##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930935_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1584168 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.417981552461608 30.0 10.0 31.0 2.0 33.0 2 21.233521949692204 28.0 10.0 31.0 2.0 33.0 3 21.68622519833755 28.0 10.0 31.0 2.0 33.0 4 23.57994669757248 32.0 2.0 35.0 2.0 37.0 5 23.505156018806087 32.0 2.0 35.0 2.0 37.0 6 24.261582736174446 32.0 10.0 35.0 2.0 37.0 7 24.276688457284834 32.0 10.0 35.0 2.0 37.0 8 25.02163975032951 32.0 17.0 35.0 2.0 37.0 9 26.149984092596238 33.0 17.0 37.0 2.0 39.0 10-11 26.330628443447917 32.5 17.0 37.0 2.0 39.0 12-13 26.196000992318996 33.0 15.5 37.0 2.0 39.0 14-15 26.365956451588467 33.0 13.5 38.0 2.0 40.0 16-17 25.439105259038183 32.0 10.0 38.0 2.0 40.0 18-19 24.71801096853364 32.0 9.0 38.0 2.0 40.0 20-21 24.1482910903389 31.0 2.0 37.0 2.0 39.5 22-23 23.98604062195424 31.0 8.0 37.0 2.0 39.0 24-25 23.634937393003774 30.0 2.0 37.0 2.0 39.0 26-27 23.24300800167659 30.0 2.0 37.0 2.0 39.0 28-29 23.113009163169565 30.0 2.0 37.0 2.0 39.0 30-31 22.958965526383565 30.0 2.0 37.0 2.0 39.0 32-33 22.58534258992733 30.0 2.0 37.0 2.0 39.0 34-35 19.847273773993667 23.5 2.0 35.0 2.0 38.0 36-37 18.19197521979992 16.5 2.0 34.0 2.0 38.0 38-39 17.268561478328056 15.0 2.0 33.0 2.0 38.0 40-41 18.361085756056177 17.0 2.0 33.0 2.0 38.0 42-43 18.19614744143298 16.5 2.0 33.0 2.0 38.0 44-45 16.34003432716732 11.0 2.0 32.0 2.0 37.5 46-47 16.95201582155428 14.5 2.0 31.5 2.0 37.0 48-49 16.446196047388913 10.0 2.0 32.0 2.0 37.0 50-51 14.590948371637353 7.0 2.0 29.5 2.0 36.5 52-53 17.12984292070033 16.0 2.0 32.0 2.0 37.0 54-55 18.500231351725322 21.5 2.0 34.0 2.0 38.0 56-57 18.59667693072957 22.0 2.0 34.0 2.0 38.0 58-59 18.192398154741163 20.5 2.0 34.0 2.0 38.0 60-61 17.8076561324304 19.0 2.0 33.5 2.0 37.5 62-63 17.291973136687524 16.0 2.0 33.0 2.0 36.5 64-65 16.771275836906185 12.0 2.0 32.5 2.0 36.0 66-67 16.310337034960938 8.0 2.0 32.0 2.0 35.5 68-69 15.839955105771612 6.5 2.0 31.5 2.0 35.0 70-71 15.261748438296948 2.0 2.0 31.0 2.0 35.0 72-73 14.784450891572106 2.0 2.0 30.5 2.0 34.0 74-75 14.259550123471755 2.0 2.0 30.0 2.0 34.0 76-77 13.721100918589443 2.0 2.0 29.5 2.0 34.0 78-79 13.24093751420304 2.0 2.0 29.0 2.0 34.0 80-81 12.761971583821918 2.0 2.0 29.0 2.0 34.0 82-83 12.297542937365229 2.0 2.0 27.5 2.0 33.5 84-85 11.810211101347836 2.0 2.0 27.0 2.0 33.0 86-87 11.284829008034503 2.0 2.0 25.5 2.0 33.0 88-89 10.776892665424374 2.0 2.0 25.0 2.0 32.5 90-91 10.24176507794628 2.0 2.0 24.0 2.0 32.0 92-93 9.65600428742406 2.0 2.0 21.0 2.0 32.0 94-95 9.04251727089551 2.0 2.0 16.5 2.0 31.5 96-97 8.384649860368345 2.0 2.0 4.5 2.0 31.0 98-99 7.690406257417143 2.0 2.0 2.0 2.0 31.0 100-101 6.834385620716994 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.880525847142533 1101 2 7.889795884876101 1101 3 7.585185766168422 1101 4 9.581166480267122 1101 5 9.292102387551882 1101 6 8.765240505952576 1101 7 8.627699957458812 1101 8 7.444603030167279 1101 9 7.772424701216874 1101 10-11 7.299006418112327 1101 12-13 7.129324261602694 1101 14-15 7.573996678107854 1101 16-17 8.280683964256127 1101 18-19 8.662588902520831 1101 20-21 9.208297864012447 1101 22-23 9.244870961277414 1101 24-25 9.10818566762119 1101 26-27 9.162940680805562 1101 28-29 9.001972355278372 1101 30-31 8.831323521130283 1101 32-33 8.763186258649974 1101 34-35 11.093448974462987 1101 36-37 5.613539319289643 1101 38-39 8.299278981758722 1101 40-41 9.702483646120502 1101 42-43 10.08505433122819 1101 44-45 4.508608954579589 1101 46-47 6.102076643452413 1101 48-49 8.622202052343408 1101 50-51 -2.8923154368204127 1101 52-53 5.423501085682052 1101 54-55 7.975737838531039 1101 56-57 8.367217044222148 1101 58-59 8.746379109774718 1101 60-61 8.248445703281245 1101 62-63 8.353989455325063 1101 64-65 8.400074196101691 1101 66-67 8.312775878126711 1101 68-69 8.423922195785847 1101 70-71 8.371287687711686 1101 72-73 8.278839927925073 1101 74-75 8.223472675480139 1101 76-77 8.177108368023879 1101 78-79 7.838518187108862 1101 80-81 7.571810333844004 1101 82-83 7.565164532873025 1101 84-85 7.3688527922324365 1101 86-87 7.126988996247965 1101 88-89 6.84991631100128 1101 90-91 6.528033152566316 1101 92-93 6.386715998480906 1101 94-95 6.047833645386877 1101 96-97 5.978415100856498 1101 98-99 5.572910669412738 1101 100-101 5.152274334886925 1102 1 6.783867656671603 1102 2 7.710788432098678 1102 3 7.697411479300666 1102 4 9.516076997932167 1102 5 9.48510762028685 1102 6 8.68697156344421 1102 7 8.24548900053028 1102 8 7.407998388302751 1102 9 7.458350534374535 1102 10-11 6.990117536137838 1102 12-13 6.887680146698774 1102 14-15 7.054843755765006 1102 16-17 7.943924759519831 1102 18-19 8.263063714684549 1102 20-21 8.583766433765259 1102 22-23 8.211889445814514 1102 24-25 8.5021996428429 1102 26-27 8.450407008735539 1102 28-29 8.263113016826132 1102 30-31 8.051484797024617 1102 32-33 8.103372371949927 1102 34-35 10.417296112016164 1102 36-37 1.2785748563042247 1102 38-39 3.4683477774051354 1102 40-41 8.214528582144542 1102 42-43 9.102000434913663 1102 44-45 1.5137782931018364 1102 46-47 2.0114294474033727 1102 48-49 7.077641934231547 1102 50-51 -4.186121409465818 1102 52-53 4.6047958667153885 1102 54-55 7.286108369373217 1102 56-57 7.659047809156679 1102 58-59 7.795037438706601 1102 60-61 7.958716938951898 1102 62-63 8.078385652782828 1102 64-65 8.17645489682301 1102 66-67 7.812502456639901 1102 68-69 7.874964970107717 1102 70-71 7.5760445992857015 1102 72-73 7.412852044036249 1102 74-75 7.222477779088603 1102 76-77 7.012370560949767 1102 78-79 7.375612498463985 1102 80-81 7.000086588335234 1102 82-83 6.9883107313031285 1102 84-85 6.851241025851843 1102 86-87 6.4298797186640915 1102 88-89 6.247119555343634 1102 90-91 6.083745794751323 1102 92-93 6.153820593284262 1102 94-95 5.775964788133116 1102 96-97 5.326047379682631 1102 98-99 5.0893846056942555 1102 100-101 4.40023436945549 1103 1 6.443046886405625 1103 2 7.369802464427469 1103 3 7.073469574351549 1103 4 8.68702304604733 1103 5 8.599599250789023 1103 6 7.987949138936024 1103 7 7.736163096669877 1103 8 7.04909625169924 1103 9 6.855137312533703 1103 10-11 6.304881345431234 1103 12-13 6.233030614695085 1103 14-15 6.558107444090041 1103 16-17 7.355929167304147 1103 18-19 7.590189355438788 1103 20-21 7.961780435851967 1103 22-23 7.754734167814121 1103 24-25 8.010963232881156 1103 26-27 8.058621893891214 1103 28-29 7.8052173747110025 1103 30-31 7.828339771092029 1103 32-33 7.869544706487904 1103 34-35 9.820593066637912 1103 36-37 -2.9045188714867436 1103 38-39 -1.8133472538198205 1103 40-41 6.034695140443727 1103 42-43 8.476770217019418 1103 44-45 0.7368359950055776 1103 46-47 0.6573770076229302 1103 48-49 6.636891665833019 1103 50-51 -4.693994370015016 1103 52-53 3.9374198970784455 1103 54-55 6.6703422238785315 1103 56-57 7.300071957463878 1103 58-59 7.3331040998288834 1103 60-61 7.546382325370175 1103 62-63 7.40670925824455 1103 64-65 7.35562282357667 1103 66-67 7.363344320762202 1103 68-69 7.346065063858189 1103 70-71 7.176616741786418 1103 72-73 7.1685360444596355 1103 74-75 7.1457827100610345 1103 76-77 7.077374227802926 1103 78-79 6.941320616952483 1103 80-81 6.714276364997415 1103 82-83 6.462279476105271 1103 84-85 6.481761719000497 1103 86-87 6.222845409015354 1103 88-89 5.657431918309172 1103 90-91 5.5604711058667995 1103 92-93 5.338132132152907 1103 94-95 5.261998424895399 1103 96-97 4.7906301144746415 1103 98-99 4.124429704207855 1103 100-101 3.7832640218226894 1104 1 6.1681515624223735 1104 2 7.169027276303439 1104 3 6.710460468270092 1104 4 8.310008581725018 1104 5 8.21523550529082 1104 6 7.865264455306473 1104 7 7.436240688682293 1104 8 6.76759169865851 1104 9 6.76502224211529 1104 10-11 5.955665317556665 1104 12-13 5.943010338291835 1104 14-15 6.362148450736598 1104 16-17 6.782536273561284 1104 18-19 7.29265912383395 1104 20-21 7.644101169023283 1104 22-23 7.731165228043089 1104 24-25 7.619707340280211 1104 26-27 7.616096347087126 1104 28-29 7.422703759508824 1104 30-31 7.024779322522356 1104 32-33 6.78472712091709 1104 34-35 8.662217887297881 1104 36-37 -3.291431075392696 1104 38-39 -2.4429819125613665 1104 40-41 4.915706325405516 1104 42-43 6.943825819748316 1104 44-45 0.30515429793650384 1104 46-47 -0.33274684679688704 1104 48-49 5.784913435516504 1104 50-51 -4.930981969933031 1104 52-53 3.203950655723368 1104 54-55 5.774394942526968 1104 56-57 6.260328743104964 1104 58-59 6.458753017932711 1104 60-61 6.288679488137721 1104 62-63 6.402623965590902 1104 64-65 6.361935226586656 1104 66-67 6.204929979415905 1104 68-69 6.080736615405639 1104 70-71 6.121434546957268 1104 72-73 5.876983325811203 1104 74-75 5.616739141414065 1104 76-77 5.575020481426327 1104 78-79 5.486698036212873 1104 80-81 5.378068345204133 1104 82-83 5.128363714678747 1104 84-85 4.982523731122418 1104 86-87 4.70754807056778 1104 88-89 4.575925535268151 1104 90-91 4.097795059838633 1104 92-93 4.116780420736632 1104 94-95 3.971010444337322 1104 96-97 3.627057368303001 1104 98-99 3.1657178048139505 1104 100-101 3.0803272373528037 1105 1 5.695427564376608 1105 2 6.562654307742122 1105 3 6.512813069085965 1105 4 8.145753038988136 1105 5 8.37179358038146 1105 6 7.195323117873684 1105 7 7.2251096916881 1105 8 6.242306097117247 1105 9 6.399664994668175 1105 10-11 5.840864878775136 1105 12-13 5.466566659816312 1105 14-15 5.770036945768403 1105 16-17 6.362893460736213 1105 18-19 7.268552198133957 1105 20-21 7.429578975030509 1105 22-23 7.202957843494467 1105 24-25 7.253020736518803 1105 26-27 7.099556949284292 1105 28-29 6.932592830317297 1105 30-31 6.777445125996728 1105 32-33 6.446644457255051 1105 34-35 8.469834022942926 1105 36-37 -3.2866369545547567 1105 38-39 -2.6812417764596717 1105 40-41 4.299132997905584 1105 42-43 6.661128334374958 1105 44-45 -3.052821201773124 1105 46-47 -4.285997432323477 1105 48-49 3.851997445730156 1105 50-51 -8.844626636305192 1105 52-53 0.2254073778097201 1105 54-55 4.480514129717498 1105 56-57 5.4313535617743085 1105 58-59 5.586047172452165 1105 60-61 5.541403791089852 1105 62-63 5.699845630393856 1105 64-65 5.572996910563258 1105 66-67 5.4623340633176305 1105 68-69 5.289270767400756 1105 70-71 5.466214691738159 1105 72-73 5.370066737557472 1105 74-75 5.126529472194932 1105 76-77 4.958017803213876 1105 78-79 4.923356042593214 1105 80-81 4.873086494223925 1105 82-83 4.627198511444471 1105 84-85 4.634330762263287 1105 86-87 4.380889291285246 1105 88-89 4.292720877149737 1105 90-91 4.147972366437585 1105 92-93 3.874471506621667 1105 94-95 3.348936570487723 1105 96-97 3.180683130480288 1105 98-99 3.072045604687591 1105 100-101 2.9062611549245583 1106 1 5.4595263857329925 1106 2 6.513370049383894 1106 3 6.367796064379039 1106 4 7.812069837882156 1106 5 7.455534502587582 1106 6 6.7789241637941835 1106 7 6.26349245269725 1106 8 5.703273885202194 1106 9 5.503318279954886 1106 10-11 5.41412711954365 1106 12-13 5.41484979349838 1106 14-15 5.264040502521347 1106 16-17 5.869057932584603 1106 18-19 6.487966743284609 1106 20-21 6.724806112871331 1106 22-23 6.626726330181789 1106 24-25 6.751732812339544 1106 26-27 6.621780635190856 1106 28-29 6.612829090216646 1106 30-31 6.05757589438851 1106 32-33 6.358140658994191 1106 34-35 8.26776874073278 1106 36-37 -0.44022908380469516 1106 38-39 0.5252157981776051 1106 40-41 5.693623607426236 1106 42-43 6.892473264337664 1106 44-45 -3.108927881697939 1106 46-47 -4.2482745686770045 1106 48-49 3.7289392472800564 1106 50-51 -8.833129159744207 1106 52-53 -0.00802082351581035 1106 54-55 4.159662718702187 1106 56-57 5.305561224586764 1106 58-59 5.74285946626744 1106 60-61 5.47092756722547 1106 62-63 5.592547416985848 1106 64-65 5.549854998812094 1106 66-67 5.2787724660508495 1106 68-69 5.248526753043427 1106 70-71 5.004482010547019 1106 72-73 4.971032096398574 1106 74-75 4.860092238048402 1106 76-77 4.7033473517427975 1106 78-79 4.435850359012493 1106 80-81 4.205942300541663 1106 82-83 3.9690701411342193 1106 84-85 3.7607127045535194 1106 86-87 3.7735604467412713 1106 88-89 3.9193967366983635 1106 90-91 3.465287740364081 1106 92-93 3.2289360743649915 1106 94-95 3.111956750917507 1106 96-97 2.705052235314554 1106 98-99 2.69653372830565 1106 100-101 2.4015172241388862 1107 1 5.821965244306856 1107 2 6.521975356052877 1107 3 6.284011797945148 1107 4 7.579700012063686 1107 5 7.935122540035714 1107 6 6.359473985006794 1107 7 6.459956558008745 1107 8 5.763108019045205 1107 9 5.873958053357558 1107 10-11 5.478420595631096 1107 12-13 5.111802582043854 1107 14-15 5.401102962961275 1107 16-17 6.167735609189727 1107 18-19 6.624728409282458 1107 20-21 7.0762336539896715 1107 22-23 6.857129012061584 1107 24-25 6.841515730111134 1107 26-27 6.922347542217274 1107 28-29 6.9550271546518125 1107 30-31 6.836435957574306 1107 32-33 6.57889792322306 1107 34-35 8.75919721957133 1107 36-37 -0.20078722728415954 1107 38-39 0.6959272451212577 1107 40-41 5.980490663522591 1107 42-43 7.036869937913121 1107 44-45 -3.06454165404981 1107 46-47 -4.189652403729262 1107 48-49 4.078001113370853 1107 50-51 -8.829070072339032 1107 52-53 0.03187038025492939 1107 54-55 4.446834706241539 1107 56-57 5.420100990129132 1107 58-59 5.746544155089126 1107 60-61 5.805429086194312 1107 62-63 5.604130330068838 1107 64-65 5.728054589800603 1107 66-67 5.815153252163764 1107 68-69 5.549770059720684 1107 70-71 5.484830566664748 1107 72-73 5.391001296426708 1107 74-75 5.492571935716377 1107 76-77 5.241809738819912 1107 78-79 5.074128580598542 1107 80-81 5.098092582616173 1107 82-83 5.089484275611573 1107 84-85 4.826371390945406 1107 86-87 4.632772616970586 1107 88-89 4.425818396380393 1107 90-91 4.242383545031444 1107 92-93 4.1202367198750345 1107 94-95 3.8365038527415116 1107 96-97 3.7437816313262093 1107 98-99 3.4323279566377627 1107 100-101 2.883339759210311 1108 1 5.864627621200313 1108 2 6.15469749375751 1108 3 6.59060308918497 1108 4 7.815632465340602 1108 5 7.9606176627568 1108 6 7.17219090828122 1108 7 7.54027631904491 1108 8 6.78476312419075 1108 9 4.910131839464011 1108 10-11 5.739447990579848 1108 12-13 5.241063046141374 1108 14-15 5.990637475805784 1108 16-17 6.172612557239312 1108 18-19 6.687268981586023 1108 20-21 7.756684867635066 1108 22-23 7.514049613097459 1108 24-25 7.190400980221806 1108 26-27 7.531418632503019 1108 28-29 7.247298614712122 1108 30-31 7.181780788240982 1108 32-33 7.0970609151942945 1108 34-35 9.5014685472857 1108 36-37 -3.0635960979034262 1108 38-39 -2.1694858628181084 1108 40-41 5.015175226890161 1108 42-43 7.061421764915796 1108 44-45 -2.8287966357165875 1108 46-47 -4.075617182503089 1108 48-49 4.389552068875485 1108 50-51 -8.81799834149892 1108 52-53 0.5555252326197184 1108 54-55 4.932944668516328 1108 56-57 6.1605704278110665 1108 58-59 6.567512517129398 1108 60-61 6.3767880342028675 1108 62-63 6.51656148536291 1108 64-65 6.698072443268497 1108 66-67 6.467642083820383 1108 68-69 6.53008194617316 1108 70-71 6.394584198072028 1108 72-73 6.174992863343132 1108 74-75 6.18628272409455 1108 76-77 6.229377160806939 1108 78-79 6.229907863296754 1108 80-81 6.180240537441213 1108 82-83 6.024646508578817 1108 84-85 5.966235742969735 1108 86-87 5.7431701444836545 1108 88-89 5.6830939492390335 1108 90-91 5.415516855116007 1108 92-93 5.446022474289931 1108 94-95 5.2955465879086745 1108 96-97 5.118102117618147 1108 98-99 4.887774521631864 1108 100-101 4.567275641570649 1109 1 4.7817914595402975 1109 2 6.454684768158845 1109 3 6.041594222777217 1109 4 7.310290776211328 1109 5 7.6056755370101925 1109 6 5.034871150734496 1109 7 4.886092421214453 1109 8 3.275559522097762 1109 9 2.5592105265360985 1109 10-11 2.5360488606841542 1109 12-13 3.117671864640993 1109 14-15 3.9154892429967347 1109 16-17 2.9813692662497857 1109 18-19 3.710703988142768 1109 20-21 4.155749944441201 1109 22-23 5.585363323603019 1109 24-25 5.445834538646256 1109 26-27 4.165190023587883 1109 28-29 4.138619280063583 1109 30-31 4.374902636188448 1109 32-33 4.000381922163431 1109 34-35 6.142546269389928 1109 36-37 -4.420460333459021 1109 38-39 -3.443175721604794 1109 40-41 2.7064072894102793 1109 42-43 3.1023410294986853 1109 44-45 -4.365374055332657 1109 46-47 -5.8887805553719765 1109 48-49 1.2585610849961633 1109 50-51 -9.169905037681715 1109 52-53 -1.5193523260252135 1109 54-55 1.7855322182533442 1109 56-57 3.166063361020729 1109 58-59 4.159001065111369 1109 60-61 4.297770232655516 1109 62-63 4.450467822234682 1109 64-65 3.968899892997282 1109 66-67 4.115997895642128 1109 68-69 4.131401529508478 1109 70-71 4.0538944025730785 1109 72-73 4.147188451031013 1109 74-75 4.4089698125116 1109 76-77 4.504863914955861 1109 78-79 4.5092148489699095 1109 80-81 4.588696357497161 1109 82-83 4.414424620413007 1109 84-85 4.130359388152826 1109 86-87 3.9939809760777063 1109 88-89 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2313 78-79 -11.152868228205312 2313 80-81 -10.64235944198753 2313 82-83 -10.185284059968005 2313 84-85 -9.720079985829267 2313 86-87 -9.198838059169058 2313 88-89 -8.679800330363044 2313 90-91 -8.12551694042638 2313 92-93 -7.557012131677254 2313 94-95 -6.979769867787528 2313 96-97 -6.30962666343807 2313 98-99 -5.607107342041432 2313 100-101 -4.761830861639642 2314 1 -20.325245796521205 2314 2 -19.149099974596922 2314 3 -19.571652376867963 2314 4 -21.47090378322096 2314 5 -21.37540074921098 2314 6 -22.142538665942027 2314 7 -22.135788122842317 2314 8 -22.873464723910516 2314 9 -23.99608219966142 2314 10-11 -24.187191825300474 2314 12-13 -24.04288401945126 2314 14-15 -24.209880360788006 2314 16-17 -23.308400100988536 2314 18-19 -22.570786254317312 2314 20-21 -22.047975661709007 2314 22-23 -21.87087558828344 2314 24-25 -21.521048962240794 2314 26-27 -21.14320737440147 2314 28-29 -21.01277043016705 2314 30-31 -20.872879741103095 2314 32-33 -20.48838212278039 2314 34-35 -17.808370347996235 2314 36-37 -16.13897009099894 2314 38-39 -15.216091156258845 2314 40-41 -16.30128046931237 2314 42-43 -16.13420539273668 2314 44-45 -14.289383517189545 2314 46-47 -14.882866894816093 2314 48-49 -14.397559553679177 2314 50-51 -12.57173827245404 2314 52-53 -15.080263029750821 2314 54-55 -16.41227972734098 2314 56-57 -16.515781701072708 2314 58-59 -16.096469070902824 2314 60-61 -15.716422552678605 2314 62-63 -15.201217571023744 2314 64-65 -14.662974737398937 2314 66-67 -14.210436167042676 2314 68-69 -13.732288594678396 2314 70-71 -13.166980819189192 2314 72-73 -12.68634200432085 2314 74-75 -12.17677826554872 2314 76-77 -11.650369674636098 2314 78-79 -11.170264748901173 2314 80-81 -10.675052903295269 2314 82-83 -10.222476621455707 2314 84-85 -9.757872427340965 2314 86-87 -9.238130200740743 2314 88-89 -8.71909247193473 2314 90-91 -8.163309381938078 2314 92-93 -7.5954044532129465 2314 94-95 -6.979769867787528 2314 96-97 -6.325223544061945 2314 98-99 -5.633802003109218 2314 100-101 -4.797223783055359 2315 1 -20.325245796521205 2315 2 -19.149099974596922 2315 3 -19.571652376867963 2315 4 -16.700775428495174 2315 5 -21.37540074921098 2315 6 -22.142538665942027 2315 7 -22.135788122842317 2315 8 -22.873464723910516 2315 9 -19.60284999429386 2315 10-11 -17.93310781129814 2315 12-13 -14.780631977444258 2315 14-15 -14.691794013063385 2315 16-17 -19.423919354197402 2315 18-19 -22.570786254317312 2315 20-21 -22.047975661709007 2315 22-23 -21.87087558828344 2315 24-25 -21.521048962240794 2315 26-27 -21.14320737440147 2315 28-29 -21.01277043016705 2315 30-31 -20.872879741103095 2315 32-33 -20.48838212278039 2315 34-35 -17.808370347996235 2315 36-37 -16.13897009099894 2315 38-39 -15.216091156258845 2315 40-41 -16.30128046931237 2315 42-43 -16.13420539273668 2315 44-45 -14.289383517189545 2315 46-47 -14.882866894816093 2315 48-49 -14.397559553679177 2315 50-51 -12.57173827245404 2315 52-53 -15.080263029750821 2315 54-55 -16.41227972734098 2315 56-57 -16.515781701072708 2315 58-59 -16.096469070902824 2315 60-61 -15.716422552678605 2315 62-63 -15.201217571023744 2315 64-65 -14.662974737398937 2315 66-67 -14.210436167042676 2315 68-69 -13.732288594678396 2315 70-71 -13.166980819189192 2315 72-73 -12.68634200432085 2315 74-75 -12.17677826554872 2315 76-77 -11.650369674636098 2315 78-79 -11.170264748901173 2315 80-81 -10.675052903295269 2315 82-83 -10.222476621455707 2315 84-85 -9.757872427340965 2315 86-87 -9.238130200740743 2315 88-89 -8.71909247193473 2315 90-91 -8.163309381938078 2315 92-93 -7.5954044532129465 2315 94-95 -6.979769867787528 2315 96-97 -6.325223544061945 2315 98-99 -5.633802003109218 2315 100-101 -4.797223783055359 2316 1 -20.325245796521205 2316 2 -19.149099974596922 2316 3 -19.571652376867963 2316 4 -21.47090378322096 2316 5 -21.37540074921098 2316 6 -22.142538665942027 2316 7 -22.135788122842317 2316 8 -22.873464723910516 2316 9 -23.99608219966142 2316 10-11 -24.187191825300474 2316 12-13 -24.04288401945126 2316 14-15 -24.209880360788006 2316 16-17 -23.308400100988536 2316 18-19 -22.570786254317312 2316 20-21 -22.047975661709007 2316 22-23 -21.87087558828344 2316 24-25 -21.521048962240794 2316 26-27 -21.14320737440147 2316 28-29 -21.01277043016705 2316 30-31 -20.872879741103095 2316 32-33 -20.48838212278039 2316 34-35 -17.808370347996235 2316 36-37 -16.13897009099894 2316 38-39 -15.216091156258845 2316 40-41 -16.30128046931237 2316 42-43 -16.13420539273668 2316 44-45 -14.289383517189545 2316 46-47 -14.882866894816093 2316 48-49 -14.397559553679177 2316 50-51 -12.57173827245404 2316 52-53 -15.080263029750821 2316 54-55 -16.41227972734098 2316 56-57 -16.515781701072708 2316 58-59 -16.096469070902824 2316 60-61 -15.716422552678605 2316 62-63 -15.201217571023744 2316 64-65 -14.662974737398937 2316 66-67 -14.210436167042676 2316 68-69 -13.732288594678396 2316 70-71 -13.166980819189192 2316 72-73 -12.68634200432085 2316 74-75 -12.17677826554872 2316 76-77 -11.650369674636098 2316 78-79 -11.170264748901173 2316 80-81 -10.675052903295269 2316 82-83 -10.222476621455707 2316 84-85 -9.757872427340965 2316 86-87 -9.238130200740743 2316 88-89 -8.71909247193473 2316 90-91 -8.163309381938078 2316 92-93 -7.5954044532129465 2316 94-95 -6.979769867787528 2316 96-97 -6.325223544061945 2316 98-99 -5.633802003109218 2316 100-101 -4.797223783055359 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 281866.0 3 19052.0 4 39468.0 5 26282.0 6 24075.0 7 29460.0 8 37094.0 9 43157.0 10 44487.0 11 40625.0 12 34071.0 13 29393.0 14 27408.0 15 28720.0 16 31394.0 17 33435.0 18 34873.0 19 36260.0 20 37881.0 21 39818.0 22 41707.0 23 43952.0 24 45788.0 25 47524.0 26 49569.0 27 51106.0 28 52962.0 29 54851.0 30 54386.0 31 52145.0 32 48932.0 33 44215.0 34 36556.0 35 25580.0 36 12855.0 37 3030.0 38 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.084296975958 16.277173611188253 11.811203803472447 25.827325609381308 2 17.951774171421192 18.606002404835493 36.6760815707288 26.76614185301452 3 21.382093826638382 22.4415182836527 26.215085842647735 29.961302047061185 4 14.745705872730246 14.360788483559627 32.81989203337151 38.073613610338626 5 16.443482650955556 35.66442992495723 31.419358844279316 16.472728579807892 6 36.4227045832703 31.526622296173045 16.102783240054457 15.947889880502194 7 32.03809484027945 29.29644785306276 18.97469444017621 19.690762866481585 8 29.50651728811604 32.19612423220002 18.167589468523808 20.129769011160136 9 28.998661805799536 14.241893104812611 17.510919677712256 39.2485254116756 10-11 28.868448992259392 23.422172087052434 24.764652945379176 22.944725975308994 12-13 28.770262692787053 20.765206585000325 25.063553649373933 25.400977072838693 14-15 25.828551268543393 21.863579707955036 22.6900285317141 29.617840491787472 16-17 26.403542309245402 24.518155444628686 23.156497097477224 25.921805148648687 18-19 26.359503588061926 24.102316799252154 24.45539173772 25.082787874965916 20-21 27.27583374306411 23.274299770724856 24.311633643236533 25.138232842974507 22-23 27.053172098115493 22.886044398891194 24.17847637960353 25.88230712338978 24-25 26.659066628253836 23.19654764691407 24.13781202921776 26.00657369561434 26-27 26.505934145335935 23.428693380882248 24.100736979041475 25.96463549474034 28-29 26.347321972900694 23.7338126953436 24.168365095439366 25.750500236316338 30-31 26.71306274882982 23.475362281494515 24.07161513216625 25.739959837509414 32-33 26.453360937859433 23.55155810767212 23.944322191695207 26.050758762773242 34-35 26.710545303961 23.57948663249236 24.156021182213465 25.55394688133317 36-37 26.981729193574765 23.699108236464184 23.722887484939807 25.59627508502124 38-39 26.94193861617934 23.67076811036662 23.71816683776016 25.669126435693872 40-41 26.987492575386447 23.316384176415788 23.979662560510118 25.716460687687647 42-43 26.91037358821109 23.336905988086766 23.833090834069733 25.919629589632407 44-45 26.927868090477762 23.6368826010389 23.739538182449376 25.69571112603396 46-47 26.54334162198443 23.487945339260826 23.83273819630759 26.13597484244715 48-49 26.89335493960981 23.678856126905384 23.59246331737085 25.835325616113952 50-51 26.783793373282176 23.815177472171868 23.766124085736298 25.634905068809662 52-53 26.28425241655168 23.580172077839737 23.967734779948326 26.16784072566026 54-55 25.8904207277051 23.446266221717053 24.204395454062542 26.458917596515306 56-57 26.153380849480467 23.599758765289263 23.813317328198956 26.433543057031315 58-59 26.21928220347114 23.65910267743686 23.882777819687366 26.238837299404633 60-61 26.231773993150426 23.634304320120116 23.984712122931526 26.14920956379794 62-63 26.113542007081968 23.717951281185375 23.833693999372578 26.33481271236008 64-65 25.99828997604273 23.505284106530887 23.93912079151363 26.55730512591275 66-67 26.225464440975454 23.680125317002833 23.65744808158894 26.436962160432774 68-69 26.025215553550435 23.637014143411953 23.79890999869533 26.538860304342283 70-71 25.81764937017892 23.47117533648037 23.90609502301321 26.805080270327498 72-73 26.08502725397261 23.672883787661405 23.661103303069385 26.580985655296598 74-75 26.118474168173016 23.61620571150947 23.722412351835843 26.542907768481673 76-77 26.137801811278578 23.36342840890332 23.640165420486614 26.858604359331483 78-79 26.050222590114714 23.501294763012062 23.542359605978525 26.9061230408947 80-81 26.17739690910423 23.610662312240503 23.500257790071277 26.71168298858399 82-83 26.371644258301036 23.561157107900645 23.675089577595987 26.392109056202333 84-85 26.25913763694556 23.525232817152776 23.37077352339947 26.84485602250219 86-87 25.867636186983354 23.68356760504804 23.574510691390472 26.874285516578134 88-89 26.07743685426372 23.380780785307188 23.48241544531018 27.05936691511891 90-91 26.110427873402 23.49947574533253 23.370634974045295 27.019461407220174 92-93 26.338363701393313 23.227621596577283 23.50929032046198 26.924724381567422 94-95 26.16532720190002 23.123844282933828 23.63346727981 27.07736123535615 96-97 25.9770532357965 23.20260474093836 23.548309267148333 27.27203275611681 98-99 25.87220906405514 23.02110333448652 23.474962521400503 27.63172508005784 100-101 26.70402873772281 23.04339362040742 22.811810828937794 27.440766812931976 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17379.0 1 20256.0 2 15439.0 3 7095.0 4 8573.0 5 12375.0 6 14324.0 7 14175.0 8 13195.5 9 12254.0 10 11463.0 11 10482.5 12 9440.0 13 8326.0 14 7175.5 15 6021.0 16 4869.0 17 4016.0 18 3347.0 19 2835.0 20 2559.5 21 2515.0 22 2588.5 23 2804.0 24 3144.5 25 3580.0 26 4076.5 27 4516.0 28 4972.0 29 5460.5 30 6026.0 31 6585.5 32 7213.0 33 7981.5 34 8749.0 35 9741.5 36 10933.0 37 12195.5 38 13657.5 39 15327.5 40 17369.0 41 20261.0 42 23724.0 43 27864.5 44 32939.5 45 38658.5 46 44840.0 47 51058.0 48 56959.0 49 62169.5 50 66089.5 51 69586.5 52 72419.0 53 73438.5 54 72725.0 55 70201.0 56 65984.5 57 60481.0 58 54945.5 59 49144.5 60 42903.5 61 36672.5 62 30600.5 63 24984.0 64 19854.5 65 15443.0 66 11966.5 67 9396.0 68 7384.5 69 5749.0 70 4539.0 71 3587.5 72 2847.0 73 2247.5 74 1670.5 75 1265.0 76 958.5 77 721.5 78 516.0 79 351.5 80 249.0 81 154.0 82 101.0 83 64.0 84 33.0 85 26.5 86 20.5 87 11.0 88 6.0 89 3.0 90 2.5 91 1.0 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.75998757707516 2 21.777866993904688 3 17.607412850152258 4 22.82384191575641 5 22.72902873937613 6 16.536629953388783 7 15.37172825104408 8 12.441104731316376 9 3.1569252756020827 10-11 6.743508264275001 12-13 3.6920642255114355 14-15 7.675006691209518 16-17 11.832835911342736 18-19 14.916063195317669 20-21 15.39337999505103 22-23 13.283944632134975 24-25 15.861954035178089 26-27 13.336085566682321 28-29 13.386616823468216 30-31 14.26209215184248 32-33 12.737664187131667 34-35 19.514091939743764 36-37 24.343630221037163 38-39 23.2895437857601 40-41 18.486360032521805 42-43 18.458364264396202 44-45 23.8118368758869 46-47 13.192697996677119 48-49 21.81832987410426 50-51 21.630502572959433 52-53 11.63983239151403 54-55 13.251751076905984 56-57 12.758211250321935 58-59 13.811350816327561 60-61 13.223597497235144 62-63 13.174328732811166 64-65 14.24769973891658 66-67 12.594592240216947 68-69 13.635201569530505 70-71 12.204292726529005 72-73 11.586302715368573 74-75 11.648890774210816 76-77 11.667007539604384 78-79 12.072520086253478 80-81 12.092278091717546 82-83 10.54815524616076 84-85 10.478370980855566 86-87 10.600832739961923 88-89 12.053172390807037 90-91 9.483053565025932 92-93 11.577623080380365 94-95 11.653056999005155 96-97 11.175677074653699 98-99 11.04873346766252 100-101 11.152857525211973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1584168.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.07131154481151 #Duplication Level Percentage of deduplicated Percentage of total 1 98.57406139759794 95.68713424163609 2 1.0441571375450356 2.027154056007455 3 0.19651515639588016 0.5722795190934552 4 0.07958997836118573 0.30903614341373864 5 0.03529064470543182 0.17128545834091127 6 0.016166511580488194 0.09415826893334235 7 0.010761027365618915 0.07312109279651653 8 0.006364651543931325 0.049426005831609866 9 0.006121218737726295 0.05347752580113904 >10 0.02846826576922906 0.4778994200124099 >50 0.0013921409101151387 0.09342280899735957 >100 0.001046479051987282 0.20644785333988566 >500 0.0 0.0 >1k 6.539043551103682E-5 0.18515760579612234 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2917 0.18413451098620853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2624923619212103E-4 2 0.0 0.0 0.0 0.0 1.2624923619212103E-4 3 0.0 0.0 0.0 0.0 1.2624923619212103E-4 4 0.0 0.0 0.0 0.0 1.2624923619212103E-4 5 0.0 0.0 0.0 0.0 1.8937385428818157E-4 6 0.0 0.0 0.0 0.0 3.1562309048030257E-4 7 0.0 0.0 0.0 0.0 3.1562309048030257E-4 8 6.312461809606051E-5 0.0 0.0 0.0 3.1562309048030257E-4 9 6.312461809606051E-5 0.0 0.0 0.0 3.1562309048030257E-4 10-11 6.312461809606051E-5 0.0 0.0 6.312461809606051E-5 3.1562309048030257E-4 12-13 6.312461809606051E-5 0.0 0.0 6.312461809606051E-5 8.206200352487867E-4 14-15 6.312461809606051E-5 0.0 0.0 6.312461809606051E-5 0.0010731185076330288 16-17 6.312461809606051E-5 0.0 0.0 6.312461809606051E-5 0.0013887415981133314 18-19 6.312461809606051E-5 0.0 0.0 9.468692714409077E-5 0.0015149908343054525 20-21 6.312461809606051E-5 0.0 0.0 1.578115452401513E-4 0.001609677761449543 22-23 6.312461809606051E-5 0.0 0.0 2.8406078143227234E-4 0.0017674893066896946 24-25 6.312461809606051E-5 6.312461809606051E-5 0.0 5.049969447684841E-4 0.0017674893066896946 26-27 6.312461809606051E-5 6.312461809606051E-5 0.0 8.52182344296817E-4 0.0018621762338337853 28-29 6.312461809606051E-5 6.312461809606051E-5 0.0 0.0019884254700259065 0.0018937385428818155 30-31 6.312461809606051E-5 6.312461809606051E-5 0.0 0.007764328025815444 0.0018937385428818155 32-33 6.312461809606051E-5 6.312461809606051E-5 0.0 0.021115184753132246 0.0018937385428818155 34-35 6.312461809606051E-5 6.312461809606051E-5 0.0 0.03992632094575828 0.0018937385428818155 36-37 6.312461809606051E-5 6.312461809606051E-5 0.0 0.07066800995853975 0.0019253008519298457 38-39 6.312461809606051E-5 6.312461809606051E-5 0.0 0.11450805722625379 0.0020831123971699972 40-41 6.312461809606051E-5 6.312461809606051E-5 0.0 0.16257745390640388 0.0020831123971699972 42-43 6.312461809606051E-5 6.312461809606051E-5 0.0 0.21052060135036182 0.002209361633362118 44-45 6.312461809606051E-5 6.312461809606051E-5 0.0 0.26831118921730523 0.0023040485605062087 46-47 9.468692714409077E-5 6.312461809606051E-5 0.0 0.3363595275248584 0.00246186010574636 48-49 1.2624923619212103E-4 6.312461809606051E-5 0.0 0.40787971982769505 0.0026512339600345417 50-51 1.2624923619212103E-4 6.312461809606051E-5 0.0 0.4916776503502154 0.0027143585781306025 52-53 1.2624923619212103E-4 6.312461809606051E-5 0.0 0.5967801394801562 0.002935294741466814 54-55 1.2624923619212103E-4 6.312461809606051E-5 0.0 0.7118878805783224 0.0029668570505148443 56-57 1.2624923619212103E-4 6.312461809606051E-5 0.0 0.8350755727927845 0.0030299816686109047 58-59 1.2624923619212103E-4 6.312461809606051E-5 0.0 0.9670691492316471 0.0030931062867069654 60-61 1.2624923619212103E-4 6.312461809606051E-5 0.0 1.1091942268749273 0.0030931062867069654 62-63 1.2624923619212103E-4 6.312461809606051E-5 0.0 1.251855863772024 0.003282480140995147 64-65 1.2624923619212103E-4 6.312461809606051E-5 0.0 1.3942018775786407 0.003282480140995147 66-67 1.578115452401513E-4 6.312461809606051E-5 0.0 1.5461743956449063 0.0033771670681392377 68-69 1.8937385428818157E-4 6.312461809606051E-5 0.0 1.7153799344513967 0.0034718539952833284 70-71 2.5249847238424205E-4 6.312461809606051E-5 0.0 1.9033019225233687 0.003534978613379389 72-73 2.5249847238424205E-4 1.2624923619212103E-4 0.0 2.089424858979603 0.00362966554052348 74-75 2.5249847238424205E-4 1.2624923619212103E-4 0.0 2.2681622151185987 0.0037243524676675707 76-77 2.5249847238424205E-4 1.2624923619212103E-4 0.0 2.468235692174062 0.003787477085763631 78-79 2.5249847238424205E-4 1.2624923619212103E-4 0.0 2.690118724781715 0.003787477085763631 80-81 2.5249847238424205E-4 1.2624923619212103E-4 0.0 2.9256051125890687 0.0038190393948116614 82-83 2.5249847238424205E-4 1.2624923619212103E-4 0.0 3.1672461506607883 0.003945288631003782 84-85 2.5249847238424205E-4 1.2624923619212103E-4 0.0 3.410907176511582 0.003976850940051813 86-87 2.5249847238424205E-4 1.2624923619212103E-4 0.0 3.6541894546537996 0.004071537867195904 88-89 2.5249847238424205E-4 1.2624923619212103E-4 0.0 3.9080766686361548 0.0041662247943399944 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1210 0.0 48.298527 1 GTATCAA 2115 0.0 44.06793 1 TCAACGC 2840 0.0 33.510647 4 ATCAACG 2775 0.0 33.21249 3 CAACGCA 2925 0.0 32.719807 5 GTACATG 5525 0.0 32.371094 1 TATCAAC 2900 0.0 31.957306 2 TACATGG 5750 0.0 30.395685 2 AACGCAG 3365 0.0 29.443853 6 ACATGGG 6155 0.0 28.3114 3 TAGTATA 260 1.0913936E-11 25.177313 4 CATGGGG 5015 0.0 24.700375 4 ACGCAGA 3910 0.0 24.65875 7 GTATAGA 250 1.8371793E-10 24.182573 1 CGCAGAG 4175 0.0 23.75845 8 GTGGTAT 615 0.0 22.9374 1 ATGGGGG 2880 0.0 21.986202 5 ATACGGG 395 0.0 21.6753 3 TAATATA 235 6.1114406E-8 21.427502 4 CTAGTAC 500 0.0 21.152544 3 >>END_MODULE