FastQCFastQC Report
Sat 18 Jun 2016
SRR1930932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518182
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82210.18195371501192292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31600.046.485461
GTATCAA55000.039.6490061
TCAACGC62100.034.4355854
ATCAACG61500.034.0462383
CAACGCA66350.032.0162665
TATCAAC70800.030.293962
AACGCAG72000.029.839346
ACGCAGA82800.025.9605267
GTACATG86050.025.9288141
CGCAGAG84850.025.7385358
ACATGGG87150.023.9099583
TACATGG89850.023.8710352
GCAGAGT96900.021.5512879
CATGGGG62150.020.2065184
AGAGTAC90000.018.47130210-11
GAGTACA62500.018.3339941
CAGAGTA96000.017.684810-11
GAGTACT57200.017.5475212-13
ATGGGGG41200.016.5603315
GTATATA23200.016.5362431
Produced by FastQC (version 0.11.5)