##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930930_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3066041 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.733735132700442 30.0 16.0 31.0 2.0 33.0 2 22.496136874881973 30.0 10.0 31.0 2.0 33.0 3 23.003531590086368 30.0 16.0 31.0 2.0 34.0 4 24.98097742332865 33.0 10.0 35.0 2.0 37.0 5 24.980969921798177 33.0 10.0 35.0 2.0 37.0 6 25.818641368461805 33.0 17.0 36.0 2.0 37.0 7 25.96488305277066 33.0 17.0 36.0 2.0 37.0 8 26.86333026857762 33.0 17.0 36.0 2.0 37.0 9 28.148900161478597 35.0 18.0 38.0 2.0 39.0 10-11 28.498101949713003 35.0 25.5 38.0 2.0 39.0 12-13 28.484619905604653 35.0 25.5 38.0 2.0 39.0 14-15 28.77152686477448 36.0 24.5 39.0 2.0 40.0 16-17 27.87102537115453 34.5 17.5 38.5 2.0 40.0 18-19 27.229541777164755 34.0 16.5 38.5 2.0 40.0 20-21 26.704006893580353 34.0 11.0 38.5 2.0 40.0 22-23 26.693400544872034 34.0 15.5 38.0 2.0 40.0 24-25 26.405112488711012 33.0 11.5 38.0 2.0 40.0 26-27 26.101939928396263 33.0 10.0 38.0 2.0 40.0 28-29 26.092983590239008 33.0 10.0 38.0 2.0 40.0 30-31 26.07553584573722 33.0 10.0 38.0 2.0 40.0 32-33 25.83310203614368 33.0 9.0 38.0 2.0 40.0 34-35 22.705722460984703 30.0 2.0 37.0 2.0 40.0 36-37 21.016131225903372 22.5 2.0 36.0 2.0 39.0 38-39 20.02297490477133 20.0 2.0 35.0 2.0 39.0 40-41 21.56023011433963 26.0 2.0 35.5 2.0 39.0 42-43 21.511008822125994 26.5 2.0 36.0 2.0 39.0 44-45 19.410178631009828 20.0 2.0 35.0 2.0 38.5 46-47 20.406441401142388 22.0 2.0 34.5 2.0 38.0 48-49 19.824000885832902 23.5 2.0 35.0 2.0 38.0 50-51 17.61781675457047 12.0 2.0 33.5 2.0 37.5 52-53 20.798640657447176 24.5 2.0 34.5 2.0 38.0 54-55 22.698900145170924 30.0 2.0 37.0 2.0 39.0 56-57 22.99168699961938 31.0 2.0 37.0 2.0 39.0 58-59 22.62372600366401 30.0 2.0 37.0 2.0 39.0 60-61 22.346668390931498 29.5 2.0 36.5 2.0 39.0 62-63 21.888283783550186 28.5 2.0 36.0 2.0 39.0 64-65 21.399455682425643 28.0 2.0 35.5 2.0 39.0 66-67 20.99135008305499 28.0 2.0 35.0 2.0 38.0 68-69 20.5440568146349 27.0 2.0 34.5 2.0 38.0 70-71 19.944760034193934 26.0 2.0 34.0 2.0 37.5 72-73 19.493403708560976 26.0 2.0 34.0 2.0 37.0 74-75 18.958880686853178 25.0 2.0 34.0 2.0 36.0 76-77 18.395890661605634 23.0 2.0 33.0 2.0 36.0 78-79 17.90995912970505 21.5 2.0 33.0 2.0 35.0 80-81 17.408093205537696 20.0 2.0 32.0 2.0 35.0 82-83 16.936367778513073 18.0 2.0 32.0 2.0 35.0 84-85 16.412620542256285 14.5 2.0 31.5 2.0 34.5 86-87 15.844121784411886 7.5 2.0 31.0 2.0 34.0 88-89 15.2829212003362 4.0 2.0 31.0 2.0 34.0 90-91 14.699133671076153 2.0 2.0 31.0 2.0 34.0 92-93 14.046407402901657 2.0 2.0 30.0 2.0 34.0 94-95 13.361073938672053 2.0 2.0 29.5 2.0 34.0 96-97 12.592944777972637 2.0 2.0 29.0 2.0 34.0 98-99 11.721778345429822 2.0 2.0 29.0 2.0 34.0 100-101 10.492751727716623 2.0 2.0 25.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.773481323298071 1101 2 8.093672437516922 1101 3 7.556119601182456 1101 4 9.513589070802798 1101 5 9.299047967526267 1101 6 8.67753643581674 1101 7 8.37210007468872 1101 8 7.31288777871411 1101 9 7.47886676990878 1101 10-11 6.902893259391593 1101 12-13 6.7117842641381635 1101 14-15 7.276602563172645 1101 16-17 8.010872039356808 1101 18-19 8.557720492478438 1101 20-21 9.154222108262392 1101 22-23 9.009508465708613 1101 24-25 8.892354093242602 1101 26-27 8.98967348273008 1101 28-29 8.798056004287773 1101 30-31 8.655217624979258 1101 32-33 8.485857056664031 1101 34-35 11.144954859577908 1101 36-37 5.187530957628621 1101 38-39 8.558829155626984 1101 40-41 10.223349331862224 1101 42-43 10.593512958515266 1101 44-45 4.39807627710017 1101 46-47 6.148893547948468 1101 48-49 9.694354784672651 1101 50-51 -4.467914200043671 1101 52-53 5.9524914858551305 1101 54-55 8.492598864138944 1101 56-57 9.162020498638277 1101 58-59 9.41040169969937 1101 60-61 9.072119128162964 1101 62-63 9.333023952412503 1101 64-65 9.275178594938236 1101 66-67 9.147203779466071 1101 68-69 9.292844981247999 1101 70-71 9.272934677542956 1101 72-73 9.16786364528065 1101 74-75 9.221322140696842 1101 76-77 9.096744801585949 1101 78-79 9.168074553614389 1101 80-81 8.958774254580188 1101 82-83 8.77968117862721 1101 84-85 8.7563965035821 1101 86-87 8.545794560188549 1101 88-89 8.460606025302724 1101 90-91 8.368489300762342 1101 92-93 8.505855741822968 1101 94-95 8.278003570106621 1101 96-97 7.9162331953756695 1101 98-99 7.822682381948068 1101 100-101 7.20669338457731 1102 1 6.5021655407843575 1102 2 7.63534002985973 1102 3 7.482194469868684 1102 4 9.293027846582884 1102 5 9.192378338695956 1102 6 8.391283308353785 1102 7 8.080372128261569 1102 8 7.211780458467192 1102 9 7.075505348916561 1102 10-11 6.7324460784721545 1102 12-13 6.324977529447526 1102 14-15 6.825626794729203 1102 16-17 7.735557706187446 1102 18-19 8.153976846583944 1102 20-21 8.587036269520471 1102 22-23 8.347910642898896 1102 24-25 8.473812218298804 1102 26-27 8.525830815936462 1102 28-29 8.353789959675467 1102 30-31 7.998485473337638 1102 32-33 8.11234911436275 1102 34-35 10.634319158100531 1102 36-37 0.6852445904578595 1102 38-39 3.1596606243840952 1102 40-41 8.393875274597256 1102 42-43 9.630760579527585 1102 44-45 0.7614356351569924 1102 46-47 1.3313838812447756 1102 48-49 7.7041516119838 1102 50-51 -5.7599403728753416 1102 52-53 4.464674759151752 1102 54-55 7.308231947275242 1102 56-57 7.9983221844267725 1102 58-59 8.255688632854973 1102 60-61 8.265247278767827 1102 62-63 8.26182443482201 1102 64-65 8.175540454965425 1102 66-67 8.142861776514486 1102 68-69 7.9986746779964975 1102 70-71 7.954574340914217 1102 72-73 7.7893543012412145 1102 74-75 7.823946829272941 1102 76-77 7.70316571446131 1102 78-79 7.767470219334225 1102 80-81 7.708809906335535 1102 82-83 7.541633403159214 1102 84-85 7.571211341906146 1102 86-87 7.471590139159302 1102 88-89 7.3015696806126655 1102 90-91 7.196496558063284 1102 92-93 7.226382795369366 1102 94-95 6.950202077387727 1102 96-97 6.770262266896053 1102 98-99 6.625963983209102 1102 100-101 6.300162131106028 1103 1 6.398586747525162 1103 2 7.7521702326445165 1103 3 7.326698410310389 1103 4 8.988335450233631 1103 5 9.103796274714394 1103 6 8.158301032554014 1103 7 7.925842333538331 1103 8 7.054110458778691 1103 9 7.151756478094523 1103 10-11 6.439529684365684 1103 12-13 6.3367902883797065 1103 14-15 6.959224433579159 1103 16-17 7.653652588285691 1103 18-19 8.171647936513232 1103 20-21 8.622902076454398 1103 22-23 8.427940235148839 1103 24-25 8.466446240041321 1103 26-27 8.278198507429469 1103 28-29 8.148998195665875 1103 30-31 8.01962399621712 1103 32-33 7.780990674341567 1103 34-35 10.503870134644778 1103 36-37 -3.5374925369134154 1103 38-39 -2.1567543524093544 1103 40-41 6.88739846557629 1103 42-43 9.123881160158678 1103 44-45 0.4370742047610783 1103 46-47 -0.033529522486958285 1103 48-49 7.537351001448961 1103 50-51 -6.1514607915278035 1103 52-53 4.131574114352201 1103 54-55 7.300600261762984 1103 56-57 7.759212599652759 1103 58-59 7.966252443090777 1103 60-61 7.972472148717674 1103 62-63 7.892591652839016 1103 64-65 7.714047603830014 1103 66-67 7.7923518553233215 1103 68-69 8.082348945198937 1103 70-71 7.9855572708584575 1103 72-73 7.910794197824085 1103 74-75 7.711939384485849 1103 76-77 7.52974921133076 1103 78-79 7.361949707232551 1103 80-81 7.230280796906509 1103 82-83 7.050332278653716 1103 84-85 7.15461694262882 1103 86-87 6.977353151961845 1103 88-89 7.03623435369618 1103 90-91 6.80577995836855 1103 92-93 6.967701207978779 1103 94-95 6.5939690150571035 1103 96-97 6.272901428345138 1103 98-99 6.086020052735316 1103 100-101 5.70179266407802 1104 1 6.187158215457298 1104 2 7.510541734287941 1104 3 7.005376344327022 1104 4 8.801783985413305 1104 5 8.788035761006217 1104 6 7.983750705489683 1104 7 7.802636892406671 1104 8 6.756545532133156 1104 9 6.923271330510392 1104 10-11 6.144563201166225 1104 12-13 5.779647425448513 1104 14-15 6.534997848338222 1104 16-17 7.226562772012219 1104 18-19 7.637855813609654 1104 20-21 8.007165184959366 1104 22-23 7.78751043242471 1104 24-25 7.812160976088421 1104 26-27 8.055928676115567 1104 28-29 7.937265775778812 1104 30-31 7.680691428519012 1104 32-33 7.470983972602198 1104 34-35 10.210160126281785 1104 36-37 -3.8070392092202425 1104 38-39 -2.3347534010378865 1104 40-41 6.276536947660688 1104 42-43 8.719890464798521 1104 44-45 0.024422098777971968 1104 46-47 -0.726961007534058 1104 48-49 7.013919832445193 1104 50-51 -6.312291596514481 1104 52-53 3.468245959267307 1104 54-55 6.730097274748314 1104 56-57 7.34347967895857 1104 58-59 7.651699579576118 1104 60-61 7.499557872635187 1104 62-63 7.484926143377166 1104 64-65 7.527016198847228 1104 66-67 7.33282262212548 1104 68-69 7.316243701148657 1104 70-71 7.408015026399749 1104 72-73 7.29160534329478 1104 74-75 7.182626568037335 1104 76-77 6.99857092885609 1104 78-79 6.78024156595632 1104 80-81 6.7110112702908005 1104 82-83 6.5648147617494494 1104 84-85 6.424809883031152 1104 86-87 6.331032382566507 1104 88-89 6.120387981549484 1104 90-91 5.884047886939516 1104 92-93 5.636063163492841 1104 94-95 5.6624616828518 1104 96-97 5.365431420046976 1104 98-99 5.141715396687706 1104 100-101 4.715094036516451 1105 1 6.0080306586717995 1105 2 7.044399984476662 1105 3 6.681854626917321 1105 4 8.576244339971037 1105 5 8.584568311511543 1105 6 7.769877341919077 1105 7 7.567590610629562 1105 8 6.424332536673472 1105 9 6.454512121039546 1105 10-11 6.064679364605073 1105 12-13 5.874679561084417 1105 14-15 6.166754152541131 1105 16-17 6.937304373762789 1105 18-19 7.629989497303434 1105 20-21 8.033396663580792 1105 22-23 7.752521239517122 1105 24-25 7.739795564378774 1105 26-27 7.82653114886342 1105 28-29 7.590089901873895 1105 30-31 7.512916834602507 1105 32-33 7.212982912380333 1105 34-35 9.864885638742052 1105 36-37 -4.0148891356232745 1105 38-39 -2.5483286633945355 1105 40-41 5.996708308826385 1105 42-43 8.312344342586577 1105 44-45 -3.7686378409891343 1105 46-47 -4.7915929754098485 1105 48-49 5.465671414701234 1105 50-51 -10.743956042401797 1105 52-53 0.5644579906382141 1105 54-55 5.3687380770183175 1105 56-57 6.566171233982153 1105 58-59 6.8844240404822585 1105 60-61 6.893836693255576 1105 62-63 6.940766287450721 1105 64-65 6.939900662243371 1105 66-67 6.6001085034058455 1105 68-69 6.358870648040643 1105 70-71 6.418283586389162 1105 72-73 6.435424576846174 1105 74-75 6.337722281526066 1105 76-77 6.309695171808038 1105 78-79 6.269386505494911 1105 80-81 6.1605331756328034 1105 82-83 6.058928087686315 1105 84-85 5.983399699306641 1105 86-87 5.859127867936154 1105 88-89 5.9539609745349225 1105 90-91 5.942528568523095 1105 92-93 5.692829159801965 1105 94-95 5.417100202748985 1105 96-97 5.240198129868469 1105 98-99 4.9684029066359034 1105 100-101 4.485709264627655 1106 1 5.836834709014504 1106 2 7.17259581472527 1106 3 6.640956195947485 1106 4 8.183209209934976 1106 5 8.24317008353648 1106 6 7.5116310256771115 1106 7 7.2458955804191625 1106 8 6.268185711750494 1106 9 6.128958757296804 1106 10-11 5.6585811628999245 1106 12-13 5.483536628884668 1106 14-15 5.892268445761104 1106 16-17 6.507759082047368 1106 18-19 6.988470905370683 1106 20-21 7.486998746068313 1106 22-23 7.011895633518037 1106 24-25 7.283165564139956 1106 26-27 7.376242323128462 1106 28-29 7.18627651377178 1106 30-31 6.806159019303863 1106 32-33 6.819703710985635 1106 34-35 9.440788201252502 1106 36-37 -0.41274040016127955 1106 38-39 0.545134045272146 1106 40-41 6.735700165153851 1106 42-43 8.124716802166734 1106 44-45 -3.8468207750994203 1106 46-47 -4.888408488986617 1106 48-49 4.8894449203015 1106 50-51 -10.733318734351263 1106 52-53 0.17805687297289197 1106 54-55 4.920329508250855 1106 56-57 5.960319682863293 1106 58-59 6.493105768564792 1106 60-61 6.219028391963576 1106 62-63 6.289430438574357 1106 64-65 6.317436640081123 1106 66-67 6.152467780266829 1106 68-69 5.871624021336082 1106 70-71 5.970130313397018 1106 72-73 5.879630724005441 1106 74-75 5.893931819892082 1106 76-77 5.634128816400022 1106 78-79 5.363438949790758 1106 80-81 5.203018786885881 1106 82-83 5.3880023837721005 1106 84-85 5.348332042343923 1106 86-87 5.064892202949169 1106 88-89 5.166769180299973 1106 90-91 4.933574731921944 1106 92-93 4.919776166226148 1106 94-95 4.787644228732317 1106 96-97 4.402705043631512 1106 98-99 4.0191447211703295 1106 100-101 3.869242806528163 1107 1 6.000135562667289 1107 2 7.369894121568642 1107 3 6.951239646402609 1107 4 8.114311412453485 1107 5 8.601098112588968 1107 6 7.21587702860122 1107 7 7.278621892733458 1107 8 6.49187556695966 1107 9 6.541332289344151 1107 10-11 6.000681337403531 1107 12-13 5.751338732360598 1107 14-15 6.042515580600508 1107 16-17 7.017550149349159 1107 18-19 7.313943149864386 1107 20-21 7.756572391307191 1107 22-23 7.61796911368593 1107 24-25 7.728633092319939 1107 26-27 7.789812761274003 1107 28-29 7.630152757699527 1107 30-31 7.643458929693907 1107 32-33 7.179467895299183 1107 34-35 9.832263558513262 1107 36-37 -0.2188911335480661 1107 38-39 0.7452706297177443 1107 40-41 7.248808404553937 1107 42-43 8.763301342090333 1107 44-45 -3.478825963044521 1107 46-47 -4.548175220109572 1107 48-49 5.47364536363499 1107 50-51 -10.60474145755081 1107 52-53 0.5420531942481794 1107 54-55 5.576898484339143 1107 56-57 6.7377423117365645 1107 58-59 7.114226364706848 1107 60-61 6.944554402433791 1107 62-63 6.9447832660044355 1107 64-65 6.9856909437173975 1107 66-67 6.837543477102184 1107 68-69 6.673791544799741 1107 70-71 6.788688630616278 1107 72-73 6.816008406408873 1107 74-75 6.875470658735637 1107 76-77 6.760273229926408 1107 78-79 6.65294100138723 1107 80-81 6.420488012938794 1107 82-83 6.257940222757146 1107 84-85 6.1475546995150765 1107 86-87 5.822442077966857 1107 88-89 5.9060656006178505 1107 90-91 5.732681650754184 1107 92-93 5.472958640404334 1107 94-95 5.340835286601488 1107 96-97 5.230367544221048 1107 98-99 5.153489766682759 1107 100-101 4.902518758044458 1108 1 6.047964866703296 1108 2 6.695627938252063 1108 3 6.703291770820371 1108 4 8.231473695981979 1108 5 8.427313667031871 1108 6 7.6736592831339365 1108 7 7.519143032327602 1108 8 6.5772353145996725 1108 9 5.0797497317807405 1108 10-11 5.90406840486904 1108 12-13 5.266758916192249 1108 14-15 6.111250146363275 1108 16-17 6.286492182635474 1108 18-19 7.0441817701988185 1108 20-21 7.884450888808306 1108 22-23 7.812164337095908 1108 24-25 7.423149256353923 1108 26-27 7.710481925743327 1108 28-29 7.739213414823823 1108 30-31 7.4994650948913915 1108 32-33 7.400960066570349 1108 34-35 9.898138092299618 1108 36-37 -3.947678004135131 1108 38-39 -2.4926895213531246 1108 40-41 5.976855346859139 1108 42-43 8.267778823301253 1108 44-45 -3.5394883165132303 1108 46-47 -4.6701820075789 1108 48-49 5.508450426522359 1108 50-51 -10.751167512438847 1108 52-53 0.6245551976759067 1108 54-55 5.558389818942295 1108 56-57 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2.7878005003240744 1109 18-19 3.8902359464298435 1109 20-21 4.4547489914656175 1109 22-23 5.759135165228152 1109 24-25 5.901143107293976 1109 26-27 4.661334903750451 1109 28-29 4.501008348385966 1109 30-31 4.283838877571959 1109 32-33 4.2214212527031805 1109 34-35 6.825171260459086 1109 36-37 -5.134831472487647 1109 38-39 -3.8982544007045323 1109 40-41 3.002699183564637 1109 42-43 3.986934810737683 1109 44-45 -5.116279895499716 1109 46-47 -6.735192754829708 1109 48-49 2.0239249413697564 1109 50-51 -11.057794972709853 1109 52-53 -1.5506409172543094 1109 54-55 2.406999909744787 1109 56-57 3.9803361065267744 1109 58-59 4.702890776585097 1109 60-61 4.690948811413406 1109 62-63 4.751805836596812 1109 64-65 4.296861763976832 1109 66-67 4.518078161171328 1109 68-69 4.419909448327395 1109 70-71 4.616175610148197 1109 72-73 4.302828981086048 1109 74-75 4.354019779862391 1109 76-77 4.548957277699067 1109 78-79 4.709217541569348 1109 80-81 4.46297234095708 1109 82-83 4.420337752107468 1109 84-85 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-10.384844369342831 2313 98-99 -9.440139466199653 2313 100-101 -8.193510355758725 2314 1 -21.522549906370113 2314 2 -20.278481108351652 2314 3 -20.7636591886679 2314 4 -22.71217540544964 2314 5 -22.71006068569429 2314 6 -23.55083051278443 2314 7 -23.691335188811603 2314 8 -24.5921219805572 2314 9 -25.857502781164737 2314 10-11 -26.222188067869308 2314 12-13 -26.218924909576867 2314 14-15 -26.473885400392746 2314 16-17 -25.580702456414176 2314 18-19 -24.953990633090854 2314 20-21 -24.442232023162457 2314 22-23 -24.403796674174274 2314 24-25 -24.123911358936965 2314 26-27 -23.826526907640773 2314 28-29 -23.820031178535913 2314 30-31 -23.796148673003827 2314 32-33 -23.55106551978359 2314 34-35 -20.559827183362884 2314 36-37 -18.843048092468972 2314 38-39 -17.884404416266314 2314 40-41 -19.40937675792307 2314 42-43 -19.370052428602495 2314 44-45 -17.271897698176343 2314 46-47 -18.193331565909695 2314 48-49 -17.680401233544654 2314 50-51 -15.448395657428186 2314 52-53 -18.58394312702711 2314 54-55 -20.435670491749146 2314 56-57 -20.71029570175209 2314 58-59 -20.332278846819822 2314 60-61 -20.05881776188258 2314 62-63 -19.60549263834872 2314 64-65 -19.11210182145734 2314 66-67 -18.704455296656246 2314 68-69 -18.280837517125455 2314 70-71 -17.68510045583375 2314 72-73 -17.24836927599456 2314 74-75 -16.70776048780023 2314 76-77 -16.158948106676903 2314 78-79 -15.69025335790155 2314 80-81 -15.181596597729506 2314 82-83 -14.717228385458672 2314 84-85 -14.187975649963771 2314 86-87 -13.632800104743138 2314 88-89 -13.076153896623104 2314 90-91 -12.509194498847286 2314 92-93 -11.87345460300462 2314 94-95 -11.211586540498454 2314 96-97 -10.444371879445411 2314 98-99 -9.586393740368132 2314 100-101 -8.376911039625682 2315 1 -21.522549906370113 2315 2 -20.278481108351652 2315 3 -20.7636591886679 2315 4 -16.66955044378144 2315 5 -22.71006068569429 2315 6 -23.55083051278443 2315 7 -23.691335188811603 2315 8 -24.5921219805572 2315 9 -20.29663188266734 2315 10-11 -18.607652649286052 2315 12-13 -14.439256096329396 2315 14-15 -14.217982303183302 2315 16-17 -20.648013097321872 2315 18-19 -24.953990633090854 2315 20-21 -24.442232023162457 2315 22-23 -24.403796674174274 2315 24-25 -24.123911358936965 2315 26-27 -23.826526907640773 2315 28-29 -23.820031178535913 2315 30-31 -23.796148673003827 2315 32-33 -23.55106551978359 2315 34-35 -20.559827183362884 2315 36-37 -18.843048092468972 2315 38-39 -17.884404416266314 2315 40-41 -19.40937675792307 2315 42-43 -19.370052428602495 2315 44-45 -17.271897698176343 2315 46-47 -18.193331565909695 2315 48-49 -17.680401233544654 2315 50-51 -15.448395657428186 2315 52-53 -18.58394312702711 2315 54-55 -20.435670491749146 2315 56-57 -20.71029570175209 2315 58-59 -20.332278846819822 2315 60-61 -20.05881776188258 2315 62-63 -19.60549263834872 2315 64-65 -19.11210182145734 2315 66-67 -18.704455296656246 2315 68-69 -18.280837517125455 2315 70-71 -17.68510045583375 2315 72-73 -17.24836927599456 2315 74-75 -16.70776048780023 2315 76-77 -16.158948106676903 2315 78-79 -15.69025335790155 2315 80-81 -15.181596597729506 2315 82-83 -14.717228385458672 2315 84-85 -14.187975649963771 2315 86-87 -13.632800104743138 2315 88-89 -13.076153896623104 2315 90-91 -12.509194498847286 2315 92-93 -11.87345460300462 2315 94-95 -11.211586540498454 2315 96-97 -10.444371879445411 2315 98-99 -9.586393740368132 2315 100-101 -8.376911039625682 2316 1 -21.522549906370113 2316 2 -20.278481108351652 2316 3 -20.7636591886679 2316 4 -22.71217540544964 2316 5 -22.71006068569429 2316 6 -23.55083051278443 2316 7 -23.691335188811603 2316 8 -24.5921219805572 2316 9 -25.857502781164737 2316 10-11 -26.222188067869308 2316 12-13 -26.218924909576867 2316 14-15 -26.473885400392746 2316 16-17 -25.580702456414176 2316 18-19 -24.953990633090854 2316 20-21 -24.442232023162457 2316 22-23 -24.403796674174274 2316 24-25 -24.123911358936965 2316 26-27 -23.826526907640773 2316 28-29 -23.820031178535913 2316 30-31 -23.796148673003827 2316 32-33 -23.55106551978359 2316 34-35 -20.559827183362884 2316 36-37 -18.843048092468972 2316 38-39 -17.884404416266314 2316 40-41 -19.40937675792307 2316 42-43 -19.370052428602495 2316 44-45 -17.271897698176343 2316 46-47 -18.193331565909695 2316 48-49 -17.680401233544654 2316 50-51 -15.448395657428186 2316 52-53 -18.58394312702711 2316 54-55 -20.435670491749146 2316 56-57 -20.71029570175209 2316 58-59 -20.332278846819822 2316 60-61 -20.05881776188258 2316 62-63 -19.60549263834872 2316 64-65 -19.11210182145734 2316 66-67 -18.704455296656246 2316 68-69 -18.280837517125455 2316 70-71 -17.68510045583375 2316 72-73 -17.24836927599456 2316 74-75 -16.70776048780023 2316 76-77 -16.158948106676903 2316 78-79 -15.69025335790155 2316 80-81 -15.181596597729506 2316 82-83 -14.717228385458672 2316 84-85 -14.187975649963771 2316 86-87 -13.632800104743138 2316 88-89 -13.076153896623104 2316 90-91 -12.509194498847286 2316 92-93 -11.87345460300462 2316 94-95 -11.211586540498454 2316 96-97 -10.444371879445411 2316 98-99 -9.586393740368132 2316 100-101 -8.376911039625682 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 461294.0 3 26041.0 4 60280.0 5 29638.0 6 23762.0 7 29274.0 8 38653.0 9 48225.0 10 52799.0 11 51853.0 12 48290.0 13 45423.0 14 43203.0 15 46451.0 16 50823.0 17 54283.0 18 56109.0 19 58890.0 20 62164.0 21 66379.0 22 71739.0 23 77042.0 24 83174.0 25 89764.0 26 96711.0 27 105562.0 28 114523.0 29 124164.0 30 132510.0 31 137193.0 32 134887.0 33 134639.0 34 132243.0 35 120545.0 36 96127.0 37 51911.0 38 9348.0 39 125.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99587128417483 19.704221880896736 13.421988940749 23.877917894179433 2 15.193112969518799 21.009165507181468 41.058364517218614 22.739357006081118 3 18.173060474121655 26.78036063265623 30.584520371811273 24.46205852141084 4 12.158994368696973 17.277408262526524 39.689682929405265 30.873914439371237 5 12.589243857317367 38.008935727619935 35.868091319842925 13.53372909521977 6 29.414745869325575 38.13400325097934 18.867046155187552 13.584204724507526 7 25.61922645595722 32.95350143454369 24.036294934350384 17.390977175148706 8 24.598841367392833 35.93887728656245 21.754331140004965 17.70795020603975 9 25.833264092989054 16.35950304382796 21.628351246679404 36.178881616503574 10-11 24.056532823343215 26.624014322532684 29.778164768454136 19.541288085669965 12-13 24.567808537892542 24.72748320751905 29.57095382441977 21.133754430168633 14-15 21.807534648355293 26.08857764168985 27.3072721855111 24.79661552444376 16-17 21.521354655660833 28.962184901839166 27.797799521641554 21.718660920858447 18-19 21.40284388800204 28.05122299563437 29.673204287534073 20.87272882882952 20-21 22.175532283539244 27.368002532622004 29.51884981486249 20.937615368976264 22-23 21.85559364110421 27.28671322381385 29.345562767074597 21.51213036800734 24-25 21.65358959187751 27.397341986820933 29.361731289519582 21.587337131781972 26-27 21.63281733966777 27.561833810866755 29.196762962936567 21.608585886528907 28-29 21.71376676565399 27.66090254459259 29.200292066690686 21.42503862306273 30-31 21.98247845801337 27.431679910280003 29.139447718117058 21.446393913589567 32-33 21.73538802449427 27.554433778503178 29.062857406657294 21.647320790345255 34-35 22.16246096329959 27.573179481035393 28.89621980685357 21.368139748811448 36-37 22.653145551509347 27.486476524912074 28.44952129880283 21.410856624775754 38-39 22.348605346180044 27.64064240348212 28.488288742245444 21.522463508092393 40-41 22.345663851668565 27.555576642679174 28.656244754522625 21.442514751129636 42-43 22.45044379479265 27.66546346266531 28.187808210198682 21.696284532343356 44-45 22.621874066412413 27.805322828994665 28.09134956118986 21.48145354340306 46-47 22.080376003399568 27.757775849625588 28.297519164209024 21.864328982765823 48-49 22.518397876704064 27.911086982748223 27.72918824699123 21.841326893556477 50-51 22.887258890061656 27.981656506675108 27.559688224406848 21.57139637885639 52-53 21.970275213635297 28.036059420152075 28.074177550315287 21.919487815897337 54-55 21.675961228754844 28.022917431330104 28.30853326941371 21.992588070501345 56-57 21.76242859034354 28.21696580027513 28.03366619508717 21.986939414294167 58-59 21.804866467053458 28.077080345887357 28.17370871671569 21.944344470343495 60-61 21.804186063835143 28.12666634100199 28.191836709484086 21.87731088567878 62-63 21.822898630925287 28.04692131707004 28.147215239437944 21.98296481256673 64-65 21.59389076416592 27.95079576149507 28.148040809058916 22.3072726652801 66-67 21.541223379056376 28.117590843848195 28.126712663426407 22.21447311366902 68-69 21.76495239678104 28.000457992619854 28.09530909143552 22.139280519163588 70-71 21.678161621376084 27.850226059733057 27.987795457341925 22.483816861548934 72-73 21.65151003280799 27.994259566149736 27.94559594201589 22.408634459026384 74-75 21.70022009381108 28.142183911638863 27.804945767193335 22.352650227356722 76-77 21.638222681724777 28.032719934214278 27.84750442894645 22.481552955114488 78-79 21.636918338362037 28.031823145436576 27.791447238551232 22.53981127765016 80-81 21.685446864745987 28.123030961735108 27.686918435020857 22.504603738498044 82-83 21.811701123149245 28.062597078907153 27.722928781941675 22.402773016001927 84-85 21.763557058078327 28.069342345565595 27.645904660355853 22.521195936000225 86-87 21.60712273188324 28.105379494879358 27.611983436521815 22.675514336715587 88-89 21.631929341426858 27.987229888783304 27.536914468513846 22.843926301275996 90-91 21.65092089385262 28.007719704835658 27.58589733668747 22.75546206462425 92-93 21.685870387010926 27.814688384675918 27.676483316568955 22.822957911744197 94-95 21.458610671864083 27.816735924686498 27.714503732578237 23.01014967087118 96-97 21.54804282832629 27.590740165478067 27.56565830641287 23.29555869978277 98-99 21.354234203170012 27.69508316601022 27.540173071212255 23.41050955960751 100-101 21.559656514233886 27.80691360191931 27.439619356915063 23.193810526931742 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 38063.0 1 40574.5 2 30128.0 3 18705.5 4 24192.5 5 29958.0 6 31485.0 7 29666.5 8 26860.5 9 24167.5 10 21318.0 11 18501.5 12 15768.0 13 13327.0 14 11483.0 15 10122.0 16 9164.5 17 8736.0 18 8679.5 19 9006.5 20 9815.0 21 11169.5 22 12826.0 23 14930.5 24 17845.5 25 21299.5 26 25678.0 27 31054.5 28 37101.0 29 44599.5 30 52448.5 31 59354.5 32 66348.0 33 71787.0 34 75407.0 35 78684.0 36 82525.5 37 86422.0 38 88819.5 39 90273.0 40 91312.0 41 92129.0 42 92859.5 43 93519.5 44 93985.0 45 94461.0 46 94158.0 47 92817.0 48 90885.5 49 88381.5 50 84694.5 51 80411.5 52 76764.0 53 72462.0 54 67369.0 55 61402.5 56 54198.0 57 47572.0 58 41611.0 59 35547.0 60 29537.5 61 24251.5 62 19765.0 63 15534.0 64 11988.5 65 9107.0 66 6848.0 67 5281.0 68 4074.5 69 3217.0 70 2594.0 71 1962.0 72 1495.5 73 1216.5 74 962.5 75 745.5 76 558.0 77 375.5 78 248.0 79 172.0 80 113.5 81 76.0 82 49.0 83 21.0 84 13.0 85 12.0 86 7.5 87 3.5 88 2.5 89 2.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.510145167660838 2 21.4961900379023 3 17.255313937419622 4 22.488414212334405 5 22.357333121116124 6 16.208817820766257 7 15.04807013343918 8 12.121755710376998 9 3.0431426063774096 10-11 6.543487187549026 12-13 3.5886832563556714 14-15 7.452493296730213 16-17 11.554542160395116 18-19 14.637084109442764 20-21 15.056077201837809 22-23 12.973701264921115 24-25 15.52322685834925 26-27 13.04889595409846 28-29 13.05303810353482 30-31 13.917573183137472 32-33 12.394648342928226 34-35 19.285016084259798 36-37 23.987415693397445 38-39 23.104550787155162 40-41 18.197016934868124 42-43 18.150164984747434 44-45 23.46941870640347 46-47 12.848409398308764 48-49 21.598895774714038 50-51 21.265110283913362 52-53 11.313041802115496 54-55 12.916363479809956 56-57 12.429465228938556 58-59 13.45631059728164 60-61 12.869413683639586 62-63 12.830764494016877 64-65 13.908196270043355 66-67 12.22071068195109 68-69 13.261711112147553 70-71 11.864436907399476 72-73 11.22931819894124 74-75 11.309242113853012 76-77 11.354251296704772 78-79 11.770977622282285 80-81 11.744396112119832 82-83 10.244204170785713 84-85 10.161719950907374 86-87 10.287240124968974 88-89 11.70690476741831 90-91 9.17207238911678 92-93 11.21964774769809 94-95 11.309079037103547 96-97 10.860585360730662 98-99 10.708402138131877 100-101 10.819196481716977 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3066041.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.01670155203833 #Duplication Level Percentage of deduplicated Percentage of total 1 98.91304682554463 95.96217543476656 2 0.7606011879214238 1.4758203689739722 3 0.15402193105172923 0.4482809915194273 4 0.06273669287166832 0.24346028034770145 5 0.027688211157906092 0.13431094592081966 6 0.01569580821073074 0.09136533244790981 7 0.012707476361735191 0.08629862091662316 8 0.00929666827140091 0.0721545672891845 9 0.0050380126838442405 0.043989423566751035 >10 0.035176546403456785 0.5790518495851991 >50 0.002023505748866969 0.14046818320439264 >100 0.0018325729891599649 0.37739395772338985 >500 0.0 0.0 >1k 1.0092058754540345E-4 0.1632483152559617 >5k 3.3640195848467804E-5 0.18198172848205563 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5576 0.18186319100103357 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2615349892581344E-5 2 0.0 0.0 0.0 0.0 3.2615349892581344E-5 3 0.0 0.0 0.0 0.0 3.2615349892581344E-5 4 0.0 0.0 0.0 0.0 3.2615349892581344E-5 5 0.0 0.0 0.0 0.0 3.2615349892581344E-5 6 0.0 0.0 0.0 0.0 3.2615349892581344E-5 7 0.0 0.0 0.0 3.2615349892581344E-5 3.2615349892581344E-5 8 0.0 0.0 0.0 3.2615349892581344E-5 3.2615349892581344E-5 9 0.0 0.0 0.0 3.2615349892581344E-5 3.2615349892581344E-5 10-11 0.0 0.0 0.0 3.2615349892581344E-5 9.784604967774404E-5 12-13 0.0 0.0 0.0 3.2615349892581344E-5 1.793844244091974E-4 14-15 0.0 0.0 0.0 3.2615349892581344E-5 2.2830744924806943E-4 16-17 0.0 0.0 0.0 3.2615349892581344E-5 2.6092279914065075E-4 18-19 0.0 0.0 0.0 3.2615349892581344E-5 2.6092279914065075E-4 20-21 0.0 0.0 0.0 9.784604967774404E-5 2.9353814903323213E-4 22-23 0.0 0.0 0.0 1.793844244091974E-4 3.587688488183948E-4 24-25 0.0 0.0 3.2615349892581344E-5 2.283074492480694E-4 3.9138419871097616E-4 26-27 0.0 0.0 3.2615349892581344E-5 3.424611738721042E-4 3.9138419871097616E-4 28-29 0.0 0.0 3.2615349892581344E-5 7.664607224756616E-4 3.9138419871097616E-4 30-31 0.0 0.0 3.2615349892581344E-5 0.00290276614043974 3.9138419871097616E-4 32-33 0.0 0.0 3.2615349892581344E-5 0.008365837247447116 3.9138419871097616E-4 34-35 0.0 0.0 3.2615349892581344E-5 0.01601413679725744 3.9138419871097616E-4 36-37 0.0 1.6307674946290672E-5 3.2615349892581344E-5 0.027739355083640435 3.9138419871097616E-4 38-39 0.0 3.2615349892581344E-5 3.2615349892581344E-5 0.04787933364230942 3.9138419871097616E-4 40-41 0.0 3.2615349892581344E-5 3.2615349892581344E-5 0.07000884854442585 3.9138419871097616E-4 42-43 0.0 3.2615349892581344E-5 3.2615349892581344E-5 0.09145344109879809 4.239995486035575E-4 44-45 0.0 3.2615349892581344E-5 3.2615349892581344E-5 0.11780664381200381 4.239995486035575E-4 46-47 0.0 3.2615349892581344E-5 3.2615349892581344E-5 0.15063399347888695 4.4030722354984815E-4 48-49 0.0 3.2615349892581344E-5 3.2615349892581344E-5 0.18662503208535045 4.566148984961388E-4 50-51 0.0 6.523069978516269E-5 3.2615349892581344E-5 0.22967729394355785 4.566148984961388E-4 52-53 3.2615349892581344E-5 6.523069978516269E-5 3.2615349892581344E-5 0.28655846415621966 5.055379233350108E-4 54-55 3.2615349892581344E-5 6.523069978516269E-5 3.2615349892581344E-5 0.3507454727448198 5.381532732275922E-4 56-57 3.2615349892581344E-5 6.523069978516269E-5 3.2615349892581344E-5 0.42422785605280555 5.707686231201736E-4 58-59 3.2615349892581344E-5 6.523069978516269E-5 3.2615349892581344E-5 0.5076089980531897 5.870762980664643E-4 60-61 3.2615349892581344E-5 6.523069978516269E-5 3.2615349892581344E-5 0.5969261337340237 5.870762980664643E-4 62-63 3.2615349892581344E-5 6.523069978516269E-5 3.2615349892581344E-5 0.6924401858944482 6.523069978516269E-4 64-65 3.2615349892581344E-5 8.153837473145336E-5 3.2615349892581344E-5 0.7903025432471386 6.523069978516269E-4 66-67 3.2615349892581344E-5 9.784604967774404E-5 3.2615349892581344E-5 0.9014393480061095 6.523069978516269E-4 68-69 3.2615349892581344E-5 9.784604967774404E-5 3.2615349892581344E-5 1.0258995231961998 6.523069978516269E-4 70-71 3.2615349892581344E-5 9.784604967774404E-5 3.2615349892581344E-5 1.1622643010970826 6.523069978516269E-4 72-73 3.2615349892581344E-5 9.784604967774404E-5 3.2615349892581344E-5 1.30854414536531 7.175376976367896E-4 74-75 3.2615349892581344E-5 9.784604967774404E-5 3.2615349892581344E-5 1.4574169099499974 7.175376976367896E-4 76-77 3.2615349892581344E-5 9.784604967774404E-5 3.2615349892581344E-5 1.616269971601815 7.175376976367896E-4 78-79 6.523069978516269E-5 9.784604967774404E-5 3.2615349892581344E-5 1.801411005267053 7.175376976367896E-4 80-81 9.784604967774404E-5 1.1415372462403472E-4 3.2615349892581344E-5 2.008942476633548 7.175376976367896E-4 82-83 9.784604967774404E-5 1.3046139957032538E-4 3.2615349892581344E-5 2.225100708046631 7.338453725830802E-4 84-85 9.784604967774404E-5 1.3046139957032538E-4 3.2615349892581344E-5 2.4415361699338005 7.501530475293709E-4 86-87 9.784604967774404E-5 1.3046139957032538E-4 3.2615349892581344E-5 2.6752414595890923 7.664607224756616E-4 88-89 9.784604967774404E-5 1.3046139957032538E-4 3.2615349892581344E-5 2.9275864217079945 8.153837473145336E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2620 0.0 52.6959 1 GTATCAA 5055 0.0 42.961918 1 TCAACGC 6100 0.0 36.07079 4 ATCAACG 6015 0.0 35.92029 3 TATCAAC 6330 0.0 34.2206 2 CAACGCA 6530 0.0 33.985157 5 AACGCAG 7040 0.0 31.634075 6 GTACATG 5960 0.0 28.99849 1 ACGCAGA 7995 0.0 27.754728 7 TACATGG 6270 0.0 26.754665 2 CGCAGAG 8500 0.0 26.45166 8 ACATGGG 6630 0.0 25.447754 3 GCAGAGT 9455 0.0 22.49896 9 CATGGGG 4545 0.0 21.380974 4 TATCACG 380 0.0 21.210907 2 ATCACGC 395 0.0 20.400513 3 AGAGTAC 8540 0.0 20.170507 10-11 GTGGTAT 1370 0.0 19.860983 1 GAGTACA 4290 0.0 19.380018 1 CAGAGTA 9010 0.0 19.069242 10-11 >>END_MODULE