##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930934_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1989106 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.376331377010576 30.0 10.0 31.0 2.0 33.0 2 21.279637686478246 28.0 10.0 31.0 2.0 33.0 3 21.715754715937713 28.0 10.0 31.0 2.0 33.0 4 23.622305196404817 32.0 2.0 35.0 2.0 37.0 5 23.580423567170378 32.0 10.0 35.0 2.0 37.0 6 24.291816524609548 32.0 10.0 35.0 2.0 37.0 7 24.346202766468956 32.0 10.0 35.0 2.0 37.0 8 25.071961976888108 32.0 17.0 35.0 2.0 37.0 9 26.180460970908538 33.0 17.0 37.0 2.0 39.0 10-11 26.372417809810038 33.0 17.0 37.0 2.0 39.0 12-13 26.27393311367016 33.0 16.5 37.0 2.0 39.0 14-15 26.443660870763047 33.0 13.5 38.0 2.0 40.0 16-17 25.56898651957211 32.0 10.0 38.0 2.0 40.0 18-19 24.943298899103418 32.0 10.0 38.0 2.0 40.0 20-21 24.408563193716173 31.5 6.5 38.0 2.0 40.0 22-23 24.302490415292098 31.0 8.0 37.0 2.0 39.5 24-25 23.9849392138981 31.0 5.0 37.0 2.0 39.0 26-27 23.615095676147977 30.0 2.0 37.0 2.0 39.0 28-29 23.4950450101704 30.0 2.0 37.0 2.0 39.0 30-31 23.38363440661282 30.0 2.0 37.0 2.0 39.0 32-33 23.034013270283232 30.0 2.0 37.0 2.0 39.0 34-35 20.25141520864147 24.0 2.0 35.0 2.0 39.0 36-37 18.64666412951346 17.0 2.0 34.0 2.0 38.0 38-39 17.73764947669958 15.5 2.0 33.0 2.0 38.0 40-41 18.927197193110874 19.5 2.0 34.0 2.0 38.0 42-43 18.81482736465528 18.5 2.0 34.0 2.0 38.0 44-45 16.93075507288199 12.0 2.0 32.5 2.0 38.0 46-47 17.648535824636795 17.0 2.0 32.0 2.0 38.0 48-49 17.07758611154961 13.0 2.0 33.0 2.0 38.0 50-51 15.245381342170806 9.5 2.0 30.5 2.0 37.0 52-53 17.960535537070424 18.5 2.0 32.0 2.0 37.5 54-55 19.42264992413677 24.0 2.0 34.5 2.0 38.0 56-57 19.585519826494917 24.5 2.0 35.0 2.0 39.0 58-59 19.207572145476412 23.0 2.0 35.0 2.0 38.5 60-61 18.868726704358643 22.5 2.0 34.0 2.0 38.0 62-63 18.386342155722218 21.0 2.0 34.0 2.0 38.0 64-65 17.875824616687094 19.0 2.0 34.0 2.0 38.0 66-67 17.45160388636905 18.0 2.0 33.0 2.0 37.0 68-69 16.987678635527722 13.5 2.0 32.5 2.0 36.5 70-71 16.414514611086588 8.0 2.0 32.0 2.0 36.0 72-73 15.934161879759046 7.0 2.0 31.5 2.0 35.5 74-75 15.394104436867618 2.0 2.0 31.0 2.0 35.0 76-77 14.831613297632202 2.0 2.0 31.0 2.0 34.5 78-79 14.33982527829085 2.0 2.0 30.0 2.0 34.0 80-81 13.834126989712967 2.0 2.0 30.0 2.0 34.0 82-83 13.36682559903796 2.0 2.0 29.0 2.0 34.0 84-85 12.855436060220018 2.0 2.0 29.0 2.0 34.0 86-87 12.312664332619779 2.0 2.0 28.5 2.0 34.0 88-89 11.777255711862516 2.0 2.0 27.0 2.0 33.5 90-91 11.221143569020455 2.0 2.0 26.0 2.0 33.0 92-93 10.624508196144397 2.0 2.0 25.0 2.0 32.5 94-95 10.001123368990893 2.0 2.0 23.5 2.0 32.0 96-97 9.313692432680813 2.0 2.0 19.0 2.0 32.0 98-99 8.571876511357363 2.0 2.0 2.0 2.0 32.0 100-101 7.6401174698583185 2.0 2.0 2.0 2.0 30.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.894422251322666 1101 2 7.865051538598529 1101 3 7.391906220850991 1101 4 9.328326112939944 1101 5 9.374401836539633 1101 6 8.733193005419785 1101 7 8.273918938329299 1101 8 7.621841985986485 1101 9 7.778731621879221 1101 10-11 7.05780379255679 1101 12-13 7.109169792261113 1101 14-15 7.675390945979892 1101 16-17 8.155591481984843 1101 18-19 8.63655261878996 1101 20-21 8.749726442683674 1101 22-23 8.729151362523645 1101 24-25 8.846023284162495 1101 26-27 8.886326583029 1101 28-29 8.808558054016736 1101 30-31 8.43652176460828 1101 32-33 8.581918734841164 1101 34-35 10.855257674926506 1101 36-37 5.768294336459107 1101 38-39 8.660093036884255 1101 40-41 9.938920130385792 1101 42-43 10.171136918497243 1101 44-45 4.966978884039037 1101 46-47 6.290186168284411 1101 48-49 9.262609868025631 1101 50-51 -2.9318288437827142 1101 52-53 6.076960624577573 1101 54-55 8.54514330402737 1101 56-57 9.04243693217413 1101 58-59 9.260690531941062 1101 60-61 9.090769808810183 1101 62-63 9.285299188390155 1101 64-65 9.206385828899975 1101 66-67 9.011365091541574 1101 68-69 9.03853018384956 1101 70-71 9.127404078752324 1101 72-73 8.752494317713216 1101 74-75 9.024455944467482 1101 76-77 8.790997172794897 1101 78-79 8.535556440785511 1101 80-81 8.27888376976796 1101 82-83 8.19160610466352 1101 84-85 8.174808833805274 1101 86-87 7.585109628922028 1101 88-89 7.436375589590499 1101 90-91 7.329483684721826 1101 92-93 7.356798444557754 1101 94-95 6.87243957834046 1101 96-97 6.829379297493176 1101 98-99 6.323795670161079 1101 100-101 5.739187434692538 1102 1 6.772717757264033 1102 2 7.8615340023130145 1102 3 7.62174463210053 1102 4 9.38809236432763 1102 5 9.557437073577582 1102 6 8.761827724386933 1102 7 8.448343304103727 1102 8 7.436412213362004 1102 9 7.370224570359955 1102 10-11 7.254007433443263 1102 12-13 6.906942293391822 1102 14-15 7.15750465429781 1102 16-17 8.110017954991712 1102 18-19 8.406502713358439 1102 20-21 8.739991542802912 1102 22-23 8.442466244724216 1102 24-25 8.532795518034458 1102 26-27 8.584023789147171 1102 28-29 8.345965541373339 1102 30-31 8.066357349082601 1102 32-33 8.185040265617037 1102 34-35 10.504373408243392 1102 36-37 1.468487071132845 1102 38-39 4.029127770242258 1102 40-41 8.62923585547933 1102 42-43 9.686446219101658 1102 44-45 1.687084708223896 1102 46-47 2.5739401771656247 1102 48-49 7.74071263929326 1102 50-51 -4.022492005248115 1102 52-53 5.205128925634273 1102 54-55 8.170312910540261 1102 56-57 8.66718894689392 1102 58-59 9.066596693283113 1102 60-61 9.013287129232861 1102 62-63 8.818830139008963 1102 64-65 8.77900696906691 1102 66-67 8.938382124147143 1102 68-69 8.730144169705405 1102 70-71 8.488344983731562 1102 72-73 8.444620720821641 1102 74-75 8.49548406831107 1102 76-77 8.272750595762703 1102 78-79 8.050885528816432 1102 80-81 7.937887819765907 1102 82-83 7.862384444369944 1102 84-85 7.838598357262368 1102 86-87 7.644939097282704 1102 88-89 7.4950668954578585 1102 90-91 7.251670529423933 1102 92-93 6.951275696726661 1102 94-95 6.482603377115181 1102 96-97 6.433685247084064 1102 98-99 6.098288906458928 1102 100-101 5.783389461747347 1103 1 6.707476016624803 1103 2 7.734173726245714 1103 3 7.327748950498794 1103 4 8.896520510978693 1103 5 8.845001201307177 1103 6 8.123893673259232 1103 7 7.91599433293425 1103 8 7.205480171186139 1103 9 7.170422072862056 1103 10-11 6.563041415990341 1103 12-13 6.725213940514223 1103 14-15 7.261943129239228 1103 16-17 7.74153014562161 1103 18-19 7.928005889150203 1103 20-21 8.441164441097605 1103 22-23 8.153275326446632 1103 24-25 8.322282326563656 1103 26-27 8.228255019745273 1103 28-29 8.221283786969522 1103 30-31 8.066746053393487 1103 32-33 7.709096433874624 1103 34-35 10.169768061862776 1103 36-37 -3.0330719869619713 1103 38-39 -1.5651811285177182 1103 40-41 6.544303478882046 1103 42-43 8.565986171555654 1103 44-45 0.9327625537745163 1103 46-47 1.0367892295171153 1103 48-49 7.118627618706956 1103 50-51 -4.4328376225100765 1103 52-53 4.33056009904147 1103 54-55 6.98496529401984 1103 56-57 7.571227570143964 1103 58-59 7.674952139626651 1103 60-61 7.585417708678545 1103 62-63 7.509815543210554 1103 64-65 7.5077069906346665 1103 66-67 7.510391536413625 1103 68-69 7.521161804376664 1103 70-71 7.626722627902328 1103 72-73 7.507242584712126 1103 74-75 7.391569883754071 1103 76-77 7.144270250290839 1103 78-79 7.053538968873815 1103 80-81 6.867106558027837 1103 82-83 6.630334150297202 1103 84-85 6.624153775495122 1103 86-87 6.306269803711592 1103 88-89 6.443609133124857 1103 90-91 6.321520257888288 1103 92-93 5.944186810907343 1103 94-95 5.614920723650892 1103 96-97 5.501063649761374 1103 98-99 5.116946592214271 1103 100-101 4.250400364370132 1104 1 6.40046576421722 1104 2 7.426010415428163 1104 3 6.857673421886538 1104 4 8.626301319551512 1104 5 8.549759797308326 1104 6 7.882084272969323 1104 7 7.591629283843584 1104 8 6.994084094918083 1104 9 6.918908859756346 1104 10-11 6.405546125525028 1104 12-13 6.077839014605033 1104 14-15 6.591797647164796 1104 16-17 7.408562581257968 1104 18-19 7.840416877477605 1104 20-21 7.884693335046514 1104 22-23 7.784947812787507 1104 24-25 8.071619470560979 1104 26-27 8.073728878159937 1104 28-29 7.7930470015423765 1104 30-31 7.48192888897351 1104 32-33 7.347832928807108 1104 34-35 9.635094501571931 1104 36-37 -3.0814266134562587 1104 38-39 -1.7665522633248454 1104 40-41 5.929097990586925 1104 42-43 8.005759292289934 1104 44-45 0.48312259109868094 1104 46-47 0.1429835146948477 1104 48-49 6.298133366980128 1104 50-51 -4.8491221077476645 1104 52-53 3.8149934583921734 1104 54-55 6.3181334464151 1104 56-57 6.649681903796154 1104 58-59 7.066165116228628 1104 60-61 7.120028123058912 1104 62-63 7.222607637060872 1104 64-65 7.006962972183853 1104 66-67 6.819999339276318 1104 68-69 6.706348689276222 1104 70-71 6.78472273353675 1104 72-73 6.780261792571325 1104 74-75 6.817458533335346 1104 76-77 6.57387629257982 1104 78-79 6.255229471752356 1104 80-81 6.105201852014305 1104 82-83 6.03784029763375 1104 84-85 5.725008475346646 1104 86-87 5.440907448298708 1104 88-89 5.503469460943679 1104 90-91 5.406919585349129 1104 92-93 5.471959626475506 1104 94-95 5.073935271858334 1104 96-97 4.621225857286067 1104 98-99 4.3057114323835215 1104 100-101 3.9053033861014788 1105 1 5.6596563846644585 1105 2 6.862584889909581 1105 3 6.681229835641364 1105 4 8.625955830108136 1105 5 8.454441017390188 1105 6 7.533630419166396 1105 7 7.303278799372109 1105 8 6.611559986969976 1105 9 6.646198825762408 1105 10-11 6.091267629583484 1105 12-13 5.932608110181192 1105 14-15 6.188393396765889 1105 16-17 6.816961166000013 1105 18-19 7.448308559368311 1105 20-21 7.7993261079808605 1105 22-23 7.454691586346236 1105 24-25 7.5018891095777 1105 26-27 7.599015051644663 1105 28-29 7.2993824778044285 1105 30-31 6.959045300878326 1105 32-33 6.852575430483679 1105 34-35 8.892419502150059 1105 36-37 -3.4998079699763895 1105 38-39 -2.2607228805345674 1105 40-41 5.120095377447228 1105 42-43 7.078787296822458 1105 44-45 -3.1489878082723166 1105 46-47 -4.050653027635706 1105 48-49 4.515236135058199 1105 50-51 -9.096159894210214 1105 52-53 0.5005386333337789 1105 54-55 4.951420568030162 1105 56-57 5.898244561336103 1105 58-59 6.419156351804325 1105 60-61 6.365011472965254 1105 62-63 6.278722615207624 1105 64-65 6.154772097360183 1105 66-67 6.025092764801833 1105 68-69 6.055223189195285 1105 70-71 6.104602945362945 1105 72-73 6.109667129851351 1105 74-75 5.90499004132508 1105 76-77 5.800905407118593 1105 78-79 5.8472756988873815 1105 80-81 5.752586399802384 1105 82-83 5.370842633271884 1105 84-85 5.2539517725276905 1105 86-87 4.991066664819302 1105 88-89 4.803188953115823 1105 90-91 4.539988058859292 1105 92-93 4.294561666111981 1105 94-95 4.495452280391508 1105 96-97 4.171658528469841 1105 98-99 3.8631252334611528 1105 100-101 3.379874646189818 1106 1 5.781044396024903 1106 2 6.98562177872839 1106 3 6.507948175291091 1106 4 7.736103019268228 1106 5 7.659627255810889 1106 6 7.021437606985273 1106 7 6.818695509084492 1106 8 6.252779385997414 1106 9 6.177743632274183 1106 10-11 5.578553964245348 1106 12-13 5.474705460558816 1106 14-15 5.523685083596078 1106 16-17 6.0948973822181856 1106 18-19 6.6324318495471815 1106 20-21 6.975715895543459 1106 22-23 6.7101434394869415 1106 24-25 6.710262996506117 1106 26-27 6.867661549354633 1106 28-29 6.665475639607383 1106 30-31 6.360790283560355 1106 32-33 6.410574774376354 1106 34-35 8.535205085550906 1106 36-37 -0.3241341006287435 1106 38-39 0.8291031060279188 1106 40-41 5.988701276250829 1106 42-43 6.97505346615252 1106 44-45 -3.3071762814453205 1106 46-47 -4.108546116578122 1106 48-49 4.0398190900423465 1106 50-51 -9.053038282361133 1106 52-53 0.19284332812606664 1106 54-55 4.394679744923582 1106 56-57 5.226520476216148 1106 58-59 5.620920682402669 1106 60-61 5.693679783525138 1106 62-63 5.721772227433693 1106 64-65 5.716698116728036 1106 66-67 5.515195648592904 1106 68-69 5.254735096093292 1106 70-71 5.162128333272808 1106 72-73 5.186130593554298 1106 74-75 5.037864235463976 1106 76-77 4.795208419956184 1106 78-79 4.632311966035436 1106 80-81 4.3391449900053605 1106 82-83 4.213504199700939 1106 84-85 4.077996973247599 1106 86-87 4.0176238322481 1106 88-89 4.026366488145973 1106 90-91 3.515619199670436 1106 92-93 3.291155617237422 1106 94-95 3.0976464337864993 1106 96-97 2.7955712843199816 1106 98-99 2.644184076501068 1106 100-101 2.4522963437678404 1107 1 5.972313248807737 1107 2 7.1487430116911135 1107 3 6.638242361155207 1107 4 8.141663364038028 1107 5 8.347441617822753 1107 6 7.130190830767006 1107 7 7.124413026480958 1107 8 6.31564642564183 1107 9 6.397772349683873 1107 10-11 5.837141926618656 1107 12-13 5.578305661124855 1107 14-15 5.8184953703813775 1107 16-17 6.731216034636084 1107 18-19 7.079001200572154 1107 20-21 7.2337546251667675 1107 22-23 7.376831333619535 1107 24-25 7.284345623635179 1107 26-27 7.410320488329912 1107 28-29 7.456333483960449 1107 30-31 6.965065182372378 1107 32-33 6.693816427401583 1107 34-35 9.113539127211258 1107 36-37 0.17999966905190945 1107 38-39 1.271421154245104 1107 40-41 6.722807043552219 1107 42-43 7.715767902214726 1107 44-45 -2.969555893782635 1107 46-47 -3.733782802823214 1107 48-49 4.6552508097754455 1107 50-51 -8.907591707870827 1107 52-53 0.7926552080885436 1107 54-55 5.146249013273167 1107 56-57 6.396628003386187 1107 58-59 6.794622913342305 1107 60-61 6.528411224798571 1107 62-63 6.425287205310209 1107 64-65 6.293091185938067 1107 66-67 6.37764557970419 1107 68-69 6.304553947602479 1107 70-71 6.208209810096658 1107 72-73 6.092470209770648 1107 74-75 6.033701641119061 1107 76-77 5.874338491588825 1107 78-79 5.6417691372767305 1107 80-81 5.690082596003036 1107 82-83 5.603971704472649 1107 84-85 5.616267748121606 1107 86-87 5.447202272650538 1107 88-89 5.573768983691556 1107 90-91 5.486714429657738 1107 92-93 4.8752567144963095 1107 94-95 4.678882158315664 1107 96-97 4.532087933133232 1107 98-99 4.042563392994541 1107 100-101 3.5257612516384027 1108 1 5.695271654444795 1108 2 6.24225539534784 1108 3 6.405347451337676 1108 4 7.87222724547744 1108 5 8.005944536264149 1108 6 7.535473384972892 1108 7 7.555805990670894 1108 8 6.574057321156367 1108 9 4.748237284566699 1108 10-11 5.76432736158036 1108 12-13 5.435158849500169 1108 14-15 6.071117147387909 1108 16-17 6.0558478333775625 1108 18-19 6.744168550837706 1108 20-21 7.754243201177708 1108 22-23 7.931265304978496 1108 24-25 7.123778105099131 1108 26-27 7.315225419882385 1108 28-29 7.193858023407614 1108 30-31 7.118986564118533 1108 32-33 7.172374428137761 1108 34-35 9.244202042848148 1108 36-37 -3.4442133158212744 1108 38-39 -2.158161477961169 1108 40-41 5.283613273942571 1108 42-43 7.67135500848806 1108 44-45 -2.7869409965853897 1108 46-47 -3.871022509401538 1108 48-49 4.613929886391105 1108 50-51 -9.131260661964532 1108 52-53 0.6220650782047557 1108 54-55 5.048405088539152 1108 56-57 6.189773938602048 1108 58-59 6.73102455397963 1108 60-61 6.71533936803883 1108 62-63 6.752916650893983 1108 64-65 6.712616698199028 1108 66-67 6.715845501703804 1108 68-69 6.7658717816457 1108 70-71 6.422124005290431 1108 72-73 6.249184706449059 1108 74-75 6.134476605624505 1108 76-77 6.260627185038546 1108 78-79 6.101220658722456 1108 80-81 6.120181213906315 1108 82-83 6.017445942435174 1108 84-85 6.037174918898634 1108 86-87 5.901386029289336 1108 88-89 5.568377426137792 1108 90-91 5.839108340369208 1108 92-93 5.8041985822988025 1108 94-95 5.405855173687874 1108 96-97 5.045487347692136 1108 98-99 4.9201034448779435 1108 100-101 4.329868870260337 1109 1 4.842157761692519 1109 2 6.254390304944504 1109 3 6.018643309783048 1109 4 7.714127003371633 1109 5 7.382509209421311 1109 6 5.023660424585525 1109 7 4.5883511923398785 1109 8 3.1662996330092525 1109 9 2.73955109489998 1109 10-11 2.5132356318116464 1109 12-13 3.3137521246250827 1109 14-15 3.9239232566961064 1109 16-17 3.1260414536320305 1109 18-19 3.818621881080329 1109 20-21 4.361603925949765 1109 22-23 6.154348419386366 1109 24-25 6.050881721232649 1109 26-27 4.669572056157044 1109 28-29 4.829989095369704 1109 30-31 4.549897229651936 1109 32-33 4.142091241613269 1109 34-35 6.262857067831401 1109 36-37 -4.454004435174285 1109 38-39 -3.023664942134662 1109 40-41 3.12477415840144 1109 42-43 4.016633945282987 1109 44-45 -4.245542835559274 1109 46-47 -5.414306004997275 1109 48-49 2.035564873255577 1109 50-51 -9.277175126498609 1109 52-53 -0.766887556627573 1109 54-55 2.6866066158662036 1109 56-57 3.81701180755584 1109 58-59 4.6584468998303485 1109 60-61 5.105667369893258 1109 62-63 5.146337940751295 1109 64-65 4.677122441772919 1109 66-67 4.871485729901401 1109 68-69 4.882234206309583 1109 70-71 5.07822140136523 1109 72-73 5.016336550574133 1109 74-75 5.091506044660202 1109 76-77 4.98015308263294 1109 78-79 4.772799948373722 1109 80-81 4.549092574850013 1109 82-83 4.527852306727144 1109 84-85 4.5058801679585425 1109 86-87 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80-81 -11.583833716095455 2313 82-83 -11.100455661321693 2313 84-85 -10.584466938269541 2313 86-87 -10.009545655984454 2313 88-89 -9.502578847121114 2313 90-91 -8.959894849423456 2313 92-93 -8.35786282120997 2313 94-95 -7.9325707522380515 2313 96-97 -7.21354153765254 2313 98-99 -6.39967105450182 2313 100-101 -5.466461760609768 2314 1 -20.249172789999648 2314 2 -19.149411271318826 2314 3 -19.554374770884547 2314 4 -21.453698680448486 2314 5 -21.41072213040252 2314 6 -22.137674763175255 2314 7 -22.17752734266244 2314 8 -22.91483156773252 2314 9 -24.00639234506293 2314 10-11 -24.200718934715937 2314 12-13 -24.10673929864798 2314 14-15 -24.263142111871346 2314 16-17 -23.4133651295854 2314 18-19 -22.78777589360674 2314 20-21 -22.27145124326674 2314 22-23 -22.13216062094743 2314 24-25 -21.797055228234203 2314 26-27 -21.4600060615991 2314 28-29 -21.351049383999793 2314 30-31 -21.205781954399985 2314 32-33 -20.88734779408446 2314 34-35 -18.168760920114817 2314 36-37 -16.571065167673126 2314 38-39 -15.694504070553762 2314 40-41 -16.844395985316687 2314 42-43 -16.750867213734164 2314 44-45 -14.885552107696501 2314 46-47 -15.58352250940154 2314 48-49 -15.04162566916445 2314 50-51 -13.218760661964533 2314 52-53 -15.887657144017467 2314 54-55 -17.318493059608993 2314 56-57 -17.4609205058424 2314 58-59 -17.085642112687037 2314 60-61 -16.763827298627834 2314 62-63 -16.28365742318009 2314 64-65 -15.767012931430603 2314 66-67 -15.356145239036938 2314 68-69 -14.898711551687633 2314 70-71 -14.311903772487344 2314 72-73 -13.832991219476865 2314 74-75 -13.303023394375495 2314 76-77 -12.745159851998492 2314 78-79 -12.264288600536803 2314 80-81 -11.767087304612202 2314 82-83 -11.298063316824084 2314 84-85 -10.795232488508775 2314 86-87 -10.222225081821774 2314 88-89 -9.705928129417766 2314 90-91 -9.169224992964125 2314 92-93 -8.549968084367865 2314 94-95 -7.9325707522380515 2314 96-97 -7.256364504159716 2314 98-99 -6.510915073640575 2314 100-101 -5.593973722332255 2315 1 -20.249172789999648 2315 2 -19.149411271318826 2315 3 -19.554374770884547 2315 4 -16.718708061311524 2315 5 -21.41072213040252 2315 6 -22.137674763175255 2315 7 -22.17752734266244 2315 8 -22.91483156773252 2315 9 -19.5865987240498 2315 10-11 -17.89630992908742 2315 12-13 -14.75237719733466 2315 14-15 -14.798789391421064 2315 16-17 -19.739819163356508 2315 18-19 -22.78777589360674 2315 20-21 -22.27145124326674 2315 22-23 -22.13216062094743 2315 24-25 -21.797055228234203 2315 26-27 -21.4600060615991 2315 28-29 -21.351049383999793 2315 30-31 -21.205781954399985 2315 32-33 -20.88734779408446 2315 34-35 -18.168760920114817 2315 36-37 -16.571065167673126 2315 38-39 -15.694504070553762 2315 40-41 -16.844395985316687 2315 42-43 -16.750867213734164 2315 44-45 -14.885552107696501 2315 46-47 -15.58352250940154 2315 48-49 -15.04162566916445 2315 50-51 -13.218760661964533 2315 52-53 -15.887657144017467 2315 54-55 -17.318493059608993 2315 56-57 -17.4609205058424 2315 58-59 -17.085642112687037 2315 60-61 -16.763827298627834 2315 62-63 -16.28365742318009 2315 64-65 -15.767012931430603 2315 66-67 -15.356145239036938 2315 68-69 -14.898711551687633 2315 70-71 -14.311903772487344 2315 72-73 -13.832991219476865 2315 74-75 -13.303023394375495 2315 76-77 -12.745159851998492 2315 78-79 -12.264288600536803 2315 80-81 -11.767087304612202 2315 82-83 -11.298063316824084 2315 84-85 -10.795232488508775 2315 86-87 -10.222225081821774 2315 88-89 -9.705928129417766 2315 90-91 -9.169224992964125 2315 92-93 -8.549968084367865 2315 94-95 -7.9325707522380515 2315 96-97 -7.256364504159716 2315 98-99 -6.510915073640575 2315 100-101 -5.593973722332255 2316 1 -20.249172789999648 2316 2 -19.149411271318826 2316 3 -19.554374770884547 2316 4 -21.453698680448486 2316 5 -21.41072213040252 2316 6 -22.137674763175255 2316 7 -22.17752734266244 2316 8 -22.91483156773252 2316 9 -24.00639234506293 2316 10-11 -24.200718934715937 2316 12-13 -24.10673929864798 2316 14-15 -24.263142111871346 2316 16-17 -23.4133651295854 2316 18-19 -22.78777589360674 2316 20-21 -22.27145124326674 2316 22-23 -22.13216062094743 2316 24-25 -21.797055228234203 2316 26-27 -21.4600060615991 2316 28-29 -21.351049383999793 2316 30-31 -21.205781954399985 2316 32-33 -20.88734779408446 2316 34-35 -18.168760920114817 2316 36-37 -16.571065167673126 2316 38-39 -15.694504070553762 2316 40-41 -16.844395985316687 2316 42-43 -16.750867213734164 2316 44-45 -14.885552107696501 2316 46-47 -15.58352250940154 2316 48-49 -15.04162566916445 2316 50-51 -13.218760661964533 2316 52-53 -15.887657144017467 2316 54-55 -17.318493059608993 2316 56-57 -17.4609205058424 2316 58-59 -17.085642112687037 2316 60-61 -16.763827298627834 2316 62-63 -16.28365742318009 2316 64-65 -15.767012931430603 2316 66-67 -15.356145239036938 2316 68-69 -14.898711551687633 2316 70-71 -14.311903772487344 2316 72-73 -13.832991219476865 2316 74-75 -13.303023394375495 2316 76-77 -12.745159851998492 2316 78-79 -12.264288600536803 2316 80-81 -11.767087304612202 2316 82-83 -11.298063316824084 2316 84-85 -10.795232488508775 2316 86-87 -10.222225081821774 2316 88-89 -9.705928129417766 2316 90-91 -9.169224992964125 2316 92-93 -8.549968084367865 2316 94-95 -7.9325707522380515 2316 96-97 -7.256364504159716 2316 98-99 -6.510915073640575 2316 100-101 -5.593973722332255 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 351922.0 3 23109.0 4 45665.0 5 30122.0 6 27694.0 7 33763.0 8 42103.0 9 48558.0 10 49284.0 11 44979.0 12 39530.0 13 35918.0 14 34403.0 15 36047.0 16 38941.0 17 40714.0 18 42174.0 19 43827.0 20 45472.0 21 48181.0 22 50348.0 23 52692.0 24 55440.0 25 57739.0 26 61267.0 27 64297.0 28 67036.0 29 69114.0 30 70775.0 31 69398.0 32 66131.0 33 62968.0 34 55824.0 35 44421.0 36 28002.0 37 10227.0 38 1018.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57596097264445 19.668359367867108 13.587593900149674 24.16808575933877 2 15.735849662189269 20.70810264310818 39.99345240960955 23.562595285093 3 18.619854485558204 25.652463657938725 29.944798565323115 25.782883291179953 4 12.990417534266536 16.813125981030215 38.08703254499648 32.10942393970677 5 13.756831293200989 37.36488426259705 34.92192158253475 13.956362861667207 6 30.98948168632222 36.79106331256213 18.11965822246573 14.099796778649909 7 27.251960140692468 32.19647841843733 22.80405235107171 17.74750908979849 8 25.69745173824846 35.08269226029333 20.89159641660185 18.328259584856365 9 26.691886148102107 15.765098742000658 20.854325066697935 36.6886900431993 10-11 25.250169032220192 25.81716408767434 28.43974173007675 20.492925150028718 12-13 25.614037650714778 23.661456618614988 28.38955305102689 22.33495267964335 14-15 22.944835770458617 24.909840281504493 26.08853291322717 26.056791034809716 16-17 22.765389844171636 27.734995001559877 26.741796979074866 22.757818175193616 18-19 22.674757059546412 27.05988572541348 28.34051053270605 21.924846682334056 20-21 23.474052969873664 26.255244145594208 28.245143123512158 22.02555976101997 22-23 23.280746157571624 26.045459243032997 27.962439689079783 22.711354910315595 24-25 23.045315476224204 26.263853818081394 28.002196152438746 22.688634553255653 26-27 22.94923337275152 26.43237029577542 27.982336250163275 22.636060081309783 28-29 22.87896951742048 26.597121105806863 27.969703413522794 22.55420596324986 30-31 23.151223181398453 26.46020069043128 27.82136590662641 22.567210221543856 32-33 23.11328661275831 26.524377358490565 27.692334231805933 22.670001796945193 34-35 23.30563769892361 26.425459975997047 27.75379596991239 22.515106355166953 36-37 23.777954028959375 26.496025889684233 27.3084620138345 22.417558067521895 38-39 23.616354106456594 26.522713763838425 27.362426294683722 22.498505835021263 40-41 23.61509355172591 26.284498132414562 27.538947678968086 22.561460636891443 42-43 23.697199374889593 26.34477830308007 27.23197221840949 22.72605010362085 44-45 23.85664681396009 26.6065903256428 26.93399200138602 22.60277085901109 46-47 23.305285599002953 26.412184925441125 27.216909800983636 23.06561967457229 48-49 23.87669247698077 26.56111288832703 26.76824419203696 22.793950442655238 50-51 23.97893262203828 26.701640238538243 26.625544387858984 22.693882751564495 52-53 23.00453047308179 26.829310536728745 27.06114378260998 23.105015207579484 54-55 22.959074754714077 26.716451845625063 27.167393793321697 23.157079606339163 56-57 23.025446475844955 26.784378294759232 26.9759635084553 23.21421172094051 58-59 22.982573496321475 26.74275224999432 27.16908534870764 23.105588904976564 60-61 23.032865185431902 26.857268088689047 27.1057403629534 23.004126362925646 62-63 23.028132705541807 26.769709713852375 26.97906346852122 23.223094112084603 64-65 22.832984931728113 26.67203509453661 27.038939438983682 23.456040534751597 66-67 22.963066478845917 26.742361221309295 26.88667022680532 23.40790207303947 68-69 22.891684189737095 26.673617807312056 26.97959385201622 23.455104150934627 70-71 22.867291568963168 26.47729835069348 26.970595640544857 23.684814439798497 72-73 23.11886945614126 26.56274161482189 26.88715983115726 23.43122909787959 74-75 23.099665220185386 26.751318147599022 26.66839702972012 23.48061960249547 76-77 23.025754384110385 26.550973266396316 26.775346967957127 23.64792538153617 78-79 22.91944327384399 26.609489972236783 26.747106903801765 23.72395985011746 80-81 23.007308061701103 26.685799013318913 26.65634414249079 23.650548782489196 82-83 23.203962530948505 26.445308805945476 26.776268029266063 23.57446063383995 84-85 23.20839491111495 26.537771942060445 26.554030718996756 23.699802427827848 86-87 22.926803246700374 26.599247003596528 26.672132114089344 23.80181763561375 88-89 23.07463812613139 26.396447347095307 26.60229485444007 23.926619672333228 90-91 23.068297454720337 26.36245308257827 26.561924062906577 24.007325399794816 92-93 23.120142579839435 26.24310520823817 26.615385662918516 24.021366549003876 94-95 22.944557240746192 26.190846797879004 26.651790431414607 24.2128055299602 96-97 22.987374326147933 26.15474398920424 26.480934313995636 24.376947370652196 98-99 22.812400977310737 26.16445039250725 26.43451163103049 24.588636999151525 100-101 23.339774850066682 26.151126907670903 26.191318130901937 24.317780111360474 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23879.0 1 26141.0 2 19518.5 3 11087.0 4 14139.5 5 18022.5 6 19295.0 7 18638.0 8 17209.5 9 15448.5 10 13846.5 11 12411.0 12 10705.0 13 8924.5 14 7481.0 15 6393.5 16 5571.5 17 5102.0 18 4873.0 19 4831.0 20 5015.5 21 5507.5 22 6258.0 23 7209.5 24 8366.0 25 10006.5 26 12006.5 27 14397.0 28 17341.0 29 20587.0 30 24090.0 31 27717.0 32 31635.5 33 35405.5 34 38672.5 35 42063.0 36 45740.0 37 49271.0 38 51824.0 39 53350.0 40 54389.5 41 55768.0 42 57261.0 43 58988.5 44 60695.5 45 62257.5 46 63680.5 47 64255.0 48 64268.0 49 63683.5 50 62244.0 51 60436.0 52 58484.5 53 56189.0 54 53291.0 55 49154.5 56 44279.5 57 39299.0 58 34816.0 59 30528.5 60 26254.0 61 22257.0 62 18436.5 63 14854.5 64 11797.5 65 9354.0 66 7384.5 67 5744.5 68 4400.0 69 3498.0 70 2858.5 71 2337.0 72 1854.5 73 1484.5 74 1222.5 75 945.5 76 706.0 77 531.5 78 393.5 79 273.5 80 170.0 81 116.5 82 77.5 83 49.5 84 39.0 85 19.5 86 12.5 87 8.5 88 4.5 89 3.5 90 2.5 91 1.0 92 1.0 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.651054292732512 2 21.682152685678894 3 17.469255032160177 4 22.714676844773482 5 22.59809180606765 6 16.408728343285876 7 15.226689779227451 8 12.29220564414365 9 3.1049124581595953 10-11 6.646780010718382 12-13 3.6443005048499177 14-15 7.583331406169405 16-17 11.691584058365919 18-19 14.805822314145148 20-21 15.229957578932446 22-23 13.16724699437838 24-25 15.71246077383508 26-27 13.209225652127138 28-29 13.233834697597816 30-31 14.114556991935071 32-33 12.57064731592987 34-35 19.425133703281773 36-37 24.221308467220954 38-39 23.243281152437326 40-41 18.374360139680842 42-43 18.321446921380762 44-45 23.683654868066355 46-47 13.030879198996937 48-49 21.73659925614824 50-51 21.48000156854386 52-53 11.46957477379285 54-55 13.102016684882557 56-57 12.64377061855929 58-59 13.6460801988431 60-61 13.058027073469185 62-63 13.021930455189418 64-65 14.094271496843305 66-67 12.400772005111845 68-69 13.451570705633586 70-71 12.043224443543984 72-73 11.405852679545484 74-75 11.482369466483938 76-77 11.539933015133432 78-79 11.958538157343048 80-81 11.928650358502765 82-83 10.422546611392255 84-85 10.328961855225414 86-87 10.468069574974887 88-89 11.906756100479312 90-91 9.367901961986941 92-93 11.398336740224 94-95 11.502856056942164 96-97 11.054890991229225 98-99 10.914476151597754 100-101 11.000871748413608 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1989106.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.38522329021241 #Duplication Level Percentage of deduplicated Percentage of total 1 98.80072694801446 96.21730855067695 2 0.8699313933638738 1.6943692597981292 3 0.17376894164715573 0.5076758154963645 4 0.06023350233176437 0.2346341229652161 5 0.02978216409010194 0.14501713499901617 6 0.01445647043102147 0.08447079605480273 7 0.012023038852805189 0.08196064263151327 8 0.007439840747669017 0.057962444196429444 9 0.004424566646471726 0.038779866974616786 >10 0.024617468270926184 0.41215439566089623 >50 0.0014535660836583386 0.09514492316096901 >100 9.865228801821183E-4 0.16194769935273112 >500 1.0371775993771007E-4 0.0843903225953529 >1k 5.185887996885504E-5 0.18418402543701498 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3647 0.1833487003709204 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.027384161527842E-5 0.0 9 0.0 0.0 0.0 5.027384161527842E-5 0.0 10-11 0.0 0.0 0.0 5.027384161527842E-5 0.0 12-13 0.0 0.0 0.0 5.027384161527842E-5 7.541076242291763E-5 14-15 0.0 2.513692080763921E-5 0.0 5.027384161527842E-5 1.7595844565347448E-4 16-17 0.0 5.027384161527842E-5 0.0 5.027384161527842E-5 3.016430496916705E-4 18-19 0.0 5.027384161527842E-5 0.0 5.027384161527842E-5 4.021907329222274E-4 20-21 0.0 5.027384161527842E-5 0.0 5.027384161527842E-5 4.021907329222274E-4 22-23 0.0 5.027384161527842E-5 0.0 7.541076242291763E-5 4.021907329222274E-4 24-25 0.0 5.027384161527842E-5 0.0 2.513692080763921E-4 4.021907329222274E-4 26-27 0.0 5.027384161527842E-5 0.0 5.027384161527842E-4 4.273276537298666E-4 28-29 0.0 5.027384161527842E-5 0.0 0.001080887594728486 4.524645745375058E-4 30-31 0.0 5.027384161527842E-5 0.0 0.003770538121145882 4.77601495345145E-4 32-33 0.0 5.027384161527842E-5 0.0 0.010456959055977912 5.278753369604235E-4 34-35 0.0 5.027384161527842E-5 0.0 0.018777279843306488 6.03286099383341E-4 36-37 0.0 5.027384161527842E-5 0.0 0.03240149092104694 6.03286099383341E-4 38-39 0.0 5.027384161527842E-5 0.0 0.05286294445846526 6.03286099383341E-4 40-41 2.513692080763921E-5 5.027384161527842E-5 0.0 0.07510911937322595 6.03286099383341E-4 42-43 5.027384161527842E-5 5.027384161527842E-5 0.0 0.0986624141699839 6.03286099383341E-4 44-45 1.0054768323055685E-4 5.027384161527842E-5 0.0 0.12867589761430512 6.284230201909803E-4 46-47 1.0054768323055685E-4 5.027384161527842E-5 0.0 0.16595395117203407 6.535599409986195E-4 48-49 1.0054768323055685E-4 5.027384161527842E-5 0.0 0.2059719290977957 6.535599409986195E-4 50-51 1.0054768323055685E-4 5.027384161527842E-5 0.0 0.25332988789938793 6.535599409986195E-4 52-53 1.0054768323055685E-4 5.027384161527842E-5 0.0 0.3158454099479867 6.535599409986195E-4 54-55 1.2568460403819605E-4 5.027384161527842E-5 0.0 0.38534899598110905 6.535599409986195E-4 56-57 1.5082152484583526E-4 5.027384161527842E-5 0.0 0.46445488576274974 6.535599409986195E-4 58-59 1.5082152484583526E-4 5.027384161527842E-5 0.0 0.5522330132230258 6.535599409986195E-4 60-61 1.5082152484583526E-4 5.027384161527842E-5 0.0 0.6451139356072527 6.535599409986195E-4 62-63 1.5082152484583526E-4 5.027384161527842E-5 0.0 0.7438266236188519 7.541076242291763E-4 64-65 1.5082152484583526E-4 5.027384161527842E-5 0.0 0.8487229941491303 7.541076242291763E-4 66-67 1.5082152484583526E-4 5.027384161527842E-5 0.0 0.9671681649947262 7.792445450368155E-4 68-69 1.5082152484583526E-4 5.027384161527842E-5 0.0 1.093229822845037 8.043814658444548E-4 70-71 1.5082152484583526E-4 5.027384161527842E-5 0.0 1.228717825998212 8.043814658444548E-4 72-73 1.5082152484583526E-4 5.027384161527842E-5 0.0 1.3663173304992293 8.797922282673724E-4 74-75 1.5082152484583526E-4 5.027384161527842E-5 0.0 1.510276475964579 9.5520299069029E-4 76-77 1.5082152484583526E-4 5.027384161527842E-5 0.0 1.6751746764626922 9.5520299069029E-4 78-79 1.5082152484583526E-4 5.027384161527842E-5 0.0 1.8589004306457273 9.5520299069029E-4 80-81 1.5082152484583526E-4 5.027384161527842E-5 0.0 2.0575575157885 9.803399114979292E-4 82-83 1.5082152484583526E-4 5.027384161527842E-5 0.0 2.2589293883784975 0.0010054768323055684 84-85 1.5082152484583526E-4 5.027384161527842E-5 0.0 2.463066322257336 0.0010054768323055684 86-87 1.5082152484583526E-4 5.027384161527842E-5 0.0 2.68555320832575 0.0010306137531132077 88-89 1.5082152484583526E-4 5.027384161527842E-5 0.0 2.921488347026252 0.001055750673920847 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1570 0.0 57.171906 1 GTATCAA 2955 0.0 43.003654 1 ATCAACG 3610 0.0 35.31919 3 TCAACGC 3655 0.0 35.002068 4 CAACGCA 3655 0.0 34.65661 5 TATCAAC 3860 0.0 32.516045 2 AACGCAG 4035 0.0 31.619944 6 GTACATG 4545 0.0 30.067526 1 TACATGG 4765 0.0 27.82137 2 ACGCAGA 4605 0.0 27.724625 7 CGCAGAG 5025 0.0 26.25835 8 ACATGGG 5045 0.0 25.972248 3 GCAGAGT 5215 0.0 23.481915 9 CATGGGG 3720 0.0 20.850916 4 CAGAGTA 4995 0.0 19.553194 10-11 GAGTACA 3380 0.0 19.394962 1 AGAGTAC 4695 0.0 19.249464 10-11 GAGTACT 2855 0.0 18.86996 12-13 AGTACAT 3290 0.0 18.691734 2 GTATTAG 465 0.0 18.43564 1 >>END_MODULE