FastQCFastQC Report
Sat 18 Jun 2016
SRR1930931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477194
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62840.18072043147434397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25250.050.087211
GTATCAA46300.046.4687961
ATCAACG57950.036.6759033
TCAACGC60000.035.91264
CAACGCA60300.034.705945
TATCAAC62600.034.1184042
AACGCAG65750.032.221956
GTACATG83400.028.0932181
ACGCAGA77700.027.050167
TACATGG86000.026.885092
CGCAGAG81450.026.0913688
ACATGGG88150.025.7105873
GCAGAGT90650.022.3880589
CATGGGG69050.021.7273564
AGAGTAC83500.019.18134110-11
GTGGTAT13300.018.9423081
CAGAGTA88450.018.08286910-11
ATGGGGG44200.017.9330775
GAGTACA63800.017.92751
GTATTAC5800.017.374671
Produced by FastQC (version 0.11.5)