0 40.0 32-33 27.833068361939244 35.0 18.0 39.0 2.0 40.0 34-35 24.4843675949908 32.0 7.0 38.0 2.0 40.0 36-37 22.739628947055962 25.0 6.0 37.5 2.0 40.0 38-39 21.721048572267446 26.0 2.0 37.0 2.0 40.0 40-41 23.546184462957477 30.0 5.5 37.5 2.0 40.0 42-43 23.559904174821924 30.0 5.0 38.0 2.0 40.0 44-45 21.234735283200724 22.5 2.0 37.0 2.0 39.5 46-47 22.556667361840923 27.0 4.5 36.0 2.0 39.0 48-49 22.005660510235224 28.5 2.0 37.0 2.0 39.0 50-51 19.39884432099904 14.5 4.5 35.0 2.0 38.5 52-53 23.108058317068185 28.0 4.0 36.0 2.0 39.0 54-55 25.230045748816643 32.5 2.0 37.5 2.0 39.5 56-57 25.562076792826616 33.0 2.0 38.0 2.0 40.0 58-59 25.160170359652312 33.0 2.0 38.0 2.0 40.0 60-61 24.927827473853565 32.0 2.0 38.0 2.0 40.0 62-63 24.509477901453778 32.0 2.0 37.0 2.0 40.0 64-65 24.0384612244162 31.0 2.0 36.5 2.0 39.0 66-67 23.652098740146926 31.0 2.0 36.0 2.0 39.0 68-69 23.22460807992077 31.0 2.0 35.5 2.0 39.0 70-71 22.635426465590065 30.0 2.0 35.0 2.0 38.0 72-73 22.235850850760603 29.0 2.0 35.0 2.0 37.5 74-75 21.719348003664805 29.0 2.0 34.5 2.0 37.0 76-77 21.18506939211264 28.5 2.0 34.0 2.0 36.0 78-79 20.743101189360363 28.0 2.0 34.0 2.0 36.0 80-81 20.29749216738208 27.0 2.0 34.0 2.0 35.0 82-83 19.87058140496191 26.0 2.0 34.0 2.0 35.0 84-85 19.392613268891928 26.0 2.0 34.0 2.0 35.0 86-87 18.85615370404833 25.0 2.0 33.0 2.0 35.0 88-89 18.326102443735962 24.5 2.0 33.0 2.0 35.0 90-91 17.773389239859274 22.0 2.0 32.5 2.0 35.0 92-93 17.153663222435462 19.5 2.0 32.0 2.0 34.0 94-95 16.47958756320784 15.0 2.0 32.0 2.0 34.0 96-97 15.707062301335245 4.5 2.0 32.0 2.0 34.0 98-99 14.804268755500864 2.0 2.0 31.0 2.0 34.0 100-101 13.364345759941699 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.544007671365925 1101 2 7.996623759291687 1101 3 7.490205203997576 1101 4 9.332479148955606 1101 5 9.420586471914607 1101 6 8.44572625715271 1101 7 8.216766816455038 1101 8 7.06988745099115 1101 9 7.186005846853607 1101 10-11 6.508376501852084 1101 12-13 6.203226259843227 1101 14-15 6.967375873332298 1101 16-17 7.849021153370387 1101 18-19 8.587851580588758 1101 20-21 8.988300387415688 1101 22-23 8.814932347324362 1101 24-25 8.949926212052944 1101 26-27 8.92395482274664 1101 28-29 8.7384399952964 1101 30-31 8.690416546562528 1101 32-33 8.620866657945658 1101 34-35 11.44045285498408 1101 36-37 4.77169857376477 1101 38-39 8.656458865849153 1101 40-41 10.497484698604072 1101 42-43 10.721687161734483 1101 44-45 4.390307890388446 1101 46-47 6.135858562313405 1101 48-49 9.97721353143854 1101 50-51 -5.895799525399202 1101 52-53 5.638561349978133 1101 54-55 8.249366671527017 1101 56-57 8.718262156265805 1101 58-59 9.100406722632073 1101 60-61 8.861811661886932 1101 62-63 9.049917493352783 1101 64-65 8.920426152019939 1101 66-67 9.013673870660924 1101 68-69 8.98252322260901 1101 70-71 8.991198267614866 1101 72-73 8.844102395067999 1101 74-75 8.834316933708887 1101 76-77 8.819015272845409 1101 78-79 9.025126621987784 1101 80-81 8.809063553692493 1101 82-83 8.807347013224803 1101 84-85 8.975710940873643 1101 86-87 8.644429013397165 1101 88-89 8.727921830370931 1101 90-91 8.739127404107752 1101 92-93 8.816986861546244 1101 94-95 8.883605372531136 1101 96-97 8.984642058890064 1101 98-99 9.089299256065358 1101 100-101 8.677967582255851 1102 1 6.420520853136789 1102 2 7.71138075951394 1102 3 7.466302071937925 1102 4 9.088185022868615 1102 5 9.167586609766868 1102 6 8.296163980283477 1102 7 7.997438353901625 1102 8 6.90406907275268 1102 9 6.7987107614274045 1102 10-11 6.155324368377336 1102 12-13 5.806882045558922 1102 14-15 6.300741916100261 1102 16-17 7.365750397461738 1102 18-19 7.948521880178294 1102 20-21 8.426471847496199 1102 22-23 8.315118307212163 1102 24-25 8.340232497272083 1102 26-27 8.419771203533973 1102 28-29 8.210653930753413 1102 30-31 7.817344776173751 1102 32-33 7.695769387701777 1102 34-35 10.581696394917628 1102 36-37 -0.09310960059302076 1102 38-39 2.8855873300905586 1102 40-41 8.562214606636921 1102 42-43 9.90484606037931 1102 44-45 0.32749041522295386 1102 46-47 0.6882548566195439 1102 48-49 7.936545651296861 1102 50-51 -7.11343004195132 1102 52-53 4.158636859257843 1102 54-55 7.1746524899052595 1102 56-57 7.744190928846148 1102 58-59 8.038183868977725 1102 60-61 8.085584593892008 1102 62-63 8.106071077652263 1102 64-65 8.122293909501238 1102 66-67 8.149945726574366 1102 68-69 8.074264746804896 1102 70-71 8.122959259926088 1102 72-73 8.061247559126258 1102 74-75 7.9984169413048285 1102 76-77 7.933694681937471 1102 78-79 7.852847473971028 1102 80-81 7.784719125596514 1102 82-83 7.65085324330304 1102 84-85 7.651654427072941 1102 86-87 7.722118834554955 1102 88-89 7.6406264073473515 1102 90-91 7.70307622434559 1102 92-93 7.875462328063616 1102 94-95 7.594319250315259 1102 96-97 7.636040809217111 1102 98-99 7.443633077977113 1102 100-101 7.16599339720185 1103 1 6.436390655063494 1103 2 7.948363085579874 1103 3 7.381323558277266 1103 4 9.209543968029273 1103 5 9.244771303624628 1103 6 8.32498764822741 1103 7 8.0525482237645 1103 8 7.029669983740217 1103 9 6.987778910942399 1103 10-11 6.328169458935818 1103 12-13 6.09037610391421 1103 14-15 6.813206652736749 1103 16-17 7.631623718105516 1103 18-19 8.238727212558526 1103 20-21 8.670689542487157 1103 22-23 8.598412713006145 1103 24-25 8.70796514126934 1103 26-27 8.530629410318173 1103 28-29 8.322254780786796 1103 30-31 8.219124969952556 1103 32-33 8.169925934593525 1103 34-35 11.20671347593401 1103 36-37 -3.9900335214966915 1103 38-39 -2.19331117293083 1103 40-41 7.763330178483859 1103 42-43 10.071827416720065 1103 44-45 0.3323776638909628 1103 46-47 -0.1805892032596006 1103 48-49 8.296517834037413 1103 50-51 -7.457846558310585 1103 52-53 4.368852221367074 1103 54-55 7.69028423815352 1103 56-57 8.218170043736418 1103 58-59 8.57359601603239 1103 60-61 8.58482172494621 1103 62-63 8.359220218614286 1103 64-65 8.421563567908617 1103 66-67 8.510189875668225 1103 68-69 8.420680504007876 1103 70-71 8.41439060199803 1103 72-73 8.335493874863232 1103 74-75 8.234770663802731 1103 76-77 8.29497754523116 1103 78-79 8.029726067149149 1103 80-81 8.00975964625582 1103 82-83 7.946130737089874 1103 84-85 8.145293694847311 1103 86-87 8.150312846086372 1103 88-89 8.127619012500194 1103 90-91 8.220625479458121 1103 92-93 8.374840263213937 1103 94-95 8.248969858332948 1103 96-97 8.230000917493854 1103 98-99 8.024248604108434 1103 100-101 7.679661922970217 1104 1 6.292362189865091 1104 2 7.689722810758447 1104 3 7.122788929991891 1104 4 9.00137241592845 1104 5 9.093049472549932 1104 6 8.193229501554914 1104 7 7.972070778023255 1104 8 6.8518247326158495 1104 9 6.814884045842813 1104 10-11 6.047178558262388 1104 12-13 5.7161845313160455 1104 14-15 6.2957730592448335 1104 16-17 7.263397185121619 1104 18-19 7.99101002354794 1104 20-21 8.2011451088627 1104 22-23 8.070015658214519 1104 24-25 8.189034143777732 1104 26-27 8.389749754489689 1104 28-29 8.097220158406135 1104 30-31 7.855940745055012 1104 32-33 7.5630095654766585 1104 34-35 10.625180522353695 1104 36-37 -4.298214201968904 1104 38-39 -2.605066472572812 1104 40-41 6.973821007183584 1104 42-43 9.33310630155226 1104 44-45 -0.21814691424901156 1104 46-47 -0.9099208628453965 1104 48-49 7.803892504849511 1104 50-51 -7.616502759905877 1104 52-53 3.707692260702057 1104 54-55 6.646289477711033 1104 56-57 7.192515490035586 1104 58-59 7.529917716437325 1104 60-61 7.393404085677105 1104 62-63 7.439656810105937 1104 64-65 7.535185300326685 1104 66-67 7.274462770565243 1104 68-69 7.23528551522843 1104 70-71 7.4573496433785955 1104 72-73 7.329614693007883 1104 74-75 7.31672732063565 1104 76-77 7.356899022725091 1104 78-79 7.326713481167712 1104 80-81 7.069243274074882 1104 82-83 7.252106724366328 1104 84-85 7.271441563611969 1104 86-87 6.9600617871474135 1104 88-89 7.125438097754397 1104 90-91 6.978243600105188 1104 92-93 6.930947670312808 1104 94-95 7.006820816285305 1104 96-97 6.945487933662243 1104 98-99 6.6505010023007625 1104 100-101 6.347640553644295 1105 1 5.707052451339113 1105 2 7.168844114442052 1105 3 6.7675695587208935 1105 4 8.579371279854563 1105 5 8.698570021418345 1105 6 7.671402938234319 1105 7 7.460732245086586 1105 8 6.283614800572199 1105 9 6.152062265647952 1105 10-11 5.491290431414683 1105 12-13 5.181280074260293 1105 14-15 5.683627690236673 1105 16-17 6.540630736507843 1105 18-19 7.386743721369054 1105 20-21 7.680557574183407 1105 22-23 7.494407270150564 1105 24-25 7.473828580556216 1105 26-27 7.558272401895895 1105 28-29 7.387414983586471 1105 30-31 7.233019134463749 1105 32-33 7.176833112517972 1105 34-35 9.913238143474608 1105 36-37 -4.715854590793143 1105 38-39 -2.983885309783485 1105 40-41 6.15157805942901 1105 42-43 8.452900292659326 1105 44-45 -4.226961425185973 1105 46-47 -5.395883145068137 1105 48-49 5.941396577230275 1105 50-51 -12.219839674139134 1105 52-53 0.5368288290197576 1105 54-55 5.524864205005162 1105 56-57 6.614557932240388 1105 58-59 7.031903050586841 1105 60-61 6.752032455038389 1105 62-63 6.796217388342079 1105 64-65 6.729073483698578 1105 66-67 6.525246422406113 1105 68-69 6.469502302847527 1105 70-71 6.584756074898468 1105 72-73 6.281331242262652 1105 74-75 6.3624508782674845 1105 76-77 6.380418826840863 1105 78-79 6.288412914776803 1105 80-81 6.098342977447167 1105 82-83 6.005426510531009 1105 84-85 6.07687276079719 1105 86-87 5.932351740036683 1105 88-89 5.826320527151193 1105 90-91 5.822107667529217 1105 92-93 5.883306328031264 1105 94-95 5.775659905923028 1105 96-97 5.849029111371465 1105 98-99 5.720418772042995 1105 100-101 5.538891977230589 1106 1 5.734929859569192 1106 2 7.179656651132536 1106 3 6.6665087014348465 1106 4 8.143559157225994 1106 5 8.417050862006963 1106 6 7.469417404023911 1106 7 7.124771612252761 1106 8 6.089798247787893 1106 9 5.969005494999603 1106 10-11 5.339262540204388 1106 12-13 5.176835503041339 1106 14-15 5.548707917032186 1106 16-17 6.4395327670061135 1106 18-19 7.168406728955286 1106 20-21 7.575947132830219 1106 22-23 7.2059712832018015 1106 24-25 7.438803390256425 1106 26-27 7.391513414758808 1106 28-29 7.413078694157814 1106 30-31 6.791228264559546 1106 32-33 6.888867009159529 1106 34-35 9.937528241996802 1106 36-37 -0.7493086461768357 1106 38-39 0.7081309938275631 1106 40-41 7.226948982222719 1106 42-43 8.697466420186718 1106 44-45 -4.298382350709424 1106 46-47 -5.35891585105167 1106 48-49 5.7570368917701025 1106 50-51 -12.13540094886093 1106 52-53 0.43692228432653124 1106 54-55 5.39359277730129 1106 56-57 6.245074540416976 1106 58-59 6.602732020934489 1106 60-61 6.597328036967134 1106 62-63 6.7868891869227355 1106 64-65 6.673040785689569 1106 66-67 6.406314499705033 1106 68-69 6.297862445922785 1106 70-71 6.385203691159383 1106 72-73 6.329907145331674 1106 74-75 6.2343640467244335 1106 76-77 6.13768009254531 1106 78-79 5.980182928229208 1106 80-81 5.858235683271207 1106 82-83 5.981672852217351 1106 84-85 5.825317574932466 1106 86-87 5.751259003050649 1106 88-89 5.538729839924972 1106 90-91 5.469916421227403 1106 92-93 5.618223279480514 1106 94-95 5.377392770354252 1106 96-97 5.214947890213946 1106 98-99 5.192971681487352 1106 100-101 4.8743400934329255 1107 1 5.6259545856849265 1107 2 7.205768372826981 1107 3 6.755533859140993 1107 4 8.023337540568605 1107 5 8.429059513889147 1107 6 6.882003985576933 1107 7 7.0060163992930065 1107 8 6.130523118938083 1107 9 6.073621103516995 1107 10-11 5.230267794566714 1107 12-13 4.934861930426251 1107 14-15 5.3838160906519725 1107 16-17 6.380867184333965 1107 18-19 6.827303061942342 1107 20-21 7.362911998359696 1107 22-23 7.203961025401814 1107 24-25 7.3521229227336065 1107 26-27 7.389516455965651 1107 28-29 7.317094743298899 1107 30-31 7.1495514856291855 1107 32-33 6.916113462339499 1107 34-35 9.641568028610479 1107 36-37 -0.9414454703740027 1107 38-39 0.5800927227988204 1107 40-41 7.417341980421082 1107 42-43 8.719409681103198 1107 44-45 -4.183030328507609 1107 46-47 -5.264174177986423 1107 48-49 5.919038869056404 1107 50-51 -12.08171165380866 1107 52-53 0.7192376665098763 1107 54-55 5.790092177591358 1107 56-57 6.735533481615292 1107 58-59 7.030570555935967 1107 60-61 6.997239460563083 1107 62-63 6.856303328032727 1107 64-65 6.803244084846966 1107 66-67 6.810554474010345 1107 68-69 6.6180866220116705 1107 70-71 6.799756719067222 1107 72-73 6.655512099588112 1107 74-75 6.68741052298947 1107 76-77 6.558642739900815 1107 78-79 6.485014482506724 1107 80-81 6.334203675321916 1107 82-83 6.106532459871559 1107 84-85 6.196572047664485 1107 86-87 6.162908882857739 1107 88-89 6.19554204289291 1107 90-91 6.088036192584855 1107 92-93 6.042253832066795 1107 94-95 5.994411885794648 1107 96-97 6.004344918262305 1107 98-99 5.820561550153384 1107 100-101 5.37411355865226 1108 1 5.751215319602622 1108 2 6.505255818971687 1108 3 6.652982479950186 1108 4 7.979454511796263 1108 5 8.240638774028799 1108 6 7.296405510857969 1108 7 7.479055076062185 1108 8 6.204184288650357 1108 9 4.686874706250617 1108 10-11 5.289972642781443 1108 12-13 4.782116176947326 1108 14-15 5.547736261008037 1108 16-17 5.922474672077243 1108 18-19 6.843371253890044 1108 20-21 7.701531569498211 1108 22-23 7.646031049667645 1108 24-25 7.41070683795175 1108 26-27 7.6130305450064135 1108 28-29 7.465433276453954 1108 30-31 7.114302389407722 1108 32-33 6.985508302135468 1108 34-35 10.013290655263276 1108 36-37 -4.642878640284369 1108 38-39 -2.939186730477065 1108 40-41 6.028478017661708 1108 42-43 8.451122155723109 1108 44-45 -4.225972932380241 1108 46-47 -5.377483740706179 1108 48-49 6.123138061845989 1108 50-51 -12.192196227670891 1108 52-53 0.5894954291008752 1108 54-55 5.584207217456662 1108 56-57 6.720433199339254 1108 58-59 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-14.189210161449445 2313 96-97 -13.184388757165035 2313 98-99 -11.975964585949066 2313 100-101 -10.467171850530564 2314 1 -22.50821864266153 2314 2 -21.120215879141522 2314 3 -21.667143306213337 2314 4 -23.68626875864399 2314 5 -23.606531037291955 2314 6 -24.558625935682908 2314 7 -24.741385175510143 2314 8 -25.820972944054045 2314 9 -27.29708755790033 2314 10-11 -27.797448740866358 2314 12-13 -27.911437282172173 2314 14-15 -28.26295556289134 2314 16-17 -27.25677061094163 2314 18-19 -26.453326859317503 2314 20-21 -25.975040757545816 2314 22-23 -25.939503541527326 2314 24-25 -25.688506998526236 2314 26-27 -25.455522914113086 2314 28-29 -25.53959817008693 2314 30-31 -25.568873711221205 2314 32-33 -25.44876842742428 2314 34-35 -22.24284142020842 2314 36-37 -20.45294153336613 2314 38-39 -19.481639560665744 2314 40-41 -21.30013833453955 2314 42-43 -21.311456460629092 2314 44-45 -18.994526391499736 2314 46-47 -20.229056067750204 2314 48-49 -19.773874516770366 2314 50-51 -17.149900630186615 2314 52-53 -20.79682532561611 2314 54-55 -22.85274246807793 2314 56-57 -23.168246045943764 2314 58-59 -22.765177475560094 2314 60-61 -22.531894397704633 2314 62-63 -22.121491947557825 2314 64-65 -21.667622056885318 2314 66-67 -21.270390407879418 2314 68-69 -20.84506298428754 2314 70-71 -20.257881076208367 2314 72-73 -19.86480286104181 2314 74-75 -19.348809949451475 2314 76-77 -18.82025489789446 2314 78-79 -18.389001265524772 2314 80-81 -17.94721364751273 2314 82-83 -17.52023919367175 2314 84-85 -17.060278346036302 2314 86-87 -16.532839148638324 2314 88-89 -16.005087878366933 2314 90-91 -15.44818427985609 2314 92-93 -14.846722545885964 2314 94-95 -14.189210161449445 2314 96-97 -13.424631459690453 2314 98-99 -12.547627426224567 2314 100-101 -11.145099039772939 2315 1 -22.50821864266153 2315 2 -21.120215879141522 2315 3 -21.667143306213337 2315 4 -14.770540228570011 2315 5 -23.606531037291955 2315 6 -24.558625935682908 2315 7 -24.741385175510143 2315 8 -25.820972944054045 2315 9 -19.086730529917055 2315 10-11 -15.993010014587814 2315 12-13 -10.205904956665577 2315 14-15 -9.896438998079503 2315 16-17 -19.667674438538928 2315 18-19 -26.453326859317503 2315 20-21 -25.975040757545816 2315 22-23 -25.939503541527326 2315 24-25 -25.688506998526236 2315 26-27 -25.455522914113086 2315 28-29 -25.53959817008693 2315 30-31 -25.568873711221205 2315 32-33 -25.44876842742428 2315 34-35 -22.24284142020842 2315 36-37 -20.45294153336613 2315 38-39 -19.481639560665744 2315 40-41 -21.30013833453955 2315 42-43 -21.311456460629092 2315 44-45 -18.994526391499736 2315 46-47 -20.229056067750204 2315 48-49 -19.773874516770366 2315 50-51 -17.149900630186615 2315 52-53 -20.79682532561611 2315 54-55 -22.85274246807793 2315 56-57 -23.168246045943764 2315 58-59 -22.765177475560094 2315 60-61 -22.531894397704633 2315 62-63 -22.121491947557825 2315 64-65 -21.667622056885318 2315 66-67 -21.270390407879418 2315 68-69 -20.84506298428754 2315 70-71 -20.257881076208367 2315 72-73 -19.86480286104181 2315 74-75 -19.348809949451475 2315 76-77 -18.82025489789446 2315 78-79 -18.389001265524772 2315 80-81 -17.94721364751273 2315 82-83 -17.52023919367175 2315 84-85 -17.060278346036302 2315 86-87 -16.532839148638324 2315 88-89 -16.005087878366933 2315 90-91 -15.44818427985609 2315 92-93 -14.846722545885964 2315 94-95 -14.189210161449445 2315 96-97 -13.424631459690453 2315 98-99 -12.547627426224567 2315 100-101 -11.145099039772939 2316 1 -22.50821864266153 2316 2 -21.120215879141522 2316 3 -21.667143306213337 2316 4 -23.68626875864399 2316 5 -23.606531037291955 2316 6 -24.558625935682908 2316 7 -24.741385175510143 2316 8 -25.820972944054045 2316 9 -27.29708755790033 2316 10-11 -27.797448740866358 2316 12-13 -27.911437282172173 2316 14-15 -28.26295556289134 2316 16-17 -27.25677061094163 2316 18-19 -26.453326859317503 2316 20-21 -25.975040757545816 2316 22-23 -25.939503541527326 2316 24-25 -25.688506998526236 2316 26-27 -25.455522914113086 2316 28-29 -25.53959817008693 2316 30-31 -25.568873711221205 2316 32-33 -25.44876842742428 2316 34-35 -22.24284142020842 2316 36-37 -20.45294153336613 2316 38-39 -19.481639560665744 2316 40-41 -21.30013833453955 2316 42-43 -21.311456460629092 2316 44-45 -18.994526391499736 2316 46-47 -20.229056067750204 2316 48-49 -19.773874516770366 2316 50-51 -17.149900630186615 2316 52-53 -20.79682532561611 2316 54-55 -22.85274246807793 2316 56-57 -23.168246045943764 2316 58-59 -22.765177475560094 2316 60-61 -22.531894397704633 2316 62-63 -22.121491947557825 2316 64-65 -21.667622056885318 2316 66-67 -21.270390407879418 2316 68-69 -20.84506298428754 2316 70-71 -20.257881076208367 2316 72-73 -19.86480286104181 2316 74-75 -19.348809949451475 2316 76-77 -18.82025489789446 2316 78-79 -18.389001265524772 2316 80-81 -17.94721364751273 2316 82-83 -17.52023919367175 2316 84-85 -17.060278346036302 2316 86-87 -16.532839148638324 2316 88-89 -16.005087878366933 2316 90-91 -15.44818427985609 2316 92-93 -14.846722545885964 2316 94-95 -14.189210161449445 2316 96-97 -13.424631459690453 2316 98-99 -12.547627426224567 2316 100-101 -11.145099039772939 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 377740.0 3 22843.0 4 66229.0 5 22119.0 6 18410.0 7 18671.0 8 22821.0 9 29075.0 10 33518.0 11 33761.0 12 33077.0 13 32430.0 14 30609.0 15 33706.0 16 38054.0 17 41933.0 18 44040.0 19 45999.0 20 49383.0 21 53224.0 22 58722.0 23 64242.0 24 70321.0 25 78094.0 26 87186.0 27 96986.0 28 108739.0 29 123448.0 30 139081.0 31 151312.0 32 153310.0 33 156823.0 34 164822.0 35 161142.0 36 145171.0 37 101772.0 38 29706.0 39 792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.071945071259734 19.311262464447562 13.404683729719277 24.21210873457343 2 15.560192590026595 20.318446494587672 40.544286678729094 23.577074236656635 3 19.030694351044435 25.23050166357883 29.576829311771352 26.161974673605382 4 12.860426412280185 16.478173106152557 38.12061925787905 32.54078122368821 5 13.673633057986903 37.43235851004707 34.86308423802121 14.030924193944823 6 31.353897411711362 36.59103744081873 17.943740600324272 14.111324547145637 7 27.31701744912403 32.113849063958824 22.480125700153692 18.08900778676346 8 25.4434295878728 35.715386347284856 20.672977219986414 18.16820684485593 9 26.255434144048877 16.115439720677543 20.528587236534985 37.10053889873859 10-11 25.121634208151804 26.091944389245462 28.509483932067013 20.276937470535714 12-13 25.350119609801236 23.742386864361357 28.54068990030636 22.366803625531052 14-15 22.665558377260222 25.14032408472064 26.020103743376556 26.174013794642587 16-17 22.661397939715908 28.140041135063708 26.5904720016365 22.608088923583885 18-19 22.584172116221303 27.345229799546754 28.187040401900266 21.883557682331677 20-21 23.229666581713566 26.66824988355566 28.07500932753096 22.027074207199814 22-23 23.038144059915734 26.456451484896753 27.832471346569378 22.672933108618132 24-25 22.82432198269726 26.536337583154012 27.888446775107134 22.750893659041594 26-27 22.727741916852477 26.75838373928343 27.7623660678832 22.751508275980896 28-29 22.773755998831856 26.913984598638518 27.750092529980495 22.562166872549128 30-31 23.02421579610216 26.74869027486832 27.693927728522567 22.533166200506958 32-33 22.851437825016145 26.846723244474518 27.60608145243797 22.695757478071368 34-35 23.149310294468382 26.8642747348923 27.534595595844852 22.451819374794464 36-37 23.557810295452335 26.895046463665707 27.137917617462094 22.409225623419864 38-39 23.373035947231536 26.850634588066534 27.221400186154952 22.554929278546975 40-41 23.368331565166123 26.758773172706025 27.359548953475972 22.513346308651876 42-43 23.363445487414293 26.816607885216726 27.083844014662866 22.73610261270612 44-45 23.545597963372888 26.97462792176235 26.945531349285407 22.534242765579354 46-47 23.049112287265665 26.86900013790771 27.183305063550524 22.898582511276096 48-49 23.46237139494113 26.996019981678625 26.73435720356048 22.80725141981976 50-51 23.734443410211327 27.125478332816215 26.61719584927776 22.522882407694695 52-53 22.9730764196101 27.020474199798368 27.11017226161597 22.89627711897557 54-55 22.702877233403786 26.95266030633045 27.29436550803999 23.050096952225772 56-57 22.75166759011789 27.06078363314975 27.116752723018315 23.070796053714044 58-59 22.84902669018981 27.04374276747736 27.18922651662887 22.918004025703958 60-61 22.870781548717606 27.126053876448374 27.182061982433787 22.82110259240023 62-63 22.82314476308482 27.0424680605407 27.128049987475105 23.00633718889938 64-65 22.650430121212576 26.867296641582993 27.15520655314719 23.327066684057243 66-67 22.718158545424735 27.00903029024649 27.098488300759882 23.174322863568896 68-69 22.75192273723214 27.000459496885142 27.086549143182086 23.161068622700633 70-71 22.695638174666357 26.777692917112955 27.11552437921086 23.41114452900983 72-73 22.721992272543435 26.970525327451213 26.982629955033126 23.32485244497223 74-75 22.72220072202387 27.121103025250164 26.86985693882856 23.28683931389741 76-77 22.645669321496076 27.000802385324967 26.973054024086878 23.38047426909208 78-79 22.68743041497489 26.939012933293014 26.916295610651638 23.45726104108046 80-81 22.763431048358793 27.039097228165083 26.802250391106895 23.39522133236923 82-83 22.8344535270164 27.014679263178582 26.89225275927813 23.258614450526885 84-85 22.818559314650187 26.994664037335298 26.754920877197257 23.431855770817258 86-87 22.6288996278827 27.136646220182687 26.752037314312783 23.482416837621834 88-89 22.712587399546635 26.89632672458922 26.70722898955176 23.683856886312388 90-91 22.726349653236955 26.907832536486413 26.780788309986413 23.585029500290215 92-93 22.79494013030564 26.757303926798777 26.809045293390376 23.638710649505207 94-95 22.61166578482097 26.79458151125636 26.846745683103705 23.747007020818963 96-97 22.589934086108947 26.580704998676392 26.811736900542588 24.01762401467208 98-99 22.425900440176953 26.698927673641887 26.74880818291371 24.126363703267447 100-101 22.74409092328348 26.71495925313017 26.48434209854947 24.056607725036876 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 34046.0 1 37245.5 2 27725.5 3 15702.0 4 20419.5 5 26440.5 6 28307.0 7 27296.5 8 25342.0 9 22947.5 10 20538.5 11 17965.0 12 15217.5 13 12864.5 14 10817.5 15 9090.5 16 7960.0 17 7216.5 18 6735.0 19 6636.0 20 6994.0 21 7639.0 22 8672.0 23 10163.5 24 11757.5 25 13879.5 26 16561.5 27 19804.0 28 23808.0 29 28635.5 30 34351.0 31 41033.5 32 48425.0 33 54828.0 34 60076.5 35 65435.5 36 71009.0 37 75985.0 38 79271.0 39 81441.5 40 83548.0 41 85459.0 42 87767.5 43 90188.0 44 92061.5 45 94561.5 46 96620.0 47 97408.0 48 97215.0 49 96121.5 50 93945.5 51 90823.0 52 87784.5 53 84145.5 54 79078.0 55 72411.0 56 65291.5 57 57990.5 58 50547.5 59 43015.0 60 35830.5 61 29580.0 62 24006.0 63 18938.0 64 14470.0 65 11090.0 66 8514.5 67 6627.0 68 5126.5 69 3918.0 70 3033.0 71 2304.5 72 1751.5 73 1362.0 74 1032.5 75 800.5 76 575.0 77 387.0 78 265.5 79 160.0 80 105.0 81 67.0 82 40.5 83 25.0 84 18.5 85 13.5 86 10.0 87 6.5 88 3.5 89 1.0 90 0.5 91 0.5 92 0.5 93 1.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.317913279676766 2 21.234023891993736 3 16.979829626739054 4 22.216975338778376 5 22.071226896371293 6 15.961087479344648 7 14.818949066635003 8 11.903878153757802 9 2.998185629217187 10-11 6.435504783263833 12-13 3.5186137159354693 14-15 7.3143842213362245 16-17 11.354633790027663 18-19 14.413650001650048 20-21 14.832353568574405 22-23 12.764096756008467 24-25 15.280026509613986 26-27 12.821559202139548 28-29 12.85944222982869 30-31 13.704078949114265 32-33 12.192704344657642 34-35 19.04709981352773 36-37 23.754036235022426 38-39 22.893783611193236 40-41 18.000613068845045 42-43 17.904757271346924 44-45 23.237809813252152 46-47 12.66885334692382 48-49 21.395983616568646 50-51 21.050885734786146 52-53 11.113386776696988 54-55 12.702177483090423 56-57 12.224480499001295 58-59 13.24113031931633 60-61 12.650090446366512 62-63 12.602528279586611 64-65 13.660956598332058 66-67 12.015792136320384 68-69 13.038923067344694 70-71 11.665931233544189 72-73 11.043693573085665 74-75 11.122742710791748 76-77 11.170849222828071 78-79 11.566418116354479 80-81 11.544150993208953 82-83 10.073534239826952 84-85 9.994570156067187 86-87 10.12266139921907 88-89 11.51764818353689 90-91 9.030653782468068 92-93 11.035987685549436 94-95 11.137474054293676 96-97 10.679917844692174 98-99 10.547624936592284 100-101 10.652054171879056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2939311.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.9736501337998 #Duplication Level Percentage of deduplicated Percentage of total 1 98.82205539895575 95.83135425761317 2 0.804851153728826 1.5609870838296858 3 0.16619908016151733 0.4835079435642698 4 0.07295714878225404 0.28299684083079524 5 0.0317645012039303 0.15401598132122996 6 0.02283701379802912 0.13287531516905007 7 0.017478675995086367 0.11864797075246851 8 0.009791632205722314 0.07596242526052496 9 0.009890463081718459 0.08632028758930461 >10 0.0390197971899191 0.6420246289400642 >50 0.0018483553395888862 0.1195359866934259 >100 0.001166373104710849 0.20371547220760797 >500 3.5101613231339015E-5 0.02362328503075712 >1k 7.020322646059682E-5 0.10007558787949135 >5k 3.510161323029841E-5 0.1843569333181378 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5416 0.18426086929896157 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.80431570527923E-5 2 0.0 0.0 0.0 0.0 6.80431570527923E-5 3 0.0 0.0 0.0 0.0 6.80431570527923E-5 4 0.0 0.0 0.0 0.0 1.0206473557918845E-4 5 0.0 0.0 0.0 0.0 1.0206473557918845E-4 6 3.402157852639615E-5 0.0 0.0 0.0 1.7010789263198077E-4 7 3.402157852639615E-5 0.0 0.0 0.0 1.7010789263198077E-4 8 3.402157852639615E-5 0.0 0.0 0.0 1.7010789263198077E-4 9 3.402157852639615E-5 0.0 0.0 0.0 1.7010789263198077E-4 10-11 3.402157852639615E-5 0.0 0.0 6.80431570527923E-5 1.7010789263198077E-4 12-13 3.402157852639615E-5 0.0 0.0 6.80431570527923E-5 2.721726282111692E-4 14-15 3.402157852639615E-5 0.0 0.0 6.80431570527923E-5 3.572265745271596E-4 16-17 6.80431570527923E-5 0.0 0.0 6.80431570527923E-5 3.742373637903577E-4 18-19 6.80431570527923E-5 0.0 0.0 6.80431570527923E-5 3.742373637903577E-4 20-21 6.80431570527923E-5 0.0 0.0 8.505394631599039E-5 4.5929131010634806E-4 22-23 6.80431570527923E-5 0.0 0.0 1.360863141055846E-4 5.273344671591403E-4 24-25 8.505394631599039E-5 0.0 0.0 2.041294711583769E-4 5.443452564223384E-4 26-27 1.0206473557918845E-4 0.0 0.0 3.0619420673756536E-4 5.443452564223384E-4 28-29 1.0206473557918845E-4 0.0 0.0 7.144531490543192E-4 5.443452564223384E-4 30-31 1.0206473557918845E-4 0.0 0.0 0.002466564443163721 5.783668349487346E-4 32-33 1.360863141055846E-4 0.0 0.0 0.007110509912016796 6.123884134751307E-4 34-35 1.7010789263198077E-4 0.0 0.0 0.014544224820034354 6.464099920015268E-4 36-37 1.7010789263198077E-4 0.0 0.0 0.026928079403642554 6.464099920015268E-4 38-39 1.7010789263198077E-4 0.0 0.0 0.04572500153947643 6.464099920015268E-4 40-41 2.041294711583769E-4 0.0 0.0 0.06525338761362783 6.464099920015268E-4 42-43 2.041294711583769E-4 0.0 1.7010789263198076E-5 0.08513900026230636 6.63420781264725E-4 44-45 2.041294711583769E-4 0.0 3.402157852639615E-5 0.1115057236202634 6.974423597911211E-4 46-47 2.041294711583769E-4 0.0 3.402157852639615E-5 0.14440459005528847 7.314639383175173E-4 48-49 2.041294711583769E-4 0.0 3.402157852639615E-5 0.17915763252000214 8.335286738967056E-4 50-51 2.3815104968477306E-4 0.0 3.402157852639615E-5 0.22239905882705163 8.845610416863E-4 52-53 2.3815104968477306E-4 0.0 3.402157852639615E-5 0.27919808417687 9.355934094758941E-4 54-55 2.3815104968477306E-4 0.0 3.402157852639615E-5 0.3436519646951275 0.0010206473557918846 56-57 2.3815104968477306E-4 0.0 3.402157852639615E-5 0.4176488979900391 0.0010716797235814789 58-59 2.3815104968477306E-4 0.0 3.402157852639615E-5 0.5011378516938153 0.001088690512844677 60-61 2.891834174743673E-4 0.0 3.402157852639615E-5 0.5933363295003489 0.0011227120913710731 62-63 3.4021578526396154E-4 0.0 3.402157852639615E-5 0.6867595841338328 0.0011907552484238654 64-65 3.4021578526396154E-4 0.0 3.402157852639615E-5 0.7857283560671191 0.0011907552484238654 66-67 3.4021578526396154E-4 0.0 3.402157852639615E-5 0.9018269927884459 0.0012077660376870634 68-69 3.4021578526396154E-4 0.0 3.402157852639615E-5 1.0280980814891651 0.0012247768269502614 70-71 3.4021578526396154E-4 0.0 3.402157852639615E-5 1.1658004205747536 0.0012587984054766577 72-73 3.4021578526396154E-4 0.0 3.402157852639615E-5 1.3131478771725755 0.0013948847195822422 74-75 3.4021578526396154E-4 0.0 3.402157852639615E-5 1.4650031929251446 0.0014289062981086384 76-77 3.4021578526396154E-4 0.0 3.402157852639615E-5 1.631385722708485 0.0014459170873718365 78-79 3.4021578526396154E-4 0.0 3.402157852639615E-5 1.8249004613666262 0.0014629278766350345 80-81 3.4021578526396154E-4 0.0 3.402157852639615E-5 2.039797762128608 0.0014969494551614307 82-83 3.4021578526396154E-4 0.0 3.402157852639615E-5 2.264136050931664 0.0015990141907406192 84-85 3.4021578526396154E-4 1.7010789263198076E-5 3.402157852639615E-5 2.4952106122829463 0.0015990141907406192 86-87 3.4021578526396154E-4 3.402157852639615E-5 3.402157852639615E-5 2.7357431724645673 0.0016500465585302135 88-89 3.4021578526396154E-4 3.402157852639615E-5 3.402157852639615E-5 3.0012305604952996 0.0016840681370566095 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2280 0.0 52.55528 1 GTATCAA 4185 0.0 42.34688 1 ATCAACG 5020 0.0 34.89004 3 TCAACGC 5160 0.0 34.228245 4 TATCAAC 5230 0.0 33.39667 2 CAACGCA 5390 0.0 32.452595 5 AACGCAG 5960 0.0 30.221855 6 GTACATG 6470 0.0 28.247274 1 TACATGG 6635 0.0 26.779915 2 ACATGGG 6725 0.0 25.969475 3 ACGCAGA 6935 0.0 25.723473 7 GTGGTAT 1155 0.0 24.846607 1 CGCAGAG 7355 0.0 24.24516 8 CATGGGG 5075 0.0 22.903568 4 TATCACG 235 6.1419996E-8 21.419413 2 GCAGAGT 8295 0.0 20.499866 9 AGAGTAC 7280 0.0 19.103718 10-11 GTATAGC 965 0.0 18.782328 1 TGGTATC 1380 0.0 18.237543 2 CAGAGTA 7685 0.0 18.125721 10-11 >>END_MODULE