FastQCFastQC Report
Sat 18 Jun 2016
SRR1930936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2181645
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.15304964831583506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.1338439571974359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24750.051.030071
GTATCAA43800.039.242281
TAACGCA3000.030.066474
TCAACGC56000.030.0099534
ATCAACG55900.029.97943
CAACGCA56600.029.8506775
TATCACG1951.6370905E-1129.2150522
TATCAAC58300.029.070982
AACGCAG60350.027.9945496
GTGGTAT12950.024.9321021
GTACATG56950.023.9280831
ATAACGC3000.023.737233
ACGCAGA72800.023.141837
CGCAGAG74950.022.414678
ACATGGG57150.022.1796683
TACATGG60000.022.0756242
TGGTATC13900.021.51722
TATAACG3101.0913936E-1121.4400772
CATGGGG43050.020.4008674
AGAGTAC77650.019.92401510-11
Produced by FastQC (version 0.11.5)