##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930931_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3477194 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.824145561047214 30.0 16.0 31.0 2.0 33.0 2 22.580282837253257 30.0 10.0 31.0 2.0 34.0 3 23.093575739518705 30.0 16.0 31.0 2.0 34.0 4 25.074606133566316 33.0 10.0 35.0 2.0 37.0 5 25.0537830215973 33.0 10.0 35.0 2.0 37.0 6 25.88940392741964 33.0 17.0 36.0 2.0 37.0 7 26.034055908298473 33.0 17.0 36.0 2.0 37.0 8 26.931414525620372 33.0 17.0 36.0 2.0 37.0 9 28.249796243752865 35.0 19.0 38.0 2.0 39.0 10-11 28.60412562543246 35.0 25.5 38.0 2.0 39.0 12-13 28.597132630506092 35.0 25.5 38.0 2.0 39.0 14-15 28.897446044137887 36.0 25.0 39.0 2.0 40.0 16-17 27.976968354368495 34.5 18.0 38.5 2.0 40.0 18-19 27.304976512670848 34.0 17.0 38.5 2.0 40.0 20-21 26.766239962452484 34.0 13.0 38.5 2.0 40.0 22-23 26.749162686925146 34.0 16.0 38.0 2.0 40.0 24-25 26.46915170680727 34.0 12.5 38.0 2.0 40.0 26-27 26.173906891591322 33.0 10.0 38.0 2.0 40.0 28-29 26.175069466932243 33.0 10.5 38.0 2.0 40.0 30-31 26.176941234800246 33.0 10.0 38.0 2.0 40.0 32-33 25.95398473596814 33.0 9.0 38.0 2.0 40.0 34-35 22.84251108796346 30.0 5.0 37.0 2.0 40.0 36-37 21.125350066749224 22.5 5.0 36.0 2.0 39.0 38-39 20.14660643035735 20.5 2.0 35.0 2.0 39.0 40-41 21.712338598306566 26.5 2.0 35.5 2.0 39.0 42-43 21.665330723566186 26.5 2.0 36.0 2.0 39.0 44-45 19.544991306208395 20.5 2.0 35.0 2.0 38.5 46-47 20.552664015870267 22.5 2.0 34.5 2.0 38.0 48-49 20.012290513557772 24.0 2.0 35.0 2.0 38.5 50-51 17.741915176432492 12.0 2.0 33.5 2.0 37.5 52-53 20.961763134297367 24.5 2.0 34.5 2.0 38.0 54-55 22.878893441090717 30.0 2.0 36.5 2.0 39.0 56-57 23.162218587746327 31.0 2.0 37.0 2.0 39.0 58-59 22.772541595320824 30.0 2.0 37.0 2.0 39.0 60-61 22.478382856981806 29.5 2.0 36.0 2.0 39.0 62-63 22.008115451711927 29.0 2.0 36.0 2.0 39.0 64-65 21.51192254444244 28.0 2.0 35.0 2.0 39.0 66-67 21.100556943328442 28.0 2.0 35.0 2.0 38.0 68-69 20.645747691960818 27.5 2.0 34.5 2.0 38.0 70-71 20.04464087997391 26.0 2.0 34.0 2.0 37.0 72-73 19.57933494651147 26.0 2.0 34.0 2.0 36.5 74-75 19.040453739423224 25.0 2.0 34.0 2.0 36.0 76-77 18.47124060377419 23.5 2.0 33.0 2.0 35.5 78-79 17.98543236299154 22.0 2.0 32.5 2.0 35.0 80-81 17.48558823580163 20.0 2.0 32.0 2.0 35.0 82-83 17.010899449383615 18.5 2.0 32.0 2.0 35.0 84-85 16.489303012716576 16.5 2.0 31.5 2.0 34.0 86-87 15.912936695507929 8.5 2.0 31.0 2.0 34.0 88-89 15.34679902818192 4.5 2.0 31.0 2.0 34.0 90-91 14.746504796683762 2.0 2.0 31.0 2.0 34.0 92-93 14.085377174813946 2.0 2.0 30.0 2.0 34.0 94-95 13.391929670878302 2.0 2.0 29.5 2.0 34.0 96-97 12.610146140825044 2.0 2.0 29.0 2.0 34.0 98-99 11.725188470933748 2.0 2.0 29.0 2.0 34.0 100-101 10.474192696754912 2.0 2.0 25.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.556260448464549 1101 2 7.895257954276037 1101 3 7.4204648228392145 1101 4 9.184465926549546 1101 5 9.13156148769431 1101 6 8.518022180892675 1101 7 8.178351582153514 1101 8 7.203927924553337 1101 9 7.339223660749351 1101 10-11 6.723914575691776 1101 12-13 6.465031966720456 1101 14-15 6.977041157360482 1101 16-17 7.735618020912181 1101 18-19 8.259289926125028 1101 20-21 8.728884816533384 1101 22-23 8.604998815367324 1101 24-25 8.665697077523639 1101 26-27 8.703561868334276 1101 28-29 8.444677077125807 1101 30-31 8.349531679687622 1101 32-33 8.144070943063582 1101 34-35 11.005395655854699 1101 36-37 4.864490362679621 1101 38-39 8.311233770707268 1101 40-41 9.844737763253843 1101 42-43 10.494773943774337 1101 44-45 4.322665050799365 1101 46-47 6.025265768612417 1101 48-49 9.321043781030479 1101 50-51 -4.71042763098175 1101 52-53 5.435926693401679 1101 54-55 8.02144356743511 1101 56-57 8.466016763198603 1101 58-59 8.788638372596697 1101 60-61 8.633338964803542 1101 62-63 8.659521853443525 1101 64-65 8.749163520417813 1101 66-67 8.55536852214967 1101 68-69 8.611327909220282 1101 70-71 8.701529537775773 1101 72-73 8.617564984481636 1101 74-75 8.567415904810776 1101 76-77 8.477417382944683 1101 78-79 8.465704267279076 1101 80-81 8.292185560087734 1101 82-83 8.150634546917686 1101 84-85 8.125632080900434 1101 86-87 8.010824085173786 1101 88-89 7.937888069795003 1101 90-91 7.9253289151973085 1101 92-93 7.86796592441649 1101 94-95 7.668982789479246 1101 96-97 7.611217961543781 1101 98-99 7.19067616919301 1101 100-101 6.828457514820617 1102 1 6.55495134782624 1102 2 7.708228561622288 1102 3 7.317845057938747 1102 4 9.212067362303717 1102 5 9.103593469014218 1102 6 8.413361018181977 1102 7 7.979190205747553 1102 8 7.157045269311549 1102 9 6.982883339754746 1102 10-11 6.46082487232859 1102 12-13 6.209737362612639 1102 14-15 6.8011908065875275 1102 16-17 7.473254099621524 1102 18-19 7.989877970309564 1102 20-21 8.337874176941735 1102 22-23 8.038838415357603 1102 24-25 8.084898552194673 1102 26-27 8.165091509430482 1102 28-29 8.00066871000082 1102 30-31 7.849838323699117 1102 32-33 7.865101770776562 1102 34-35 10.477642635454629 1102 36-37 0.5339672088850556 1102 38-39 3.0703753238374674 1102 40-41 8.2238249022739 1102 42-43 9.371319759690639 1102 44-45 0.45426571516576786 1102 46-47 0.979061552387563 1102 48-49 7.485476637284801 1102 50-51 -5.952927014219007 1102 52-53 4.360122643268429 1102 54-55 7.187812711229434 1102 56-57 7.630141238081592 1102 58-59 8.04148807707179 1102 60-61 7.940848615837446 1102 62-63 7.998551121007367 1102 64-65 8.036245728810652 1102 66-67 7.992901749156584 1102 68-69 7.856617492358165 1102 70-71 7.96419749703816 1102 72-73 7.970454040157069 1102 74-75 7.778438671174097 1102 76-77 7.802975162320838 1102 78-79 7.669206002901557 1102 80-81 7.652190407321687 1102 82-83 7.512262088240792 1102 84-85 7.401191250164455 1102 86-87 7.382353708896394 1102 88-89 7.3774679066916615 1102 90-91 7.291031868361564 1102 92-93 7.154185533649516 1102 94-95 6.911647221901161 1102 96-97 6.954762192987909 1102 98-99 6.663674991263038 1102 100-101 6.109962363791926 1103 1 6.441222796850354 1103 2 7.766938904051852 1103 3 7.3538550413945245 1103 4 8.969342737642304 1103 5 9.055765872950676 1103 6 8.30333016945212 1103 7 7.863331137297525 1103 8 7.016227870776401 1103 9 7.158012680448213 1103 10-11 6.614064978894685 1103 12-13 6.41167806078634 1103 14-15 6.891737489885045 1103 16-17 7.669844838568022 1103 18-19 8.144267479761552 1103 20-21 8.421536817063402 1103 22-23 8.32343434401821 1103 24-25 8.481950815610404 1103 26-27 8.430919116630747 1103 28-29 8.294182526346322 1103 30-31 8.089081793556332 1103 32-33 8.076366228411828 1103 34-35 10.667433784727578 1103 36-37 -3.568351148027201 1103 38-39 -1.9747104722589839 1103 40-41 7.1074668466471955 1103 42-43 9.510339659781838 1103 44-45 0.6865108357331984 1103 46-47 0.2850410768988745 1103 48-49 7.804515828659529 1103 50-51 -6.076935226935415 1103 52-53 4.32626390397051 1103 54-55 7.472317380610988 1103 56-57 8.108857099377385 1103 58-59 8.364054980950709 1103 60-61 8.413215924845694 1103 62-63 8.389639203844805 1103 64-65 8.340838730987262 1103 66-67 8.119544416179625 1103 68-69 8.006709927180477 1103 70-71 8.207183680073957 1103 72-73 8.195224716416167 1103 74-75 8.117803933499669 1103 76-77 7.933706709075903 1103 78-79 7.623329653965673 1103 80-81 7.505658940503562 1103 82-83 7.412274864539857 1103 84-85 7.398771951208953 1103 86-87 7.1637600484155595 1103 88-89 7.160818068854761 1103 90-91 7.196412391713416 1103 92-93 7.123240594858197 1103 94-95 6.7853680452269085 1103 96-97 6.5945013399394785 1103 98-99 6.378898709518731 1103 100-101 5.881460575110058 1104 1 6.30826506528437 1104 2 7.475506517574825 1104 3 7.056203752034335 1104 4 8.956033565716009 1104 5 8.890263332835413 1104 6 8.035644685717873 1104 7 7.797624299404202 1104 8 6.8547892771445795 1104 9 6.828849596452951 1104 10-11 6.251936199913704 1104 12-13 6.01758671002678 1104 14-15 6.621705548467261 1104 16-17 7.378749875048069 1104 18-19 7.930296414560843 1104 20-21 8.264124397578701 1104 22-23 8.061395006658003 1104 24-25 8.10270187390502 1104 26-27 8.284485249002259 1104 28-29 7.95337913105941 1104 30-31 7.59118269387632 1104 32-33 7.418836030618628 1104 34-35 10.300306714424337 1104 36-37 -3.846673187828536 1104 38-39 -2.4247719117894917 1104 40-41 6.369132661701347 1104 42-43 8.658553288516654 1104 44-45 0.06324955288352996 1104 46-47 -0.718841480579762 1104 48-49 7.277758589680502 1104 50-51 -6.427381832763835 1104 52-53 3.6408582052848963 1104 54-55 6.82658378830417 1104 56-57 7.396460463970552 1104 58-59 7.6086245755881095 1104 60-61 7.671915050044333 1104 62-63 7.5554496479850215 1104 64-65 7.644025638024782 1104 66-67 7.396522526664601 1104 68-69 7.386744429384208 1104 70-71 7.42693566007306 1104 72-73 7.346794097506027 1104 74-75 7.337767118855663 1104 76-77 7.2625619634012075 1104 78-79 7.027728168174466 1104 80-81 6.754365721292249 1104 82-83 6.672961982002011 1104 84-85 6.600085900592237 1104 86-87 6.405979765039758 1104 88-89 6.261134776510859 1104 90-91 6.1209357539034315 1104 92-93 5.793704694929309 1104 94-95 5.557702649237497 1104 96-97 5.257153481407885 1104 98-99 5.025785213165543 1104 100-101 4.896697586110729 1105 1 5.889786225853417 1105 2 6.986296511713565 1105 3 6.779743446945897 1105 4 8.600393714150545 1105 5 8.601348343498135 1105 6 7.753896149807428 1105 7 7.40549625512114 1105 8 6.313846533924039 1105 9 6.296232681650327 1105 10-11 5.779149721945817 1105 12-13 5.447006080895736 1105 14-15 5.717371874111151 1105 16-17 6.493385802693293 1105 18-19 7.310632870363349 1105 20-21 7.678134090058979 1105 22-23 7.537707862016575 1105 24-25 7.50141134306331 1105 26-27 7.452568941741113 1105 28-29 7.196785469312591 1105 30-31 7.093409328932722 1105 32-33 6.922334093095671 1105 34-35 9.483101687597966 1105 36-37 -4.289118772527477 1105 38-39 -2.7552335129254217 1105 40-41 5.6711775764708925 1105 42-43 8.015577863739903 1105 44-45 -3.8364241598067217 1105 46-47 -5.114205217106491 1105 48-49 5.057197484670144 1105 50-51 -10.916258571359894 1105 52-53 0.16489592074986703 1105 54-55 5.126700618418127 1105 56-57 6.238359644001381 1105 58-59 6.644987472983718 1105 60-61 6.466517678765797 1105 62-63 6.622185302073063 1105 64-65 6.458672920901524 1105 66-67 6.318229888154374 1105 68-69 6.329414009194547 1105 70-71 6.219921265780645 1105 72-73 6.168415482626241 1105 74-75 6.297497238951138 1105 76-77 6.046864877664305 1105 78-79 5.810282142655485 1105 80-81 5.9059926691589375 1105 82-83 5.65395418779158 1105 84-85 5.561843975775137 1105 86-87 5.589247824083158 1105 88-89 5.3806052378734766 1105 90-91 5.087506925414324 1105 92-93 5.096338735498948 1105 94-95 4.936007199034394 1105 96-97 4.84001890530592 1105 98-99 4.694667723028756 1105 100-101 4.158559833836373 1106 1 6.067742340187127 1106 2 7.295510883044393 1106 3 6.806876048851461 1106 4 8.383293965250747 1106 5 8.366526563398924 1106 6 7.4542130872598555 1106 7 7.229440015674786 1106 8 6.517100118882528 1106 9 6.428068162896555 1106 10-11 5.893187929422833 1106 12-13 5.667297733345055 1106 14-15 6.119086157950619 1106 16-17 6.707444570639961 1106 18-19 7.151426701347546 1106 20-21 7.6677816748599845 1106 22-23 7.384302917004895 1106 24-25 7.458675750703051 1106 26-27 7.496374772165353 1106 28-29 7.413678738602247 1106 30-31 7.000729402950412 1106 32-33 7.061333804374186 1106 34-35 9.842375990510561 1106 36-37 -0.20682964857846997 1106 38-39 0.868673719985324 1106 40-41 7.1496526239349585 1106 42-43 8.537578222454478 1106 44-45 -3.638353125571353 1106 46-47 -4.844826974457952 1106 48-49 5.0931714166193665 1106 50-51 -10.745428190859968 1106 52-53 0.467301058242537 1106 54-55 5.347895619161804 1106 56-57 6.404040173674076 1106 58-59 6.929317989095249 1106 60-61 6.756812918360886 1106 62-63 6.7872748276074155 1106 64-65 6.745250652600522 1106 66-67 6.733399468283334 1106 68-69 6.406817068767413 1106 70-71 6.65213602207346 1106 72-73 6.505085298981875 1106 74-75 6.483428081405446 1106 76-77 6.417179934052488 1106 78-79 6.355972865071646 1106 80-81 6.069814226155184 1106 82-83 5.893177876952276 1106 84-85 5.92726968686117 1106 86-87 5.558685123399853 1106 88-89 5.542534794204785 1106 90-91 5.466335984794711 1106 92-93 5.5504311438736185 1106 94-95 5.2132266361149835 1106 96-97 4.914649144944756 1106 98-99 4.42777839085031 1106 100-101 4.024005121114254 1107 1 5.993799457360311 1107 2 7.302582244936314 1107 3 6.733022195516742 1107 4 8.079976536215032 1107 5 8.44761514342174 1107 6 6.968323829575656 1107 7 7.1650466263306605 1107 8 6.342750120611253 1107 9 6.399843220808943 1107 10-11 5.829630380067645 1107 12-13 5.516558702124225 1107 14-15 5.8244715778586595 1107 16-17 6.8015919619893985 1107 18-19 7.267280433671303 1107 20-21 7.719358274797752 1107 22-23 7.618045094510691 1107 24-25 7.698414021283554 1107 26-27 7.663931733753017 1107 28-29 7.436351006347639 1107 30-31 7.398655361530089 1107 32-33 7.193957494862715 1107 34-35 9.58686048382038 1107 36-37 -0.3076870976674293 1107 38-39 0.6445648792703267 1107 40-41 7.493315539261861 1107 42-43 8.688982813956049 1107 44-45 -3.6131984044151793 1107 46-47 -4.68742196806166 1107 48-49 5.345754568436259 1107 50-51 -10.715736423062715 1107 52-53 0.4253145768955129 1107 54-55 5.569338761297821 1107 56-57 6.704913613497052 1107 58-59 7.209250914448777 1107 60-61 7.227247347707774 1107 62-63 7.287512095525681 1107 64-65 6.949624334445417 1107 66-67 6.955225955853781 1107 68-69 6.826748870447741 1107 70-71 6.933554789144647 1107 72-73 6.729793143949546 1107 74-75 6.700935342063051 1107 76-77 6.631759161656817 1107 78-79 6.466341343445254 1107 80-81 6.361932523012353 1107 82-83 6.102852703215113 1107 84-85 6.200467055737153 1107 86-87 6.14693323044269 1107 88-89 5.979654474044361 1107 90-91 5.880699985762799 1107 92-93 5.842401202304627 1107 94-95 5.473662967062671 1107 96-97 5.184395972728874 1107 98-99 5.0591219582534155 1107 100-101 4.621481178231427 1108 1 5.820973299684191 1108 2 6.533309330260032 1108 3 6.672725957814322 1108 4 8.157803567092106 1108 5 8.178259189047349 1108 6 7.4427604437475345 1108 7 7.447504875340183 1108 8 6.5322698258460115 1108 9 4.846330555970862 1108 10-11 5.7564492918880426 1108 12-13 5.098827500770941 1108 14-15 5.930053985770201 1108 16-17 6.124264110988303 1108 18-19 6.872299541362658 1108 20-21 7.709701699594607 1108 22-23 7.7127047858427105 1108 24-25 7.450717730846648 1108 26-27 7.551877365864897 1108 28-29 7.499897777387506 1108 30-31 7.195265886512196 1108 32-33 7.176710491996566 1108 34-35 9.75578077940746 1108 36-37 -4.072697593200406 1108 38-39 -2.642141663573021 1108 40-41 5.667264445021221 1108 42-43 8.113756552180678 1108 44-45 -3.54313686943064 1108 46-47 -4.822647408000844 1108 48-49 5.5376391929084825 1108 50-51 -10.895207468746534 1108 52-53 0.3774233571878831 1108 54-55 5.494801215369165 1108 56-57 6.593757700899335 1108 58-59 7.089762353381211 1108 60-61 7.116766025647294 1108 62-63 7.0699309648845485 1108 64-65 6.937654204766513 1108 66-67 6.79931250205296 1108 68-69 6.81364785993166 1108 70-71 6.768686745217504 1108 72-73 6.648277267101701 1108 74-75 6.724165911629772 1108 76-77 6.691122700894358 1108 78-79 6.55517027824283 1108 80-81 6.59605396300633 1108 82-83 6.460009441073339 1108 84-85 6.403099756599051 1108 86-87 6.441013612870462 1108 88-89 6.205338823886327 1108 90-91 6.182714939757094 1108 92-93 6.307459039026321 1108 94-95 5.992738127406335 1108 96-97 5.724576057583457 1108 98-99 5.6707290246749 1108 100-101 5.2776904074518125 1109 1 4.907679255035259 1109 2 6.567240722117635 1109 3 6.337471111398539 1109 4 7.811153374652736 1109 5 7.8357286336622245 1109 6 5.323742217243186 1109 7 4.929383009267379 1109 8 3.42140330280672 1109 9 2.9447870369618414 1109 10-11 2.6917301214355334 1109 12-13 3.2206255550895087 1109 14-15 3.7822711073865207 1109 16-17 3.1611630026147317 1109 18-19 4.303612686205945 1109 20-21 4.762968559896095 1109 22-23 6.295458503778249 1109 24-25 6.421885240524787 1109 26-27 4.890344672200548 1109 28-29 4.633990325154553 1109 30-31 4.744713881792599 1109 32-33 4.610261153198131 1109 34-35 7.058010943000369 1109 36-37 -4.957275956395618 1109 38-39 -3.66434615081954 1109 40-41 3.496078780277937 1109 42-43 4.398660079001925 1109 44-45 -4.85920067415041 1109 46-47 -6.747811739786425 1109 48-49 2.1775139596868343 1109 50-51 -11.141405167251754 1109 52-53 -1.3757597204766192 1109 54-55 2.650634471646093 1109 56-57 4.193604401862583 1109 58-59 5.108160686312697 1109 60-61 5.232937486086534 1109 62-63 5.282871118991597 1109 64-65 4.704967554709466 1109 66-67 4.946202678383937 1109 68-69 4.949244123833861 1109 70-71 5.170408466165007 1109 72-73 4.902693211238546 1109 74-75 4.935079122208652 1109 76-77 5.176644306502105 1109 78-79 5.2713414362176785 1109 80-81 5.05799684879894 1109 82-83 4.9119284768283755 1109 84-85 4.768398319201832 1109 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-14.961563764473102 2313 82-83 -14.446249728302494 2313 84-85 -13.937474095818859 2313 86-87 -13.36406430709718 2313 88-89 -12.803859484798554 2313 90-91 -12.200351042340777 2313 92-93 -11.589663340504885 2313 94-95 -11.207741105820501 2313 96-97 -10.37836293560008 2313 98-99 -9.393462889557608 2313 100-101 -8.140862447314626 2314 1 -21.595160885619325 2314 2 -20.338628901901842 2314 3 -20.82833900491198 2314 4 -22.781759798190112 2314 5 -22.748258382837637 2314 6 -23.57401271919176 2314 7 -23.713570737013388 2314 8 -24.62241601014973 2314 9 -25.93322215968409 2314 10-11 -26.289876586706203 2314 12-13 -26.295874309665695 2314 14-15 -26.58938158398486 2314 16-17 -25.67938338634929 2314 18-19 -24.984729212630953 2314 20-21 -24.45856241116152 2314 22-23 -24.42187987869403 2314 24-25 -24.149388765425982 2314 26-27 -23.851743507404535 2314 28-29 -23.86857932591388 2314 30-31 -23.869297478770022 2314 32-33 -23.65103178702367 2314 34-35 -20.622946590134603 2314 36-37 -18.90682964857847 2314 38-39 -17.954175742380265 2314 40-41 -19.512847376065043 2314 42-43 -19.458927153889608 2314 44-45 -17.356901512668127 2314 46-47 -18.308536651877308 2314 48-49 -17.811801701660205 2314 50-51 -15.5529550725804 2314 52-53 -18.71549464068219 2314 54-55 -20.574953843203787 2314 56-57 -20.85765337471302 2314 58-59 -20.469875559291847 2314 60-61 -20.188079554757394 2314 62-63 -19.72226818314527 2314 64-65 -19.236200960302707 2314 66-67 -18.82022956397473 2314 68-69 -18.36939260865194 2314 70-71 -17.78496075212009 2314 72-73 -17.31910824940522 2314 74-75 -16.788480520745093 2314 76-77 -16.223680281001275 2314 78-79 -15.736097027401474 2314 80-81 -15.24806211793084 2314 82-83 -14.772951155305787 2314 84-85 -14.27501525937539 2314 86-87 -13.704486919610902 2314 88-89 -13.138244775687687 2314 90-91 -12.54145971413002 2314 92-93 -11.896880684083369 2314 94-95 -11.207741105820501 2314 96-97 -10.44408741419503 2314 98-99 -9.557705480117432 2314 100-101 -8.327607782111553 2315 1 -21.595160885619325 2315 2 -20.338628901901842 2315 3 -20.82833900491198 2315 4 -16.146164268072916 2315 5 -22.748258382837637 2315 6 -23.57401271919176 2315 7 -23.713570737013388 2315 8 -24.62241601014973 2315 9 -19.78495287650066 2315 10-11 -17.859514090113127 2315 12-13 -13.289878125419307 2315 14-15 -13.118136012984587 2315 16-17 -20.174341140505735 2315 18-19 -24.984729212630953 2315 20-21 -24.45856241116152 2315 22-23 -24.42187987869403 2315 24-25 -24.149388765425982 2315 26-27 -23.851743507404535 2315 28-29 -23.86857932591388 2315 30-31 -23.869297478770022 2315 32-33 -23.65103178702367 2315 34-35 -20.622946590134603 2315 36-37 -18.90682964857847 2315 38-39 -17.954175742380265 2315 40-41 -19.512847376065043 2315 42-43 -19.458927153889608 2315 44-45 -17.356901512668127 2315 46-47 -18.308536651877308 2315 48-49 -17.811801701660205 2315 50-51 -15.5529550725804 2315 52-53 -18.71549464068219 2315 54-55 -20.574953843203787 2315 56-57 -20.85765337471302 2315 58-59 -20.469875559291847 2315 60-61 -20.188079554757394 2315 62-63 -19.72226818314527 2315 64-65 -19.236200960302707 2315 66-67 -18.82022956397473 2315 68-69 -18.36939260865194 2315 70-71 -17.78496075212009 2315 72-73 -17.31910824940522 2315 74-75 -16.788480520745093 2315 76-77 -16.223680281001275 2315 78-79 -15.736097027401474 2315 80-81 -15.24806211793084 2315 82-83 -14.772951155305787 2315 84-85 -14.27501525937539 2315 86-87 -13.704486919610902 2315 88-89 -13.138244775687687 2315 90-91 -12.54145971413002 2315 92-93 -11.896880684083369 2315 94-95 -11.207741105820501 2315 96-97 -10.44408741419503 2315 98-99 -9.557705480117432 2315 100-101 -8.327607782111553 2316 1 -21.595160885619325 2316 2 -20.338628901901842 2316 3 -20.82833900491198 2316 4 -22.781759798190112 2316 5 -22.748258382837637 2316 6 -23.57401271919176 2316 7 -23.713570737013388 2316 8 -24.62241601014973 2316 9 -25.93322215968409 2316 10-11 -26.289876586706203 2316 12-13 -26.295874309665695 2316 14-15 -26.58938158398486 2316 16-17 -25.67938338634929 2316 18-19 -24.984729212630953 2316 20-21 -24.45856241116152 2316 22-23 -24.42187987869403 2316 24-25 -24.149388765425982 2316 26-27 -23.851743507404535 2316 28-29 -23.86857932591388 2316 30-31 -23.869297478770022 2316 32-33 -23.65103178702367 2316 34-35 -20.622946590134603 2316 36-37 -18.90682964857847 2316 38-39 -17.954175742380265 2316 40-41 -19.512847376065043 2316 42-43 -19.458927153889608 2316 44-45 -17.356901512668127 2316 46-47 -18.308536651877308 2316 48-49 -17.811801701660205 2316 50-51 -15.5529550725804 2316 52-53 -18.71549464068219 2316 54-55 -20.574953843203787 2316 56-57 -20.85765337471302 2316 58-59 -20.469875559291847 2316 60-61 -20.188079554757394 2316 62-63 -19.72226818314527 2316 64-65 -19.236200960302707 2316 66-67 -18.82022956397473 2316 68-69 -18.36939260865194 2316 70-71 -17.78496075212009 2316 72-73 -17.31910824940522 2316 74-75 -16.788480520745093 2316 76-77 -16.223680281001275 2316 78-79 -15.736097027401474 2316 80-81 -15.24806211793084 2316 82-83 -14.772951155305787 2316 84-85 -14.27501525937539 2316 86-87 -13.704486919610902 2316 88-89 -13.138244775687687 2316 90-91 -12.54145971413002 2316 92-93 -11.896880684083369 2316 94-95 -11.207741105820501 2316 96-97 -10.44408741419503 2316 98-99 -9.557705480117432 2316 100-101 -8.327607782111553 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 514528.0 3 28871.0 4 69463.0 5 33518.0 6 26837.0 7 32520.0 8 43280.0 9 54188.0 10 58916.0 11 57296.0 12 52301.0 13 48467.0 14 46676.0 15 50377.0 16 56199.0 17 60838.0 18 63669.0 19 67180.0 20 71824.0 21 77624.0 22 83548.0 23 89622.0 24 96668.0 25 104018.0 26 113115.0 27 122423.0 28 133009.0 29 143076.0 30 154308.0 31 158765.0 32 155129.0 33 154704.0 34 151158.0 35 134527.0 36 105288.0 37 53973.0 38 9211.0 39 80.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4829690969363 19.63318675182706 13.764349482832358 24.119494668404275 2 15.482002364340392 20.585896866901393 40.51711805127421 23.41498271748401 3 18.583981693046045 25.625048390451138 30.097419743026304 25.693550173476513 4 12.865524603045172 16.851640450104522 38.33101065958993 31.951824287260383 5 13.5966815169891 37.673696065866636 35.021350806041475 13.70827161110279 6 30.94684318759193 36.872116026366385 18.209076583393866 13.971964202647813 7 26.9653786555255 32.35929308526792 22.942871771771163 17.732456487435417 8 25.333087371184682 35.5005803987138 21.081409219197738 18.08492301090378 9 26.42117929919029 16.03067291957776 20.88393604756183 36.66421173367012 10-11 25.048288771468428 26.07993630004941 28.759278551293782 20.11249637718838 12-13 25.183953304912286 24.043011175933923 28.598937140459647 22.174098378694143 14-15 22.593897351588872 25.402485997279744 26.117274665147615 25.88634198598377 16-17 22.441799809507007 28.442524027281145 26.693760664057987 22.42191549915386 18-19 22.431454390346214 27.708511903279 28.21511750435469 21.64491620202009 20-21 23.254917971902042 26.855054619128005 28.01212295746153 21.877904451508424 22-23 23.068244048537892 26.683969945060632 27.742589161935673 22.505196844465804 24-25 22.83229552104572 26.822039173587115 27.73589905294101 22.60976625242615 26-27 22.73559342983265 27.060877588810317 27.60366560371782 22.59986337763921 28-29 22.781551675495447 27.124778054762277 27.640571928272124 22.453098341470152 30-31 23.082140953171947 26.865949908850222 27.527198886106678 22.52471025187115 32-33 22.868708223856398 27.021441649019945 27.390977050157044 22.718873076966613 34-35 23.204924639345183 26.937174674380937 27.430165034391944 22.427735651881935 36-37 23.64280268394678 27.00406765130465 27.04283148749937 22.310298177249198 38-39 23.450138845908477 26.975544530172996 27.106551309526523 22.467765314392008 40-41 23.4056211385101 26.83346675513838 27.278922293712267 22.481989812639256 42-43 23.48147901235434 26.932326553240166 26.902379289897226 22.683815144508266 44-45 23.64823628324563 27.08418097332843 26.740239315982674 22.527343427443267 46-47 23.164175697334237 26.94061968734738 27.021803740767208 22.873400874551177 48-49 23.550889753865064 27.029948116687198 26.686811875981725 22.732350253466013 50-51 23.758474351270653 27.121500569412177 26.47813141569497 22.6418936636222 52-53 22.92159135276849 27.107120056803698 27.038562753676203 22.932725836751608 54-55 22.657170467142862 27.010876675953988 27.168343713717537 23.163609143185614 56-57 22.77704419353256 27.132023996217995 26.999896785872696 23.09103502437675 58-59 22.842138125611307 27.02800274331566 27.13054495998778 22.999314171085253 60-61 22.87293018161785 27.14295375185766 27.09447724045108 22.8896388260734 62-63 22.836537946415696 27.060116063341145 26.993125377584988 23.110220612658175 64-65 22.657653507761758 26.894876298549793 27.042614396887423 23.404855796801034 66-67 22.688800537401796 26.97167144469966 27.007862874765532 23.331665143133012 68-69 22.693566073582 27.007174213525726 27.001408066574474 23.297851646317802 70-71 22.560383755919755 26.831624970548983 27.015513450452588 23.592477823078674 72-73 22.680206211808503 26.944392179392224 26.912962981554088 23.46243862724518 74-75 22.65184371495838 27.08419194336261 26.808680427872627 23.455283913806387 76-77 22.551868780919058 26.907146985166637 26.902379604171166 23.63860462974314 78-79 22.501820183761193 26.901289846550775 26.86271986761115 23.73417010207688 80-81 22.574050733912387 27.009197109293837 26.8078312213201 23.60892093547368 82-83 22.73391984960891 26.873710659551342 26.86631154100737 23.526057949832374 84-85 22.66804354547253 26.86097876815755 26.74659092873068 23.724386757639245 86-87 22.427534223443526 27.01284923164472 26.765323448109463 23.79429309680229 88-89 22.509466408596225 26.742296999483433 26.743859878756382 24.004376713163953 90-91 22.519929442317572 26.696587083992583 26.84271152502306 23.940771948666782 92-93 22.571281189770584 26.519562255196565 26.873329119432988 24.03582743559986 94-95 22.37887930531402 26.520322625406717 26.938598982507056 24.1621990867722 96-97 22.353583071266613 26.40002012775642 26.78488282920131 24.461513971775663 98-99 22.18137618023733 26.426639449332086 26.788665856759863 24.603318513670718 100-101 22.43808308546794 26.553105307919424 26.54786644477529 24.460945161837348 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 41706.0 1 44980.0 2 33144.0 3 19275.5 4 25444.5 5 32508.5 6 34387.5 7 32779.0 8 29947.0 9 26887.0 10 23786.5 11 20789.5 12 17716.0 13 14734.5 14 12277.0 15 10385.5 16 8891.0 17 7962.0 18 7687.5 19 7708.0 20 8010.0 21 8671.0 22 9593.0 23 10866.5 24 12620.5 25 14810.0 26 17586.5 27 21537.0 28 26785.5 29 33559.5 30 42024.5 31 51644.0 32 61903.0 33 70616.0 34 77865.0 35 84321.5 36 89737.5 37 94380.0 38 96738.0 39 98060.0 40 99036.0 41 100455.5 42 102774.5 43 104897.5 44 106829.0 45 108875.5 46 110839.5 47 111732.0 48 111593.5 49 110707.5 50 107917.0 51 104003.5 52 100379.5 53 96063.5 54 90366.5 55 83000.0 56 74964.5 57 66443.0 58 58189.5 59 50461.0 60 42446.0 61 35206.5 62 28981.0 63 23243.5 64 18358.0 65 14395.0 66 11108.5 67 8666.5 68 6897.0 69 5520.5 70 4455.5 71 3560.0 72 2803.5 73 2239.5 74 1742.5 75 1312.0 76 1018.0 77 717.0 78 479.0 79 354.5 80 255.0 81 156.5 82 95.0 83 64.0 84 39.0 85 22.5 86 14.5 87 11.0 88 7.5 89 7.0 90 4.5 91 2.5 92 2.5 93 3.0 94 2.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.399340387680413 2 21.39900736053266 3 17.16835471359953 4 22.40755045591359 5 22.246357263931777 6 16.10479599355112 7 14.961258992164373 8 12.07332694120604 9 3.0412740847936583 10-11 6.526986990084533 12-13 3.557279231472273 14-15 7.4094514139849545 16-17 11.485999918324948 18-19 14.576005825386792 20-21 15.016116443316077 22-23 12.903320896101858 24-25 15.453725043814066 26-27 12.979862498324799 28-29 12.997678587964895 30-31 13.849529246858241 32-33 12.34263892092302 34-35 19.14704787826046 36-37 23.918337602101005 38-39 22.958885814251374 40-41 18.127633948522863 42-43 18.037072421038346 44-45 23.379023430961862 46-47 12.820984391437465 48-49 21.464275504904244 50-51 21.19776751023958 52-53 11.27873509502202 54-55 12.847097400950306 56-57 12.370046077383085 58-59 13.408483967244852 60-61 12.821516429626879 62-63 12.767090360790915 64-65 13.84311602976423 66-67 12.193308742624081 68-69 13.217007736698038 70-71 11.810140590372582 72-73 11.195837793347165 74-75 11.274133683654119 76-77 11.32349819998539 78-79 11.717752877751428 80-81 11.69517720322766 82-83 10.215147616152564 84-85 10.144271501676352 86-87 10.24696925164371 88-89 11.674341437377379 90-91 9.171964520817648 92-93 11.187238905853397 94-95 11.295026967146498 96-97 10.84200363856604 98-99 10.70719666489704 100-101 10.79537121023446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3477194.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.6668985276156 #Duplication Level Percentage of deduplicated Percentage of total 1 98.67937777974352 95.39029398602712 2 0.9104096234387583 1.760129493750383 3 0.18485180007043422 0.5360715060016724 4 0.07689265462320519 0.2973189776792147 5 0.03979147723707367 0.1923259346170063 6 0.023895484066219875 0.13859414000985193 7 0.016460628376884768 0.11138385251063768 8 0.011062775374275786 0.08555233476311377 9 0.009333850939616495 0.08120469794866175 >10 0.04393372383684406 0.7468260254547952 >50 0.002604482793957787 0.17369610773217445 >100 0.001266769598296382 0.21319693195559422 >500 2.973747527957963E-5 0.01926001768563463 >1k 5.947495039509887E-5 0.07350427624823662 >5k 2.973747519754939E-5 0.1806417176159243 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6284 0.18072043147434397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.8758821049386372E-5 0.0 7 0.0 0.0 0.0 2.8758821049386372E-5 0.0 8 0.0 0.0 0.0 2.8758821049386372E-5 0.0 9 0.0 0.0 0.0 2.8758821049386372E-5 0.0 10-11 0.0 1.4379410524693186E-5 0.0 2.8758821049386372E-5 0.0 12-13 0.0 4.313823157407956E-5 0.0 2.8758821049386372E-5 2.156911578703978E-4 14-15 0.0 5.7517642098772744E-5 0.0 5.7517642098772744E-5 2.4444997891978415E-4 16-17 0.0 8.627646314815912E-5 0.0 7.189705262346593E-5 2.5882938944447734E-4 18-19 0.0 1.1503528419754549E-4 0.0 8.627646314815912E-5 2.5882938944447734E-4 20-21 0.0 1.1503528419754549E-4 0.0 1.006558736728523E-4 2.8758821049386374E-4 22-23 0.0 1.1503528419754549E-4 0.0 1.4379410524693187E-4 2.8758821049386374E-4 24-25 0.0 1.1503528419754549E-4 0.0 2.7320879996917054E-4 3.163470315432501E-4 26-27 0.0 1.1503528419754549E-4 0.0 3.8824408416671607E-4 3.163470315432501E-4 28-29 0.0 1.1503528419754549E-4 0.0 8.052469893828184E-4 3.163470315432501E-4 30-31 0.0 1.1503528419754549E-4 0.0 0.0028902615154633307 3.163470315432501E-4 32-33 0.0 1.1503528419754549E-4 0.0 0.009849896209414833 3.451058525926365E-4 34-35 0.0 1.1503528419754549E-4 0.0 0.01967103359778028 3.451058525926365E-4 36-37 0.0 1.1503528419754549E-4 0.0 0.03574721456438726 3.451058525926365E-4 38-39 0.0 1.1503528419754549E-4 0.0 0.06000528011954467 3.7386467364202287E-4 40-41 0.0 1.1503528419754549E-4 0.0 0.08831833944266555 3.7386467364202287E-4 42-43 0.0 1.1503528419754549E-4 0.0 0.11773861337618782 3.7386467364202287E-4 44-45 0.0 1.2941469472223867E-4 0.0 0.1557002571613778 3.7386467364202287E-4 46-47 0.0 1.4379410524693187E-4 0.0 0.1990110416617537 3.7386467364202287E-4 48-49 2.8758821049386372E-5 1.4379410524693187E-4 0.0 0.24465129066712987 4.170029052161024E-4 50-51 2.8758821049386372E-5 1.4379410524693187E-4 0.0 0.29841590661895767 4.6014113679018195E-4 52-53 2.8758821049386372E-5 1.4379410524693187E-4 0.0 0.36996785338983096 5.464175999383411E-4 54-55 2.8758821049386372E-5 1.7255292629631824E-4 0.0 0.454561925506601 6.039352420371139E-4 56-57 2.8758821049386372E-5 2.013117473457046E-4 0.0 0.5494085173274772 6.039352420371139E-4 58-59 2.8758821049386372E-5 2.013117473457046E-4 0.0 0.6545651464945585 6.039352420371139E-4 60-61 2.8758821049386372E-5 2.013117473457046E-4 0.0 0.7656748516188627 6.18314652561807E-4 62-63 2.8758821049386372E-5 2.013117473457046E-4 0.0 0.8817023151426121 7.189705262346593E-4 64-65 2.8758821049386372E-5 2.013117473457046E-4 0.0 1.0077522278020727 7.76488168333432E-4 66-67 2.8758821049386372E-5 2.013117473457046E-4 0.0 1.1469017834495285 7.76488168333432E-4 68-69 2.8758821049386372E-5 2.013117473457046E-4 0.0 1.2993810526533751 7.76488168333432E-4 70-71 2.8758821049386372E-5 2.013117473457046E-4 0.0 1.4623285327191984 7.76488168333432E-4 72-73 2.8758821049386372E-5 2.013117473457046E-4 0.0 1.6371821646994675 8.052469893828184E-4 74-75 2.8758821049386372E-5 2.013117473457046E-4 0.0 1.8164790345318669 8.627646314815912E-4 76-77 2.8758821049386372E-5 2.013117473457046E-4 0.0 2.0181071289091146 8.915234525309776E-4 78-79 2.8758821049386372E-5 2.013117473457046E-4 0.0 2.241606306694421 9.490410946297503E-4 80-81 2.8758821049386372E-5 2.013117473457046E-4 0.0 2.48276340060405 9.490410946297503E-4 82-83 2.8758821049386372E-5 2.013117473457046E-4 0.0 2.73372725249152 9.490410946297503E-4 84-85 5.7517642098772744E-5 2.013117473457046E-4 0.0 2.997043018019702 9.490410946297503E-4 86-87 5.7517642098772744E-5 2.013117473457046E-4 0.0 3.2790520172299846 9.777999156791368E-4 88-89 5.7517642098772744E-5 2.013117473457046E-4 0.0 3.5821987499115666 0.0010496969683026027 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2525 0.0 50.08721 1 GTATCAA 4630 0.0 46.468796 1 ATCAACG 5795 0.0 36.675903 3 TCAACGC 6000 0.0 35.9126 4 CAACGCA 6030 0.0 34.70594 5 TATCAAC 6260 0.0 34.118404 2 AACGCAG 6575 0.0 32.22195 6 GTACATG 8340 0.0 28.093218 1 ACGCAGA 7770 0.0 27.05016 7 TACATGG 8600 0.0 26.88509 2 CGCAGAG 8145 0.0 26.091368 8 ACATGGG 8815 0.0 25.710587 3 GCAGAGT 9065 0.0 22.388058 9 CATGGGG 6905 0.0 21.727356 4 AGAGTAC 8350 0.0 19.181341 10-11 GTGGTAT 1330 0.0 18.942308 1 CAGAGTA 8845 0.0 18.082869 10-11 ATGGGGG 4420 0.0 17.933077 5 GAGTACA 6380 0.0 17.9275 1 GTATTAC 580 0.0 17.37467 1 >>END_MODULE