FastQCFastQC Report
Sat 18 Jun 2016
SRR1930931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477194
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.11431631367131083No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.054.731681
GTATCAA67350.039.1277241
CAACGCA80900.032.0261465
ATCAACG82050.031.532593
TCAACGC82400.031.5116124
TATCAAC87600.029.9145852
AACGCAG87800.029.779526
GTACATG107350.025.3886181
ACGCAGA102950.025.1667337
CGCAGAG107600.023.8586358
TACATGG112300.023.7154352
ACATGGG111200.023.4373913
CATGGGG80600.020.3186344
GCAGAGT125250.019.8903379
GTGGTAT18400.019.0960311
AGAGTAC111750.018.938610-11
CAGAGTA120250.018.13263710-11
GAGTACT64700.017.67556612-13
GAGTACA83900.017.204451
ATGGGGG47200.016.9904675
Produced by FastQC (version 0.11.5)