FastQCFastQC Report
Sat 18 Jun 2016
SRR1930933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3154513
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51830.16430428405272066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46630.14781996460309405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37700.055.1746331
GTATCAA70350.044.4864771
CAACGCA91800.033.9182745
ATCAACG91950.033.7736173
TCAACGC92800.033.7702064
TATCAAC97000.032.26162
AACGCAG97450.032.0486456
GTACATG144250.028.2473771
TACATGG147150.027.6562182
ACGCAGA117750.026.3622537
ACATGGG150450.026.2277133
CGCAGAG121200.025.5335168
CATGGGG101000.022.9422634
GCAGAGT133250.022.4764569
GAGTACA102850.021.6558511
AGTACAT103350.020.5844752
GTGGTAT17550.020.2950821
CATGGGA49000.020.0591284
GTATACG6650.019.9959961
AGAGTAC127100.019.26947210-11
Produced by FastQC (version 0.11.5)