FastQCFastQC Report
Sat 18 Jun 2016
SRR1930934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989106
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.14810673739861022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.13392951406310172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22700.053.9780651
GTATCAA42300.042.66451
ATCAACG51850.033.7868843
CAACGCA51650.033.7220085
TCAACGC53350.032.8369264
TATCAAC54550.032.5513652
AACGCAG55400.031.867716
ACGCAGA63700.027.3428847
CGCAGAG64850.026.6384588
GTACATG58850.025.5014041
TACATGG62750.023.6065882
ACATGGG62050.023.1065833
GCAGAGT74300.022.8671429
AGAGTAC68750.019.98451210-11
CAGAGTA71800.019.92877210-11
CATGGGG45400.019.8686474
GAGTACT44250.019.30531512-13
GTACTTT48100.017.90808914-15
AGTACTT47350.017.8409412-13
GTGGTAT9900.017.7496571
Produced by FastQC (version 0.11.5)