FastQCFastQC Report
Sat 18 Jun 2016
SRR1930932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518182
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.047.7169571
GTATCAA73100.038.91161
TCAACGC87200.032.121054
ATCAACG87450.031.9752923
CAACGCA88350.031.7443855
AACGCAG93850.030.1368566
TATCAAC97450.028.8892772
ACGCAGA109350.025.6914657
GTACATG119850.024.5653691
CGCAGAG114450.024.422248
TACATGG125500.022.7728082
ACATGGG122800.022.2294393
GCAGAGT133550.020.4319539
AGAGTAC125700.018.4800610-11
CATGGGG83550.018.2394944
GAGTACA93950.018.0443421
ACTACGC2453.800491E-617.4326088
CAGAGTA135050.016.7613810-11
GAGTACT78000.016.70066312-13
GTGGTAT24950.015.9874181
Produced by FastQC (version 0.11.5)