##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930934_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1989106 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.231433116183855 31.0 31.0 33.0 30.0 34.0 2 31.35161474551884 31.0 31.0 34.0 30.0 34.0 3 31.431832692677013 31.0 31.0 34.0 28.0 34.0 4 35.30793834013873 37.0 35.0 37.0 33.0 37.0 5 35.08204791499297 35.0 35.0 37.0 32.0 37.0 6 35.11646387874754 37.0 35.0 37.0 32.0 37.0 7 34.9897416226184 36.0 35.0 37.0 32.0 37.0 8 34.97190194992122 36.0 35.0 37.0 32.0 37.0 9 36.476403469699456 38.0 35.0 39.0 32.0 39.0 10-11 36.40018430390336 38.0 35.0 39.0 32.0 39.0 12-13 36.29626953013062 38.0 35.0 39.0 32.0 39.0 14-15 37.30292377580682 39.0 36.0 41.0 32.0 41.0 16-17 37.264365750241566 39.0 36.0 40.5 32.0 41.0 18-19 37.24810216247902 39.0 36.0 40.0 31.5 41.0 20-21 37.20572508453546 39.0 36.0 40.0 31.0 41.0 22-23 37.07530493598632 39.0 36.0 40.0 31.0 41.0 24-25 36.94542925314187 39.0 36.0 40.0 31.0 41.0 26-27 36.66272963834004 38.5 35.0 40.0 30.0 41.0 28-29 36.474285432752204 38.0 35.0 40.0 30.0 41.0 30-31 36.23625764539446 38.0 35.0 40.0 30.0 41.0 32-33 36.00099542206398 38.0 34.0 40.0 29.5 41.0 34-35 36.207073931706006 38.0 35.0 40.0 30.0 41.0 36-37 36.26313906850615 38.0 35.0 40.0 30.0 41.0 38-39 36.22243761770363 38.0 35.0 40.0 30.0 41.0 40-41 36.118534155545255 38.0 35.0 40.0 30.0 41.0 42-43 35.9727852613184 38.0 35.0 40.0 29.0 41.0 44-45 35.77281653164789 38.0 34.0 40.0 28.5 41.0 46-47 35.539650727512765 38.0 34.0 40.0 28.0 41.0 48-49 35.31793378532869 38.0 34.0 40.0 27.0 41.0 50-51 35.04040483513699 38.0 33.5 40.0 26.5 41.0 52-53 34.81862982666585 38.0 33.0 40.0 26.0 41.0 54-55 34.631664677498335 37.5 33.0 40.0 26.0 41.0 56-57 34.36239647359166 37.0 33.0 40.0 26.0 41.0 58-59 34.10229570470352 37.0 33.0 40.0 25.0 41.0 60-61 33.78193419556323 36.5 32.0 39.5 24.5 41.0 62-63 33.46032790610455 36.0 32.0 39.0 23.5 41.0 64-65 33.14766583580764 35.5 31.5 39.0 23.0 40.0 66-67 32.80232300339952 35.0 31.0 39.0 22.5 40.0 68-69 32.43229445791225 35.0 31.0 38.0 22.0 40.0 70-71 32.04197287625697 35.0 31.0 37.5 21.5 40.0 72-73 31.618452460552632 34.5 30.0 37.0 20.5 39.0 74-75 31.102190381005336 34.0 30.0 36.5 19.5 39.0 76-77 29.737410173213494 32.5 28.5 35.0 18.0 37.5 78-79 30.51323232648235 34.0 29.5 36.0 19.0 38.0 80-81 30.519472315703638 34.0 30.0 35.0 19.0 37.0 82-83 30.253871337173585 34.0 30.0 35.0 18.0 37.0 84-85 29.938714930224933 34.0 29.0 35.0 17.5 36.0 86-87 29.603194098253184 34.0 29.0 35.0 13.5 36.0 88-89 29.26723663796701 34.0 29.0 35.0 8.5 36.0 90-91 28.93012891218467 33.5 29.0 35.0 6.0 35.0 92-93 28.57475167235934 33.0 29.0 35.0 2.0 35.0 94-95 28.15339504279812 33.0 27.5 35.0 2.0 35.0 96-97 27.682563674334098 33.0 27.0 35.0 2.0 35.0 98-99 27.17543760865434 33.0 27.0 35.0 2.0 35.0 100-101 25.939778724713513 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5275205374940413 1101 2 0.5478545650072562 1101 3 0.6198243727142838 1101 4 0.39717127257575413 1101 5 0.500094567720538 1101 6 0.4434371914926274 1101 7 0.5284155300699709 1101 8 0.47100159540256925 1101 9 0.5569201859626318 1101 10-11 0.5612856794804131 1101 12-13 0.6132916434637607 1101 14-15 0.7684521763162664 1101 16-17 0.8845210118322697 1101 18-19 0.8266557674525146 1101 20-21 0.7786658435630969 1101 22-23 0.8525623444512718 1101 24-25 0.7425003873629734 1101 26-27 0.7502807737147066 1101 28-29 0.7477485671962683 1101 30-31 0.7379357439368874 1101 32-33 0.7489366232184906 1101 34-35 0.6017662674581814 1101 36-37 0.6773960532629033 1101 38-39 0.8323089982310279 1101 40-41 0.7979366639677394 1101 42-43 0.5694800585004387 1101 44-45 0.7003135114073586 1101 46-47 0.677724458022162 1101 48-49 0.74438407329977 1101 50-51 0.6363818418329146 1101 52-53 0.6912064350805949 1101 54-55 0.6347273017501536 1101 56-57 0.6539806477094601 1101 58-59 0.7898997803190468 1101 60-61 0.7452358188493378 1101 62-63 0.7813890385267399 1101 64-65 0.9202011580344944 1101 66-67 0.7374028199863787 1101 68-69 0.8075321371630224 1101 70-71 0.6754267260621063 1101 72-73 0.8373530143135248 1101 74-75 0.7054503151435263 1101 76-77 0.948065634577631 1101 78-79 0.8395162207667681 1101 80-81 0.7383690555881657 1101 82-83 0.8928648468407978 1101 84-85 0.9460482528790699 1101 86-87 0.8858796443464563 1101 88-89 0.8534306227201007 1101 90-91 0.8717004767395267 1101 92-93 1.2168324258494962 1101 94-95 1.094671952532451 1101 96-97 1.1325082719506092 1101 98-99 1.2697023331261477 1101 100-101 1.1985719853204522 1102 1 0.4326683521398209 1102 2 0.5351037529518301 1102 3 0.4513701561935832 1102 4 0.2924135529096219 1102 5 0.41549483909095386 1102 6 0.45188822146672436 1102 7 0.5195204394075787 1102 8 0.555297060227204 1102 9 0.67192779255776 1102 10-11 0.6409925171926787 1102 12-13 0.6666830027828681 1102 14-15 0.8323948595940891 1102 16-17 0.7720143303635822 1102 18-19 0.7333845203820815 1102 20-21 0.8225929024833789 1102 22-23 0.7483545610542066 1102 24-25 0.753141911149001 1102 26-27 0.8599717300898604 1102 28-29 0.9276866865170206 1102 30-31 0.8488938665469732 1102 32-33 0.7344581375476764 1102 34-35 0.7459955343468749 1102 36-37 0.7514197878955287 1102 38-39 0.7015279859371262 1102 40-41 0.6787212739755475 1102 42-43 0.765306453385513 1102 44-45 0.8254065380098794 1102 46-47 0.829272759901194 1102 48-49 0.7797832653560235 1102 50-51 0.6761612505743315 1102 52-53 0.8124632707370196 1102 54-55 0.8404661693953912 1102 56-57 0.6806325123531778 1102 58-59 0.851725208960957 1102 60-61 0.791350801785903 1102 62-63 0.6959708299826843 1102 64-65 0.7171952680628593 1102 66-67 0.8069435461078314 1102 68-69 0.9469689921443134 1102 70-71 0.8563346862611141 1102 72-73 0.6967792817367418 1102 74-75 0.9078394541591948 1102 76-77 1.0206744397254468 1102 78-79 1.029372209418554 1102 80-81 0.789219914927827 1102 82-83 1.0732693840719953 1102 84-85 1.065515482844944 1102 86-87 1.0087305242444877 1102 88-89 1.018130367796374 1102 90-91 1.1610877346647754 1102 92-93 1.1957593305820282 1102 94-95 0.9961567804589393 1102 96-97 0.9601770047329801 1102 98-99 1.1835476211488434 1102 100-101 1.4494840057965845 1103 1 0.4617671348943375 1103 2 0.5411497528597451 1103 3 0.47239096544494075 1103 4 0.29949698620588094 1103 5 0.3400958961534286 1103 6 0.43864149843308553 1103 7 0.46027623180458477 1103 8 0.43861500532877784 1103 9 0.489262696217736 1103 10-11 0.5564519952529352 1103 12-13 0.5486831336434221 1103 14-15 0.7466376980079659 1103 16-17 0.6735331609548965 1103 18-19 0.5349922876829325 1103 20-21 0.7441436969765647 1103 22-23 0.6337425262453067 1103 24-25 0.6612881017740548 1103 26-27 0.7942376872015231 1103 28-29 0.865371739091465 1103 30-31 0.8137774370044752 1103 32-33 0.8162382288107963 1103 34-35 0.7223899868287447 1103 36-37 0.7876938486681411 1103 38-39 0.8331629189195553 1103 40-41 1.0344996155846005 1103 42-43 0.942457594901505 1103 44-45 1.0421180365328553 1103 46-47 0.9524253845034636 1103 48-49 0.7365894681462564 1103 50-51 0.8138046216391217 1103 52-53 0.7721359254856921 1103 54-55 0.890253884497433 1103 56-57 0.9244103353667086 1103 58-59 0.8379539532085474 1103 60-61 0.7789923553430071 1103 62-63 0.6969720343844443 1103 64-65 0.5787404403989385 1103 66-67 0.5572109620723396 1103 68-69 0.650648112373652 1103 70-71 0.6834941883124728 1103 72-73 0.6696042702864453 1103 74-75 0.7808400139515612 1103 76-77 0.8207628917662646 1103 78-79 0.7702170596922713 1103 80-81 0.727693160002989 1103 82-83 0.5540675365148502 1103 84-85 0.6014379164635031 1103 86-87 0.7137178865076983 1103 88-89 0.4979583346353387 1103 90-91 0.7584825191447173 1103 92-93 0.6577016889315388 1103 94-95 0.6137553439008592 1103 96-97 0.565137225548046 1103 98-99 0.837756968954384 1103 100-101 0.9614402983717021 1104 1 0.3619604458879486 1104 2 0.494709339477037 1104 3 0.43199354527228806 1104 4 0.2651930290230311 1104 5 0.3670934724302839 1104 6 0.35474382297724816 1104 7 0.3615956057445686 1104 8 0.4058587098106159 1104 9 0.42031956861721653 1104 10-11 0.44322832609995544 1104 12-13 0.46976518224699504 1104 14-15 0.6289500355216759 1104 16-17 0.585616799943395 1104 18-19 0.6057078435254013 1104 20-21 0.6045973381364291 1104 22-23 0.5337240989071077 1104 24-25 0.570632170694644 1104 26-27 0.6506035109446202 1104 28-29 0.40041161608603915 1104 30-31 0.44916719868147936 1104 32-33 0.6240871011633331 1104 34-35 0.48965434870880387 1104 36-37 0.5659795193586987 1104 38-39 0.4838986626739228 1104 40-41 0.5708797586605598 1104 42-43 0.5316548319744925 1104 44-45 0.5901424967703051 1104 46-47 0.6430424655896161 1104 48-49 0.5825291743651917 1104 50-51 0.6317154075005433 1104 52-53 0.7552373521372502 1104 54-55 0.5452117187570238 1104 56-57 0.6195277949762144 1104 58-59 0.5264833459866765 1104 60-61 0.6683003911882821 1104 62-63 0.6941731916682272 1104 64-65 0.7504302977337787 1104 66-67 0.7775004003906432 1104 68-69 0.8156712639179702 1104 70-71 0.6428664850982528 1104 72-73 0.668548747660136 1104 74-75 0.8558703838480959 1104 76-77 0.8940485331139243 1104 78-79 0.8998181723748573 1104 80-81 0.7962702056429265 1104 82-83 0.7481935583811747 1104 84-85 0.9573862494936272 1104 86-87 0.8589523068974891 1104 88-89 0.8630689280073689 1104 90-91 0.8966982363731049 1104 92-93 0.9829534803152882 1104 94-95 1.0208969351073804 1104 96-97 0.939172988668723 1104 98-99 1.075942873801246 1104 100-101 1.3877469081522769 1105 1 0.1286214083558619 1105 2 0.2708592365702067 1105 3 0.2382572180054474 1105 4 0.11688046976488664 1105 5 0.1003388531855478 1105 6 0.17039167349640394 1105 7 0.30828200585557397 1105 8 0.19166751123512427 1105 9 0.2974818839052418 1105 10-11 0.20736124934764888 1105 12-13 0.008749064031327691 1105 14-15 0.45825524098845705 1105 16-17 0.18933058020840576 1105 18-19 0.37802313154889333 1105 20-21 0.3228533564515317 1105 22-23 0.4269791922170114 1105 24-25 0.42881465109609707 1105 26-27 0.506978299927809 1105 28-29 0.46288831751748916 1105 30-31 0.6431908556895536 1105 32-33 0.5043572026826553 1105 34-35 0.5222565039709295 1105 36-37 0.5634687083603822 1105 38-39 0.4332016712533431 1105 40-41 0.5183081285701405 1105 42-43 0.3423793422998642 1105 44-45 0.36556013661952136 1105 46-47 0.5079663839116577 1105 48-49 0.5494727259226693 1105 50-51 0.5432499911325763 1105 52-53 0.4404638627863804 1105 54-55 0.6093291481675607 1105 56-57 0.6713225363216324 1105 58-59 0.4677012309622768 1105 60-61 0.5186917840148695 1105 62-63 0.5441619759801455 1105 64-65 0.46838557373221335 1105 66-67 0.4603172724855469 1105 68-69 0.5186819246029373 1105 70-71 0.518396975582256 1105 72-73 0.5325994610703937 1105 74-75 0.7957115142044557 1105 76-77 0.6603436560239899 1105 78-79 0.6148142411027422 1105 80-81 0.5862195616080541 1105 82-83 0.7910060367013649 1105 84-85 0.726162583235503 1105 86-87 0.8205536065297956 1105 88-89 0.722957002377786 1105 90-91 0.529971482910117 1105 92-93 0.4844841326752096 1105 94-95 0.3549737799146335 1105 96-97 0.20891340908358913 1105 98-99 0.4391639731699257 1105 100-101 0.7463965161813206 1106 1 0.024746206494111078 1106 2 0.10856646770666956 1106 3 0.046247046529440894 1106 4 0.05589783123470937 1106 5 0.08819225850944434 1106 6 0.12480127658771778 1106 7 0.03636539310932818 1106 8 0.10410119817768049 1106 9 0.11475191556876752 1106 10-11 0.1932010214137918 1106 12-13 0.1148141600277981 1106 14-15 0.17837811611601495 1106 16-17 0.1766617730369049 1106 18-19 0.24366386022676778 1106 20-21 0.16282094076223075 1106 22-23 0.21971588474735881 1106 24-25 0.10333852017187439 1106 26-27 0.18603828118705934 1106 28-29 0.1831029479858799 1106 30-31 0.14373481759908202 1106 32-33 0.2535207564644182 1106 34-35 0.2233939530183946 1106 36-37 0.23804966964824104 1106 38-39 0.2622766505460916 1106 40-41 0.22340578343956707 1106 42-43 0.32496848317988025 1106 44-45 0.2655556508028596 1106 46-47 0.04098403356122304 1106 48-49 0.1891415729681043 1106 50-51 0.1496535623863693 1106 52-53 0.09077497003092816 1106 54-55 0.2958774980213903 1106 56-57 0.21683816961925828 1106 58-59 0.3828162128649524 1106 60-61 0.17864943609312434 1106 62-63 0.22374484940399242 1106 64-65 0.3119615217801126 1106 66-67 0.1748399000322607 1106 68-69 0.2648209504829353 1106 70-71 0.18457450698750932 1106 72-73 0.06204431633710783 1106 74-75 0.13750627585806185 1106 76-77 0.1853736067796916 1106 78-79 0.2862795988171669 1106 80-81 0.18030344909833573 1106 82-83 0.38018452508083556 1106 84-85 0.3577159535790919 1106 86-87 0.28141666859356107 1106 88-89 0.412291995916398 1106 90-91 0.3970855078385682 1106 92-93 0.5039725139569384 1106 94-95 0.5805598360027489 1106 96-97 0.3324149650729247 1106 98-99 0.41032379692717313 1106 100-101 0.6782100638913775 1107 1 0.19239715723879414 1107 2 0.1635773339347537 1107 3 0.19502834419348147 1107 4 0.060051934593253975 1107 5 0.21066334111467455 1107 6 0.1774174612121513 1107 7 0.10692817121706355 1107 8 0.017667649307362865 1107 9 0.0981466744850863 1107 10-11 0.15105366436736034 1107 12-13 0.11125498433275993 1107 14-15 0.23602990255250944 1107 16-17 0.39438272972974175 1107 18-19 0.32026283585629045 1107 20-21 0.31320703485040724 1107 22-23 0.30417460894537385 1107 24-25 0.2875268958327766 1107 26-27 0.3420917735074127 1107 28-29 0.4766410392210858 1107 30-31 0.4292966326501997 1107 32-33 0.5053959237703722 1107 34-35 0.4280952110697456 1107 36-37 0.35962014908061235 1107 38-39 0.38807407706218555 1107 40-41 0.3474611053440313 1107 42-43 0.44159832577934566 1107 44-45 0.5346328861555705 1107 46-47 0.5262288219948843 1107 48-49 0.563214547186341 1107 50-51 0.5558751985815675 1107 52-53 0.48308390161010806 1107 54-55 0.5296775898134172 1107 56-57 0.45878893798649045 1107 58-59 0.41089869788171285 1107 60-61 0.5509419845375447 1107 62-63 0.2596047427661432 1107 64-65 0.4215831723652954 1107 66-67 0.2947025739689977 1107 68-69 0.5560256963186632 1107 70-71 0.24916666927081366 1107 72-73 0.39960012781397225 1107 74-75 0.6784711513288109 1107 76-77 0.6780545999748355 1107 78-79 0.6849298448099823 1107 80-81 0.7188483934533281 1107 82-83 0.8742863060875727 1107 84-85 0.8186122799761826 1107 86-87 0.8662946911054057 1107 88-89 0.9822389540652203 1107 90-91 0.9380388757883154 1107 92-93 0.9243593498891869 1107 94-95 1.035368907236311 1107 96-97 1.029738449755854 1107 98-99 1.0654630558070721 1107 100-101 1.2929201615064407 1108 1 0.24860256993970964 1108 2 0.2554188441624454 1108 3 0.24025213518750377 1108 4 0.09805893709977909 1108 5 0.17080163842760498 1108 6 0.12809054319143343 1108 7 0.2211649944103442 1108 8 0.17767821003373285 1108 9 0.23860827901436465 1108 10-11 0.258609294417667 1108 12-13 0.25132921616042125 1108 14-15 0.3448257606443903 1108 16-17 0.4073688079755229 1108 18-19 0.2720108332621223 1108 20-21 0.3404446161373329 1108 22-23 0.3654220016291134 1108 24-25 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-0.9897142548918971 2316 84-85 -0.9009783520869483 2316 86-87 -0.9789901399152896 2316 88-89 -1.0827531171098386 2316 90-91 -0.9847245468797752 2316 92-93 -1.1727585130521874 2316 94-95 -1.0679245885009259 2316 96-97 -1.003326509880413 2316 98-99 -1.0555225662051768 2316 100-101 -1.0134270217515784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 45.0 5 93.0 6 156.0 7 451.0 8 1042.0 9 1667.0 10 2555.0 11 3905.0 12 5338.0 13 6896.0 14 8210.0 15 9857.0 16 11819.0 17 13789.0 18 12567.0 19 10965.0 20 11265.0 21 11901.0 22 13407.0 23 16039.0 24 19739.0 25 24633.0 26 31647.0 27 40107.0 28 50941.0 29 62546.0 30 76370.0 31 91071.0 32 108890.0 33 131016.0 34 160922.0 35 198632.0 36 255797.0 37 313590.0 38 243990.0 39 37218.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.635982169570106 20.86849177995057 14.192086929631149 24.30343912084818 2 15.774581608890283 21.544879761574382 39.005142158459726 23.67539647107561 3 18.427473340167445 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22.85355617403615 26.383261005960474 28.08543444686653 22.67774837313685 24-25 22.742410938835796 26.60687905287652 28.177541914130362 22.473168094157323 26-27 22.663542592036777 26.802765514921806 28.020294921895623 22.513396971145795 28-29 22.556275309749864 26.89413077740994 28.034970501808605 22.514623411031597 30-31 22.935122525113375 26.67957948295106 27.937938038592648 22.447359953342918 32-33 22.804377592495094 26.756083076503778 27.824056544238296 22.61548278676283 34-35 22.8684566657105 26.752793641942702 27.964928414535763 22.41382127781103 36-37 22.915080936345895 26.622924808983385 27.92771482896233 22.534279425708387 38-39 22.824115608441737 26.693089088069517 27.96263725159224 22.520158051896505 40-41 22.857915655197186 26.55943519091793 28.05099289287938 22.5316562610055 42-43 22.83508015819466 26.681471857902345 27.745856842482088 22.737591141420914 44-45 22.74471908349816 26.81552715648527 27.66738195051268 22.77237180950389 46-47 22.91132936691989 26.732641159244537 27.524113847981635 22.831915625853934 48-49 22.978686711680968 26.830292869484424 27.399771786094334 22.791248632740274 50-51 22.949697210224343 26.863627755930892 27.236524545513152 22.950150488331612 52-53 22.896049159355208 26.88966834184503 27.244521467608145 22.969761031191616 54-55 22.834778502995473 26.882141886505178 27.293907760723247 22.989171849776103 56-57 22.93448478798253 26.90865750467154 27.120683773403904 23.036173933942028 58-59 22.780446878635374 26.87630219758531 27.372454091367477 22.97079683241184 60-61 22.91062441086434 27.009097210276035 27.27447861808517 22.805799760774455 62-63 22.900758557198998 26.922905639650985 27.16084638166279 23.015489421487228 64-65 22.657274739044393 26.834728325619256 27.22108226910917 23.28691466622718 66-67 22.743347021760574 26.874433842623347 27.162846991565516 23.219372144050567 68-69 22.709157231546 26.864661406299106 27.209858873894856 23.21632248826004 70-71 22.76898606389159 26.658697808782133 27.28826441847926 23.284051708847016 72-73 22.84831021892761 26.795019010115674 27.150721353082556 23.205949417874162 74-75 22.78638845764105 26.96557054971124 26.959977345001462 23.288063647646243 76-77 22.678856347784006 26.937934963161254 26.920593150488116 23.462615538566627 78-79 22.62846771385728 26.88046214816947 27.063546630232445 23.427523507740805 80-81 22.67661123019104 26.980431035544584 26.962533435456905 23.380424298807473 82-83 22.802329287629718 26.797264700825394 27.089632226739045 23.31077378480584 84-85 22.83637041876361 26.81788371707785 26.77716189513327 23.568583969025273 86-87 22.5607383417475 26.996173155176244 26.9354926283466 23.507595874729653 88-89 22.638495844609356 26.724810127982312 26.966024383855437 23.6706696435529 90-91 22.64641047446981 26.661315538023473 26.98658137037997 23.705692617126743 92-93 22.67041776188842 26.711594328605493 26.923837933578966 23.69414997592712 94-95 22.41274903843519 26.6337803668086 27.02559555934403 23.92787503541218 96-97 22.50450704990081 26.5429293360937 26.993307546204175 23.959256067801313 98-99 22.27246813392549 26.63264300645617 26.921667321902405 24.17322153771594 100-101 22.356123806373315 26.593404273075443 26.847412857836638 24.20305906271461 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 124.0 1 87.5 2 73.0 3 100.0 4 92.5 5 98.5 6 173.5 7 316.5 8 467.5 9 558.5 10 536.5 11 521.5 12 586.0 13 683.0 14 835.5 15 1018.5 16 1173.0 17 1337.5 18 1558.5 19 1776.5 20 2015.0 21 2385.0 22 2877.5 23 3538.5 24 4570.5 25 6083.0 26 8199.0 27 10711.5 28 13675.0 29 17672.5 30 22113.0 31 26413.0 32 31424.5 33 35864.0 34 39665.5 35 43770.0 36 48874.0 37 54456.5 38 57529.0 39 58684.5 40 59764.5 41 60973.5 42 62522.5 43 65554.5 44 68853.5 45 71971.0 46 74516.0 47 76508.0 48 78297.5 49 78297.5 50 75966.5 51 73936.0 52 73520.0 53 72127.0 54 69347.5 55 64414.5 56 58065.0 57 51343.0 58 45141.5 59 39802.0 60 34105.0 61 28193.0 62 22860.5 63 18076.0 64 13873.5 65 10599.5 66 8050.5 67 6358.0 68 4982.0 69 3867.5 70 3167.5 71 2483.5 72 1988.0 73 1746.0 74 1405.0 75 1106.5 76 862.5 77 600.0 78 393.0 79 275.5 80 222.5 81 150.5 82 89.0 83 57.5 84 38.5 85 24.5 86 11.0 87 4.0 88 4.5 89 5.0 90 3.5 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018651595239268296 2 0.0029158828136861487 3 0.0032677997049930974 4 0.0337840215654671 5 0.002061227506226415 6 1.0054768323055683E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0054768323055683E-4 24-25 4.7760149534514497E-4 26-27 0.0018601321397653016 28-29 5.0273841615278416E-5 30-31 0.006334504043525081 32-33 0.021768573419415557 34-35 0.01304606189916475 36-37 6.03286099383341E-4 38-39 6.284230201909803E-4 40-41 0.0022371859518798896 42-43 0.00824491002490566 44-45 0.0076416239255223195 46-47 0.007817582371175795 48-49 0.009174976094788312 50-51 0.17947761456654396 52-53 0.048237751029859645 54-55 0.0178220768526162 56-57 0.010130179085478603 58-59 0.020134673566919007 60-61 0.0514804138140451 62-63 0.0928306485426116 64-65 0.09745584197121722 66-67 0.1018548031125541 68-69 0.09966789100228947 70-71 0.0898142180456949 72-73 0.06779427541820295 74-75 0.2289973485575932 76-77 0.27454544906103545 78-79 0.006711557855639669 80-81 6.284230201909803E-4 82-83 0.0 84-85 2.5136920807639208E-5 86-87 0.0 88-89 1.5082152484583526E-4 90-91 0.005907176389795214 92-93 0.01777180301100092 94-95 0.0015333521692659918 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1989106.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.64109436313839 #Duplication Level Percentage of deduplicated Percentage of total 1 79.71321782355139 49.93323199674487 2 11.96444789100877 14.989322186870652 3 3.608425053945004 6.781070829194452 4 1.595077189577552 3.996695229952681 5 0.8213586400457406 2.5725402038542127 6 0.5246800157889299 1.9719918227692375 7 0.33113717818545385 1.451995665810087 8 0.24535425718861295 1.2295407341559703 9 0.17976424035779015 1.01345658690332 >10 0.9315432649313353 10.304776795077892 >50 0.058920764672474375 2.5161994544017556 >100 0.02510862564465862 2.57088044340553 >500 7.237913266354876E-4 0.30281468292740715 >1k 2.4126377554515227E-4 0.36548336793183617 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2946 0.14810673739861022 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2664 0.13392951406310172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.027384161527842E-5 2 0.0 0.0 0.0 0.0 5.027384161527842E-5 3 0.0 0.0 0.0 0.0 5.027384161527842E-5 4 0.0 0.0 0.0 0.0 5.027384161527842E-5 5 0.0 0.0 0.0 0.0 5.027384161527842E-5 6 0.0 0.0 0.0 0.0 5.027384161527842E-5 7 0.0 0.0 0.0 0.0 5.027384161527842E-5 8 0.0 0.0 0.0 0.0 5.027384161527842E-5 9 0.0 0.0 0.0 0.0 5.027384161527842E-5 10-11 0.0 0.0 0.0 0.0 5.027384161527842E-5 12-13 0.0 0.0 0.0 0.0 2.513692080763921E-4 14-15 0.0 0.0 0.0 0.0 2.513692080763921E-4 16-17 0.0 0.0 0.0 0.0 2.513692080763921E-4 18-19 0.0 0.0 0.0 0.0 3.016430496916705E-4 20-21 0.0 0.0 0.0 5.027384161527842E-5 4.77601495345145E-4 22-23 0.0 0.0 0.0 1.2568460403819605E-4 5.027384161527842E-4 24-25 0.0 0.0 0.0 4.021907329222274E-4 5.027384161527842E-4 26-27 0.0 0.0 0.0 8.043814658444548E-4 5.278753369604235E-4 28-29 0.0 0.0 0.0 0.002136638268649333 5.530122577680626E-4 30-31 0.0 0.0 0.0 0.008496279232982053 5.530122577680626E-4 32-33 0.0 0.0 0.0 0.024307402420987115 6.03286099383341E-4 34-35 0.0 0.0 0.0 0.04607597584040267 6.535599409986195E-4 36-37 0.0 0.0 0.0 0.07955835435617811 6.535599409986195E-4 38-39 0.0 0.0 0.0 0.12867589761430512 6.535599409986195E-4 40-41 0.0 0.0 0.0 0.18256945582588358 6.535599409986195E-4 42-43 0.0 0.0 0.0 0.23834828309803502 6.535599409986195E-4 44-45 0.0 0.0 0.0 0.3084551552305408 7.038337826138979E-4 46-47 0.0 0.0 0.0 0.3906026124299057 7.792445450368155E-4 48-49 0.0 0.0 0.0 0.4759424585718408 8.043814658444548E-4 50-51 0.0 0.0 0.0 0.5672900287868017 8.043814658444548E-4 52-53 0.0 0.0 0.0 0.6603217726958744 8.546553074597332E-4 54-55 0.0 0.0 0.0 0.7657711554839208 9.049291490750116E-4 56-57 0.0 0.0 0.0 0.8844676955375932 9.803399114979292E-4 58-59 0.0 5.027384161527842E-5 0.0 1.0166124882233525 0.001055750673920847 60-61 0.0 5.027384161527842E-5 0.0 1.1580328046871307 0.001080887594728486 62-63 0.0 5.027384161527842E-5 0.0 1.3042291361043605 0.0011060245155361252 64-65 0.0 7.541076242291763E-5 0.0 1.465557893847789 0.0012819829611895998 66-67 0.0 2.010953664611137E-4 0.0 1.6486300880898253 0.0013322568028048781 68-69 0.0 2.010953664611137E-4 0.0 1.851258806720205 0.0013573937236125175 70-71 0.0 2.010953664611137E-4 0.0 2.067008998012172 0.0013825306444201566 72-73 0.0 2.010953664611137E-4 0.0 2.2884652703274737 0.0015836260108812701 74-75 0.0 2.010953664611137E-4 0.0 2.5266124580590477 0.0016590367733041878 76-77 0.0 2.513692080763921E-4 0.0 2.7983425719896275 0.0016590367733041878 78-79 0.0 2.513692080763921E-4 0.0 3.0991309663738384 0.0016590367733041878 80-81 0.0 2.513692080763921E-4 0.0 3.4279470274585666 0.0016841736941118272 82-83 0.0 2.513692080763921E-4 0.0 3.767949018302695 0.0017847213773423839 84-85 0.0 2.513692080763921E-4 0.0 4.123988364622097 0.0018601321397653016 86-87 0.0 2.513692080763921E-4 0.0 4.514063101715042 0.002010953664611137 88-89 0.0 2.513692080763921E-4 0.0 4.9453121150909 0.002111501347841694 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2270 0.0 53.978065 1 GTATCAA 4230 0.0 42.6645 1 ATCAACG 5185 0.0 33.786884 3 CAACGCA 5165 0.0 33.722008 5 TCAACGC 5335 0.0 32.836926 4 TATCAAC 5455 0.0 32.551365 2 AACGCAG 5540 0.0 31.86771 6 ACGCAGA 6370 0.0 27.342884 7 CGCAGAG 6485 0.0 26.638458 8 GTACATG 5885 0.0 25.501404 1 TACATGG 6275 0.0 23.606588 2 ACATGGG 6205 0.0 23.106583 3 GCAGAGT 7430 0.0 22.867142 9 AGAGTAC 6875 0.0 19.984512 10-11 CAGAGTA 7180 0.0 19.928772 10-11 CATGGGG 4540 0.0 19.868647 4 GAGTACT 4425 0.0 19.305315 12-13 GTACTTT 4810 0.0 17.908089 14-15 AGTACTT 4735 0.0 17.84094 12-13 GTGGTAT 990 0.0 17.749657 1 >>END_MODULE