##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930931_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3477194 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.268831707405454 31.0 31.0 33.0 30.0 34.0 2 31.392022130487973 31.0 31.0 34.0 30.0 34.0 3 31.475236929547215 31.0 31.0 34.0 30.0 34.0 4 35.34339930415157 37.0 35.0 37.0 33.0 37.0 5 35.12880356977494 37.0 35.0 37.0 32.0 37.0 6 35.165982973627585 37.0 35.0 37.0 32.0 37.0 7 35.043168715924395 37.0 35.0 37.0 32.0 37.0 8 35.0183205193613 37.0 35.0 37.0 32.0 37.0 9 36.53029799315195 38.0 35.0 39.0 32.0 39.0 10-11 36.453830013510895 38.0 35.0 39.0 32.0 39.0 12-13 36.35674282194206 38.0 35.0 39.0 32.0 39.0 14-15 37.38417169131202 39.0 36.0 41.0 32.0 41.0 16-17 37.34615698750199 39.0 36.0 41.0 32.0 41.0 18-19 37.32323505677279 39.0 36.0 40.0 32.0 41.0 20-21 37.280298280740155 39.0 36.0 40.0 31.0 41.0 22-23 37.151297856835136 39.0 36.0 40.0 31.0 41.0 24-25 37.031346683561516 39.0 36.0 40.0 31.0 41.0 26-27 36.76235363916997 39.0 35.5 40.0 30.5 41.0 28-29 36.577863645226586 38.0 35.0 40.0 30.0 41.0 30-31 36.34562178584226 38.0 35.0 40.0 30.0 41.0 32-33 36.117792104783334 38.0 35.0 40.0 30.0 41.0 34-35 36.33678477531021 38.0 35.0 40.0 30.0 41.0 36-37 36.401804012085606 38.0 35.0 40.0 30.0 41.0 38-39 36.373588876548155 38.0 35.0 40.0 30.0 41.0 40-41 36.27811332931093 38.0 35.0 40.0 30.0 41.0 42-43 36.14362572234968 38.0 35.0 40.0 30.0 41.0 44-45 35.9757133194179 38.0 35.0 40.0 29.0 41.0 46-47 35.76792149071924 38.0 34.0 40.0 28.5 41.0 48-49 35.57998547104361 38.0 34.0 40.0 28.0 41.0 50-51 35.31541826541746 38.0 34.0 40.0 27.0 41.0 52-53 35.099334549639735 38.0 34.0 40.0 27.0 41.0 54-55 34.92607228702224 38.0 33.0 40.0 26.0 41.0 56-57 34.66856091434646 37.5 33.0 40.0 26.0 41.0 58-59 34.42359169491262 37.0 33.0 40.0 26.0 41.0 60-61 34.110253123639346 37.0 32.5 40.0 26.0 41.0 62-63 33.79949982658431 36.0 32.0 39.0 25.0 41.0 64-65 33.48396566311802 36.0 32.0 39.0 24.5 40.5 66-67 33.14694866032784 35.5 31.5 39.0 23.5 40.0 68-69 32.77921910598028 35.0 31.0 38.5 23.0 40.0 70-71 32.38028565561772 35.0 31.0 38.0 22.0 40.0 72-73 31.96336226854182 35.0 31.0 37.0 22.0 39.5 74-75 31.444005425064002 34.0 30.0 36.5 20.0 39.0 76-77 30.06181737343387 33.0 28.5 35.0 19.5 38.0 78-79 30.83897777920933 34.0 30.0 36.0 20.0 38.0 80-81 30.850618487205487 34.0 30.0 35.5 20.0 37.0 82-83 30.585888650446307 34.0 30.0 35.0 20.0 37.0 84-85 30.27295169610899 34.0 30.0 35.0 18.5 36.5 86-87 29.943227498954617 34.0 29.5 35.0 18.0 36.0 88-89 29.61089200084896 34.0 29.0 35.0 16.0 36.0 90-91 29.28210792955469 34.0 29.0 35.0 9.0 35.0 92-93 28.9307686024996 34.0 29.0 35.0 4.5 35.0 94-95 28.520509784613687 33.0 29.0 35.0 2.0 35.0 96-97 28.066236166288107 33.0 28.5 35.0 2.0 35.0 98-99 27.58398783616905 33.0 27.0 35.0 2.0 35.0 100-101 26.372913763224027 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.430860519999019 1101 2 0.5103377704965162 1101 3 0.5168558540228965 1101 4 0.3181325275814828 1101 5 0.42595860160136567 1101 6 0.46670897569844527 1101 7 0.41168592072562404 1101 8 0.4165591374132802 1101 9 0.4915514498739242 1101 10-11 0.4822629845770905 1101 12-13 0.5876367228184947 1101 14-15 0.7671770500120232 1101 16-17 0.6137695093598268 1101 18-19 0.6697704291463324 1101 20-21 0.7567198906614934 1101 22-23 0.7502711374630238 1101 24-25 0.6900587912489868 1101 26-27 0.6734142846054922 1101 28-29 0.7120296115906584 1101 30-31 0.7339510582380768 1101 32-33 0.6957648567265551 1101 34-35 0.5717532960339398 1101 36-37 0.7304438738877366 1101 38-39 0.8750455557887236 1101 40-41 0.7724791114117195 1101 42-43 0.7473086115588572 1101 44-45 0.7326868818818255 1101 46-47 0.7458498680387677 1101 48-49 0.7797239697289697 1101 50-51 0.9004778602734334 1101 52-53 0.69397145672243 1101 54-55 0.6793823331471884 1101 56-57 0.8799601738567091 1101 58-59 0.6778380452167454 1101 60-61 0.6229984212972468 1101 62-63 0.7139881301531759 1101 64-65 0.701169905819647 1101 66-67 0.7191945822495143 1101 68-69 0.6860130816071219 1101 70-71 0.7217351443242492 1101 72-73 0.7196157800277341 1101 74-75 0.7439648081654546 1101 76-77 0.7993376553493157 1101 78-79 0.8040744281305336 1101 80-81 0.6994122590950305 1101 82-83 0.8483432039734495 1101 84-85 0.7919156671138587 1101 86-87 0.6888630759358669 1101 88-89 0.741858002306838 1101 90-91 0.6661655510110798 1101 92-93 0.8215139173756043 1101 94-95 0.8324995857068416 1101 96-97 0.7560241678171309 1101 98-99 0.814145253066048 1101 100-101 0.912607093206617 1102 1 0.4323937400564901 1102 2 0.49329253312103916 1102 3 0.46080341355353127 1102 4 0.21510882651855923 1102 5 0.3645351131912946 1102 6 0.3609506702497356 1102 7 0.47596476003026567 1102 8 0.436879298084321 1102 9 0.6088666729683041 1102 10-11 0.5536428478816191 1102 12-13 0.6053842879086844 1102 14-15 0.7166815549860814 1102 16-17 0.6745705886077573 1102 18-19 0.6874059345269359 1102 20-21 0.7249687709330814 1102 22-23 0.6682777429051328 1102 24-25 0.6147928883257023 1102 26-27 0.6608983443289702 1102 28-29 0.7262129829721644 1102 30-31 0.7891930203427862 1102 32-33 0.7491708638219308 1102 34-35 0.7362665051807866 1102 36-37 0.694619948357925 1102 38-39 0.818805046116843 1102 40-41 0.876649904307996 1102 42-43 0.738749073974553 1102 44-45 0.8057984586053735 1102 46-47 0.8339870013255464 1102 48-49 0.7297314403762769 1102 50-51 0.7221517717306298 1102 52-53 0.6994836651500194 1102 54-55 0.718439919676392 1102 56-57 0.6777710135922135 1102 58-59 0.8093574880106971 1102 60-61 0.741510250980383 1102 62-63 0.5234093113493756 1102 64-65 0.5772579274185077 1102 66-67 0.6529434051882248 1102 68-69 0.6831242865356586 1102 70-71 0.5372952865097744 1102 72-73 0.5586103003948004 1102 74-75 0.6786457246575104 1102 76-77 0.7185043828235784 1102 78-79 0.7613570931015374 1102 80-81 0.7334506130508274 1102 82-83 0.9368781926632366 1102 84-85 0.9871332366864323 1102 86-87 0.8914487885834994 1102 88-89 0.8489076004555081 1102 90-91 0.8331084578920667 1102 92-93 0.8888578937753095 1102 94-95 0.9639560492066472 1102 96-97 0.9518754393907969 1102 98-99 0.9519652313143006 1102 100-101 1.029080131119283 1103 1 0.3240856809031527 1103 2 0.42145353800016494 1103 3 0.45223679675464723 1103 4 0.3809962317481208 1103 5 0.4142943124359775 1103 6 0.4401931130533683 1103 7 0.4595142695812413 1103 8 0.46308061048556226 1103 9 0.5273176905556696 1103 10-11 0.5251575786760299 1103 12-13 0.5184882851269776 1103 14-15 0.6727737769731945 1103 16-17 0.5582951303624384 1103 18-19 0.6267457370604106 1103 20-21 0.5744989248549999 1103 22-23 0.6345591407374229 1103 24-25 0.7263528713483893 1103 26-27 0.665445685930699 1103 28-29 0.7306027089715954 1103 30-31 0.8502103664115808 1103 32-33 0.7551495840141769 1103 34-35 0.7412777223382747 1103 36-37 0.7726099735640517 1103 38-39 0.7074203118453042 1103 40-41 0.9483048341033253 1103 42-43 0.8628502474141939 1103 44-45 0.8707350040178525 1103 46-47 0.7445607963627694 1103 48-49 0.7229307679655363 1103 50-51 0.7830562037761908 1103 52-53 0.7249906336466907 1103 54-55 0.6967621286582855 1103 56-57 0.8723260519087361 1103 58-59 0.7990580276469217 1103 60-61 0.7437314090266014 1103 62-63 0.6872211093489753 1103 64-65 0.6340362026366932 1103 66-67 0.5193113601265509 1103 68-69 0.5642661906930044 1103 70-71 0.4632587988845387 1103 72-73 0.44978855977408827 1103 74-75 0.5913314759595814 1103 76-77 0.75041929947756 1103 78-79 0.7719669896908528 1103 80-81 0.6437956411834342 1103 82-83 0.6599700075411619 1103 84-85 0.7191021045887815 1103 86-87 0.693076792251194 1103 88-89 0.721131771604707 1103 90-91 0.7782693650154897 1103 92-93 0.8229057408606408 1103 94-95 0.8438585286129872 1103 96-97 0.795891986090183 1103 98-99 0.9769141261180287 1103 100-101 1.1200778518027832 1104 1 0.3983548679859652 1104 2 0.47082086623790076 1104 3 0.4572948694910792 1104 4 0.2627047994360012 1104 5 0.3623528887044216 1104 6 0.33643748698284526 1104 7 0.3507894993456091 1104 8 0.3855083515600626 1104 9 0.5138229285674853 1104 10-11 0.4268461136417585 1104 12-13 0.4887784837410578 1104 14-15 0.5172382262153548 1104 16-17 0.5430197521855078 1104 18-19 0.6736660796644358 1104 20-21 0.5755076246865727 1104 22-23 0.599841567767669 1104 24-25 0.5945725510925115 1104 26-27 0.5949154024805452 1104 28-29 0.5398401048337078 1104 30-31 0.5259264942177069 1104 32-33 0.6165218381214643 1104 34-35 0.5999569873183574 1104 36-37 0.6253803216257126 1104 38-39 0.6223677919397304 1104 40-41 0.6468265304440735 1104 42-43 0.6806545281203569 1104 44-45 0.6897883742471222 1104 46-47 0.6721218946038547 1104 48-49 0.5667681443763897 1104 50-51 0.6104162163748938 1104 52-53 0.7204179503028953 1104 54-55 0.6624730635117118 1104 56-57 0.7000491027595146 1104 58-59 0.7341623283694432 1104 60-61 0.7613222016775936 1104 62-63 0.6343763839880623 1104 64-65 0.6851968617669328 1104 66-67 0.6912289144183319 1104 68-69 0.6983844549857423 1104 70-71 0.7065446545721983 1104 72-73 0.6524376274070924 1104 74-75 0.8185099954565054 1104 76-77 0.8904471870611381 1104 78-79 0.8939249410501944 1104 80-81 0.7023466289300266 1104 82-83 0.8184111283504762 1104 84-85 0.8434602722914128 1104 86-87 0.7639147938388184 1104 88-89 0.8316467126456573 1104 90-91 0.8020660856034034 1104 92-93 0.9050572263692978 1104 94-95 0.9590579085518307 1104 96-97 0.975391593347112 1104 98-99 1.1141629586702884 1104 100-101 1.3962970580543121 1105 1 0.1857296943010418 1105 2 0.24053486878214514 1105 3 0.22035216021917492 1105 4 0.10944729300240397 1105 5 0.21737848224024958 1105 6 0.21628048042087755 1105 7 0.21103081703827797 1105 8 0.2316445986809228 1105 9 0.34762894536225275 1105 10-11 0.32059692440309107 1105 12-13 0.2859455510235165 1105 14-15 0.4456804764856841 1105 16-17 0.3645064036791865 1105 18-19 0.3820838181456949 1105 20-21 0.3943762075631909 1105 22-23 0.46929430487233503 1105 24-25 0.40435746091170444 1105 26-27 0.3932083351146858 1105 28-29 0.4420426246561604 1105 30-31 0.4497026003668765 1105 32-33 0.39334917619253673 1105 34-35 0.42451756290567033 1105 36-37 0.48530377331758245 1105 38-39 0.3235842137994567 1105 40-41 0.3174028135651099 1105 42-43 0.20586497121656322 1105 44-45 0.25139719983742026 1105 46-47 0.23919699841240316 1105 48-49 0.28453862075981107 1105 50-51 0.2845235228737337 1105 52-53 0.1686736894322607 1105 54-55 0.20725584312751977 1105 56-57 0.3577891453665103 1105 58-59 0.25274569153587123 1105 60-61 0.2258266771533286 1105 62-63 0.1960536189950446 1105 64-65 0.17818036031815865 1105 66-67 0.2258155814303393 1105 68-69 0.21532690671631372 1105 70-71 0.09608360363820623 1105 72-73 0.07146988804418797 1105 74-75 0.268539440771729 1105 76-77 0.23499534798554933 1105 78-79 0.21384896782015161 1105 80-81 0.22388154747416777 1105 82-83 0.30281926873449905 1105 84-85 0.29381980218775183 1105 86-87 0.32976536908885024 1105 88-89 0.35744394025710235 1105 90-91 0.32862642574526646 1105 92-93 0.43399429987591276 1105 94-95 0.19688679413902221 1105 96-97 0.092374659053295 1105 98-99 0.3039993826093692 1105 100-101 0.6071783769351882 1106 1 0.11516047551139863 1106 2 0.15576492731386438 1106 3 0.1290811732291388 1106 4 0.054973422297138086 1106 5 0.1684922472559549 1106 6 0.14490852840113888 1106 7 0.16207677624980477 1106 8 0.1736782916037427 1106 9 0.1807159905165463 1106 10-11 0.2203357626928124 1106 12-13 0.21113715710241365 1106 14-15 0.20971584049348735 1106 16-17 0.1463103880357295 1106 18-19 0.17282373257913974 1106 20-21 0.15458437138201475 1106 22-23 0.24875636424396674 1106 24-25 0.2834394796873525 1106 26-27 0.3415756369686278 1106 28-29 0.30576794115615513 1106 30-31 0.38936046540270297 1106 32-33 0.3262784088058197 1106 34-35 0.3388546627420581 1106 36-37 0.5455350129826684 1106 38-39 0.44707203500211534 1106 40-41 0.4022984144995263 1106 42-43 0.640905042259547 1106 44-45 0.5383841723738882 1106 46-47 0.4187888730897811 1106 48-49 0.4787109306192079 1106 50-51 0.4129278117002215 1106 52-53 0.4121606138484708 1106 54-55 0.5511224800471268 1106 56-57 0.6632797750418362 1106 58-59 0.6920558877789915 1106 60-61 0.5254866638546432 1106 62-63 0.44683940077409545 1106 64-65 0.4561082586881966 1106 66-67 0.4318435174216617 1106 68-69 0.6097208978096944 1106 70-71 0.4483996093076499 1106 72-73 0.49940489508724184 1106 74-75 0.5907968053569768 1106 76-77 0.6678861937645273 1106 78-79 0.776011251169674 1106 80-81 0.7009327824147142 1106 82-83 0.7341682980178561 1106 84-85 0.7811056670835939 1106 86-87 0.7587698486464944 1106 88-89 0.6745837164542046 1106 90-91 0.6254254449671173 1106 92-93 0.8635184781133098 1106 94-95 0.90508779679001 1106 96-97 0.7769021400895433 1106 98-99 0.761232183995233 1106 100-101 1.0137399008597008 1107 1 0.04671667645873967 1107 2 0.1779030181066581 1107 3 0.18988053705723473 1107 4 -0.015142056733395748 1107 5 0.09658407728741736 1107 6 0.10774606254351937 1107 7 0.09713538012907463 1107 8 0.030569865783462546 1107 9 0.04402028121944568 1107 10-11 0.12551330305861086 1107 12-13 0.0918549249688212 1107 14-15 0.11394690227822935 1107 16-17 0.11505844321672498 1107 18-19 0.19783669803915416 1107 20-21 0.22305971972070182 1107 22-23 0.2727139412588002 1107 24-25 0.11702131767096802 1107 26-27 0.0815157365433592 1107 28-29 0.15133505037719175 1107 30-31 0.22630078971207723 1107 32-33 0.3206475095215069 1107 34-35 0.20400480276902755 1107 36-37 0.16944591434145195 1107 38-39 0.19894035779019958 1107 40-41 0.16320020546173453 1107 42-43 0.26936336262742344 1107 44-45 0.23230855781108062 1107 46-47 0.27290367791638204 1107 48-49 0.34168114463573573 1107 50-51 0.30845559235920916 1107 52-53 0.18956354577115775 1107 54-55 0.2588308782077533 1107 56-57 0.2613474893176644 1107 58-59 0.2550235087035162 1107 60-61 0.1909082141779166 1107 62-63 0.07210951452439218 1107 64-65 0.09307797137359586 1107 66-67 0.17393890863281314 1107 68-69 0.3185054291515286 1107 70-71 0.1163684403417733 1107 72-73 0.012977570812441996 1107 74-75 0.30284863262247796 1107 76-77 0.32026060167518366 1107 78-79 0.3771578300169587 1107 80-81 0.3667359494444149 1107 82-83 0.2971380798523846 1107 84-85 0.37351266151708984 1107 86-87 0.3354276988003484 1107 88-89 0.297444415551805 1107 90-91 0.2357048938486308 1107 92-93 0.27289250570381185 1107 94-95 0.3418033864591301 1107 96-97 0.2010693253746112 1107 98-99 0.18633374676534586 1107 100-101 0.5960493950338837 1108 1 0.2822806101776507 1108 2 0.3516802799539427 1108 3 0.2602605335644306 1108 4 0.0251510305969731 1108 5 0.13598855424005052 1108 6 0.15713670525366297 1108 7 0.23473815245647955 1108 8 0.24070653029281175 1108 9 0.2833663735595877 1108 10-11 0.2826008697043889 1108 12-13 0.32349501893208554 1108 14-15 0.4824184410637571 1108 16-17 0.4531802564613088 1108 18-19 0.41351733249668854 1108 20-21 0.3649544459294063 1108 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-1.2864972902574188 2316 82-83 -1.4708438140052849 2316 84-85 -1.4423125811170294 2316 86-87 -1.2747068248168496 2316 88-89 -1.3115114195664326 2316 90-91 -0.9756130085279402 2316 92-93 -1.251655969874701 2316 94-95 -1.2117636000138106 2316 96-97 -1.0363441870919274 2316 98-99 -1.0378544038997148 2316 100-101 -1.2803628944906755 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 27.0 4 65.0 5 144.0 6 261.0 7 709.0 8 1588.0 9 2598.0 10 3985.0 11 6124.0 12 8400.0 13 10587.0 14 12585.0 15 15165.0 16 17814.0 17 20197.0 18 18875.0 19 17546.0 20 18083.0 21 20069.0 22 22203.0 23 25965.0 24 31901.0 25 40694.0 26 52043.0 27 67443.0 28 85037.0 29 105422.0 30 129453.0 31 156457.0 32 188431.0 33 228022.0 34 280996.0 35 349513.0 36 450309.0 37 564213.0 38 453475.0 39 70779.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.32249806269894 21.050710401249997 14.53720797379883 24.08958356225223 2 15.494387231555745 21.636652907963942 39.58804994547136 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28.205975852943492 22.018961265894283 22-23 22.79927510421851 26.663113989042014 28.016218270531418 22.521392636208066 24-25 22.633583900507826 26.82231108099729 28.027598817201767 22.51650620129312 26-27 22.591353861502515 26.991929682126713 27.913538902619106 22.503177553751673 28-29 22.577344388423818 27.0838117486483 27.905911998231907 22.432931864695977 30-31 22.91164759413111 26.859437399867208 27.778160457217403 22.45075454878427 32-33 22.775465505918657 26.997315309982024 27.620046605965577 22.607172578133742 34-35 22.894956461062172 26.96360976329527 27.79606211667305 22.34537165896951 36-37 22.887585913951312 26.859133473734826 27.77727928824898 22.476001324064878 38-39 22.844297032185924 26.86604228953874 27.74779332169936 22.541867356575978 40-41 22.865826959653887 26.767037888048616 27.84217883421234 22.524956318085152 42-43 22.849631222367563 26.822015179073727 27.580379258257086 22.747974340301624 44-45 22.764420435870285 26.87948710721544 27.60943206402474 22.746660392889538 46-47 22.933417315768924 26.782372604469334 27.394912303786327 22.889297775975415 48-49 22.884006994240924 26.89131473638359 27.3818003339507 22.842877935424788 50-51 22.9006860421676 26.950462819488223 27.246047192935368 22.902803945408813 52-53 23.005567223974126 26.890812519484996 27.307509098513123 22.79611115802776 54-55 22.769159744114873 26.841993234691763 27.37043226637826 23.018414754815105 56-57 22.838115565976345 26.936915313618613 27.18107587399428 23.043893246410754 58-59 22.805910104079206 26.887645488103363 27.39166002921915 22.91478437859828 60-61 22.881568204298176 27.008146749393042 27.314004136318676 22.79628090999011 62-63 22.837986420939924 26.95262499659955 27.276995582221165 22.93239300023936 64-65 22.59575157165939 26.89335027042977 27.257507439967473 23.25339071794337 66-67 22.639504222977592 26.88954388041792 27.220190488144947 23.250761408459546 68-69 22.65241770481779 26.904118336129212 27.281742118971213 23.161721840081782 70-71 22.654155708207558 26.70811270686138 27.285674417145266 23.35205716778579 72-73 22.697975866963958 26.825658924266072 27.146219203254386 23.330146005515587 74-75 22.66073045142913 26.998448915105488 27.00144729482722 23.339373338638158 76-77 22.623124411196795 26.81328311186929 27.0885819628485 23.47501051408541 78-79 22.563895116048545 26.88256315883401 27.066101589376945 23.487440135740496 80-81 22.620090990779705 27.00126007807221 26.94401517557907 23.434633755569013 82-83 22.708295827037546 26.84709279953894 27.064581383724924 23.38002998969859 84-85 22.670657050097592 26.855900722220483 26.889893658476822 23.583548569205103 86-87 22.515755520111906 27.014310964530598 26.900282239069778 23.569651276287722 88-89 22.561445834759894 26.728576837292543 26.945418690991303 23.764558636956266 90-91 22.529674223112078 26.722870848157683 27.018847325347345 23.728607603382894 92-93 22.631521191828487 26.600107204273904 27.01996424318946 23.74840736070815 94-95 22.422116937617627 26.636418592772905 27.051746034667612 23.88971843494186 96-97 22.379841331832505 26.532787644290195 27.030200213160384 24.057170810716915 98-99 22.193527309664056 26.540538146563 26.975486556113925 24.290447987659014 100-101 22.298511385904842 26.51172180787152 26.903460088795732 24.2863067174279 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 249.0 1 184.5 2 148.0 3 172.0 4 182.5 5 194.0 6 250.5 7 478.0 8 766.5 9 883.5 10 786.5 11 714.5 12 763.5 13 856.5 14 1013.0 15 1165.0 16 1332.0 17 1553.5 18 1793.0 19 2026.5 20 2343.5 21 2713.0 22 3223.5 23 3964.0 24 4886.0 25 6384.5 26 8722.0 27 12070.0 28 16976.0 29 24967.0 30 35758.0 31 48342.5 32 62196.0 33 73545.0 34 82394.5 35 89925.5 36 97500.5 37 104521.0 38 107218.5 39 107690.5 40 108132.5 41 109483.0 42 112203.5 43 116285.5 44 120579.0 45 125321.0 46 129430.0 47 131882.0 48 134132.5 49 134300.5 50 130788.5 51 127227.5 52 125693.0 53 123210.5 54 118740.0 55 110723.5 56 99747.5 57 88101.0 58 77898.5 59 68076.5 60 57247.5 61 47269.5 62 38671.5 63 30179.0 64 22769.5 65 17550.5 66 13494.0 67 10675.0 68 8547.5 69 6766.5 70 5302.5 71 4049.5 72 2986.0 73 2388.5 74 1926.0 75 1474.5 76 1106.0 77 748.5 78 480.0 79 309.5 80 209.5 81 154.5 82 91.5 83 43.5 84 28.0 85 18.0 86 7.5 87 4.0 88 5.0 89 2.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020361245302965552 2 0.0035085761680251374 3 0.003709887915370842 4 0.03390665001722653 5 0.001955599831358273 6 8.627646314815912E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.8758821049386375E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.2941469472223867E-4 24-25 4.60141136790182E-4 26-27 0.001998738062932353 28-29 7.189705262346593E-5 30-31 0.007146567030772513 32-33 0.023438439155249895 34-35 0.01376109587213138 36-37 6.614528841358866E-4 38-39 6.326940630865003E-4 40-41 0.0019699792418829665 42-43 0.008685163956914684 44-45 0.008282540462223275 46-47 0.007951814020155332 48-49 0.009591066819970356 50-51 0.1949991861253643 52-53 0.05071618092059287 54-55 0.01823309254531096 56-57 0.011029007872439675 58-59 0.020332486481916167 60-61 0.05410972180442046 62-63 0.09747802394689511 64-65 0.10228074706214263 66-67 0.10632136141958141 68-69 0.10442327923032191 70-71 0.09431455363146261 72-73 0.07179639674979307 74-75 0.2488788373613897 76-77 0.29955188005040845 78-79 0.007635466988612082 80-81 8.196263999075116E-4 82-83 0.0 84-85 2.8758821049386375E-5 86-87 0.0 88-89 1.5817351577162507E-4 90-91 0.006571390609784786 92-93 0.018793889555773997 94-95 0.0016680116208644096 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3477194.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.226459594346835 #Duplication Level Percentage of deduplicated Percentage of total 1 76.18320101214088 43.59694874489284 2 13.225782710264196 15.137294397450901 3 4.363403877379705 7.491064670480579 4 2.008468555043589 4.597501784468724 5 1.115969963725611 3.1931505018824193 6 0.6868290436236238 2.358287670789071 7 0.4451598208800046 1.783244435183138 8 0.3270407274123073 1.497230637837296 9 0.25349380825936824 1.3055897858194654 >10 1.2954381213981836 13.110188340824172 >50 0.06714722480351505 2.605634803772844 >100 0.026757903615328732 2.597620199356923 >500 0.0011061189248709653 0.41009295102421495 >1k 2.0111252871547283E-4 0.3161510762175018 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3975 0.11431631367131083 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.7517642098772744E-5 2 0.0 0.0 0.0 0.0 5.7517642098772744E-5 3 0.0 0.0 0.0 0.0 5.7517642098772744E-5 4 0.0 0.0 0.0 0.0 5.7517642098772744E-5 5 0.0 0.0 0.0 0.0 5.7517642098772744E-5 6 0.0 0.0 0.0 0.0 5.7517642098772744E-5 7 0.0 0.0 0.0 0.0 5.7517642098772744E-5 8 0.0 0.0 0.0 0.0 5.7517642098772744E-5 9 0.0 0.0 0.0 0.0 5.7517642098772744E-5 10-11 0.0 0.0 0.0 0.0 7.189705262346593E-5 12-13 0.0 0.0 0.0 0.0 1.7255292629631824E-4 14-15 0.0 0.0 0.0 0.0 2.013117473457046E-4 16-17 0.0 0.0 0.0 0.0 2.5882938944447734E-4 18-19 0.0 0.0 0.0 0.0 2.5882938944447734E-4 20-21 0.0 0.0 0.0 0.0 2.8758821049386374E-4 22-23 0.0 0.0 0.0 5.7517642098772744E-5 3.019676210185569E-4 24-25 0.0 0.0 0.0 2.013117473457046E-4 3.163470315432501E-4 26-27 0.0 0.0 0.0 5.320381894136479E-4 3.307264420679433E-4 28-29 0.0 0.0 0.0 0.0015529763366668643 3.451058525926365E-4 30-31 0.0 0.0 0.0 0.00622628475719215 3.451058525926365E-4 32-33 0.0 2.8758821049386372E-5 0.0 0.0213102863975953 3.594852631173297E-4 34-35 0.0 5.7517642098772744E-5 0.0 0.0426205727951906 3.7386467364202287E-4 36-37 0.0 5.7517642098772744E-5 0.0 0.07646970517031837 4.026234946914092E-4 38-39 0.0 8.627646314815912E-5 0.0 0.12881075948020157 4.026234946914092E-4 40-41 0.0 1.1503528419754549E-4 0.0 0.18956376894703028 4.026234946914092E-4 42-43 0.0 1.1503528419754549E-4 0.0 0.25289069289777905 4.170029052161024E-4 44-45 0.0 1.1503528419754549E-4 0.0 0.3312297214363076 4.4576172626548875E-4 46-47 0.0 1.1503528419754549E-4 0.0 0.4203101696367818 4.6014113679018195E-4 48-49 0.0 1.1503528419754549E-4 0.0 0.5123815352263923 4.888999578395683E-4 50-51 0.0 1.1503528419754549E-4 0.0 0.6101759062048306 5.751764209877275E-4 52-53 0.0 1.1503528419754549E-4 0.0 0.7103428799198435 6.758322946605797E-4 54-55 0.0 1.4379410524693187E-4 0.0 0.8260396170015247 7.045911157099662E-4 56-57 0.0 1.4379410524693187E-4 0.0 0.9577118791761403 7.621087578087389E-4 58-59 0.0 1.4379410524693187E-4 0.0 1.1022968520019303 7.76488168333432E-4 60-61 0.0 1.4379410524693187E-4 0.0 1.255753921121456 7.76488168333432E-4 62-63 0.0 1.4379410524693187E-4 0.0 1.4176517042189767 8.052469893828184E-4 64-65 0.0 1.4379410524693187E-4 0.0 1.59043470108369 8.196263999075116E-4 66-67 0.0 1.7255292629631824E-4 0.0 1.7890287398402276 8.340058104322048E-4 68-69 0.0 1.7255292629631824E-4 0.0 2.0087461326575395 8.340058104322048E-4 70-71 0.0 1.7255292629631824E-4 0.0 2.24377759768365 8.627646314815912E-4 72-73 0.0 1.7255292629631824E-4 0.0 2.495561075971027 9.490410946297503E-4 74-75 0.0 1.7255292629631824E-4 0.0 2.7573382445730665 0.0010353175577779094 76-77 0.0 1.7255292629631824E-4 0.0 3.054028621929061 0.0010928351998766822 78-79 0.0 1.7255292629631824E-4 0.0 3.380843864334288 0.0011215940209260684 80-81 0.0 1.7255292629631824E-4 0.0 3.7353394719995494 0.0011215940209260684 82-83 0.0 1.7255292629631824E-4 0.0 4.109707425009936 0.0012078704840742277 84-85 0.0 1.7255292629631824E-4 0.0 4.508275925933382 0.0012078704840742277 86-87 0.0 2.013117473457046E-4 0.0 4.939442550516307 0.0012222498945989209 88-89 0.0 2.013117473457046E-4 0.0 5.412381362673466 0.0012510087156483072 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3635 0.0 54.73168 1 GTATCAA 6735 0.0 39.127724 1 CAACGCA 8090 0.0 32.026146 5 ATCAACG 8205 0.0 31.53259 3 TCAACGC 8240 0.0 31.511612 4 TATCAAC 8760 0.0 29.914585 2 AACGCAG 8780 0.0 29.77952 6 GTACATG 10735 0.0 25.388618 1 ACGCAGA 10295 0.0 25.166733 7 CGCAGAG 10760 0.0 23.858635 8 TACATGG 11230 0.0 23.715435 2 ACATGGG 11120 0.0 23.437391 3 CATGGGG 8060 0.0 20.318634 4 GCAGAGT 12525 0.0 19.890337 9 GTGGTAT 1840 0.0 19.096031 1 AGAGTAC 11175 0.0 18.9386 10-11 CAGAGTA 12025 0.0 18.132637 10-11 GAGTACT 6470 0.0 17.675566 12-13 GAGTACA 8390 0.0 17.20445 1 ATGGGGG 4720 0.0 16.990467 5 >>END_MODULE