##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930935_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1584168 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.217808969755733 31.0 31.0 33.0 30.0 34.0 2 31.325830972472616 31.0 31.0 34.0 30.0 34.0 3 31.413568510410514 31.0 31.0 34.0 28.0 34.0 4 35.28212159316436 37.0 35.0 37.0 33.0 37.0 5 35.04783962307028 35.0 35.0 37.0 32.0 37.0 6 35.088060735982545 36.0 35.0 37.0 32.0 37.0 7 34.948660748102476 36.0 35.0 37.0 32.0 37.0 8 34.92974103756672 36.0 35.0 37.0 32.0 37.0 9 36.428535988607265 38.0 35.0 39.0 32.0 39.0 10-11 36.34763958115553 38.0 35.0 39.0 32.0 39.0 12-13 36.21727872296373 38.0 35.0 39.0 32.0 39.0 14-15 37.226117431989536 39.0 36.0 40.0 31.0 41.0 16-17 37.158803548613534 39.0 36.0 40.0 31.0 41.0 18-19 37.096046315794794 39.0 36.0 40.0 31.0 41.0 20-21 37.02301712949637 39.0 36.0 40.0 31.0 41.0 22-23 36.87550247196005 39.0 36.0 40.0 31.0 41.0 24-25 36.720365832411716 38.0 35.0 40.0 30.0 41.0 26-27 36.40873884587998 38.0 35.0 40.0 30.0 41.0 28-29 36.19854333631282 38.0 35.0 40.0 30.0 41.0 30-31 35.94822045389125 38.0 34.0 40.0 29.0 41.0 32-33 35.70964916599755 38.0 34.0 40.0 28.5 41.0 34-35 35.97450301988173 38.0 34.5 40.0 29.5 41.0 36-37 36.049487175602586 38.0 35.0 40.0 29.5 41.0 38-39 36.002679324415084 38.0 35.0 40.0 29.0 41.0 40-41 35.878584847061674 38.0 34.0 40.0 29.0 41.0 42-43 35.71640034390292 38.0 34.0 40.0 28.0 41.0 44-45 35.510068692209416 38.0 34.0 40.0 27.5 41.0 46-47 35.25268595249999 38.0 33.5 40.0 27.0 41.0 48-49 35.00500988531519 38.0 33.0 40.0 26.5 41.0 50-51 34.65860723105125 37.0 33.0 40.0 26.0 41.0 52-53 34.373748554446244 37.0 33.0 40.0 26.0 41.0 54-55 34.10482915953359 36.5 32.5 39.5 25.5 41.0 56-57 33.74576149751794 36.0 32.0 39.0 24.5 41.0 58-59 33.401749056918206 35.5 31.5 39.0 24.0 40.5 60-61 32.991552032360204 35.0 31.0 39.0 23.0 40.0 62-63 32.598347208124395 35.0 31.0 38.0 22.5 40.0 64-65 32.21019740330571 35.0 31.0 37.5 22.0 40.0 66-67 31.80437207417395 34.5 30.0 37.0 22.0 39.0 68-69 31.36357191913989 34.0 30.0 36.0 20.5 39.0 70-71 30.932574701673055 34.0 30.0 36.0 20.0 39.0 72-73 30.508684053711477 34.0 29.0 35.0 19.0 37.5 74-75 30.00676822155226 34.0 29.0 35.0 18.0 37.0 76-77 28.702979734472606 31.5 27.0 34.0 15.5 36.0 78-79 29.49407038899915 33.0 29.0 35.0 17.0 36.0 80-81 29.549780389453645 33.0 29.0 35.0 17.0 36.0 82-83 29.339174569868852 33.0 29.0 35.0 15.0 35.0 84-85 29.097358676605005 33.0 29.0 35.0 11.0 35.0 86-87 28.811162389342545 33.0 29.0 35.0 7.0 35.0 88-89 28.50804081385308 33.0 28.0 35.0 4.5 35.0 90-91 28.188678852242944 33.0 27.0 35.0 2.0 35.0 92-93 27.826311035193235 33.0 27.0 35.0 2.0 35.0 94-95 27.3933610576656 33.0 26.5 35.0 2.0 35.0 96-97 26.890615452401512 33.0 25.0 35.0 2.0 35.0 98-99 26.351502492159923 32.0 25.0 34.0 2.0 35.0 100-101 25.053501270067315 31.0 21.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5384919394902319 1101 2 0.6074725809581807 1101 3 0.5359654161475866 1101 4 0.4556041091922651 1101 5 0.6190713796437421 1101 6 0.5147768056806896 1101 7 0.5274787931197977 1101 8 0.5387492301686976 1101 9 0.5780088593600823 1101 10-11 0.6135774124790316 1101 12-13 0.6725619460719159 1101 14-15 0.8256338650814357 1101 16-17 0.7954070523478407 1101 18-19 0.8052927600679141 1101 20-21 0.7998454004610664 1101 22-23 0.8017156003234831 1101 24-25 0.7296537231098341 1101 26-27 0.7179074144961319 1101 28-29 0.6888453932866199 1101 30-31 0.6842227137560428 1101 32-33 0.6429702041832641 1101 34-35 0.6613067736182145 1101 36-37 0.8461004743695213 1101 38-39 0.7404339043649628 1101 40-41 0.5403441168017054 1101 42-43 0.5529317104970488 1101 44-45 0.49494588125783423 1101 46-47 0.7694871190827186 1101 48-49 0.7859555672486565 1101 50-51 0.6201994125009875 1101 52-53 0.5044755093190929 1101 54-55 0.6274485380186476 1101 56-57 0.6320354167984021 1101 58-59 0.45625262577922143 1101 60-61 0.5655175599289066 1101 62-63 0.6523252525420844 1101 64-65 0.691388997318839 1101 66-67 0.7409992333004034 1101 68-69 0.6014506549022727 1101 70-71 0.6221605743582437 1101 72-73 0.7035138136091668 1101 74-75 0.898589469735267 1101 76-77 0.6211615706373372 1101 78-79 0.5251548697992448 1101 80-81 0.504113488835209 1101 82-83 0.8351169510227976 1101 84-85 0.8134179180952472 1101 86-87 0.5850733279505356 1101 88-89 0.4299958947461171 1101 90-91 0.511870976342756 1101 92-93 0.7180246905165006 1101 94-95 0.5518080758098094 1101 96-97 0.6617648165811225 1101 98-99 0.5994084514748437 1101 100-101 0.7309966472635985 1102 1 0.5704678628419799 1102 2 0.6273638968895128 1102 3 0.5972907830496652 1102 4 0.41400398286609175 1102 5 0.5814430601785432 1102 6 0.5492053547732638 1102 7 0.49702710704656283 1102 8 0.5133089749027135 1102 9 0.758882812443602 1102 10-11 0.797817600225855 1102 12-13 0.771757550170058 1102 14-15 0.9235243969192837 1102 16-17 0.8816417778878929 1102 18-19 0.9548121138753132 1102 20-21 0.9710757038072373 1102 22-23 0.93868401333539 1102 24-25 0.9927326435188988 1102 26-27 1.0294515574811811 1102 28-29 1.0554592684741735 1102 30-31 0.9734260265510031 1102 32-33 1.1332966064310028 1102 34-35 1.1382627484805496 1102 36-37 1.2155222676566524 1102 38-39 1.2210378974854805 1102 40-41 1.1593485825563405 1102 42-43 1.0377609017995297 1102 44-45 1.215515928108978 1102 46-47 1.3825671285105088 1102 48-49 1.3045686474525482 1102 50-51 1.1610206959717928 1102 52-53 1.192901807254394 1102 54-55 1.17521173245283 1102 56-57 0.9994683896406116 1102 58-59 1.050850626185266 1102 60-61 1.1200143904954842 1102 62-63 0.8684633821213126 1102 64-65 0.8800362104810304 1102 66-67 0.8740322048980183 1102 68-69 0.744791772126586 1102 70-71 0.8757098030607295 1102 72-73 0.7562740482460306 1102 74-75 0.9283972734878709 1102 76-77 1.0303323528872639 1102 78-79 1.2039808842785256 1102 80-81 1.0701313596324695 1102 82-83 1.1658019593576796 1102 84-85 1.21461630541409 1102 86-87 1.364999173554132 1102 88-89 1.4016261791122417 1102 90-91 1.2937407203689055 1102 92-93 1.3546776770336706 1102 94-95 1.187152489649371 1102 96-97 1.1806359553291053 1102 98-99 1.1774838986920528 1102 100-101 1.318304056246916 1103 1 0.4153709369502181 1103 2 0.5840738601453737 1103 3 0.47451546664181876 1103 4 0.30490943097823475 1103 5 0.4204530854306867 1103 6 0.46309289956936084 1103 7 0.5884077339650418 1103 8 0.43760622438481533 1103 9 0.5752003090035629 1103 10-11 0.5228121188474262 1103 12-13 0.5950303489240838 1103 14-15 0.6810183863043164 1103 16-17 0.6604405621559692 1103 18-19 0.6940455525519198 1103 20-21 0.7070002800399777 1103 22-23 0.8927869196000415 1103 24-25 0.7787689264638686 1103 26-27 0.9075400307093702 1103 28-29 0.8352997036578245 1103 30-31 0.9856927844479699 1103 32-33 0.8981607816536652 1103 34-35 0.8973041877015149 1103 36-37 0.8939938703360966 1103 38-39 0.9541006271363344 1103 40-41 0.9613933257254104 1103 42-43 1.0501671513269955 1103 44-45 1.0342544928194783 1103 46-47 0.8652448385796063 1103 48-49 1.0013455969331417 1103 50-51 0.8278328396821948 1103 52-53 0.694345677939225 1103 54-55 0.849221115060601 1103 56-57 0.9111526093191387 1103 58-59 0.8823271583250545 1103 60-61 0.8361547818280997 1103 62-63 0.7844663609669738 1103 64-65 0.5708577367992049 1103 66-67 0.5387296706110511 1103 68-69 0.5930464256278576 1103 70-71 0.49564727754397 1103 72-73 0.7395280591029412 1103 74-75 0.887635664306373 1103 76-77 0.758024654763787 1103 78-79 0.8330025149319091 1103 80-81 0.6318860811784432 1103 82-83 0.7933379474422964 1103 84-85 0.8514993668206507 1103 86-87 0.9993364181974975 1103 88-89 0.9766430012736471 1103 90-91 0.990037576703866 1103 92-93 1.0585053802983069 1103 94-95 1.2789192702279024 1103 96-97 1.2324548279040677 1103 98-99 1.2215214820468034 1103 100-101 1.3672622163182844 1104 1 0.41685030918691623 1104 2 0.49109227726832216 1104 3 0.3513784069398902 1104 4 0.2572635433548882 1104 5 0.29547870543478894 1104 6 0.32182785696694793 1104 7 0.32126355922197547 1104 8 0.42816195887373 1104 9 0.3943662680701152 1104 10-11 0.47676748148028025 1104 12-13 0.47363544950089675 1104 14-15 0.46238166332243935 1104 16-17 0.47977414924933726 1104 18-19 0.5582017028169091 1104 20-21 0.5006889750301653 1104 22-23 0.5652040571867829 1104 24-25 0.37113453124236884 1104 26-27 0.6300351263085915 1104 28-29 0.5606331297111709 1104 30-31 0.6652966991845943 1104 32-33 0.5823316451209948 1104 34-35 0.5964100495584503 1104 36-37 0.6660969798725986 1104 38-39 0.5863909823027882 1104 40-41 0.5715630033525585 1104 42-43 0.48460299883673486 1104 44-45 0.5871813952877858 1104 46-47 0.48840605428539874 1104 48-49 0.48208081785077894 1104 50-51 0.5505500973169504 1104 52-53 0.5001024928603144 1104 54-55 0.5743913050878362 1104 56-57 0.578446478086164 1104 58-59 0.5308635240908686 1104 60-61 0.5005982421390556 1104 62-63 0.567720394287619 1104 64-65 0.7173646322403116 1104 66-67 0.6287675150171061 1104 68-69 0.6126676887672637 1104 70-71 0.5450990094562478 1104 72-73 0.26667684544674586 1104 74-75 0.604401028988832 1104 76-77 0.8619986833996336 1104 78-79 0.732415816838035 1104 80-81 0.6230574277658576 1104 82-83 0.648582362281406 1104 84-85 0.818933047610038 1104 86-87 0.5365476441512946 1104 88-89 0.722731558647812 1104 90-91 0.6585893876721549 1104 92-93 0.6918615276352895 1104 94-95 0.511634917462402 1104 96-97 0.4384634508654486 1104 98-99 0.5054983654831062 1104 100-101 0.6484015013005724 1105 1 0.16982651443570163 1105 2 0.26881447069383313 1105 3 0.2777151554609141 1105 4 0.15644660701153867 1105 5 0.13345713366372536 1105 6 0.2514880849092833 1105 7 0.23302365605456288 1105 8 0.27880960127387056 1105 9 0.23996735552223214 1105 10-11 0.24177596567727022 1105 12-13 0.2135744256820331 1105 14-15 0.39489258854309384 1105 16-17 0.3492841505327817 1105 18-19 0.34559173266227816 1105 20-21 0.35043306640564253 1105 22-23 0.3746031860054728 1105 24-25 0.4900847102507697 1105 26-27 0.3777311332405944 1105 28-29 0.5563795085935013 1105 30-31 0.5193646110510528 1105 32-33 0.5216425141821475 1105 34-35 0.5210751311847233 1105 36-37 0.4673538677429292 1105 38-39 0.46167647748136886 1105 40-41 0.48486695458456097 1105 42-43 0.3613197812378246 1105 44-45 0.6115146962395883 1105 46-47 0.549382896292137 1105 48-49 0.490008383442877 1105 50-51 0.6784618645046336 1105 52-53 0.6531287949346023 1105 54-55 0.5434388768631493 1105 56-57 0.7261308914853046 1105 58-59 0.4710059720919233 1105 60-61 0.48063042039724735 1105 62-63 0.22830216982121954 1105 64-65 0.36464132200658383 1105 66-67 0.40031269540468273 1105 68-69 0.3009163491292206 1105 70-71 0.3410966170048759 1105 72-73 0.4458001190917038 1105 74-75 0.282427526061376 1105 76-77 0.528248171972379 1105 78-79 0.5054977607792246 1105 80-81 0.6087920642075595 1105 82-83 0.6362515587254869 1105 84-85 0.6571915642476434 1105 86-87 0.5269087029280257 1105 88-89 0.5043959077897142 1105 90-91 0.3937198840526328 1105 92-93 0.5265771031744251 1105 94-95 0.3709004192921981 1105 96-97 0.37586546424020284 1105 98-99 0.4309946715069941 1105 100-101 0.7425413427067156 1106 1 0.18646966772169904 1106 2 0.1813482357668299 1106 3 0.18918763134907834 1106 4 0.07975297078053956 1106 5 0.03847086632791985 1106 6 0.19483677276348743 1106 7 0.14047973490087884 1106 8 0.13096178383968748 1106 9 0.32682486301889213 1106 10-11 0.21558351148941313 1106 12-13 0.2695638928206705 1106 14-15 0.3440426010788329 1106 16-17 0.2179316424624531 1106 18-19 0.2183758444159949 1106 20-21 0.33068572193036516 1106 22-23 0.3377443682752457 1106 24-25 0.4435602641254519 1106 26-27 0.5343605255330246 1106 28-29 0.36728996510719725 1106 30-31 0.21761870712446552 1106 32-33 0.44169985283573965 1106 34-35 0.3109476631650452 1106 36-37 0.36787013517788836 1106 38-39 0.23195712242790734 1106 40-41 0.2214974375579004 1106 42-43 0.35247676704905473 1106 44-45 0.19586983469232422 1106 46-47 -0.002302099479699393 1106 48-49 0.19882614907679397 1106 50-51 0.003845444232695172 1106 52-53 -0.09776039330134267 1106 54-55 0.14641579833836715 1106 56-57 0.2408179014756584 1106 58-59 0.3336950883712575 1106 60-61 0.12800496022197194 1106 62-63 0.10858170283981394 1106 64-65 0.013909149327723469 1106 66-67 -0.11815823369964207 1106 68-69 -0.058464073290078744 1106 70-71 -0.1263361113546715 1106 72-73 -0.05258971009633484 1106 74-75 0.08300254153629538 1106 76-77 0.06306939945583778 1106 78-79 0.023231063800128737 1106 80-81 -0.06210116804656707 1106 82-83 -0.010262830471955198 1106 84-85 0.2962477144839859 1106 86-87 0.25046234672297274 1106 88-89 0.3393652624890642 1106 90-91 0.29216368069871734 1106 92-93 0.3420046784207855 1106 94-95 0.4080172113871683 1106 96-97 0.2651030995306698 1106 98-99 0.4181400408582938 1106 100-101 0.6394810266358881 1107 1 0.08704340079964012 1107 2 0.13092164202169698 1107 3 0.2041680564397126 1107 4 -0.07033776743969611 1107 5 0.15278102831909024 1107 6 0.10045282046586124 1107 7 0.050429291069633564 1107 8 0.1230204658187759 1107 9 -0.0320603117625069 1107 10-11 0.16572523824601149 1107 12-13 0.1330463098772512 1107 14-15 0.0355738499153091 1107 16-17 0.102117163510826 1107 18-19 0.10763936172175903 1107 20-21 0.17154115719112895 1107 22-23 0.016353694730128154 1107 24-25 0.11634641703176385 1107 26-27 0.1331340081845056 1107 28-29 0.1517092956577173 1107 30-31 0.008723086415734826 1107 32-33 0.15596892061773815 1107 34-35 -0.07122710305992541 1107 36-37 0.06480499671936002 1107 38-39 0.23064500363530271 1107 40-41 0.06426468436998789 1107 42-43 0.19759365801012052 1107 44-45 0.33957907661448417 1107 46-47 0.13199787859419843 1107 48-49 0.19780206897342367 1107 50-51 0.013906802851373357 1107 52-53 0.016296722133390062 1107 54-55 0.06993195953247522 1107 56-57 0.13465521805137826 1107 58-59 0.017920048828422352 1107 60-61 -0.03647719758254908 1107 62-63 -0.06829559922881856 1107 64-65 -0.05177091156892999 1107 66-67 -0.06693888715273033 1107 68-69 -0.01619599011429429 1107 70-71 -0.00884752602971517 1107 72-73 0.041785278699290274 1107 74-75 0.1285340289398178 1107 76-77 0.12527489670266334 1107 78-79 0.12957732664034793 1107 80-81 0.09052400036384967 1107 82-83 0.12396302633540657 1107 84-85 0.115581436695404 1107 86-87 0.09250217087285151 1107 88-89 0.2261203782354535 1107 90-91 0.2948861720558362 1107 92-93 0.3101854529500976 1107 94-95 0.2767567223660805 1107 96-97 0.6073680454807544 1107 98-99 0.4761426899178858 1107 100-101 0.8103645038679019 1108 1 0.1551145446854889 1108 2 0.4430426776816532 1108 3 0.31384578744790304 1108 4 0.10930332359379946 1108 5 0.21158530918100382 1108 6 0.1926215139003773 1108 7 0.24548272905331459 1108 8 0.3825485466503835 1108 9 0.2852026947006081 1108 10-11 0.42378990379788206 1108 12-13 0.3616740941877694 1108 14-15 0.4658200927793743 1108 16-17 0.46934903559052543 1108 18-19 0.45126088276045806 1108 20-21 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-0.8250765253756072 2316 62-63 -1.034912046248408 2316 64-65 -0.8888075016850294 2316 66-67 -0.5960970719660104 2316 68-69 -0.5464576838698321 2316 70-71 -0.5206502567574169 2316 72-73 -0.5344397734496162 2316 74-75 -0.15666751326060435 2316 76-77 -0.7196310976994127 2316 78-79 -0.81088762894219 2316 80-81 -0.6818157176645627 2316 82-83 -0.8970028574524243 2316 84-85 -0.8234645812979835 2316 86-87 -0.78603402009335 2316 88-89 -1.1318954596236104 2316 90-91 -0.8503955460142372 2316 92-93 -0.86814237823328 2316 94-95 -0.6598039930300246 2316 96-97 -0.5765712711573308 2316 98-99 -0.5372637154258122 2316 100-101 -0.8003311017885117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 20.0 4 59.0 5 88.0 6 198.0 7 485.0 8 1060.0 9 1623.0 10 2353.0 11 3239.0 12 4411.0 13 5547.0 14 6513.0 15 7836.0 16 9264.0 17 10585.0 18 10504.0 19 10366.0 20 10829.0 21 11648.0 22 13063.0 23 15233.0 24 18532.0 25 23178.0 26 29166.0 27 36854.0 28 45947.0 29 55694.0 30 66723.0 31 78661.0 32 93747.0 33 112298.0 34 138475.0 35 172546.0 36 220929.0 37 244581.0 38 115481.0 39 6425.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.357330551974336 17.009201466274547 12.316984010132094 26.316483971619025 2 18.05800960408859 19.137790958622375 35.59553997589824 27.208659461390795 3 21.27640367727685 22.492925035998198 26.825271272710744 29.405400014014205 4 15.24171385848013 14.907161368217386 32.524399977772276 37.32672479553021 5 16.014337089517802 36.023704930678605 31.771058547113483 16.190899432690102 6 36.61057413102651 32.100635791153465 15.723332373839138 15.565457703980892 7 31.826674948616564 29.44378374010837 18.885496992743196 19.84404431853187 8 29.25137990415158 32.56112988016422 18.113482913428374 20.074007302255822 9 28.200607511324556 14.030835113447562 17.446823821715878 40.321733553512004 10-11 28.2351998020412 23.719611808848555 25.075244544770502 22.969943844339742 12-13 28.199124082799297 20.791639523080885 25.468794976290393 25.540441417829424 14-15 25.31051798499843 22.126732179520157 22.694096426040808 29.868653409440604 16-17 25.867269128021775 24.932361971710073 23.395624706470525 25.804744193797628 18-19 25.858368556870232 24.32349346786452 24.72648102978977 25.09165694547548 20-21 26.71882022613763 23.451553118103636 24.487712161841422 25.34191449391731 22-23 26.465698860758096 23.216200307101364 24.443280145565417 25.874820686575124 24-25 26.213086927401953 23.426943347298664 24.476615697520643 25.883354027778744 26-27 26.084973550317525 23.681303420152258 24.25603166386809 25.977691365662125 28-29 25.91095954184138 23.92045033116763 24.361312803096894 25.807277323894095 30-31 26.25442688770067 23.609815222812124 24.404508639138424 25.731249250348785 32-33 26.07573293732761 23.639432749584785 24.20401871303823 26.080815600049373 34-35 26.117455061420138 23.797615797041292 24.47906917282078 25.60585996871779 36-37 26.073147260015634 23.63538921719106 24.390868223263993 25.90059529952931 38-39 26.18327694172306 23.574512675487323 24.339397535602465 25.90281284718715 40-41 26.165627877160762 23.396136252233475 24.560462157435396 25.87777371317037 42-43 26.112378617179637 23.43327622190882 24.3390310080945 26.115314152817042 44-45 25.870833642405426 23.4800202646078 24.460928289487182 26.18821780349959 46-47 26.088553967012423 23.495139992336107 24.292589064367245 26.12371697628422 48-49 26.140373138992473 23.64192141392065 24.129193994271542 26.088511452815332 50-51 25.936171787009098 23.546344890650584 24.368449643238158 26.149033679102164 52-53 26.070313286995063 23.466572436466457 24.28985512738287 26.17325914915561 54-55 25.739355910790835 23.35460262372107 24.439364844461014 26.46667662102708 56-57 25.961417358874115 23.487084142859622 24.133926556302786 26.417571941963473 58-59 25.914028189217785 23.601461737973114 24.189294814971955 26.295215257837146 60-61 26.00574181778334 23.635181407904334 24.27631211210282 26.082764662209513 62-63 25.83361214923301 23.69543383280082 24.014468728545786 26.456485289420385 64-65 25.66464118980757 23.577131995541254 24.19956170837683 26.558665106274344 66-67 25.77458059941723 23.722271407961266 24.05830374289782 26.444844249723687 68-69 25.748738923618188 23.6282664366476 24.146883604980165 26.476111034754048 70-71 25.581138199669727 23.37834332765614 24.266127317594528 26.774391155079602 72-73 25.671566174947063 23.66078422454353 24.03636476194808 26.63128483856133 74-75 25.573572182816783 23.624568749260554 24.106038491006764 26.695820576915896 76-77 25.59251260537179 23.44782711620235 23.903462410483822 27.056197867942032 78-79 25.562297375824585 23.499780153460144 23.954182276612716 26.983740194102555 80-81 25.52550097686148 23.69420921563862 23.94917164040135 26.83111816709855 82-83 25.741051454138702 23.487565712727438 24.098801389751593 26.672581443382263 84-85 25.525133697941126 23.461368996217573 23.863662187343767 27.149835118497535 86-87 25.14382944233187 23.719801182702845 24.06727064301261 27.069098731952675 88-89 25.313571936274354 23.348184470566846 24.033813375618465 27.304430217540332 90-91 25.358782958207982 23.41540354422357 23.988547226906885 27.237266270661564 92-93 25.460968056594663 23.32821935797512 24.082656994308334 27.12815559112189 94-95 25.218154576566914 23.20053050849972 24.280990227823857 27.300324687109505 96-97 25.058421834047905 23.188449709879254 24.24433519677206 27.50879325930078 98-99 24.863871761075846 23.066713883881 24.25639199882841 27.813022356214745 100-101 24.94886905934219 23.332689462228753 23.997959812343137 27.720481666085924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 100.0 1 77.0 2 60.0 3 67.0 4 58.5 5 73.0 6 111.0 7 168.0 8 258.0 9 323.0 10 316.5 11 269.0 12 277.0 13 312.0 14 368.5 15 410.0 16 452.5 17 523.0 18 576.5 19 646.5 20 688.5 21 735.5 22 870.5 23 1010.0 24 1134.5 25 1298.5 26 1469.5 27 1678.0 28 1935.0 29 2282.5 30 2757.0 31 3231.0 32 3905.5 33 4491.0 34 4980.0 35 5758.5 36 6830.5 37 7873.5 38 8848.0 39 10226.5 40 11867.5 41 14580.5 42 18572.5 43 24041.5 44 31219.0 45 39417.0 46 48420.0 47 57333.0 48 66485.5 49 74456.5 50 79726.5 51 84991.5 52 90825.0 53 95095.5 54 96520.5 55 93601.5 56 87953.0 57 80259.0 58 72541.5 59 65510.0 60 56872.5 61 47598.0 62 38830.5 63 30626.5 64 23368.5 65 17660.5 66 13191.5 67 10352.5 68 8087.0 69 6106.0 70 4818.5 71 3797.5 72 2997.5 73 2477.5 74 1931.0 75 1476.5 76 1084.0 77 703.0 78 463.5 79 319.0 80 225.0 81 137.5 82 85.0 83 57.5 84 31.0 85 22.0 86 12.5 87 7.0 88 4.5 89 2.0 90 2.0 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01830613924785755 2 0.0032193555228990866 3 0.003850601703859692 4 0.03534978613379389 5 0.001578115452401513 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.156230904803026E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.156230904803026E-5 24-25 4.418723266724236E-4 26-27 0.0017674893066896944 28-29 3.156230904803026E-5 30-31 0.006186212573413931 32-33 0.022251427878861335 34-35 0.012656485928260134 36-37 4.734346357204539E-4 38-39 5.049969447684841E-4 40-41 0.002051550088121967 42-43 0.008206200352487867 44-45 0.007922139571055595 46-47 0.007385580317239081 48-49 0.009279318860120896 50-51 0.18059953237282914 52-53 0.051225627584953116 54-55 0.017201458431176493 56-57 0.010447124294898016 58-59 0.018937385428818156 60-61 0.055013104670716746 62-63 0.09989470813701576 64-65 0.10478686603946047 66-67 0.10926871392428075 68-69 0.1067121668913903 70-71 0.09591785719696395 72-73 0.07271956004666172 74-75 0.2266805035829533 76-77 0.2705521131597154 78-79 0.006849021063422567 80-81 8.206200352487868E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.2624923619212103E-4 90-91 0.0061546502643659 92-93 0.017233020740224522 94-95 0.0017359269976416644 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1584168.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.06313594285016 #Duplication Level Percentage of deduplicated Percentage of total 1 76.29947683447284 42.01288465304925 2 12.619870970329117 13.897793416409213 3 4.340438993226129 7.169945470069742 4 2.0979446717818204 4.620776506516022 5 1.1500109101903788 3.1661603541786834 6 0.7401094706694162 2.4451649037636565 7 0.49334027022974886 1.901540365602017 8 0.3703557520489466 1.631435929783012 9 0.27912434948376214 1.3832515800525613 >10 1.4778401668215917 14.602575516322675 >50 0.09682435621892069 3.61401632369946 >100 0.03351173454359186 2.9813888284045533 >500 8.060639886819464E-4 0.30270168293427296 >1k 3.4545599514940563E-4 0.2703644692148596 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8937385428818157E-4 2 0.0 0.0 0.0 0.0 1.8937385428818157E-4 3 0.0 0.0 0.0 0.0 1.8937385428818157E-4 4 0.0 0.0 0.0 0.0 1.8937385428818157E-4 5 0.0 0.0 0.0 0.0 1.8937385428818157E-4 6 0.0 0.0 0.0 0.0 3.1562309048030257E-4 7 0.0 0.0 0.0 0.0 3.1562309048030257E-4 8 0.0 0.0 0.0 0.0 3.1562309048030257E-4 9 0.0 0.0 0.0 0.0 3.1562309048030257E-4 10-11 0.0 0.0 0.0 0.0 3.1562309048030257E-4 12-13 0.0 0.0 0.0 0.0 0.0013256169800172708 14-15 0.0 0.0 0.0 0.0 0.001704364688593634 16-17 0.0 0.0 0.0 3.156230904803026E-5 0.002114674706218027 18-19 0.0 0.0 0.0 9.468692714409077E-5 0.002335610869554239 20-21 0.0 0.0 0.0 1.578115452401513E-4 0.0026196716509865117 22-23 0.0 0.0 0.0 5.365592538165144E-4 0.0028721701233707536 24-25 0.0 0.0 0.0 0.00123093005287318 0.002903732432418784 26-27 0.0 0.0 0.0 0.0023356108695542395 0.003029981668610905 28-29 0.0 0.0 0.0 0.004671221739108479 0.003029981668610905 30-31 0.0 0.0 0.0 0.01675958610450407 0.003124668595754996 32-33 0.0 0.0 0.0 0.04901626595159099 0.0032193555228990866 34-35 0.0 0.0 0.0 0.09203569318405624 0.0033456047590912077 36-37 0.0 0.0 0.0 0.1628299523787881 0.0033771670681392377 38-39 0.0 0.0 0.0 0.26496558445821405 0.003534978613379389 40-41 0.0 0.0 0.0 0.37499179379964753 0.003566540922427419 42-43 0.0 0.0 0.0 0.4851442523772731 0.0035981032314754496 44-45 0.0 0.0 0.0 0.6171693911251837 0.0036927901586195403 46-47 0.0 0.0 0.0 0.7658278667414062 0.003787477085763631 48-49 0.0 0.0 0.0 0.9198203725867459 0.004008413249099843 50-51 0.0 0.0 0.0 1.0835656319279268 0.004229349412436054 52-53 0.0 0.0 0.0 1.2410931163866459 0.004481847884820297 54-55 0.0 0.0 0.0 1.4126342660626903 0.004734346357204539 56-57 0.0 0.0 0.0 1.599262199463693 0.0048605955933966605 58-59 0.0 0.0 0.0 1.8037228374768333 0.005018407138636811 60-61 0.0 0.0 0.0 2.0227652622701635 0.005176218683876963 62-63 0.0 0.0 0.0 2.2427545563349343 0.005302467920069083 64-65 0.0 0.0 0.0 2.461891668055408 0.005365592538165144 66-67 0.0 0.0 0.0 2.702743648400927 0.005460279465309235 68-69 0.0 0.0 0.0 2.974589816231612 0.005554966392453326 70-71 0.0 0.0 0.0 3.2768936122936454 0.005554966392453326 72-73 6.312461809606051E-5 0.0 0.0 3.5822589523333384 0.005870589482933628 74-75 6.312461809606051E-5 0.0 0.0 3.8819115144353376 0.006280899500558022 76-77 6.312461809606051E-5 0.0 0.0 4.216661364198746 0.006375586427702112 78-79 6.312461809606051E-5 0.0 0.0 4.593957206558901 0.006501835663894233 80-81 6.312461809606051E-5 0.0 0.0 4.999438190898945 0.006628084900086354 82-83 6.312461809606051E-5 0.0 0.0 5.423256876795895 0.007669641098671354 84-85 6.312461809606051E-5 0.0 0.0 5.851588972886714 0.007701203407719384 86-87 6.312461809606051E-5 0.0 0.0 6.291409749471016 0.008174638043439838 88-89 6.312461809606051E-5 0.0 0.0 6.772892773998717 0.008679634988208323 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1850 0.0 52.86243 1 GTATCAA 3125 0.0 42.080547 1 GTGGTAT 825 0.0 30.498154 1 CAACGCA 4195 0.0 30.430382 5 TCAACGC 4305 0.0 29.879904 4 ATCAACG 4395 0.0 29.268953 3 TATCAAC 4485 0.0 29.10496 2 AACGCAG 4485 0.0 28.780182 6 TGGTATC 835 0.0 27.286734 2 GTACATG 7860 0.0 26.454721 1 GTACTAG 315 0.0 25.620642 1 TATTACG 95 0.0012334713 24.976612 5 TACATGG 8200 0.0 24.83363 2 TAGTACT 720 0.0 24.392275 4 ACATGGG 8240 0.0 24.25223 3 CTAGTAC 705 0.0 24.238747 3 TAATACC 235 1.7826096E-10 24.237982 4 ACGCAGA 5355 0.0 23.927174 7 GAGTACA 5800 0.0 22.427155 1 CGCAGAG 5695 0.0 22.415356 8 >>END_MODULE