FastQCFastQC Report
Sat 18 Jun 2016
SRR1930936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181645
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39160.17949758095382154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.052.3011131
GTATCAA30800.045.179561
TCAACGC36400.037.236144
ATCAACG37650.035.74493
CAACGCA38300.035.0809485
TATCAAC41000.032.95422
AACGCAG42850.031.824046
ATAACGC1959.094947E-1230.7874283
TAACGCA2800.028.5797674
ACGCAGA50200.027.2681037
GTACATG44750.026.0621811
CGCAGAG53400.025.6724628
TACATGG44750.025.6078572
ACATGGG45750.024.3859983
GCAGAGT59200.022.4936269
GTATAAC5200.021.1771371
CATGGGG35200.020.6026024
AGAGTAC55050.020.02632510-11
GTACTAG4150.019.2983341
TATAACG3255.2586984E-918.4768832
Produced by FastQC (version 0.11.5)