FastQCFastQC Report
Sat 18 Jun 2016
SRR1930937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939311
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.13122122837630995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33530.1140743527990063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31950.054.0881271
GTATCAA57050.038.1137121
TCAACGC69950.030.9433464
ATCAACG70000.030.8544833
CAACGCA71400.030.3066945
TATCAAC73200.029.5710052
AACGCAG75350.029.15886
ACGCAGA88400.024.5858467
GTACATG82600.024.5425281
CGCAGAG91150.023.7920278
TACATGG84850.023.1052442
ACATGGG83450.022.6961143
GCAGAGT107300.019.9456759
TATCACG2953.0449883E-919.3095232
CATGGGG63000.019.2881054
AGAGTAC95450.018.69320910-11
GTGGTAT17150.018.5473611
GAGTACA66100.018.386961
CAGAGTA100950.018.21534210-11
GAGTACT62950.017.5643512-13
Produced by FastQC (version 0.11.5)