FastQCFastQC Report
Sat 18 Jun 2016
SRR1930930_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930930_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3066041
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60550.19748594359958005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48700.15883675397687116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31910.10407558150722707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33600.053.8339231
GTATCAA67400.042.1222571
TCAACGC83150.033.5062454
CAACGCA84800.033.1292765
ATCAACG84200.032.9778253
TATCAAC88950.031.7504022
AACGCAG89550.031.4774766
ACGCAGA103400.027.1694097
CGCAGAG106450.026.3463758
GTACATG83000.025.7397771
TACATGG84450.024.1683522
ACATGGG82450.024.1213463
GCAGAGT124250.022.381049
TAACGCA3800.021.2331894
AGAGTAC113650.020.71053310-11
CATGGGG54300.020.366014
TATAACG4050.019.9237982
CAGAGTA119100.018.84640110-11
GAGTACT85850.018.2411712-13
AGTACTT93450.018.12875412-13
Produced by FastQC (version 0.11.5)