FastQCFastQC Report
Sat 18 Jun 2016
SRR1930933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154513
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58030.1839586649349678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.054.2493361
GTATCAA49250.045.902691
ATCAACG64550.034.3826373
TATCAAC65800.033.660222
TCAACGC66250.033.414764
CAACGCA67150.031.9861095
GTACATG96700.031.7948511
TACATGG99550.030.7241762
AACGCAG72250.029.4090926
ACATGGG104000.029.2102433
GTGGTAT12250.026.6568681
ACGCAGA83850.025.0837067
CATGGGG77450.024.9531254
CGCAGAG90750.023.4740318
GAGTACA66800.021.6594731
TGGTATC14600.020.983662
AGTACAT66550.020.9797842
CATGGGA35950.020.8052544
GCAGAGT95350.020.7469739
GTATAAG11400.018.9493981
Produced by FastQC (version 0.11.5)