FastQCFastQC Report
Sat 18 Jun 2016
SRR1930934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989106
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36470.1833487003709204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.057.1719061
GTATCAA29550.043.0036541
ATCAACG36100.035.319193
TCAACGC36550.035.0020684
CAACGCA36550.034.656615
TATCAAC38600.032.5160452
AACGCAG40350.031.6199446
GTACATG45450.030.0675261
TACATGG47650.027.821372
ACGCAGA46050.027.7246257
CGCAGAG50250.026.258358
ACATGGG50450.025.9722483
GCAGAGT52150.023.4819159
CATGGGG37200.020.8509164
CAGAGTA49950.019.55319410-11
GAGTACA33800.019.3949621
AGAGTAC46950.019.24946410-11
GAGTACT28550.018.8699612-13
AGTACAT32900.018.6917342
GTATTAG4650.018.435641
Produced by FastQC (version 0.11.5)