##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930932_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4518182 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32012079194685 31.0 31.0 34.0 30.0 34.0 2 31.453970867043427 31.0 31.0 34.0 30.0 34.0 3 31.540889012439074 31.0 31.0 34.0 30.0 34.0 4 35.39810813287291 37.0 35.0 37.0 33.0 37.0 5 35.19143119953999 37.0 35.0 37.0 32.0 37.0 6 35.22505180180878 37.0 35.0 37.0 32.0 37.0 7 35.11138196734881 37.0 35.0 37.0 32.0 37.0 8 35.088482048753235 37.0 35.0 37.0 32.0 37.0 9 36.60901707810797 39.0 35.0 39.0 32.0 39.0 10-11 36.543452432859056 38.0 35.0 39.0 32.0 39.0 12-13 36.453178291622606 38.0 35.0 39.0 32.0 39.0 14-15 37.503533943519756 39.0 36.0 41.0 32.0 41.0 16-17 37.474976771630715 39.0 36.0 41.0 32.0 41.0 18-19 37.45571659131925 39.0 36.0 41.0 32.0 41.0 20-21 37.42081461083241 39.0 36.0 41.0 32.0 41.0 22-23 37.29684406250125 39.0 36.0 40.0 31.5 41.0 24-25 37.190149157338055 39.0 36.0 40.0 31.0 41.0 26-27 36.94172800033287 39.0 36.0 40.0 31.0 41.0 28-29 36.767147051623866 39.0 36.0 40.0 30.0 41.0 30-31 36.55263068198669 38.0 35.0 40.0 30.0 41.0 32-33 36.34425516723319 38.0 35.0 40.0 30.0 41.0 34-35 36.556060490701796 38.0 35.0 40.0 30.0 41.0 36-37 36.61814608176475 39.0 35.0 40.0 30.0 41.0 38-39 36.59645749108823 39.0 35.0 40.0 30.0 41.0 40-41 36.52088218225826 39.0 35.0 40.0 30.0 41.0 42-43 36.40519295150129 38.5 35.0 40.0 30.0 41.0 44-45 36.25530666980657 38.0 35.0 40.0 30.0 41.0 46-47 36.07498502716358 38.0 35.0 40.0 29.0 41.0 48-49 35.91635474179659 38.0 35.0 40.0 29.0 41.0 50-51 35.6739278099023 38.0 34.0 40.0 28.0 41.0 52-53 35.483553783358 38.0 34.0 40.0 27.5 41.0 54-55 35.337909251995605 38.0 34.0 40.0 27.0 41.0 56-57 35.11389957288131 38.0 34.0 40.0 27.0 41.0 58-59 34.89989247887757 38.0 33.5 40.0 26.5 41.0 60-61 34.619804602824765 37.0 33.0 40.0 26.0 41.0 62-63 34.34204542889153 37.0 33.0 40.0 26.0 41.0 64-65 34.06606407178816 36.5 32.5 39.5 26.0 41.0 66-67 33.760661478444206 36.0 32.0 39.0 25.5 41.0 68-69 33.4273452242517 36.0 32.0 39.0 25.0 40.0 70-71 33.05415363967188 35.0 31.5 39.0 24.0 40.0 72-73 32.65927545636718 35.0 31.0 38.0 23.5 40.0 74-75 32.14855123144663 35.0 31.0 37.5 22.0 39.5 76-77 30.742792897674327 33.5 29.5 36.0 20.5 39.0 78-79 31.527962906319402 34.0 30.5 36.0 22.0 39.0 80-81 31.526217182043574 34.0 31.0 36.0 22.0 39.0 82-83 31.23720049347282 34.0 31.0 36.0 22.0 37.5 84-85 30.899085406475436 34.0 30.0 35.0 20.5 37.0 86-87 30.54576774463711 34.0 30.0 35.0 20.0 37.0 88-89 30.189923292156003 34.0 30.0 35.0 18.5 36.0 90-91 29.85064811466205 34.0 29.5 35.0 17.5 36.0 92-93 29.49816198196531 34.0 29.0 35.0 12.5 35.5 94-95 29.09397983525232 34.0 29.0 35.0 4.5 35.0 96-97 28.651623816836064 34.0 29.0 35.0 2.0 35.0 98-99 28.196840897511436 33.5 29.0 35.0 2.0 35.0 100-101 26.99933026159637 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45446215081043206 1101 2 0.5102874563865747 1101 3 0.46117503433674756 1101 4 0.29312550902596257 1101 5 0.4466768338243412 1101 6 0.41638513077799644 1101 7 0.43945780520544275 1101 8 0.37886281840976466 1101 9 0.5613805503761355 1101 10-11 0.5724495483120222 1101 12-13 0.5124951992192734 1101 14-15 0.6771666092077098 1101 16-17 0.675972275809329 1101 18-19 0.6697809193924584 1101 20-21 0.6739435656128379 1101 22-23 0.6964777153745629 1101 24-25 0.5614256536324405 1101 26-27 0.6862034773229979 1101 28-29 0.610564027957345 1101 30-31 0.6988016589732879 1101 32-33 0.5945437608292536 1101 34-35 0.6072168725386362 1101 36-37 0.6710750857478942 1101 38-39 0.6709606687045024 1101 40-41 0.79475915645817 1101 42-43 0.7419062097053342 1101 44-45 0.7245499490945235 1101 46-47 0.6628555046551128 1101 48-49 0.8666417259365318 1101 50-51 0.7273994080854536 1101 52-53 0.8243667951299045 1101 54-55 0.7132584795067203 1101 56-57 0.7651893571329964 1101 58-59 0.6429678636415872 1101 60-61 0.6322735776809765 1101 62-63 0.6494236299281653 1101 64-65 0.6944958822907523 1101 66-67 0.6378431916432277 1101 68-69 0.6492296367419996 1101 70-71 0.5874862423754692 1101 72-73 0.58999892894807 1101 74-75 0.5570941452943288 1101 76-77 0.7239516562434112 1101 78-79 0.7551404629343708 1101 80-81 0.6798577755193094 1101 82-83 0.7631637403592357 1101 84-85 0.7663634212842005 1101 86-87 0.7428669613454595 1101 88-89 0.7059584033414694 1101 90-91 0.6272639262269308 1101 92-93 0.8076234572519496 1101 94-95 0.7908553087431933 1101 96-97 0.6358311897144624 1101 98-99 0.8407747323471497 1101 100-101 0.9834116872296264 1102 1 0.45622760512279825 1102 2 0.46813910295919214 1102 3 0.49748171809109465 1102 4 0.28615357468013514 1102 5 0.39997311211742215 1102 6 0.39674720582190304 1102 7 0.4167933944798179 1102 8 0.46657255888136007 1102 9 0.55377815166338 1102 10-11 0.5782987167873088 1102 12-13 0.5853352770917724 1102 14-15 0.6533403977505188 1102 16-17 0.6320078254735932 1102 18-19 0.6819507820555017 1102 20-21 0.633667781238124 1102 22-23 0.6459772627756237 1102 24-25 0.6560973899687994 1102 26-27 0.666481781500714 1102 28-29 0.7300953723343895 1102 30-31 0.6463581177635689 1102 32-33 0.7508012082698343 1102 34-35 0.7742317883753671 1102 36-37 0.782704824066407 1102 38-39 0.7803137757972607 1102 40-41 0.8548122870129333 1102 42-43 0.790521894857541 1102 44-45 0.9185338963160987 1102 46-47 0.9934603097643233 1102 48-49 0.9127905362772424 1102 50-51 0.85980542549418 1102 52-53 0.8915977946031362 1102 54-55 0.879845039105561 1102 56-57 0.8582279981527989 1102 58-59 0.863157351183709 1102 60-61 0.8703790135113678 1102 62-63 0.6399744970773327 1102 64-65 0.6952002817661196 1102 66-67 0.606895455007745 1102 68-69 0.620256832946005 1102 70-71 0.5381380372394702 1102 72-73 0.5381007971976075 1102 74-75 0.7192184809826259 1102 76-77 0.8923708255240435 1102 78-79 1.0319606995924602 1102 80-81 0.8258217557931431 1102 82-83 0.9306682868933116 1102 84-85 0.9622648533294402 1102 86-87 0.8960716772086528 1102 88-89 0.9847449954419645 1102 90-91 0.9583528663359502 1102 92-93 0.9739781960018981 1102 94-95 0.9801892795089593 1102 96-97 0.9133499962996048 1102 98-99 0.9790965856648235 1102 100-101 1.277981974016697 1103 1 0.43511718014338285 1103 2 0.5834343313308104 1103 3 0.5104198936836184 1103 4 0.27427791777751764 1103 5 0.37972432691125135 1103 6 0.39315343239687905 1103 7 0.4648861320931701 1103 8 0.4304879610480654 1103 9 0.5865015727120024 1103 10-11 0.5423789208389991 1103 12-13 0.5958270791876004 1103 14-15 0.7539999760821772 1103 16-17 0.7136135700594366 1103 18-19 0.7301028212896696 1103 20-21 0.7180523341354714 1103 22-23 0.7239068329939542 1103 24-25 0.7367410553230656 1103 26-27 0.7990728399286695 1103 28-29 0.6957147879105321 1103 30-31 0.8001769517378676 1103 32-33 0.8423194931116313 1103 34-35 0.8166903959943497 1103 36-37 0.8489008658548371 1103 38-39 0.8957699227622626 1103 40-41 0.8970647544814696 1103 42-43 0.8832314733495394 1103 44-45 0.8512606544901544 1103 46-47 0.851592668977716 1103 48-49 0.8858651952891563 1103 50-51 0.8534696723941622 1103 52-53 0.7314228139845156 1103 54-55 0.7294316263867202 1103 56-57 0.8978057796318097 1103 58-59 0.853256611123534 1103 60-61 0.7999604307534582 1103 62-63 0.8004902052173577 1103 64-65 0.716420917066614 1103 66-67 0.6380274530499577 1103 68-69 0.6847169682268586 1103 70-71 0.6630584422883246 1103 72-73 0.6246802115631667 1103 74-75 0.8822383860839977 1103 76-77 0.9520315881844752 1103 78-79 0.9499217299845526 1103 80-81 0.9266240889348722 1103 82-83 0.8401484088497178 1103 84-85 0.9293480193054293 1103 86-87 0.9581841748135389 1103 88-89 1.0236585085752061 1103 90-91 1.0052539846036765 1103 92-93 0.7991946732240613 1103 94-95 0.8409649076301271 1103 96-97 0.7803927560001185 1103 98-99 0.9123889264709142 1103 100-101 1.021186539404713 1104 1 0.3785417506744153 1104 2 0.46969798223695136 1104 3 0.4461087441303597 1104 4 0.22034393374230632 1104 5 0.3337407234277947 1104 6 0.2884370111984822 1104 7 0.35860099010699287 1104 8 0.45797316625292694 1104 9 0.5408385879128446 1104 10-11 0.534769931788702 1104 12-13 0.5052302763913445 1104 14-15 0.5638913657807549 1104 16-17 0.5708340785442942 1104 18-19 0.6287575178069957 1104 20-21 0.5595372290238103 1104 22-23 0.522843775049445 1104 24-25 0.6379247881708636 1104 26-27 0.5773809062470576 1104 28-29 0.5572078051388729 1104 30-31 0.6046082678260589 1104 32-33 0.6989158061219314 1104 34-35 0.674313892317997 1104 36-37 0.7135634828090431 1104 38-39 0.6377137885926842 1104 40-41 0.6317121622673696 1104 42-43 0.5390610599935854 1104 44-45 0.5908314606473297 1104 46-47 0.7329328733716451 1104 48-49 0.6458800881714666 1104 50-51 0.6306920160117997 1104 52-53 0.602590620363955 1104 54-55 0.6537864137797058 1104 56-57 0.7400091058918008 1104 58-59 0.6709020626059115 1104 60-61 0.7305150254960182 1104 62-63 0.5899190942798782 1104 64-65 0.6226244023842753 1104 66-67 0.5797810754995112 1104 68-69 0.5845426450560325 1104 70-71 0.5115276974839063 1104 72-73 0.5264480144090555 1104 74-75 0.7701331725112794 1104 76-77 0.847642934600497 1104 78-79 0.8547936368025617 1104 80-81 0.737494328439432 1104 82-83 0.8850635722311715 1104 84-85 0.8403584981927636 1104 86-87 0.8442428175114074 1104 88-89 0.904789348005469 1104 90-91 0.8657591496695822 1104 92-93 0.8546840189352025 1104 94-95 0.957403552310435 1104 96-97 0.9150250908565845 1104 98-99 0.9942448022102823 1104 100-101 1.2277646719876607 1105 1 0.1974511300340822 1105 2 0.3122641707208089 1105 3 0.31683265791108894 1105 4 0.19529812510823064 1105 5 0.2393244940699617 1105 6 0.2975644782101483 1105 7 0.30014157769954153 1105 8 0.27439450826295086 1105 9 0.4378801708634654 1105 10-11 0.30494402652998787 1105 12-13 0.29988124541969796 1105 14-15 0.48430990790611617 1105 16-17 0.43117921142453497 1105 18-19 0.4354177018910974 1105 20-21 0.40607984011479914 1105 22-23 0.4284072760780404 1105 24-25 0.46900715552321515 1105 26-27 0.4674886422443407 1105 28-29 0.6383344401267692 1105 30-31 0.6351398696080253 1105 32-33 0.5820289094113136 1105 34-35 0.5998246784996653 1105 36-37 0.5780677187658156 1105 38-39 0.5043742681791272 1105 40-41 0.5253042171329199 1105 42-43 0.44193539739307397 1105 44-45 0.4177780419045831 1105 46-47 0.5083929278040955 1105 48-49 0.4890197127957663 1105 50-51 0.48511595151656906 1105 52-53 0.4617894350301981 1105 54-55 0.5557620316046865 1105 56-57 0.5781893932539646 1105 58-59 0.4720315279521472 1105 60-61 0.4870675705445393 1105 62-63 0.46408011581299036 1105 64-65 0.43722990560744535 1105 66-67 0.49039538099859925 1105 68-69 0.5337846469273941 1105 70-71 0.49720855449609047 1105 72-73 0.4914555739591222 1105 74-75 0.5595331111483688 1105 76-77 0.6034196936647405 1105 78-79 0.5495585358342652 1105 80-81 0.5771682416083763 1105 82-83 0.6235624480525459 1105 84-85 0.6369951044222759 1105 86-87 0.700049980995999 1105 88-89 0.6437089683680739 1105 90-91 0.6233215277892974 1105 92-93 0.6089878604425216 1105 94-95 0.4716789009281648 1105 96-97 0.25077579492553426 1105 98-99 0.5453203268365918 1105 100-101 0.9706907038275787 1106 1 0.11678860762037147 1106 2 0.15507514083342144 1106 3 0.137626071751086 1106 4 -0.0025553405382510164 1106 5 0.07329992751244419 1106 6 0.08449845517160526 1106 7 0.11095171338097032 1106 8 0.08235947278301126 1106 9 0.13355753831281447 1106 10-11 0.15057060396034672 1106 12-13 0.15091421940284988 1106 14-15 0.20062064582629802 1106 16-17 0.10331585351667627 1106 18-19 0.14791071784826926 1106 20-21 0.12843356054181498 1106 22-23 0.18575641577019297 1106 24-25 0.2570120079796965 1106 26-27 0.23919371865525108 1106 28-29 0.08816944730513399 1106 30-31 0.22273228983713977 1106 32-33 0.2244429932277967 1106 34-35 0.10189514408701683 1106 36-37 0.23219493098837063 1106 38-39 0.24792712467756672 1106 40-41 0.31094236764543837 1106 42-43 0.36167268444205547 1106 44-45 0.25789571264574107 1106 46-47 0.20677980491218761 1106 48-49 0.22991952989855236 1106 50-51 0.13291344516698445 1106 52-53 0.0468641451882732 1106 54-55 0.2536322390232755 1106 56-57 0.2886254300513258 1106 58-59 0.2805677722278901 1106 60-61 0.03606960812450666 1106 62-63 0.08374027112614613 1106 64-65 0.12510520863683894 1106 66-67 0.08376876171379877 1106 68-69 0.17501192512050068 1106 70-71 0.13467139208031398 1106 72-73 0.1149590289426925 1106 74-75 0.314155335053627 1106 76-77 0.40045267960630326 1106 78-79 0.287631850180297 1106 80-81 0.3068554561428911 1106 82-83 0.30115949884323 1106 84-85 0.3082310790359024 1106 86-87 0.31198081887518825 1106 88-89 0.31579618237110196 1106 90-91 0.3242111669576033 1106 92-93 0.4385650576174136 1106 94-95 0.41500454766216066 1106 96-97 0.3950207111070547 1106 98-99 0.46400607115119996 1106 100-101 0.6737587000035425 1107 1 0.1817737506773085 1107 2 0.2024026514035917 1107 3 0.19200961137159567 1107 4 0.0331249527539228 1107 5 0.1353258654648286 1107 6 0.10306195969320697 1107 7 0.22185225068670178 1107 8 0.13224179136449976 1107 9 0.16309664962949455 1107 10-11 0.15464505534259843 1107 12-13 0.10584687972091444 1107 14-15 0.1313276569888231 1107 16-17 0.17056056045430523 1107 18-19 0.20269319149416987 1107 20-21 0.21696062379945857 1107 22-23 0.24367967648981903 1107 24-25 0.17552345726916485 1107 26-27 0.09146458154769022 1107 28-29 0.31901207262313847 1107 30-31 0.2584122362678585 1107 32-33 0.36112168818240065 1107 34-35 0.17331454536599722 1107 36-37 0.19352664309128187 1107 38-39 0.22362553454326672 1107 40-41 0.2162437069988954 1107 42-43 0.32766560742788897 1107 44-45 0.2985937687127702 1107 46-47 0.2363164014833501 1107 48-49 0.2542895818115696 1107 50-51 0.19417919289360697 1107 52-53 0.08736383986347818 1107 54-55 0.23158351400792299 1107 56-57 0.13103346103854108 1107 58-59 0.19222933656262597 1107 60-61 0.23070852622201699 1107 62-63 0.04463246641400076 1107 64-65 0.10291114867386142 1107 66-67 0.13329277241724924 1107 68-69 0.19613459510038922 1107 70-71 0.26798050147711905 1107 72-73 0.2835360350462395 1107 74-75 0.4661910822478177 1107 76-77 0.4682834956793762 1107 78-79 0.4753160227709259 1107 80-81 0.39689858576276293 1107 82-83 0.43002394729623816 1107 84-85 0.4787480161937978 1107 86-87 0.4497478566662636 1107 88-89 0.5063161641053817 1107 90-91 0.41083159786011336 1107 92-93 0.4590559194657935 1107 94-95 0.544120037089737 1107 96-97 0.5692732160103766 1107 98-99 0.4394553044226335 1107 100-101 0.6654106337301151 1108 1 0.24385207615825522 1108 2 0.29432942260917727 1108 3 0.28754106970608007 1108 4 0.12594978884801833 1108 5 0.19723400221234044 1108 6 0.13082895923476912 1108 7 0.2856012219596735 1108 8 0.2864250166239657 1108 9 0.2728150476874802 1108 10-11 0.30485542656807496 1108 12-13 0.27707297044773327 1108 14-15 0.36541259393958825 1108 16-17 0.4232084106219389 1108 18-19 0.4208436473099937 1108 20-21 0.45024260133040883 1108 22-23 0.3938180713442492 1108 24-25 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-0.8275068088310036 2316 68-69 -0.9502971101400917 2316 70-71 -0.8495208451311669 2316 72-73 -0.7832915730299348 2316 74-75 -0.5760305173490678 2316 76-77 -0.9460798192358304 2316 78-79 -0.7185238959113107 2316 80-81 -0.7840280110540263 2316 82-83 -1.0165428531624805 2316 84-85 -1.0653566630527962 2316 86-87 -0.9296427379264536 2316 88-89 -0.9411824024083799 2316 90-91 -0.7805659643445004 2316 92-93 -0.9976712576957283 2316 94-95 -0.8022764825794333 2316 96-97 -0.7582284346707517 2316 98-99 -0.7815068254772832 2316 100-101 -0.8035565611545117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 19.0 4 72.0 5 141.0 6 242.0 7 697.0 8 1715.0 9 2985.0 10 4598.0 11 7102.0 12 9773.0 13 12340.0 14 14661.0 15 17362.0 16 20210.0 17 22844.0 18 21292.0 19 19723.0 20 20326.0 21 22263.0 22 25626.0 23 29588.0 24 36713.0 25 47246.0 26 60701.0 27 78742.0 28 101300.0 29 127413.0 30 157284.0 31 192120.0 32 231928.0 33 283566.0 34 350512.0 35 439533.0 36 568657.0 37 736524.0 38 699528.0 39 152809.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.499474357756824 22.305361285236792 14.750125682620999 23.445038674385383 2 14.710123547729301 22.28750660412292 40.93179498677626 22.07057486137152 3 17.539135082851836 27.784564204776224 31.444808051341244 23.231492661030696 4 12.352623386745016 18.430329747215204 40.24213493221506 28.974911933824714 5 12.203299347115806 38.869694513586 36.56277652627008 12.364229613028115 6 28.816890871988278 40.0617726606731 18.58257085817741 12.538765609161212 7 24.912475858652883 33.36109523697806 24.833838034855614 16.892590869513448 8 23.696682359560185 37.617682868393274 21.74432586919382 16.941308902852718 9 24.930049298589566 16.49838364191615 22.09187235042767 36.479694709066614 10-11 23.369178134037096 27.314526063801768 30.345900187287718 18.970395614873418 12-13 23.698359207309487 24.949780686125525 30.2408468715957 21.111013234969285 14-15 21.141605438117686 26.75944146549242 27.2851397498241 24.813813346565798 16-17 21.02304643770437 29.73208029247162 28.041842935941936 21.20303033388208 18-19 20.98815408498374 28.92348072742532 29.580094383094796 20.508270804496146 20-21 21.640695306209444 28.16054112030016 29.379748314698258 20.81901525879214 22-23 21.428960333585646 27.998541448105215 29.18356506380888 21.388933154500265 24-25 21.31615996692458 28.172105054293134 29.161589930766045 21.350145048016238 26-27 21.33560154768889 28.22336599424779 29.071666653940053 21.369365804123266 28-29 21.423319626477667 28.33677166697985 28.995256272607296 21.24465243393519 30-31 21.664460987590868 28.079711227581587 28.930209126708238 21.3256186581193 32-33 21.46004257305421 28.254463822699865 28.86497793477851 21.42051566946742 34-35 21.648639498209164 28.148801808579126 28.919911647376654 21.282647045835056 36-37 21.716268512852068 28.021378242619484 28.90302325401243 21.359329990516017 38-39 21.59524778368642 28.064662385189322 28.962663766065916 21.377426065058344 40-41 21.584518728481587 28.038589856285146 29.053733794133947 21.323157621099323 42-43 21.603330025309866 28.087554051432296 28.727511057087806 21.581604866170032 44-45 21.547331072866573 28.218688814917265 28.721821037392438 21.51215907482372 46-47 21.694312332012714 28.14113953455942 28.549411033224604 21.61513710020326 48-49 21.615957460113044 28.120409412988117 28.54111788060238 21.72251524629646 50-51 21.627473175249417 28.200762372474042 28.38918209959366 21.782582352682876 52-53 21.76804985910169 28.174790302281156 28.3852160132582 21.671943825358948 54-55 21.598880495210192 28.11579210796939 28.4962692212887 21.78905817553172 56-57 21.66056583354897 28.207253752969713 28.33502925942888 21.79715115405244 58-59 21.67407510550924 28.074738251087467 28.44501814540833 21.806168497994967 60-61 21.70008246914936 28.249769467281183 28.414364513640184 21.63578354992927 62-63 21.65878029394578 28.182371561424258 28.369617294447046 21.78923085018292 64-65 21.476562781281245 28.073093657480108 28.365003004949163 22.085340556289484 66-67 21.493540017327067 28.04400233095585 28.385790918578298 22.07666673313879 68-69 21.489406251557906 28.12900831436152 28.385795237383054 21.99579019669752 70-71 21.512277530578764 27.843270224198285 28.498949165718557 22.145503079504394 72-73 21.584521567622073 27.94921956111145 28.34003425304172 22.126224618224764 74-75 21.4675638454367 28.139521957074283 28.24070586426201 22.15220833322701 76-77 21.425620238371167 28.005404246136305 28.35726191966851 22.21171359582402 78-79 21.309101412833925 28.103652827549265 28.25974408294174 22.32750167667507 80-81 21.419436830698622 28.08832564876197 28.171069802113436 22.321167718425976 82-83 21.50027378268516 27.91502201549207 28.29800791557312 22.286696286249647 84-85 21.48624003470424 27.889138989593153 28.137026413290307 22.4875945624123 86-87 21.26129491906258 28.04428860988778 28.273097453798897 22.42131901725074 88-89 21.388450904480838 27.818307935156078 28.158069236850658 22.635171923512427 90-91 21.361816123735746 27.814590717871145 28.215331241204407 22.608261917188702 92-93 21.39057527878469 27.73256592600791 28.22953595838291 22.647322836824483 94-95 21.21594991529082 27.60107084706189 28.30931017436401 22.873669063283273 96-97 21.193225505302795 27.486619618244685 28.30665077236818 23.013504104084344 98-99 21.009722494578572 27.57090130499391 28.25078759554175 23.16858860488577 100-101 21.042147040557463 27.44649285929606 28.230392224129087 23.28096787601739 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170.0 1 125.5 2 131.0 3 195.0 4 165.0 5 148.5 6 230.5 7 431.5 8 690.5 9 818.0 10 745.5 11 692.0 12 799.5 13 953.5 14 1145.5 15 1334.0 16 1524.5 17 1761.0 18 2054.0 19 2373.5 20 2806.5 21 3371.0 22 4103.0 23 5213.5 24 6959.0 25 10013.5 26 15319.5 27 24038.5 28 37604.5 29 58031.0 30 83138.0 31 109651.0 32 135440.5 33 153871.5 34 168143.0 35 177376.0 36 185182.5 37 191052.5 38 183142.5 39 168390.5 40 154896.0 41 144943.5 42 139095.5 43 136920.5 44 136336.5 45 137434.5 46 138523.0 47 139046.0 48 139672.0 49 138945.0 50 135491.0 51 131123.0 52 128673.0 53 126237.0 54 121905.0 55 112961.5 56 101398.5 57 90043.5 58 79556.0 59 69627.0 60 58541.5 61 48127.5 62 39846.5 63 31285.0 64 23143.5 65 17337.0 66 13079.5 67 10243.5 68 8025.5 69 6188.0 70 4883.0 71 3824.5 72 2913.5 73 2250.5 74 1818.0 75 1475.5 76 1095.0 77 721.0 78 455.5 79 307.5 80 208.0 81 131.0 82 78.5 83 48.5 84 27.5 85 14.0 86 9.5 87 8.5 88 7.0 89 4.5 90 1.0 91 0.0 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01883500930241411 2 0.003784708097194845 3 0.003917504872535015 4 0.03744869064592794 5 0.0016820924876421533 6 4.426559178005667E-5 7 0.0 8 2.2132795890028335E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 2.2132795890028335E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.426559178005667E-5 24-25 5.090543054706517E-4 26-27 0.0020030180280475643 28-29 1.1066397945014167E-4 30-31 0.006617705971118471 32-33 0.02225452626742349 34-35 0.013335009523742072 36-37 8.189134479310484E-4 38-39 8.299798458760626E-4 40-41 0.0022907443746179325 42-43 0.008377263244375724 44-45 0.008487927223825867 46-47 0.007857142540960059 48-49 0.009860160569007624 50-51 0.20140844259925786 52-53 0.0517132775970512 54-55 0.01831488859899845 56-57 0.01091146837378397 58-59 0.020439637004441166 60-61 0.056527160703132365 62-63 0.10168913071673519 64-65 0.10611568989474085 66-67 0.110929573000822 68-69 0.10860562943236904 70-71 0.09710764196749933 72-73 0.07203118422409721 74-75 0.2553349997853119 76-77 0.30866397148233515 78-79 0.00690543231768884 80-81 8.410462438210766E-4 82-83 0.0 84-85 4.426559178005667E-5 86-87 0.0 88-89 2.2132795890028334E-4 90-91 0.006551307583448387 92-93 0.017861166283252867 94-95 0.0016931588855871676 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4518182.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.46273828019509 #Duplication Level Percentage of deduplicated Percentage of total 1 74.81330080164177 39.99723920635683 2 13.58579496316232 14.526676008878797 3 4.676562777409476 7.500655554586352 4 2.1649623261762634 4.629792569233757 5 1.2442384848865577 3.326019823781825 6 0.729139857282097 2.3389088015718924 7 0.5233634547508904 1.9586310384735905 8 0.37976346872276134 1.6242555949363233 9 0.2679499169197655 1.2892802652433293 >10 1.4778574976509384 14.105092634001004 >50 0.09252836314195378 3.3817134340676325 >100 0.04172531084999287 3.9979819010510833 >500 0.0023164051963890883 0.8359290909743519 >1k 4.96372208655966E-4 0.4878240768432288 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2132795890028335E-5 2 0.0 0.0 0.0 0.0 4.426559178005667E-5 3 0.0 0.0 0.0 0.0 4.426559178005667E-5 4 0.0 0.0 0.0 0.0 4.426559178005667E-5 5 0.0 2.2132795890028335E-5 0.0 0.0 4.426559178005667E-5 6 0.0 2.2132795890028335E-5 0.0 0.0 6.639838767008501E-5 7 0.0 2.2132795890028335E-5 0.0 0.0 6.639838767008501E-5 8 0.0 2.2132795890028335E-5 0.0 0.0 6.639838767008501E-5 9 0.0 2.2132795890028335E-5 0.0 0.0 8.853118356011334E-5 10-11 0.0 2.2132795890028335E-5 0.0 0.0 8.853118356011334E-5 12-13 0.0 2.2132795890028335E-5 0.0 2.2132795890028335E-5 2.2132795890028334E-4 14-15 0.0 3.3199193835042504E-5 0.0 2.2132795890028335E-5 2.7665994862535417E-4 16-17 0.0 4.426559178005667E-5 0.0 2.2132795890028335E-5 3.0985914246039667E-4 18-19 0.0 4.426559178005667E-5 0.0 4.426559178005667E-5 3.0985914246039667E-4 20-21 0.0 4.426559178005667E-5 0.0 4.426559178005667E-5 4.426559178005667E-4 22-23 0.0 4.426559178005667E-5 0.0 6.639838767008501E-5 5.533198972507084E-4 24-25 0.0 4.426559178005667E-5 0.0 1.5492957123019834E-4 5.975854890307651E-4 26-27 0.0 4.426559178005667E-5 0.0 3.4305833629543923E-4 6.529174787558358E-4 28-29 2.2132795890028335E-5 4.426559178005667E-5 0.0 0.0014164989369618135 6.750502746458642E-4 30-31 2.2132795890028335E-5 4.426559178005667E-5 0.0 0.00571026133962731 6.971830705358925E-4 32-33 3.3199193835042504E-5 4.426559178005667E-5 0.0 0.0185915485476238 7.193158664259209E-4 34-35 4.426559178005667E-5 5.5331989725070836E-5 0.0 0.03776961618633335 7.525150602609634E-4 36-37 4.426559178005667E-5 6.639838767008501E-5 0.0 0.06935311592140378 7.746478561509917E-4 38-39 4.426559178005667E-5 6.639838767008501E-5 0.0 0.11704929106441485 7.9678065204102E-4 40-41 4.426559178005667E-5 6.639838767008501E-5 0.0 0.17106437943402902 7.9678065204102E-4 42-43 4.426559178005667E-5 6.639838767008501E-5 0.0 0.22825552401386223 8.189134479310484E-4 44-45 4.426559178005667E-5 6.639838767008501E-5 0.0 0.299157935647568 9.074446314911618E-4 46-47 4.426559178005667E-5 6.639838767008501E-5 0.0 0.3811599444201229 9.84909417106261E-4 48-49 4.426559178005667E-5 6.639838767008501E-5 0.0 0.4700231199185867 0.0010291750088863177 50-51 4.426559178005667E-5 6.639838767008501E-5 0.0 0.5672082266717011 0.0011066397945014167 52-53 4.426559178005667E-5 6.639838767008501E-5 0.0 0.6610955468372013 0.0012062373760065442 54-55 4.426559178005667E-5 7.746478561509917E-5 0.0 0.7688158644339692 0.001261569365731615 56-57 4.426559178005667E-5 8.853118356011334E-5 0.0 0.89441948110988 0.0012726357636766291 58-59 4.426559178005667E-5 8.853118356011334E-5 0.0 1.0345975438793744 0.0013169013554566859 60-61 4.426559178005667E-5 1.1066397945014167E-4 0.0 1.1848570951767767 0.0013279677534017 62-63 4.426559178005667E-5 1.1066397945014167E-4 0.0 1.3434385777288298 0.0013722333451817567 64-65 4.426559178005667E-5 1.1066397945014167E-4 0.0 1.5065572834383387 0.0013832997431267708 66-67 4.426559178005667E-5 1.1066397945014167E-4 0.0 1.695825887491916 0.0014164989369618135 68-69 4.426559178005667E-5 1.1066397945014167E-4 0.0 1.9065743699567657 0.00146076452874187 70-71 4.426559178005667E-5 1.2173037739515585E-4 0.0 2.1363238576932053 0.0014828973246318984 72-73 4.426559178005667E-5 1.3279677534017002E-4 0.0 2.389025054767603 0.0015714285081920116 74-75 4.426559178005667E-5 1.3279677534017002E-4 0.0 2.646186010213843 0.0016156940999720684 76-77 4.426559178005667E-5 1.3279677534017002E-4 0.0 2.931499882032198 0.0016156940999720684 78-79 4.426559178005667E-5 1.3279677534017002E-4 0.0 3.2496698893493 0.0016599596917521251 80-81 4.426559178005667E-5 1.3279677534017002E-4 0.0 3.6075461324931135 0.001715291681477196 82-83 4.426559178005667E-5 1.3279677534017002E-4 0.0 3.982276499707183 0.001848088456817366 84-85 4.426559178005667E-5 1.3279677534017002E-4 0.0 4.370806665158685 0.0018812876506524084 86-87 4.426559178005667E-5 1.3279677534017002E-4 0.0 4.787677875747369 0.0020030180280475643 88-89 4.426559178005667E-5 1.3279677534017002E-4 0.0 5.245074235610694 0.0021358148033877346 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4120 0.0 47.716957 1 GTATCAA 7310 0.0 38.9116 1 TCAACGC 8720 0.0 32.12105 4 ATCAACG 8745 0.0 31.975292 3 CAACGCA 8835 0.0 31.744385 5 AACGCAG 9385 0.0 30.136856 6 TATCAAC 9745 0.0 28.889277 2 ACGCAGA 10935 0.0 25.691465 7 GTACATG 11985 0.0 24.565369 1 CGCAGAG 11445 0.0 24.42224 8 TACATGG 12550 0.0 22.772808 2 ACATGGG 12280 0.0 22.229439 3 GCAGAGT 13355 0.0 20.431953 9 AGAGTAC 12570 0.0 18.48006 10-11 CATGGGG 8355 0.0 18.239494 4 GAGTACA 9395 0.0 18.044342 1 ACTACGC 245 3.800491E-6 17.432608 8 CAGAGTA 13505 0.0 16.76138 10-11 GAGTACT 7800 0.0 16.700663 12-13 GTGGTAT 2495 0.0 15.987418 1 >>END_MODULE