FastQCFastQC Report
Sat 18 Jun 2016
SRR1930935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584168
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29170.18413451098620853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12100.048.2985271
GTATCAA21150.044.067931
TCAACGC28400.033.5106474
ATCAACG27750.033.212493
CAACGCA29250.032.7198075
GTACATG55250.032.3710941
TATCAAC29000.031.9573062
TACATGG57500.030.3956852
AACGCAG33650.029.4438536
ACATGGG61550.028.31143
TAGTATA2601.0913936E-1125.1773134
CATGGGG50150.024.7003754
ACGCAGA39100.024.658757
GTATAGA2501.8371793E-1024.1825731
CGCAGAG41750.023.758458
GTGGTAT6150.022.93741
ATGGGGG28800.021.9862025
ATACGGG3950.021.67533
TAATATA2356.1114406E-821.4275024
CTAGTAC5000.021.1525443
Produced by FastQC (version 0.11.5)