##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930937_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2939311 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.187252046483003 31.0 31.0 33.0 30.0 34.0 2 31.311917997108846 31.0 31.0 34.0 30.0 34.0 3 31.39222695386776 31.0 31.0 34.0 28.0 34.0 4 35.26141534529691 37.0 35.0 37.0 33.0 37.0 5 35.04496768120148 35.0 35.0 37.0 32.0 37.0 6 35.08327325689592 36.0 35.0 37.0 32.0 37.0 7 34.962878375238276 36.0 35.0 37.0 32.0 37.0 8 34.93927318340931 36.0 35.0 37.0 32.0 37.0 9 36.43242923256505 38.0 35.0 39.0 32.0 39.0 10-11 36.35777908496243 38.0 35.0 39.0 32.0 39.0 12-13 36.24788717491956 38.0 35.0 39.0 32.0 39.0 14-15 37.25255136322764 39.0 36.0 40.0 32.0 41.0 16-17 37.2143541462608 39.0 36.0 40.0 31.5 41.0 18-19 37.18821162510534 39.0 36.0 40.0 31.0 41.0 20-21 37.142415858682526 39.0 36.0 40.0 31.0 41.0 22-23 37.01070625054647 39.0 36.0 40.0 31.0 41.0 24-25 36.88444451777985 39.0 36.0 40.0 31.0 41.0 26-27 36.59827932464445 38.0 35.0 40.0 30.0 41.0 28-29 36.400225597087214 38.0 35.0 40.0 30.0 41.0 30-31 36.16270853951828 38.0 35.0 40.0 29.5 41.0 32-33 35.92668196730458 38.0 34.0 40.0 29.0 41.0 34-35 36.136158099636276 38.0 35.0 40.0 30.0 41.0 36-37 36.19467334351486 38.0 35.0 40.0 30.0 41.0 38-39 36.161089282488305 38.0 35.0 40.0 30.0 41.0 40-41 36.06058256509774 38.0 35.0 40.0 29.5 41.0 42-43 35.92230134885352 38.0 35.0 40.0 29.0 41.0 44-45 35.735490902459794 38.0 34.0 40.0 28.5 41.0 46-47 35.51529746256861 38.0 34.0 40.0 27.5 41.0 48-49 35.31379173554619 38.0 34.0 40.0 27.0 41.0 50-51 35.039906120856216 38.0 33.5 40.0 26.5 41.0 52-53 34.8193995123347 38.0 33.0 40.0 26.0 41.0 54-55 34.63371739159279 38.0 33.0 40.0 26.0 41.0 56-57 34.364335553468145 37.0 33.0 40.0 25.5 41.0 58-59 34.10593026733136 37.0 32.5 40.0 25.0 41.0 60-61 33.78304320298193 36.5 32.0 39.5 24.5 41.0 62-63 33.46234168483703 36.0 32.0 39.0 23.0 41.0 64-65 33.142451581339984 36.0 31.0 39.0 23.0 40.0 66-67 32.79746954303236 35.0 31.0 39.0 22.0 40.0 68-69 32.411501709073995 35.0 31.0 38.0 22.0 40.0 70-71 31.991702987536875 35.0 30.5 37.5 21.5 40.0 72-73 31.559389428338818 34.5 30.0 37.0 20.0 39.0 74-75 31.026763414963575 34.0 30.0 36.5 18.5 39.0 76-77 29.682958523272973 32.5 28.5 35.0 18.0 37.5 78-79 30.438579993746835 34.0 29.0 36.0 18.0 38.0 80-81 30.439493132914482 34.0 30.0 35.0 18.0 37.0 82-83 30.161491247438597 34.0 29.0 35.0 18.0 37.0 84-85 29.834450658674772 34.0 29.0 35.0 16.0 36.0 86-87 29.489790124284227 34.0 29.0 35.0 12.0 36.0 88-89 29.136219508585516 34.0 29.0 35.0 7.0 36.0 90-91 28.78405823677726 33.0 29.0 35.0 4.5 35.0 92-93 28.407720891052357 33.0 28.5 35.0 2.0 35.0 94-95 27.96712954158304 33.0 27.0 35.0 2.0 35.0 96-97 27.474488409018306 33.0 27.0 35.0 2.0 35.0 98-99 26.945671961898555 33.0 26.0 35.0 2.0 35.0 100-101 25.68749308936686 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5028071688746039 1101 2 0.67213599390886 1101 3 0.6544119951161598 1101 4 0.46501300279907554 1101 5 0.5090819409920542 1101 6 0.5227703323011426 1101 7 0.5817996105339063 1101 8 0.5174923953492652 1101 9 0.789412716017857 1101 10-11 0.707811098174858 1101 12-13 0.7584710727352117 1101 14-15 0.8486571390037057 1101 16-17 0.8469525235586204 1101 18-19 0.9411804890337905 1101 20-21 0.8891515005055481 1101 22-23 0.8521682722963888 1101 24-25 0.8442799337463001 1101 26-27 0.8864595138795508 1101 28-29 0.9238792277724528 1101 30-31 0.9889069626950899 1101 32-33 0.9346576674595326 1101 34-35 0.9209970364788447 1101 36-37 0.9741736297828396 1101 38-39 1.0715094233986378 1101 40-41 1.108517552935858 1101 42-43 1.0011067262718925 1101 44-45 1.1680223018348102 1101 46-47 1.1874168838943078 1101 48-49 1.1948301541257251 1101 50-51 1.0612086064064314 1101 52-53 1.1910555919329155 1101 54-55 1.0214947347732206 1101 56-57 1.3092537896950347 1101 58-59 1.103717880499012 1101 60-61 1.1659169570746641 1101 62-63 1.1663230363236963 1101 64-65 1.1514985925309702 1101 66-67 1.251135654571101 1101 68-69 1.1312816530422296 1101 70-71 1.0917908420923759 1101 72-73 1.098589987339981 1101 74-75 1.0764395582884951 1101 76-77 1.1745812802572786 1101 78-79 1.2892500361600376 1101 80-81 1.164909008376771 1101 82-83 1.3276269500330358 1101 84-85 1.3783429801526097 1101 86-87 1.2479009808290193 1101 88-89 1.2221113357006175 1101 90-91 1.1284490320385387 1101 92-93 1.2710477339250374 1101 94-95 1.4196306108168102 1101 96-97 1.3399291384881202 1101 98-99 1.3447872833131953 1101 100-101 1.6508531897523753 1102 1 0.48804651134744503 1102 2 0.41989606345391906 1102 3 0.470321046383134 1102 4 0.29409026477182465 1102 5 0.5082686689138853 1102 6 0.4243174848079434 1102 7 0.47807653040798215 1102 8 0.6077174321333132 1102 9 0.6924024221112148 1102 10-11 0.6839827439339388 1102 12-13 0.6434752786358473 1102 14-15 0.6710344997236888 1102 16-17 0.5835669282759923 1102 18-19 0.7101651367931225 1102 20-21 0.7115206463560497 1102 22-23 0.7377162913031228 1102 24-25 0.6855984202972607 1102 26-27 0.7357058558713234 1102 28-29 0.7662771256661216 1102 30-31 0.780163584526953 1102 32-33 0.695006405548682 1102 34-35 0.7395722067545165 1102 36-37 0.7550438432850513 1102 38-39 0.783357403300343 1102 40-41 0.8112061221700415 1102 42-43 0.7654898092673932 1102 44-45 0.880109469477226 1102 46-47 0.889579026287258 1102 48-49 0.7964115727661607 1102 50-51 0.7610453780752593 1102 52-53 0.7462626094570268 1102 54-55 0.6959783936535828 1102 56-57 0.6635923379700444 1102 58-59 0.7603770168686594 1102 60-61 0.8497479558283629 1102 62-63 0.6643428026500473 1102 64-65 0.6368723550255879 1102 66-67 0.7945145541724585 1102 68-69 0.7017996680821952 1102 70-71 0.8029892846431501 1102 72-73 0.6817260418957183 1102 74-75 0.8516514147083285 1102 76-77 0.9661155759306865 1102 78-79 1.0697591892688898 1102 80-81 0.9259336726783793 1102 82-83 1.0352627837438177 1102 84-85 1.1304271656848712 1102 86-87 1.028169201944042 1102 88-89 0.9700358714338932 1102 90-91 0.9196492751086467 1102 92-93 1.0604846496354128 1102 94-95 0.9498905889292502 1102 96-97 0.8576528319159458 1102 98-99 0.9553069863400374 1102 100-101 1.3064284697471962 1103 1 0.484343491069108 1103 2 0.5964967611019212 1103 3 0.4980444364315382 1103 4 0.2950156596577713 1103 5 0.3474514399451465 1103 6 0.45048964704574246 1103 7 0.5595033009183581 1103 8 0.5380084882194467 1103 9 0.5888922940243688 1103 10-11 0.5906529016112501 1103 12-13 0.5947490819257055 1103 14-15 0.6854155241413693 1103 16-17 0.6065502462322527 1103 18-19 0.7474078479230784 1103 20-21 0.7583619189679638 1103 22-23 0.7811646894556574 1103 24-25 0.7803873388320355 1103 26-27 0.8748823652485598 1103 28-29 0.892250688187076 1103 30-31 0.7230956455808482 1103 32-33 0.7173886047191473 1103 34-35 0.5860148391981994 1103 36-37 0.7628018109627277 1103 38-39 0.7279168294446876 1103 40-41 0.7304284579886442 1103 42-43 0.6912148450779583 1103 44-45 0.6986539951015232 1103 46-47 0.6581035201599761 1103 48-49 0.6355264630348358 1103 50-51 0.6421883238787061 1103 52-53 0.58719581755728 1103 54-55 0.755556480081033 1103 56-57 0.8508454390126445 1103 58-59 0.7859136489299914 1103 60-61 0.6554412954787665 1103 62-63 0.5656799705538091 1103 64-65 0.6131042800408864 1103 66-67 0.39218405520729505 1103 68-69 0.5055977768410358 1103 70-71 0.5658399996076717 1103 72-73 0.5337705273456841 1103 74-75 0.6682973846690139 1103 76-77 0.8209306493388837 1103 78-79 0.9696908097904888 1103 80-81 0.9711727376416555 1103 82-83 0.7982840595142662 1103 84-85 0.7771307373212437 1103 86-87 0.9151252346043854 1103 88-89 0.7550140526173443 1103 90-91 0.9329123449260912 1103 92-93 0.9688595287660995 1103 94-95 0.9338333252720545 1103 96-97 0.8508510132656362 1103 98-99 1.044022876256836 1103 100-101 1.3133029900795634 1104 1 0.33646993880168097 1104 2 0.46170103713748745 1104 3 0.39462669119672356 1104 4 0.20222544527190678 1104 5 0.33276114547857105 1104 6 0.3890844332136396 1104 7 0.4098229056279621 1104 8 0.4537357465258651 1104 9 0.5637439500098864 1104 10-11 0.47702717952036267 1104 12-13 0.49469239665439346 1104 14-15 0.6489116137832482 1104 16-17 0.5959059587574913 1104 18-19 0.6686817089592694 1104 20-21 0.6658698129345879 1104 22-23 0.5777625518208893 1104 24-25 0.5786749074264819 1104 26-27 0.6435079931831709 1104 28-29 0.530069945795141 1104 30-31 0.5174993474865346 1104 32-33 0.6470864205169136 1104 34-35 0.6530276179606318 1104 36-37 0.7164035120315475 1104 38-39 0.5716775716387303 1104 40-41 0.5843207349076849 1104 42-43 0.5963405852021637 1104 44-45 0.66701541094384 1104 46-47 0.6714325382631969 1104 48-49 0.7173393320596162 1104 50-51 0.6092990570880019 1104 52-53 0.7232977604002642 1104 54-55 0.5318756334788404 1104 56-57 0.6842240950291227 1104 58-59 0.7322318033137307 1104 60-61 0.709735156904344 1104 62-63 0.7910894625918559 1104 64-65 0.8711248788812043 1104 66-67 0.8180967886151223 1104 68-69 0.692252863881329 1104 70-71 0.6705441749438954 1104 72-73 0.840309537249329 1104 74-75 0.9504152881182897 1104 76-77 0.9226928399437462 1104 78-79 0.9753409844264489 1104 80-81 0.8473490926701928 1104 82-83 0.9787439826125244 1104 84-85 1.1246468787395045 1104 86-87 1.0904477358164506 1104 88-89 1.1161509370148188 1104 90-91 0.9834581481254219 1104 92-93 0.9739524259037147 1104 94-95 0.946557987737755 1104 96-97 0.8929695103782294 1104 98-99 1.0504674745430655 1104 100-101 1.2643929527330329 1105 1 0.11570109194773437 1105 2 0.22570762003035227 1105 3 0.1890386238857431 1105 4 0.005232469488170466 1105 5 0.07331392089341193 1105 6 0.15640948233843233 1105 7 0.14753860205087932 1105 8 0.20959663617296798 1105 9 0.21002899150701637 1105 10-11 0.14826174523609126 1105 12-13 0.23897110930690246 1105 14-15 0.32423610760456967 1105 16-17 0.19708563701068016 1105 18-19 0.32269550023782756 1105 20-21 0.31086396019746587 1105 22-23 0.26397726730083804 1105 24-25 0.32270671501071746 1105 26-27 0.3171080336818122 1105 28-29 0.307785039707575 1105 30-31 0.30583834721354464 1105 32-33 0.32229336267994313 1105 34-35 0.3501504130129618 1105 36-37 0.503941700791863 1105 38-39 0.34361384466418343 1105 40-41 0.2868192278550552 1105 42-43 0.25562075288642205 1105 44-45 0.26064041525302883 1105 46-47 0.2735785625791536 1105 48-49 0.3297993109220556 1105 50-51 0.3292582416614991 1105 52-53 0.4224662959364309 1105 54-55 0.47827595945565093 1105 56-57 0.4897309165968835 1105 58-59 0.5004690036152368 1105 60-61 0.3476451474287785 1105 62-63 0.28245648123023415 1105 64-65 0.20832934205523657 1105 66-67 0.220155293625119 1105 68-69 0.1411511237751526 1105 70-71 0.23763092185626178 1105 72-73 0.14032699141892735 1105 74-75 0.15241577820156138 1105 76-77 0.26382604414106936 1105 78-79 0.36197180482521674 1105 80-81 0.36496010134417034 1105 82-83 0.41183829941688543 1105 84-85 0.38888840966273364 1105 86-87 0.4790865621508793 1105 88-89 0.4363447279890238 1105 90-91 0.3521231308684669 1105 92-93 0.4096230961593541 1105 94-95 0.2463591282217692 1105 96-97 0.13766097376748831 1105 98-99 0.38575896693978606 1105 100-101 0.6777556724280771 1106 1 0.0471787531543022 1106 2 0.15913627961686672 1106 3 0.1075205686394547 1106 4 0.13613492202286892 1106 5 0.03686880495300926 1106 6 0.055792438077972406 1106 7 0.1306099108729768 1106 8 0.15222943196192773 1106 9 0.11441933595131815 1106 10-11 0.24949880886850906 1106 12-13 0.16268250263443207 1106 14-15 0.19314487350991527 1106 16-17 0.03739744709918824 1106 18-19 0.1628283435672273 1106 20-21 0.1360901146787512 1106 22-23 0.2731686749334372 1106 24-25 0.14606989760711997 1106 26-27 0.20487655283078965 1106 28-29 0.13827545463441737 1106 30-31 0.11694084992641507 1106 32-33 0.3266891666072098 1106 34-35 0.31351268160602785 1106 36-37 0.3690131862803696 1106 38-39 0.31600143605685105 1106 40-41 0.17421033724038892 1106 42-43 0.2873960172572865 1106 44-45 0.1657555213970383 1106 46-47 0.19671717026377422 1106 48-49 0.2790288797271856 1106 50-51 0.22093949250879774 1106 52-53 0.2137645241808528 1106 54-55 0.2743648840049744 1106 56-57 0.34260214456955396 1106 58-59 0.26721903474656017 1106 60-61 0.11791715849987128 1106 62-63 0.15279452750825584 1106 64-65 0.17505317993681047 1106 66-67 0.0652984394282825 1106 68-69 0.18549730774991957 1106 70-71 0.11044337144039318 1106 72-73 0.17149220457493541 1106 74-75 0.28293928740999164 1106 76-77 0.17744950912155488 1106 78-79 0.27543485915443355 1106 80-81 0.3712823666492788 1106 82-83 0.40629416379105976 1106 84-85 0.47791494090968456 1106 86-87 0.41523177458304517 1106 88-89 0.5508265586456815 1106 90-91 0.46517016032698066 1106 92-93 0.6482572500713815 1106 94-95 0.5736687808943728 1106 96-97 0.6490053499786193 1106 98-99 0.599827287768445 1106 100-101 0.9942299383269813 1107 1 0.17376873724568043 1107 2 0.1595321383563224 1107 3 0.2294793868846874 1107 4 -0.01191383765654308 1107 5 0.1210206043337223 1107 6 0.19921856334959642 1107 7 0.14789721458716087 1107 8 0.0441364091729497 1107 9 0.048779051503125004 1107 10-11 0.21030974717618278 1107 12-13 0.1456403021255639 1107 14-15 0.1702934158885725 1107 16-17 0.1936602254373696 1107 18-19 0.2377916834747964 1107 20-21 0.27190574527750755 1107 22-23 0.23460903265163324 1107 24-25 0.13138152093909383 1107 26-27 0.15376977435209227 1107 28-29 0.19681507312856894 1107 30-31 0.21872773459642048 1107 32-33 0.2322154308910953 1107 34-35 0.11734630852120631 1107 36-37 0.22743582369926685 1107 38-39 0.29380403864739435 1107 40-41 0.15450923869224198 1107 42-43 0.2953991588339946 1107 44-45 0.2753096282809011 1107 46-47 0.2884329930708205 1107 48-49 0.24132654662972186 1107 50-51 0.22754177190838476 1107 52-53 0.1693420774647052 1107 54-55 0.21932385782350394 1107 56-57 0.34280890648722107 1107 58-59 0.32866656890995216 1107 60-61 0.18001990712856752 1107 62-63 0.03848956371667356 1107 64-65 0.03250790058238806 1107 66-67 -0.032586617004675134 1107 68-69 0.18380853462093683 1107 70-71 -0.07606451010309456 1107 72-73 -0.01175109699133614 1107 74-75 0.17176787073956135 1107 76-77 0.20961719705157833 1107 78-79 0.38009598540466527 1107 80-81 0.37558893635865687 1107 82-83 0.3614137795752228 1107 84-85 0.4074588602524649 1107 86-87 0.3132548897624794 1107 88-89 0.3852558806974393 1107 90-91 0.3986249913794069 1107 92-93 0.3675289161609214 1107 94-95 0.451430984615083 1107 96-97 0.5095128200056713 1107 98-99 0.5128799367800561 1107 100-101 0.8737599062588117 1108 1 0.24492458333095612 1108 2 0.30001497894464535 1108 3 0.23010435593337064 1108 4 0.06508017016794554 1108 5 0.2078754550245563 1108 6 0.17018028275731467 1108 7 0.21534781628081845 1108 8 0.14605737188279733 1108 9 0.19522732565753387 1108 10-11 0.2248484487064104 1108 12-13 0.2991343347111055 1108 14-15 0.3194899196274932 1108 16-17 0.2719898446123281 1108 18-19 0.30288750876078296 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2316 70-71 -0.6490245906744185 2316 72-73 -0.6223010573160614 2316 74-75 -0.502003721764865 2316 76-77 -0.9801178942015518 2316 78-79 -0.9228302185872757 2316 80-81 -0.8393161481801918 2316 82-83 -1.1037955837892426 2316 84-85 -1.084000884605011 2316 86-87 -0.9554172093646649 2316 88-89 -1.1056914427475952 2316 90-91 -0.9279290585503972 2316 92-93 -0.8979783169465669 2316 94-95 -0.6127486528139592 2316 96-97 -0.6501938881674398 2316 98-99 -0.7176797897657536 2316 100-101 -0.9192392391968447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 22.0 4 65.0 5 109.0 6 237.0 7 619.0 8 1448.0 9 2488.0 10 3824.0 11 5958.0 12 7953.0 13 10063.0 14 11863.0 15 14619.0 16 17055.0 17 19153.0 18 18319.0 19 17015.0 20 17584.0 21 18690.0 22 21109.0 23 24640.0 24 30075.0 25 38319.0 26 48154.0 27 61290.0 28 77009.0 29 95093.0 30 114311.0 31 136624.0 32 163303.0 33 195126.0 34 237193.0 35 293095.0 36 372192.0 37 460040.0 38 354838.0 39 49806.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.796450751557714 20.798682983282422 14.368732694674597 24.03613357048527 2 15.953321425533726 21.565875648549802 39.05792293952539 23.422879986391088 3 19.0634071259528 25.772005438162676 30.22035037771823 24.944237058166294 4 13.503181245033295 17.577489139183513 37.47011467456689 31.449214941216308 5 13.343782661237643 37.94038235422201 35.039894340896225 13.675940643644125 6 31.38954380449833 37.60607081253763 17.4029959412243 13.601389441739729 7 27.004798063219575 32.36057701958044 22.54242575896188 18.092199158238103 8 25.34318416799039 36.29891494979606 20.350891756605545 18.007009125608008 9 25.870654721463637 15.868616828909904 20.382974105155935 37.877754344470524 10-11 24.78403952490907 26.366723357957017 28.61531154750212 20.233925569631793 12-13 25.163175996007226 23.72666587509794 28.71924746990026 22.390910658994574 14-15 22.392861316577925 25.463123226730634 25.946382985527723 26.197632471163722 16-17 22.386828069571408 28.384134921415257 26.692190788929786 22.53684622008355 18-19 22.367622888493255 27.457472176302545 28.31502008463888 21.85988485056532 20-21 22.972832748899314 26.735143031819362 28.242928359741448 22.04909585953987 22-23 22.733175473155264 26.549360223998146 28.135769959616376 22.581694343230215 24-25 22.613647884630954 26.714559054576974 28.085974950481372 22.585818110310694 26-27 22.594606327649245 26.862586794835025 27.97514611675936 22.567660760756368 28-29 22.61144622377361 27.016663774831507 27.919681830089377 22.45220817130551 30-31 22.85816551643419 26.787476234350738 27.90811931206526 22.446238937149808 32-33 22.704938952182612 26.863795990036344 27.82761307798059 22.603651979800453 34-35 22.856971268970124 26.917155747643573 27.852887300329147 22.372985683057152 36-37 22.82238764001461 26.775772324725196 27.867174793653504 22.534665241606692 38-39 22.77458851931595 26.789549937765656 27.87043893370975 22.56542260920864 40-41 22.929364101356743 26.744140155863317 27.80888746146115 22.517608281318786 42-43 22.815893136173294 26.842306275715 27.626551618838285 22.715248969273418 44-45 22.764019069990894 26.868041161347744 27.691533506540168 22.67640626212119 46-47 22.888662513656516 26.88031226172334 27.425892449762223 22.805132774857924 48-49 22.839506802929403 26.933173144318463 27.384597901074777 22.842722151677357 50-51 22.88279122399801 27.021210602915563 27.280949313373622 22.815048859712807 52-53 22.99383131017689 26.983499443990794 27.25784497163111 22.764824274201207 54-55 22.79194964752656 26.8811145217968 27.438450280607444 22.888485550069195 56-57 22.83102319461613 27.01086185407899 27.269061834276343 22.889053117028542 58-59 22.84247817603587 27.031328371119344 27.33674817319792 22.78944527964687 60-61 22.868726918161517 27.117648850892706 27.349269286796613 22.664354944149164 62-63 22.900583082684683 26.98502237746878 27.359867327192905 22.754527212653635 64-65 22.66363009441753 26.891253820547096 27.310402955975377 23.134713129059996 66-67 22.70321401295686 27.03006267278438 27.23625877278498 23.030464541473787 68-69 22.783303049204005 26.968762137703646 27.3261778670267 22.921756946065646 70-71 22.714662622187383 26.77293867424348 27.302431522965747 23.20996718060339 72-73 22.69711655491381 26.99363527883743 27.193122220009297 23.116125946239467 74-75 22.722404055546196 27.01940133907228 27.07623040991271 23.181964195468815 76-77 22.608740745441253 26.909856734091335 27.164656686191606 23.316745834275803 78-79 22.583486725483056 26.943237532705698 27.17225399698546 23.30102174482578 80-81 22.69800694692875 27.01378515757831 27.021797311556604 23.266410583936327 82-83 22.72114111096104 26.949904246267238 27.155445612934457 23.173509029837263 84-85 22.68857702804009 26.92919884755246 26.99052277414134 23.391701350266107 86-87 22.535281227471334 27.02230216537141 27.06491419247572 23.377502414681537 88-89 22.55938986958146 26.826893831401016 27.04558491017948 23.568131388838047 90-91 22.57211726164928 26.832712994486958 27.066813717509614 23.528356026354153 92-93 22.612289105954204 26.724344442864055 27.14586031431898 23.51750613686276 94-95 22.43570922370676 26.67845647021039 27.15821959450379 23.727614711579058 96-97 22.34705684427405 26.584189287897743 27.199503557126143 23.869250310702068 98-99 22.21840424507648 26.625916753960365 27.084051330396818 24.071627670566333 100-101 22.318274588840715 26.55965972978021 27.041966637759668 24.080099043619406 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 196.0 2 169.5 3 227.0 4 212.0 5 201.0 6 278.5 7 532.5 8 822.0 9 960.5 10 903.0 11 806.0 12 873.0 13 973.5 14 1117.5 15 1297.5 16 1487.5 17 1699.5 18 1923.5 19 2162.0 20 2473.5 21 2912.5 22 3554.5 23 4517.0 24 5844.0 25 7826.0 26 10391.0 27 13516.0 28 17638.0 29 23369.5 30 30289.5 31 38151.5 32 47648.5 33 55631.0 34 61440.0 35 67163.0 36 74778.0 37 81913.0 38 85671.5 39 88611.5 40 91140.0 41 93673.0 42 96577.5 43 99996.5 44 103549.0 45 107719.0 46 111274.5 47 113799.5 48 115627.5 49 115632.5 50 113349.5 51 110847.0 52 109351.0 53 107006.5 54 103044.0 55 95408.0 56 86125.5 57 76812.5 58 67225.5 59 57975.0 60 48724.5 61 40057.0 62 32140.5 63 24704.0 64 18776.0 65 14210.5 66 10530.0 67 8304.5 68 6472.0 69 4798.5 70 3609.5 71 2776.0 72 2117.0 73 1597.0 74 1227.5 75 947.0 76 682.0 77 439.0 78 296.5 79 193.0 80 112.5 81 61.5 82 36.0 83 21.0 84 13.5 85 6.0 86 2.0 87 3.0 88 2.5 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01864382503246509 2 0.0020753162901101653 3 0.002823791017690881 4 0.03388549221229057 5 0.0015990141907406192 6 3.402157852639615E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.1032367789594226E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.402157852639615E-5 24-25 4.4228052084314994E-4 26-27 0.0017691220833725998 28-29 3.402157852639615E-5 30-31 0.0066342078126472494 32-33 0.02146761605015597 34-35 0.012945210629293736 36-37 6.63420781264725E-4 38-39 5.953776242119326E-4 40-41 0.0023474889183213348 42-43 0.008284254371177463 44-45 0.008267243581914266 46-47 0.008097135689282284 48-49 0.00962810672297011 50-51 0.18375054562106563 52-53 0.047970425722218574 54-55 0.017282961891409244 56-57 0.010546689343182807 58-59 0.01935827818151941 60-61 0.05207002593464931 62-63 0.09401863225769576 64-65 0.0981862756271793 66-67 0.10303435056719075 68-69 0.10051675375623743 70-71 0.08957881626000107 72-73 0.0689277180944786 74-75 0.23246944607086492 76-77 0.27856868497413173 78-79 0.007518768854333549 80-81 9.01571830949498E-4 82-83 0.0 84-85 5.1032367789594226E-5 86-87 0.0 88-89 1.7010789263198074E-4 90-91 0.006515132287804862 92-93 0.017453069784041226 94-95 0.0016330357692670152 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2939311.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.63351970094466 #Duplication Level Percentage of deduplicated Percentage of total 1 78.82737899508206 48.58408816267221 2 12.283621407476929 15.141656440333499 3 3.7214627292556113 6.881005393197209 4 1.6639905784679936 4.102303844007736 5 0.9106175727893285 2.806228305626875 6 0.5646032603242548 2.0879091701047523 7 0.38498601167818275 1.6609630054748772 8 0.2758511768465407 1.3601343154160028 9 0.20931765819799739 1.1610885609271684 >10 1.0922064921211037 11.808388249266383 >50 0.04862840907219958 2.0223977017387953 >100 0.01639394919616353 1.7633811460373103 >500 7.755666402814217E-4 0.30483317946978156 >1k 1.6619285139479167E-4 0.31562252572746413 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3857 0.13122122837630995 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3353 0.1140743527990063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.80431570527923E-5 0.0 0.0 0.0 0.0 7 6.80431570527923E-5 0.0 0.0 0.0 3.402157852639615E-5 8 6.80431570527923E-5 0.0 0.0 0.0 3.402157852639615E-5 9 6.80431570527923E-5 0.0 0.0 0.0 6.80431570527923E-5 10-11 6.80431570527923E-5 0.0 0.0 3.402157852639615E-5 1.360863141055846E-4 12-13 6.80431570527923E-5 0.0 0.0 3.402157852639615E-5 4.933128886327442E-4 14-15 6.80431570527923E-5 0.0 0.0 3.402157852639615E-5 6.293992027383288E-4 16-17 1.0206473557918845E-4 0.0 0.0 3.402157852639615E-5 7.144531490543192E-4 18-19 1.0206473557918845E-4 0.0 0.0 3.402157852639615E-5 8.165178846335076E-4 20-21 1.0206473557918845E-4 0.0 0.0 5.1032367789594226E-5 9.866257772654884E-4 22-23 1.0206473557918845E-4 0.0 0.0 1.360863141055846E-4 0.0010376581450550826 24-25 1.0206473557918845E-4 0.0 0.0 2.041294711583769E-4 0.001088690512844677 26-27 1.0206473557918845E-4 0.0 0.0 4.082589423167538E-4 0.0011567336698974692 28-29 1.0206473557918845E-4 0.0 0.0 0.0012077660376870634 0.0012077660376870634 30-31 1.0206473557918845E-4 0.0 0.0 0.005103236778959423 0.0012247768269502614 32-33 1.0206473557918845E-4 0.0 0.0 0.015258677969088676 0.0012247768269502614 34-35 1.0206473557918845E-4 0.0 0.0 0.03077251777712532 0.0012247768269502614 36-37 1.0206473557918845E-4 0.0 0.0 0.05514897879128816 0.0012247768269502614 38-39 1.0206473557918845E-4 0.0 0.0 0.09403564304695897 0.0012247768269502614 40-41 1.0206473557918845E-4 0.0 0.0 0.13564403358474147 0.0012587984054766577 42-43 1.0206473557918845E-4 0.0 0.0 0.17664003570904882 0.0012587984054766577 44-45 1.1907552484238653E-4 0.0 0.0 0.22865902927590853 0.0012758091947398557 46-47 1.360863141055846E-4 0.0 0.0 0.29051025903689676 0.0012928199840030537 48-49 1.530971033687827E-4 0.0 0.0 0.3574647255768444 0.0012928199840030537 50-51 1.7010789263198077E-4 0.0 0.0 0.4309343244045969 0.00132684156252945 52-53 1.7010789263198077E-4 0.0 0.0 0.5069725524110922 0.0013778739303190442 54-55 1.7010789263198077E-4 0.0 0.0 0.5925538331942417 0.0014629278766350345 56-57 1.7010789263198077E-4 0.0 0.0 0.6904509254039467 0.0015139602444246287 58-59 1.7010789263198077E-4 0.0 0.0 0.8003066024656799 0.001564992612214223 60-61 1.7010789263198077E-4 0.0 0.0 0.9194161488865928 0.001564992612214223 62-63 2.041294711583769E-4 0.0 0.0 1.0419448639494084 0.0015990141907406192 64-65 2.041294711583769E-4 0.0 0.0 1.1718562615524521 0.0016330357692670152 66-67 2.041294711583769E-4 0.0 0.0 1.3251745051816566 0.0016330357692670152 68-69 2.041294711583769E-4 0.0 0.0 1.4938534915155288 0.0016500465585302135 70-71 2.041294711583769E-4 0.0 0.0 1.675953990578064 0.0017180897155830057 72-73 2.3815104968477306E-4 0.0 0.0 1.8724966497250546 0.0019562407652677785 74-75 2.3815104968477306E-4 0.0 0.0 2.0774766603465915 0.0020753162901101653 76-77 2.3815104968477306E-4 3.402157852639615E-5 0.0 2.302342283616807 0.0020753162901101653 78-79 2.3815104968477306E-4 3.402157852639615E-5 0.0 2.562539316186685 0.0020923270793733635 80-81 2.3815104968477306E-4 3.402157852639615E-5 0.0 2.8521309926033687 0.0021263486578997595 82-83 2.3815104968477306E-4 3.402157852639615E-5 0.0 3.1549230414882943 0.002177381025689354 84-85 2.3815104968477306E-4 3.402157852639615E-5 0.0 3.471034538366304 0.00221140260421575 86-87 2.3815104968477306E-4 3.402157852639615E-5 0.0 3.8042078568753017 0.002262434972005344 88-89 2.3815104968477306E-4 3.402157852639615E-5 0.0 4.174872954920388 0.002330478129058136 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3195 0.0 54.088127 1 GTATCAA 5705 0.0 38.113712 1 TCAACGC 6995 0.0 30.943346 4 ATCAACG 7000 0.0 30.854483 3 CAACGCA 7140 0.0 30.306694 5 TATCAAC 7320 0.0 29.571005 2 AACGCAG 7535 0.0 29.1588 6 ACGCAGA 8840 0.0 24.585846 7 GTACATG 8260 0.0 24.542528 1 CGCAGAG 9115 0.0 23.792027 8 TACATGG 8485 0.0 23.105244 2 ACATGGG 8345 0.0 22.696114 3 GCAGAGT 10730 0.0 19.945675 9 TATCACG 295 3.0449883E-9 19.309523 2 CATGGGG 6300 0.0 19.288105 4 AGAGTAC 9545 0.0 18.693209 10-11 GTGGTAT 1715 0.0 18.547361 1 GAGTACA 6610 0.0 18.38696 1 CAGAGTA 10095 0.0 18.215342 10-11 GAGTACT 6295 0.0 17.56435 12-13 >>END_MODULE