FastQCFastQC Report
Sat 18 Jun 2016
SRR1930930_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930930_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066041
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN55760.18186319100103357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.052.69591
GTATCAA50550.042.9619181
TCAACGC61000.036.070794
ATCAACG60150.035.920293
TATCAAC63300.034.22062
CAACGCA65300.033.9851575
AACGCAG70400.031.6340756
GTACATG59600.028.998491
ACGCAGA79950.027.7547287
TACATGG62700.026.7546652
CGCAGAG85000.026.451668
ACATGGG66300.025.4477543
GCAGAGT94550.022.498969
CATGGGG45450.021.3809744
TATCACG3800.021.2109072
ATCACGC3950.020.4005133
AGAGTAC85400.020.17050710-11
GTGGTAT13700.019.8609831
GAGTACA42900.019.3800181
CAGAGTA90100.019.06924210-11
Produced by FastQC (version 0.11.5)