##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930933_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3154513 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22483470507175 31.0 31.0 33.0 30.0 34.0 2 31.33800399617944 31.0 31.0 34.0 30.0 34.0 3 31.418224619774907 31.0 31.0 34.0 28.0 34.0 4 35.28642202457242 37.0 35.0 37.0 33.0 37.0 5 35.07279475468955 35.0 35.0 37.0 32.0 37.0 6 35.11729195600081 37.0 35.0 37.0 32.0 37.0 7 34.98926331893386 36.0 35.0 37.0 32.0 37.0 8 34.96511252291558 36.0 35.0 37.0 32.0 37.0 9 36.465874764187056 38.0 35.0 39.0 32.0 39.0 10-11 36.389690421310675 38.0 35.0 39.0 32.0 39.0 12-13 36.269718812380866 38.0 35.0 39.0 32.0 39.0 14-15 37.273703262595525 39.0 36.0 40.5 32.0 41.0 16-17 37.230213031298334 39.0 36.0 40.0 31.5 41.0 18-19 37.206669619050544 39.0 36.0 40.0 31.0 41.0 20-21 37.15445189162321 39.0 36.0 40.0 31.0 41.0 22-23 37.01901561350357 39.0 36.0 40.0 31.0 41.0 24-25 36.87765908715545 39.0 35.5 40.0 31.0 41.0 26-27 36.58564491571282 38.0 35.0 40.0 30.0 41.0 28-29 36.382776675829206 38.0 35.0 40.0 30.0 41.0 30-31 36.13701623673765 38.0 35.0 40.0 29.5 41.0 32-33 35.89047342648453 38.0 34.0 40.0 29.0 41.0 34-35 36.11078366137657 38.0 35.0 40.0 30.0 41.0 36-37 36.17235798362536 38.0 35.0 40.0 30.0 41.0 38-39 36.12230778570258 38.0 35.0 40.0 30.0 41.0 40-41 36.0102139379359 38.0 35.0 40.0 29.0 41.0 42-43 35.84371914143324 38.0 34.5 40.0 29.0 41.0 44-45 35.63002640978179 38.0 34.0 40.0 28.0 41.0 46-47 35.36974265124284 38.0 34.0 40.0 27.0 41.0 48-49 35.12714625046719 38.0 33.5 40.0 27.0 41.0 50-51 34.82150319241036 38.0 33.0 40.0 26.0 41.0 52-53 34.571230329372554 37.5 33.0 40.0 26.0 41.0 54-55 34.35466615607544 37.0 33.0 40.0 25.5 41.0 56-57 34.05409789086303 37.0 32.5 40.0 25.0 41.0 58-59 33.777486572412286 36.5 32.0 39.5 24.0 41.0 60-61 33.42906401083147 36.0 32.0 39.0 23.5 41.0 62-63 33.0922563958367 35.5 31.0 39.0 23.0 40.0 64-65 32.759649746252435 35.0 31.0 39.0 22.5 40.0 66-67 32.39231491517074 35.0 31.0 38.0 22.0 40.0 68-69 31.99858615260105 35.0 31.0 37.5 21.5 40.0 70-71 31.593727938353716 34.5 30.0 37.0 20.0 39.5 72-73 31.175121801685396 34.0 30.0 36.5 19.5 39.0 74-75 30.649284374481894 34.0 29.0 36.0 18.0 39.0 76-77 29.318347554757263 32.5 28.0 35.0 16.5 37.0 78-79 30.096651686013026 34.0 29.0 35.0 18.0 37.0 80-81 30.11352956858951 34.0 29.0 35.0 18.0 37.0 82-83 29.86142139848528 34.0 29.0 35.0 16.5 36.5 84-85 29.562245107247932 34.0 29.0 35.0 13.0 36.0 86-87 29.23391233448713 34.0 29.0 35.0 8.5 36.0 88-89 28.90774328715716 33.0 29.0 35.0 7.0 35.0 90-91 28.57209702416823 33.0 29.0 35.0 2.0 35.0 92-93 28.198900908000695 33.0 27.5 35.0 2.0 35.0 94-95 27.77222775750171 33.0 27.0 35.0 2.0 35.0 96-97 27.286522991029045 33.0 26.5 35.0 2.0 35.0 98-99 26.76513268450629 33.0 25.0 35.0 2.0 35.0 100-101 25.503871596027658 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.477192751663317 1101 2 0.5770823691799869 1101 3 0.5507940533549487 1101 4 0.3912592220167781 1101 5 0.430521486114678 1101 6 0.4039665895251403 1101 7 0.5480335866077937 1101 8 0.4663744626346329 1101 9 0.7104487493730147 1101 10-11 0.6696267375113436 1101 12-13 0.6464288329911767 1101 14-15 0.8423288319589659 1101 16-17 0.7617336482544133 1101 18-19 0.7667875609970167 1101 20-21 0.6885181390321833 1101 22-23 0.8220823231467449 1101 24-25 0.7757031340754139 1101 26-27 0.8087442977781834 1101 28-29 0.8312479819931369 1101 30-31 0.875912433249276 1101 32-33 0.8688889803494959 1101 34-35 0.7742963554879836 1101 36-37 0.7621021201455846 1101 38-39 0.8881278428412216 1101 40-41 0.8818129761317834 1101 42-43 0.9117861044751336 1101 44-45 0.8251274740753374 1101 46-47 0.9050714433600859 1101 48-49 0.9668729526549171 1101 50-51 0.922745678039739 1101 52-53 0.8353171957423484 1101 54-55 0.8217626426229643 1101 56-57 0.9135449253643415 1101 58-59 0.9510905855874583 1101 60-61 0.9059401151417106 1101 62-63 0.8976398506185532 1101 64-65 1.0009594397332222 1101 66-67 1.060236210170352 1101 68-69 0.9165471013417701 1101 70-71 0.940383938863409 1101 72-73 0.8661401648906022 1101 74-75 0.8406977451308855 1101 76-77 0.8933388269073106 1101 78-79 1.001134111389458 1101 80-81 0.9311471861233791 1101 82-83 1.0973138200282655 1101 84-85 1.2061384173263576 1101 86-87 1.0467577546306437 1101 88-89 0.9687366038871268 1101 90-91 0.967487060375241 1101 92-93 1.1336525167408027 1101 94-95 1.074760192942474 1101 96-97 1.0337319205790543 1101 98-99 1.0636340282939152 1101 100-101 1.3119153028324426 1102 1 0.454402541644928 1102 2 0.47536664467552114 1102 3 0.49243471468823685 1102 4 0.2705955508489737 1102 5 0.4086264476203354 1102 6 0.4095913073748889 1102 7 0.39583076186025323 1102 8 0.5084525442531884 1102 9 0.6713134567381687 1102 10-11 0.625274152880614 1102 12-13 0.583291092753349 1102 14-15 0.7765411652819054 1102 16-17 0.6822253668869322 1102 18-19 0.698658248961344 1102 20-21 0.7477415432226451 1102 22-23 0.7481698050523704 1102 24-25 0.7192891306023057 1102 26-27 0.6485550298682128 1102 28-29 0.7636462169651779 1102 30-31 0.7950634563767238 1102 32-33 0.8227071477596581 1102 34-35 0.8490180778030236 1102 36-37 0.9176534565203127 1102 38-39 1.0241002379243653 1102 40-41 0.9070086570770926 1102 42-43 0.9557298289695595 1102 44-45 0.9270110096245716 1102 46-47 0.9488796281645122 1102 48-49 0.8992514662434843 1102 50-51 1.0754095349471129 1102 52-53 0.9345445298666917 1102 54-55 0.986550399301251 1102 56-57 0.981125982939659 1102 58-59 0.907647604947762 1102 60-61 0.8291678171594938 1102 62-63 0.8329176765045432 1102 64-65 0.8308740712427536 1102 66-67 0.8356190649732795 1102 68-69 0.8293055276614254 1102 70-71 0.7939950676042464 1102 72-73 0.7399879430616103 1102 74-75 0.9330160511285861 1102 76-77 1.1149615489530547 1102 78-79 1.0679375189576632 1102 80-81 0.9792830492440636 1102 82-83 1.144767143995736 1102 84-85 1.1465555461008954 1102 86-87 1.1660218350709393 1102 88-89 1.175583079371112 1102 90-91 1.097892915689851 1102 92-93 1.1167295975887939 1102 94-95 1.1020136209671314 1102 96-97 1.1960836381860531 1102 98-99 1.1902964064561026 1102 100-101 1.4459115256496524 1103 1 0.4786047277823613 1103 2 0.5585635500561708 1103 3 0.5110519782504142 1103 4 0.4013265198792553 1103 5 0.3977141572174503 1103 6 0.4532942441535184 1103 7 0.4467921963977446 1103 8 0.4918633474366132 1103 9 0.61408385428701 1103 10-11 0.6558258772503365 1103 12-13 0.6508480479963126 1103 14-15 0.7397593047778699 1103 16-17 0.7256412964004184 1103 18-19 0.7424332250057404 1103 20-21 0.755487334210251 1103 22-23 0.7497572780564425 1103 24-25 0.7920286675733479 1103 26-27 0.8283191779996955 1103 28-29 0.8180722139328864 1103 30-31 0.945041601370491 1103 32-33 0.9739688210836519 1103 34-35 0.9147935537442038 1103 36-37 0.9137989799057067 1103 38-39 0.9073761963384683 1103 40-41 0.9263502491439795 1103 42-43 0.8920211591213061 1103 44-45 0.9807964364481521 1103 46-47 0.9625148613002068 1103 48-49 0.9588040143992345 1103 50-51 1.0297196145891974 1103 52-53 0.9794338370461873 1103 54-55 1.060144811070792 1103 56-57 0.9999225265408 1103 58-59 1.070588307035628 1103 60-61 1.0254442583118575 1103 62-63 0.9222561393520081 1103 64-65 0.8881751380606957 1103 66-67 0.721295182448678 1103 68-69 0.8582995411055663 1103 70-71 0.7069859185732632 1103 72-73 0.7399201492037619 1103 74-75 0.8653258962118819 1103 76-77 0.977491658182096 1103 78-79 1.0870363267586711 1103 80-81 1.080897639791278 1103 82-83 0.975588920532676 1103 84-85 0.987568866598945 1103 86-87 1.0666228610091437 1103 88-89 1.090228091627079 1103 90-91 1.128271860801501 1103 92-93 1.1800548540335463 1103 94-95 1.2487109058695616 1103 96-97 1.2620580291778616 1103 98-99 1.3979154277940573 1103 100-101 1.6619073559515023 1104 1 0.44630856010758535 1104 2 0.5133100466099236 1104 3 0.4956000165032002 1104 4 0.3308141019902564 1104 5 0.3990529003873675 1104 6 0.3909448625469025 1104 7 0.48132836003602364 1104 8 0.40050632692963006 1104 9 0.5797216776745202 1104 10-11 0.5771705185804308 1104 12-13 0.5851995244067183 1104 14-15 0.6530456935118636 1104 16-17 0.5731669834099407 1104 18-19 0.6330581951017251 1104 20-21 0.5755873158546194 1104 22-23 0.5582384061187184 1104 24-25 0.5813812270529866 1104 26-27 0.6963324462502811 1104 28-29 0.564324533397432 1104 30-31 0.6539175929877956 1104 32-33 0.7681025875286878 1104 34-35 0.7765983994568657 1104 36-37 0.7584826323457392 1104 38-39 0.7298153030542878 1104 40-41 0.6847510821938556 1104 42-43 0.5964376457839009 1104 44-45 0.7439404168015429 1104 46-47 0.6929887323636379 1104 48-49 0.6094221033083471 1104 50-51 0.6705766833775755 1104 52-53 0.800397968750886 1104 54-55 0.7336671610091585 1104 56-57 0.830993782057277 1104 58-59 0.7224620603098515 1104 60-61 0.6629832640282061 1104 62-63 0.5675124265906746 1104 64-65 0.6888189770496282 1104 66-67 0.6162192849583548 1104 68-69 0.6249562760128526 1104 70-71 0.761068741617148 1104 72-73 0.6437519959629476 1104 74-75 0.6849519371980044 1104 76-77 0.760631445448162 1104 78-79 0.8736197087358804 1104 80-81 0.7420982285633713 1104 82-83 0.9087435401926847 1104 84-85 0.8949880213525745 1104 86-87 0.8977944635427413 1104 88-89 0.8282055881451633 1104 90-91 0.7689954815411042 1104 92-93 0.7931016626330241 1104 94-95 0.8872892230844478 1104 96-97 0.8531110943507905 1104 98-99 1.0844483535337837 1104 100-101 1.4825223391922009 1105 1 0.16957259446033035 1105 2 0.24537260770474134 1105 3 0.1929170811589742 1105 4 0.09223708760679017 1105 5 0.2042372321846031 1105 6 0.18546553005143807 1105 7 0.27642855553943946 1105 8 0.22547752508631191 1105 9 0.37944500542204196 1105 10-11 0.3672373737682477 1105 12-13 0.30619737736021335 1105 14-15 0.47827523547527306 1105 16-17 0.31962261111556955 1105 18-19 0.3929228264381308 1105 20-21 0.365522817181251 1105 22-23 0.43315069141407037 1105 24-25 0.45144476566491676 1105 26-27 0.43895961075244117 1105 28-29 0.5385817949530818 1105 30-31 0.440913560729733 1105 32-33 0.5511020813144754 1105 34-35 0.5015281404148269 1105 36-37 0.5121919606518475 1105 38-39 0.33728545387121045 1105 40-41 0.41239561508032807 1105 42-43 0.41088438855725684 1105 44-45 0.5397362204602487 1105 46-47 0.4851066279089551 1105 48-49 0.5091956160858899 1105 50-51 0.47904006139740574 1105 52-53 0.34454857620794854 1105 54-55 0.40071392472930967 1105 56-57 0.5104270839704128 1105 58-59 0.5845933094370679 1105 60-61 0.5326322306529718 1105 62-63 0.5298182323440628 1105 64-65 0.5636204188873606 1105 66-67 0.6043110319210641 1105 68-69 0.6870862159653655 1105 70-71 0.6601479554141072 1105 72-73 0.6090728310418463 1105 74-75 0.7378024469033555 1105 76-77 0.9030748891012834 1105 78-79 0.7953410989048493 1105 80-81 0.7274930756517684 1105 82-83 0.985561983845809 1105 84-85 0.9625628188668891 1105 86-87 0.882444390228958 1105 88-89 0.9540251315050305 1105 90-91 0.8654777291322233 1105 92-93 0.9102487964132244 1105 94-95 0.8027243927534862 1105 96-97 0.727130522503284 1105 98-99 1.043566755293316 1105 100-101 1.2706354693416237 1106 1 0.09355368689730525 1106 2 0.22635299986160362 1106 3 0.15177562457634153 1106 4 -0.01622229614671511 1106 5 0.07625403890729388 1106 6 0.18945012388897453 1106 7 0.1350069869119892 1106 8 0.10450063433000878 1106 9 0.09007875892343975 1106 10-11 0.15556020289990613 1106 12-13 0.13193792357870393 1106 14-15 0.21879694415874695 1106 16-17 0.15017233100352456 1106 18-19 0.27559614576586 1106 20-21 0.22309809729329544 1106 22-23 0.25516224603592264 1106 24-25 0.15237613821393126 1106 26-27 0.19811052111658256 1106 28-29 0.18570714509314 1106 30-31 0.17822973720031854 1106 32-33 0.4040660168887058 1106 34-35 0.11676098355208353 1106 36-37 0.19406871135212356 1106 38-39 0.17429175639221484 1106 40-41 0.29247964869377086 1106 42-43 0.2292930020026347 1106 44-45 0.20396241093643397 1106 46-47 0.004136739953771951 1106 48-49 0.1811126328926136 1106 50-51 0.10830826868032517 1106 52-53 0.007594794695336304 1106 54-55 0.18121287430914634 1106 56-57 0.3001347170236386 1106 58-59 0.3519480013138221 1106 60-61 0.06874917743169107 1106 62-63 -0.05120242591925006 1106 64-65 0.06789737967167042 1106 66-67 -0.03619316975960629 1106 68-69 0.08600078179169657 1106 70-71 -0.019407366714744967 1106 72-73 0.015956584543246777 1106 74-75 0.16119180264565003 1106 76-77 0.16240262019371698 1106 78-79 0.18688346585162918 1106 80-81 0.18577038657613798 1106 82-83 0.2850735384676568 1106 84-85 0.2645498636848167 1106 86-87 0.2400546843466067 1106 88-89 0.25180874495040584 1106 90-91 0.2614178551826427 1106 92-93 0.34434368436840757 1106 94-95 0.3496046448543275 1106 96-97 0.4126819930915211 1106 98-99 0.4908094023521379 1106 100-101 0.8495552872687924 1107 1 0.09614102858244422 1107 2 0.12655870177427175 1107 3 0.14482695553817493 1107 4 0.008319471480582763 1107 5 0.10228718836339823 1107 6 0.13637482014785007 1107 7 0.11882192392974389 1107 8 0.13797204743512737 1107 9 0.1461025426700644 1107 10-11 0.17258903897420907 1107 12-13 0.1296243785287814 1107 14-15 0.1203928954226896 1107 16-17 0.1940657165786135 1107 18-19 0.14618476771771327 1107 20-21 0.14638846865656774 1107 22-23 0.23229526488763952 1107 24-25 0.1762694808270453 1107 26-27 0.3221550898643315 1107 28-29 0.33924093897879004 1107 30-31 0.3369559577498933 1107 32-33 0.41018311403431085 1107 34-35 0.23843929291263777 1107 36-37 0.18582985139096309 1107 38-39 0.25415479363164906 1107 40-41 0.17195229051124272 1107 42-43 0.32548506778599773 1107 44-45 0.39361987514912045 1107 46-47 0.3221081616512862 1107 48-49 0.4078672856739658 1107 50-51 0.3186270464982002 1107 52-53 0.2681134315979534 1107 54-55 0.4414229153807909 1107 56-57 0.26623978481762833 1107 58-59 0.36775044499067633 1107 60-61 0.36564603433395604 1107 62-63 0.274558774266346 1107 64-65 0.22762220825341473 1107 66-67 0.29260346545889604 1107 68-69 0.3782818046302836 1107 70-71 0.3992821213504172 1107 72-73 0.23317814801526282 1107 74-75 0.5120976557844514 1107 76-77 0.5626534751369228 1107 78-79 0.5372259586769808 1107 80-81 0.6015691784855406 1107 82-83 0.6252849972843144 1107 84-85 0.5815602261119963 1107 86-87 0.42509794705046033 1107 88-89 0.5341869048033026 1107 90-91 0.5923355658251808 1107 92-93 0.714328733602823 1107 94-95 0.5900984067762742 1107 96-97 0.5002471234381396 1107 98-99 0.5493721526056135 1107 100-101 0.855225923621493 1108 1 0.28697257537878684 1108 2 0.36258839874065885 1108 3 0.37654034791892954 1108 4 0.18207934686133598 1108 5 0.24897390690792065 1108 6 0.15442463194687406 1108 7 0.2855670239276904 1108 8 0.31908176330875904 1108 9 0.3066073151366666 1108 10-11 0.40799634209289337 1108 12-13 0.4177858362076847 1108 14-15 0.4724646409792399 1108 16-17 0.46617812780132084 1108 18-19 0.4598106534745554 1108 20-21 0.5302772218371459 1108 22-23 0.566020634805497 1108 24-25 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-1.3997835626403514 2316 56-57 -1.2987450244892216 2316 58-59 -1.3417345552302393 2316 60-61 -0.8853881659932696 2316 62-63 -1.250212738848031 2316 64-65 -1.1423074404053395 2316 66-67 -0.744283507474826 2316 68-69 -0.9739680138529678 2316 70-71 -0.7416518672237551 2316 72-73 -0.7208946525655904 2316 74-75 -0.35932616752209867 2316 76-77 -0.8801684195088448 2316 78-79 -0.733160795645297 2316 80-81 -0.6818681519092316 2316 82-83 -0.9958260764573197 2316 84-85 -0.8763920209697211 2316 86-87 -0.6710192102225072 2316 88-89 -0.9166194186400851 2316 90-91 -0.6210148673420335 2316 92-93 -0.7948742149809469 2316 94-95 -0.41398728627478576 2316 96-97 -0.2863594135388503 2316 98-99 -0.38726234553437777 2316 100-101 -0.6511179300994208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 37.0 4 98.0 5 129.0 6 321.0 7 821.0 8 1748.0 9 2831.0 10 4353.0 11 6439.0 12 8893.0 13 11409.0 14 13407.0 15 17021.0 16 19877.0 17 23275.0 18 21482.0 19 19050.0 20 19542.0 21 20845.0 22 23286.0 23 27419.0 24 33223.0 25 41508.0 26 53126.0 27 66920.0 28 83558.0 29 102981.0 30 124776.0 31 149142.0 32 177298.0 33 213874.0 34 262444.0 35 323305.0 36 411439.0 37 488055.0 38 336731.0 39 43834.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.61170890922287 19.589782646123563 13.508528676817733 24.28997976783584 2 16.704898565004054 20.58423159273581 38.2009260615375 24.509943780722626 3 19.719674816954562 24.964716041805627 29.050680825054968 26.264928316184843 4 13.746133840396304 16.318214150596052 36.106133117349536 33.8295188916581 5 14.030667883998426 37.83267394529615 33.85545453392678 14.281203636778637 6 33.23636112336869 35.89391956663979 16.699952322260938 14.16976698773059 7 28.993413563361443 31.65525074710423 21.084395594502226 18.2669400950321 8 27.08662161164021 34.94621832276488 19.319432191276434 18.647727874318477 9 27.07207961168003 15.02251378343148 19.35747906490766 38.547927539980826 10-11 26.033051694508785 25.560918595041453 27.268060077736244 21.137969632713514 12-13 26.26418087356115 22.896244206316474 27.67975595599067 23.159818964131706 14-15 23.42904014487183 24.446582475105107 24.964253589104494 27.16012379091857 16-17 23.40293097539937 27.41321719073594 25.716045551246737 23.46780628261795 18-19 23.319320605114008 26.450723138563703 27.545377051861884 22.6845792044604 20-21 24.087077783480364 25.57296482848541 27.390361047806742 22.949596340227476 22-23 23.866000763034393 25.410281563494557 27.288956559187234 23.434761114283816 24-25 23.590845963178307 25.708186941888396 27.383254836980452 23.317712257952845 26-27 23.48280736392049 25.901207845016945 27.19681745946455 23.41916733159801 28-29 23.3577407084648 26.035065339762642 27.315184508787578 23.29200944298498 30-31 23.723955542846344 25.76230424453083 27.26583052184339 23.247909690779434 32-33 23.577756879198038 25.856459521297104 27.074809376204872 23.490974223299986 34-35 23.68687578835168 25.905951517194055 27.289487413084153 23.117685281370115 36-37 23.7168306549323 25.754891275748186 27.142340419731344 23.385937649588175 38-39 23.679540085227522 25.84197641621209 27.170317934948436 23.308165563611954 40-41 23.660798350522786 25.71597176563678 27.335649846217443 23.287580037622995 42-43 23.614572688043083 25.795061013315124 27.043971022776898 23.546395275864896 44-45 23.508815034857513 25.932957546437052 27.045766532351383 23.512460886354052 46-47 23.711520945660833 25.913068620075386 26.759695791212007 23.615714643051778 48-49 23.815138361530757 26.093198679799766 26.52321010735306 23.56845285131642 50-51 23.72145837395699 26.11190788174117 26.52525293585982 23.641380808442015 52-53 23.843460615385297 26.045483566181055 26.51238423822496 23.598671580208688 54-55 23.587064511777125 26.025717709512236 26.62721274379358 23.760005034917054 56-57 23.68567850105154 26.10655781232835 26.405367219324045 23.802396467296063 58-59 23.6183637825312 26.073820327088765 26.624738876085573 23.683077014294458 60-61 23.691539000008564 26.25473110152318 26.584167695643913 23.46956220282434 62-63 23.64553614521486 26.130008983271946 26.45754459983506 23.76691027167813 64-65 23.42904851679916 26.008811081539157 26.594592827818975 23.967547573842708 66-67 23.489926070355025 26.077130427938904 26.502917707571694 23.930025794134373 68-69 23.505950872634397 26.039195202172905 26.577613154959746 23.87724077023296 70-71 23.388277327689536 25.856846290348294 26.685916499618838 24.06895988234333 72-73 23.546231469820352 26.097154766852032 26.46084275241174 23.895771010915873 74-75 23.468114428236117 26.21861782192374 26.35222069096989 23.961047058870253 76-77 23.45016017480319 26.073813279219106 26.312724535575715 24.163302010401985 78-79 23.363958146089235 26.09013713004925 26.376507661751162 24.169397062110352 80-81 23.35024206007604 26.319188977093095 26.280482189567838 24.050086773263025 82-83 23.497256153326994 26.095105647052335 26.433747459592023 23.97389074002865 84-85 23.44721673361308 26.14303697591356 26.132591623493074 24.27715466698029 86-87 23.198826570060103 26.276861119291635 26.319958104468107 24.204354206180163 88-89 23.253820128815672 26.07831808927384 26.259883674392913 24.407978107517575 90-91 23.323345457957796 26.060302292931496 26.319502004311857 24.296850244798847 92-93 23.352530284818545 25.96314236044506 26.304251811921027 24.380075542815362 94-95 23.07003051710427 25.87044599227031 26.465745885865427 24.59377760475999 96-97 23.069567315145 25.82200485463208 26.438391599590812 24.670036230632114 98-99 22.857109797930775 25.915791122116154 26.37381744820833 24.853281631744743 100-101 22.93030651640998 25.91720179945367 26.25628742059392 24.896204263542423 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 250.0 1 181.0 2 146.5 3 183.0 4 168.0 5 187.5 6 307.5 7 593.0 8 973.0 9 1177.0 10 1075.5 11 964.5 12 1071.0 13 1256.5 14 1457.0 15 1682.5 16 1916.5 17 2177.5 18 2483.5 19 2796.5 20 3153.5 21 3586.0 22 4197.0 23 5044.5 24 6105.0 25 7576.0 26 9638.5 27 12347.5 28 15221.0 29 19298.0 30 24302.0 31 29387.0 32 36623.5 33 43122.0 34 48168.5 35 54303.5 36 62142.0 37 71061.0 38 76563.5 39 79498.0 40 81917.5 41 84455.0 42 88371.5 43 94665.5 44 102024.5 45 109121.5 46 114772.5 47 119174.0 48 124314.5 49 127816.5 50 127443.0 51 126789.0 52 127480.5 53 127398.0 54 125423.0 55 118443.0 56 108388.0 57 97728.5 58 87991.0 59 79419.5 60 69725.5 61 58916.0 62 48629.5 63 38791.5 64 30123.0 65 23307.0 66 17418.0 67 13622.0 68 10701.5 69 7982.0 70 6433.5 71 5121.0 72 4068.5 73 3529.5 74 2935.5 75 2372.5 76 1835.0 77 1265.0 78 814.5 79 519.0 80 345.0 81 238.0 82 171.0 83 112.5 84 64.5 85 32.0 86 19.5 87 9.0 88 4.5 89 4.5 90 2.0 91 1.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019781183339551936 2 0.0025043485317701973 3 0.0033285645042515277 4 0.03772373104818398 5 0.002314144845812967 6 3.170061432620503E-5 7 0.0 8 0.0 9 3.170061432620503E-5 10-11 0.0 12-13 0.0 14-15 1.5850307163102515E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.925153581551257E-5 24-25 4.4380860056687034E-4 26-27 0.0016484319449626616 28-29 6.340122865241006E-5 30-31 0.006181619793609981 32-33 0.020827303612316703 34-35 0.012188886208425833 36-37 6.023116721978955E-4 38-39 4.913595220561779E-4 40-41 0.0018703362452460967 42-43 0.007687398974104719 44-45 0.007703249281267821 46-47 0.007354542523679566 48-49 0.009335830919067381 50-51 0.18040819613043282 52-53 0.05053077923597081 54-55 0.01707078081466141 56-57 0.010413651806158351 58-59 0.01897281767423371 60-61 0.05408124804050578 62-63 0.09827190441123558 64-65 0.10315379901747117 66-67 0.10806739423803295 68-69 0.10576909969938308 70-71 0.09416667485599203 72-73 0.07047046564715377 74-75 0.2258827273813739 76-77 0.2718327678472081 78-79 0.0065937277798506465 80-81 8.717668939706382E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.7435337879412766E-4 90-91 0.005975565800489648 92-93 0.017118331736150714 94-95 0.0016642822521257638 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3154513.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.90156904889385 #Duplication Level Percentage of deduplicated Percentage of total 1 74.35802906192251 38.592983796970266 2 13.762989053475739 14.286414533562821 3 4.7098643362974775 7.333480471837984 4 2.244394003326638 4.659502813463232 5 1.2359982598038046 3.2075124512759903 6 0.7833371254210065 2.4393855542160234 7 0.5132123522185049 1.8645568434789774 8 0.373850261451269 1.5522732126928043 9 0.2737398290025651 1.2786773972768122 >10 1.5791631517645388 14.813129117917697 >50 0.11169346755480414 3.986158647837782 >100 0.051769861192491856 4.850425871150777 >500 0.001224522855907492 0.4146305858099818 >1k 6.734875699637101E-4 0.5561668076399896 >5k 6.122614272397364E-5 0.1647018948690473 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5183 0.16430428405272066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4663 0.14781996460309405 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.510184297861508E-5 7 0.0 0.0 0.0 0.0 9.510184297861508E-5 8 0.0 0.0 0.0 0.0 9.510184297861508E-5 9 0.0 0.0 0.0 0.0 9.510184297861508E-5 10-11 0.0 0.0 0.0 0.0 1.5850307163102514E-4 12-13 0.0 0.0 0.0 0.0 3.487067575882553E-4 14-15 0.0 0.0 0.0 0.0 3.9625767907756286E-4 16-17 0.0 0.0 0.0 0.0 4.2795829340376785E-4 18-19 0.0 0.0 0.0 0.0 5.706110578716905E-4 20-21 0.0 0.0 0.0 3.170061432620503E-5 6.815632080134081E-4 22-23 0.0 0.0 0.0 1.109521501417176E-4 7.449644366658182E-4 24-25 0.0 0.0 0.0 3.170061432620503E-4 7.925153581551257E-4 26-27 0.0 0.0 0.0 7.449644366658182E-4 8.400662796444333E-4 28-29 0.0 0.0 0.0 0.0024567976102808893 9.193178154599458E-4 30-31 0.0 0.0 0.0 0.0088286210898481 9.510184297861508E-4 32-33 0.0 0.0 0.0 0.02250743617160557 0.0010302699656016634 34-35 0.0 0.0 0.0 0.04312868579080194 0.001141222115743381 36-37 0.0 0.0 0.0 0.07666793574792685 0.001141222115743381 38-39 0.0 0.0 0.0 0.12620014563262222 0.0012046233443957911 40-41 0.0 0.0 0.0 0.179045069714406 0.0012046233443957911 42-43 0.0 0.0 0.0 0.2326191079256925 0.0012521742658850987 44-45 0.0 0.0 0.0 0.29673360040044217 0.0013472761088637137 46-47 0.0 0.0 0.0 0.37478051287155895 0.0014423779518423288 48-49 0.0 0.0 0.0 0.45621939107557963 0.0015216294876578412 50-51 0.0 0.0 0.0 0.5437447872302317 0.0016167313306364563 52-53 0.0 0.0 0.0 0.6281793734880787 0.0016801325592888665 54-55 0.0 0.0 0.0 0.7241846839749908 0.001759384095104379 56-57 0.0 0.0 0.0 0.8351843850381977 0.0018227853237567891 58-59 0.0 0.0 0.0 0.9580559661665684 0.0018703362452460967 60-61 0.0 0.0 0.0 1.089090455483937 0.0018703362452460967 62-63 0.0 0.0 0.0 1.2246422823427894 0.0019337374738985066 64-65 0.0 0.0 0.0 1.3673584480393646 0.001949587781061609 66-67 0.0 0.0 0.0 1.5315200793276174 0.002028839316877122 68-69 0.0 0.0 0.0 1.7115003171646463 0.002060539931203327 70-71 0.0 0.0 0.0 1.9145586022311525 0.002123941159855737 72-73 0.0 0.0 0.0 2.126049250708429 0.002266593924323659 74-75 0.0 0.0 0.0 2.3458771607534983 0.0023616957673022743 76-77 0.0 0.0 0.0 2.589290327857263 0.002425096995954685 78-79 0.0 0.0 0.0 2.861614455226528 0.0024567976102808893 80-81 0.0 0.0 0.0 3.1577932948762615 0.002536049146096402 82-83 0.0 0.0 0.0 3.473071754657534 0.0026945522177274275 84-85 0.0 0.0 0.0 3.7917421801717097 0.0027579534463798372 86-87 0.0 0.0 0.0 4.139244314415569 0.0029323068251739647 88-89 0.0 0.0 0.0 4.519841890015987 0.0031066602039680927 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3770 0.0 55.174633 1 GTATCAA 7035 0.0 44.486477 1 CAACGCA 9180 0.0 33.918274 5 ATCAACG 9195 0.0 33.773617 3 TCAACGC 9280 0.0 33.770206 4 TATCAAC 9700 0.0 32.2616 2 AACGCAG 9745 0.0 32.048645 6 GTACATG 14425 0.0 28.247377 1 TACATGG 14715 0.0 27.656218 2 ACGCAGA 11775 0.0 26.362253 7 ACATGGG 15045 0.0 26.227713 3 CGCAGAG 12120 0.0 25.533516 8 CATGGGG 10100 0.0 22.942263 4 GCAGAGT 13325 0.0 22.476456 9 GAGTACA 10285 0.0 21.655851 1 AGTACAT 10335 0.0 20.584475 2 GTGGTAT 1755 0.0 20.295082 1 CATGGGA 4900 0.0 20.059128 4 GTATACG 665 0.0 19.995996 1 AGAGTAC 12710 0.0 19.269472 10-11 >>END_MODULE