FastQCFastQC Report
Sat 18 Jun 2016
SRR1930935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1584168
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.052.862431
GTATCAA31250.042.0805471
GTGGTAT8250.030.4981541
CAACGCA41950.030.4303825
TCAACGC43050.029.8799044
ATCAACG43950.029.2689533
TATCAAC44850.029.104962
AACGCAG44850.028.7801826
TGGTATC8350.027.2867342
GTACATG78600.026.4547211
GTACTAG3150.025.6206421
TATTACG950.001233471324.9766125
TACATGG82000.024.833632
TAGTACT7200.024.3922754
ACATGGG82400.024.252233
CTAGTAC7050.024.2387473
TAATACC2351.7826096E-1024.2379824
ACGCAGA53550.023.9271747
GAGTACA58000.022.4271551
CGCAGAG56950.022.4153568
Produced by FastQC (version 0.11.5)