##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930930_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3066041 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.256463954656837 31.0 31.0 33.0 30.0 34.0 2 31.36651858210637 31.0 31.0 34.0 30.0 34.0 3 31.451436233240194 31.0 31.0 34.0 30.0 34.0 4 35.325468902731565 37.0 35.0 37.0 33.0 37.0 5 35.11718205986156 37.0 35.0 37.0 32.0 37.0 6 35.15880218170599 37.0 35.0 37.0 32.0 37.0 7 35.04158815880153 37.0 35.0 37.0 32.0 37.0 8 35.02077956557006 37.0 35.0 37.0 32.0 37.0 9 36.5260653722504 38.0 35.0 39.0 32.0 39.0 10-11 36.45233299228549 38.0 35.0 39.0 32.0 39.0 12-13 36.352414400198825 38.0 35.0 39.0 32.0 39.0 14-15 37.36723220596202 39.0 36.0 41.0 32.0 41.0 16-17 37.34292741029881 39.0 36.0 41.0 32.0 41.0 18-19 37.33443681933804 39.0 36.0 40.5 32.0 41.0 20-21 37.30240120728979 39.0 36.0 40.0 31.5 41.0 22-23 37.17059523992015 39.0 36.0 40.0 31.0 41.0 24-25 37.05041550324995 39.0 36.0 40.0 31.0 41.0 26-27 36.77635540424932 39.0 35.5 40.0 30.5 41.0 28-29 36.585917637761526 38.0 35.0 40.0 30.0 41.0 30-31 36.33354364798122 38.0 35.0 40.0 30.0 41.0 32-33 36.080574428065376 38.0 35.0 40.0 30.0 41.0 34-35 36.246508934485874 38.0 35.0 40.0 30.0 41.0 36-37 36.28125455595669 38.0 35.0 40.0 30.0 41.0 38-39 36.23437015356285 38.0 35.0 40.0 30.0 41.0 40-41 36.13065741782318 38.0 35.0 40.0 30.0 41.0 42-43 35.98101607251827 38.0 35.0 40.0 29.0 41.0 44-45 35.78562664360979 38.0 35.0 40.0 28.5 41.0 46-47 35.5464860058949 38.0 34.0 40.0 27.5 41.0 48-49 35.33471421288887 38.0 34.0 40.0 27.0 41.0 50-51 35.061264999391724 38.0 34.0 40.0 26.5 41.0 52-53 34.84539769689968 38.0 33.5 40.0 26.0 41.0 54-55 34.67613055402717 38.0 33.0 40.0 26.0 41.0 56-57 34.43281319460503 37.5 33.0 40.0 25.5 41.0 58-59 34.19952489219811 37.0 33.0 40.0 25.0 41.0 60-61 33.898277452910776 37.0 32.5 40.0 24.0 41.0 62-63 33.60353514515951 36.5 32.0 39.5 23.5 41.0 64-65 33.306872608683314 36.0 32.0 39.0 23.0 41.0 66-67 32.97701759369819 36.0 31.0 39.0 22.5 40.0 68-69 32.613148682617094 35.0 31.0 39.0 22.0 40.0 70-71 32.20892414680691 35.0 31.0 38.0 21.5 40.0 72-73 31.792418790224918 35.0 30.5 37.5 20.0 40.0 74-75 31.26705888799269 34.0 30.0 37.0 18.5 39.0 76-77 29.908841075510733 33.5 28.5 35.5 18.0 38.5 78-79 30.6726725115548 34.0 29.5 36.0 18.0 39.0 80-81 30.667441009432032 34.0 30.0 36.0 18.0 37.5 82-83 30.379589346652573 34.0 30.0 35.0 18.0 37.0 84-85 30.04795761048205 34.0 29.5 35.0 16.0 37.0 86-87 29.701783994408423 34.0 29.0 35.0 12.0 36.0 88-89 29.34811586015973 34.0 29.0 35.0 7.5 36.0 90-91 28.996197702509523 34.0 29.0 35.0 4.5 35.5 92-93 28.647787978047262 33.5 29.0 35.0 2.0 35.0 94-95 28.222598132249374 33.0 28.5 35.0 2.0 35.0 96-97 27.759157167174216 33.0 27.0 35.0 2.0 35.0 98-99 27.261334731009796 33.0 27.0 35.0 2.0 35.0 100-101 26.048342960841033 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5335407315041074 1101 2 0.5618053588311938 1101 3 0.5712164002774571 1101 4 0.3936043735988264 1101 5 0.4842192758220065 1101 6 0.5082257670406776 1101 7 0.5076590832046293 1101 8 0.4968789644433045 1101 9 0.6584588570931302 1101 10-11 0.659449743829768 1101 12-13 0.7073022717276842 1101 14-15 0.7489956914898883 1101 16-17 0.7865467859505202 1101 18-19 0.7616472373538912 1101 20-21 0.6580945197111134 1101 22-23 0.7483519064227977 1101 24-25 0.6903393330197218 1101 26-27 0.760657326446001 1101 28-29 0.8644684784662928 1101 30-31 0.8023149891312471 1101 32-33 0.8147651136571739 1101 34-35 0.8215135552177415 1101 36-37 0.8377695309208804 1101 38-39 0.8714095312245504 1101 40-41 0.8591975320561289 1101 42-43 0.8853506004064187 1101 44-45 0.9193659454092611 1101 46-47 0.8372501815100577 1101 48-49 0.9842825810913993 1101 50-51 0.9030588314135244 1101 52-53 0.9760826636846502 1101 54-55 1.0202675872434739 1101 56-57 1.0449277873123606 1101 58-59 0.8552397830390674 1101 60-61 0.9072723163370213 1101 62-63 0.9352514259307085 1101 64-65 0.8860121313259981 1101 66-67 1.1251108456125323 1101 68-69 1.033337801984402 1101 70-71 0.9785809470605713 1101 72-73 0.8227915820831768 1101 74-75 0.8176707412160091 1101 76-77 0.948755255961057 1101 78-79 0.9928575112981051 1101 80-81 0.8688362763167099 1101 82-83 1.033082519444843 1101 84-85 1.1389216188151643 1101 86-87 1.034555211512373 1101 88-89 0.9749608921312465 1101 90-91 0.9309705908857211 1101 92-93 1.1388631787432608 1101 94-95 1.157104147777833 1101 96-97 1.1553431575801696 1101 98-99 1.1527795747859955 1101 100-101 1.4363309604171235 1102 1 0.5124710730521578 1102 2 0.5423182363086987 1102 3 0.5292635938918799 1102 4 0.3325573545404765 1102 5 0.4833767518469969 1102 6 0.4709356178216879 1102 7 0.4382169749904463 1102 8 0.4872836700518377 1102 9 0.6506025643467268 1102 10-11 0.6423443595683764 1102 12-13 0.5902137612199354 1102 14-15 0.6902639206978733 1102 16-17 0.6391908343818926 1102 18-19 0.6751166715277392 1102 20-21 0.7107948492518261 1102 22-23 0.7233463788138508 1102 24-25 0.6504297848025828 1102 26-27 0.7295956023609662 1102 28-29 0.8135966502294636 1102 30-31 0.8463408340090837 1102 32-33 0.8949680218671219 1102 34-35 0.9289777844468929 1102 36-37 1.0566043426912444 1102 38-39 1.0261835340068757 1102 40-41 1.040513208778286 1102 42-43 0.9806929154431074 1102 44-45 1.0493914940707327 1102 46-47 1.074044591484082 1102 48-49 0.9838588858980373 1102 50-51 0.9616148295304399 1102 52-53 1.0372848789598876 1102 54-55 0.9605371970313072 1102 56-57 1.00006491962057 1102 58-59 1.0630375201576285 1102 60-61 1.0553091952744538 1102 62-63 0.848743076331921 1102 64-65 0.8482361872685757 1102 66-67 0.826713587729742 1102 68-69 0.9472847792550851 1102 70-71 0.847311448353878 1102 72-73 0.813658491012518 1102 74-75 0.8844850928988883 1102 76-77 1.0658869294248312 1102 78-79 1.2355758254038207 1102 80-81 1.0371282715984087 1102 82-83 1.188730096024706 1102 84-85 1.2279726685319083 1102 86-87 1.2188735171547656 1102 88-89 1.1677059715013627 1102 90-91 1.084495406940647 1102 92-93 1.2156864698128658 1102 94-95 1.2016995215129604 1102 96-97 1.234724435647994 1102 98-99 1.0887450550003308 1102 100-101 1.316830825532886 1103 1 0.3340600211255449 1103 2 0.4016749942440967 1103 3 0.3431566879952328 1103 4 0.2988611267736019 1103 5 0.2797926631325396 1103 6 0.39401819577390285 1103 7 0.4300728763080741 1103 8 0.33904986012753824 1103 9 0.4741773081401419 1103 10-11 0.3953228973997369 1103 12-13 0.38794216666491366 1103 14-15 0.504962702743164 1103 16-17 0.5491796204766501 1103 18-19 0.4451637076302788 1103 20-21 0.45155038611753895 1103 22-23 0.3930722611301434 1103 24-25 0.5160520007948506 1103 26-27 0.5710473358438151 1103 28-29 0.495378259424875 1103 30-31 0.568441883755213 1103 32-33 0.5892506434289757 1103 34-35 0.6164513850449609 1103 36-37 0.6582320721869337 1103 38-39 0.6850887765075768 1103 40-41 0.6992840401647626 1103 42-43 0.7396934761383633 1103 44-45 0.919064733331858 1103 46-47 0.8408526779557519 1103 48-49 0.7848681373698625 1103 50-51 0.7925742414234094 1103 52-53 0.6923710079821035 1103 54-55 0.8191452710430838 1103 56-57 0.7873312187203396 1103 58-59 0.8466913842824724 1103 60-61 0.8004323924834367 1103 62-63 0.7777596791416315 1103 64-65 0.6980045889755786 1103 66-67 0.5319911318844959 1103 68-69 0.7312763065266736 1103 70-71 0.6668264469521361 1103 72-73 0.6066719165490753 1103 74-75 0.6745799438474016 1103 76-77 0.8893471979355603 1103 78-79 1.0450003340167235 1103 80-81 0.9623475657453717 1103 82-83 0.8993805025287713 1103 84-85 0.8870872372638026 1103 86-87 0.8246796723427536 1103 88-89 0.7629828926613804 1103 90-91 0.8127237656610191 1103 92-93 0.902730982786732 1103 94-95 0.8346536068009378 1103 96-97 0.7195675967232802 1103 98-99 0.8585405569783369 1103 100-101 1.042381708627925 1104 1 0.3528435298224082 1104 2 0.47139091180702763 1104 3 0.46386214533424663 1104 4 0.2105055407482297 1104 5 0.3912372404772597 1104 6 0.3127808780255563 1104 7 0.2955113499646487 1104 8 0.3841152081641752 1104 9 0.46355663145084947 1104 10-11 0.4563322263506038 1104 12-13 0.5019789071074356 1104 14-15 0.5115860390586491 1104 16-17 0.5266086565931118 1104 18-19 0.6297131594976904 1104 20-21 0.5694758776864717 1104 22-23 0.5087974022961674 1104 24-25 0.5541384637675435 1104 26-27 0.5961095720691887 1104 28-29 0.5930192390619524 1104 30-31 0.44336793771920924 1104 32-33 0.6060619201792079 1104 34-35 0.5216389932290255 1104 36-37 0.5279756232822308 1104 38-39 0.5517853457356452 1104 40-41 0.6856775643558564 1104 42-43 0.6567193382073313 1104 44-45 0.676862089588866 1104 46-47 0.6737473998700381 1104 48-49 0.7012485417108465 1104 50-51 0.6984763911663592 1104 52-53 0.6922474269356229 1104 54-55 0.7750791061559781 1104 56-57 0.6904357771997098 1104 58-59 0.7595187322413182 1104 60-61 0.7739110895421248 1104 62-63 0.6306741254012991 1104 64-65 0.6780298400274347 1104 66-67 0.7354908255655701 1104 68-69 0.7700708281181079 1104 70-71 0.7141490737098266 1104 72-73 0.5637268951229544 1104 74-75 0.8206600340899293 1104 76-77 0.9152945580823335 1104 78-79 1.0575744016078588 1104 80-81 0.8178638995130605 1104 82-83 1.0148627360313114 1104 84-85 1.081305589170757 1104 86-87 0.8552232344590429 1104 88-89 0.9463781785914556 1104 90-91 0.8370388430349109 1104 92-93 0.8105212888463385 1104 94-95 0.818335236093457 1104 96-97 0.8484267694352887 1104 98-99 1.0672213353849607 1104 100-101 1.3317601000159875 1105 1 0.18706064403324518 1105 2 0.29149596478266204 1105 3 0.3344212104478643 1105 4 0.208326885287633 1105 5 0.2064043426320623 1105 6 0.23499394440308663 1105 7 0.21192205572333478 1105 8 0.30615777620985085 1105 9 0.3286728500480365 1105 10-11 0.27647510217975224 1105 12-13 0.2897505914221412 1105 14-15 0.42185235331613313 1105 16-17 0.3329379663890748 1105 18-19 0.4459132983390859 1105 20-21 0.3426230700487807 1105 22-23 0.39904311491812905 1105 24-25 0.49440219149342113 1105 26-27 0.47264868641556035 1105 28-29 0.5877413257960669 1105 30-31 0.5979630893809755 1105 32-33 0.5798131060832574 1105 34-35 0.5376011731334316 1105 36-37 0.5156042997817849 1105 38-39 0.531520865492574 1105 40-41 0.5449220804360806 1105 42-43 0.4951332624544591 1105 44-45 0.553888961150335 1105 46-47 0.6714610379242387 1105 48-49 0.6020966699523811 1105 50-51 0.6928058195527242 1105 52-53 0.7332796086681341 1105 54-55 0.8392276506853023 1105 56-57 0.7695046987125593 1105 58-59 0.7378141763998158 1105 60-61 0.6254872190664926 1105 62-63 0.5524706971536162 1105 64-65 0.7004576101968709 1105 66-67 0.8425835266216168 1105 68-69 0.7514012607800282 1105 70-71 0.7250587015171917 1105 72-73 0.7504893494794374 1105 74-75 0.83668884722454 1105 76-77 0.7405729204946141 1105 78-79 0.8049487666961035 1105 80-81 0.7856910317370271 1105 82-83 0.9207248055402601 1105 84-85 0.8344511898213121 1105 86-87 0.8998088929785659 1105 88-89 0.9459619794572838 1105 90-91 0.8220152904288369 1105 92-93 0.9361460554084147 1105 94-95 0.8195455674058927 1105 96-97 0.7003050385511962 1105 98-99 0.8285933574262287 1105 100-101 1.1315968806134897 1106 1 0.08803329944365146 1106 2 0.06321129447005092 1106 3 0.10541368770050852 1106 4 -0.026902307748450482 1106 5 0.09715036035230895 1106 6 0.0779399955577702 1106 7 0.12117632458394212 1106 8 0.20704101839455546 1106 9 0.15389712344616413 1106 10-11 0.058239293902339284 1106 12-13 0.09151707579154333 1106 14-15 0.0980312752944954 1106 16-17 5.341739691360203E-4 1106 18-19 0.03554193731885391 1106 20-21 0.05360785738190543 1106 22-23 0.1706622012028447 1106 24-25 -0.05459835778654565 1106 26-27 -0.0019903888835628436 1106 28-29 -0.04240334977053095 1106 30-31 0.021172603865245776 1106 32-33 0.09209247465011572 1106 34-35 0.11494151177647183 1106 36-37 0.0955849555742958 1106 38-39 0.12977577916009153 1106 40-41 0.12828806806534487 1106 42-43 0.133430876668875 1106 44-45 0.017620387128893356 1106 46-47 0.08067623626206455 1106 48-49 0.10618909227702744 1106 50-51 -0.055554651395141263 1106 52-53 -0.2672592110963947 1106 54-55 -0.06237462285611883 1106 56-57 0.015609635067725947 1106 58-59 0.10912757644280191 1106 60-61 -0.06809418183624416 1106 62-63 -0.12053897495012933 1106 64-65 -0.09301584525174889 1106 66-67 -0.23199560551604037 1106 68-69 -0.023399398355927303 1106 70-71 -0.10420449911328689 1106 72-73 -0.11207884482238128 1106 74-75 0.011956012223468093 1106 76-77 0.12465115706710961 1106 78-79 0.18444261714866172 1106 80-81 -0.047739770928171055 1106 82-83 0.0710728102210787 1106 84-85 0.06751488241558334 1106 86-87 -0.070193399668252 1106 88-89 0.0010030321642773288 1106 90-91 -0.13498927098305558 1106 92-93 -0.05659370529657792 1106 94-95 0.012801460224654448 1106 96-97 0.07461936998194929 1106 98-99 0.06232354155442721 1106 100-101 0.19618542215577506 1107 1 0.0879951916287176 1107 2 0.16772027904770326 1107 3 0.19294620668367202 1107 4 0.11975957972030216 1107 5 0.1678296038555871 1107 6 0.22084524481495293 1107 7 0.14546326511122487 1107 8 0.0029980056133425137 1107 9 0.07883812236141097 1107 10-11 0.14178492423766897 1107 12-13 0.12380505390784435 1107 14-15 0.08181706032491576 1107 16-17 0.13618639299474466 1107 18-19 0.16344978054858217 1107 20-21 0.22233747111580726 1107 22-23 0.2630068694101766 1107 24-25 0.2140334396006267 1107 26-27 0.05854736520783632 1107 28-29 0.18298352944467666 1107 30-31 0.15864267885463335 1107 32-33 0.18349633259474984 1107 34-35 0.06049912154867343 1107 36-37 0.2943620154243263 1107 38-39 0.2721915693784567 1107 40-41 0.17007819210439834 1107 42-43 0.27449600896147075 1107 44-45 0.19182752762787914 1107 46-47 0.2559652048042551 1107 48-49 0.14739165795169384 1107 50-51 0.1658493008757631 1107 52-53 0.0605402512438431 1107 54-55 0.2308320931746337 1107 56-57 0.15997260257232426 1107 58-59 0.18199386894858094 1107 60-61 0.09492397597161784 1107 62-63 -0.008181101064359098 1107 64-65 0.028938783810176005 1107 66-67 0.011544082263995392 1107 68-69 0.20493423367727814 1107 70-71 0.18768664836584037 1107 72-73 0.05781918847479517 1107 74-75 0.36499652535840355 1107 76-77 0.33419990016949797 1107 78-79 0.3179517432157297 1107 80-81 0.40120981167806136 1107 82-83 0.49487406476053053 1107 84-85 0.5427879098173669 1107 86-87 0.41420343096892154 1107 88-89 0.5114202372767593 1107 90-91 0.44865794875000375 1107 92-93 0.45739308902498266 1107 94-95 0.41433454516546675 1107 96-97 0.5256119653693965 1107 98-99 0.6253865986689817 1107 100-101 0.89079816563547 1108 1 0.24388409257376154 1108 2 0.3303443575689968 1108 3 0.31230082188828234 1108 4 0.05994827450511764 1108 5 0.1392698101988401 1108 6 0.20653626069987752 1108 7 0.248534228939711 1108 8 0.29472976461634914 1108 9 0.2804378577651505 1108 10-11 0.28244614036078275 1108 12-13 0.2381099200475063 1108 14-15 0.3845604816957717 1108 16-17 0.4478364069174319 1108 18-19 0.4340409649436836 1108 20-21 0.38431574650770983 1108 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66-67 -0.8707329048869994 2316 68-69 -1.003982851573042 2316 70-71 -0.8232757241845157 2316 72-73 -0.9598499655241959 2316 74-75 -0.6181868328302649 2316 76-77 -0.876115986313895 2316 78-79 -0.9378388981823562 2316 80-81 -0.9981480527819961 2316 82-83 -1.0402051227004208 2316 84-85 -1.058986824165327 2316 86-87 -0.7623791772203639 2316 88-89 -0.9547487943004214 2316 90-91 -0.7627649613726035 2316 92-93 -0.9582663238404834 2316 94-95 -0.700020406577412 2316 96-97 -0.6265813859910843 2316 98-99 -0.5459980290641724 2316 100-101 -0.653646514884084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 48.0 5 127.0 6 226.0 7 605.0 8 1553.0 9 2722.0 10 4461.0 11 6944.0 12 9756.0 13 12444.0 14 13867.0 15 16693.0 16 19471.0 17 21930.0 18 19601.0 19 16700.0 20 16650.0 21 18397.0 22 19863.0 23 23098.0 24 28882.0 25 35685.0 26 45130.0 27 57791.0 28 73991.0 29 91328.0 30 112260.0 31 135808.0 32 162673.0 33 196555.0 34 242011.0 35 300499.0 36 384416.0 37 487254.0 38 415347.0 39 71225.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.83708130357923 21.111410868988663 14.20639210657404 23.84511572085807 2 15.307976598299177 22.18706161358491 39.897929862087565 22.60703192602834 3 18.054679898876465 27.23466406693123 31.247674051102543 23.462981983089758 4 12.611622363558892 18.22453476679776 39.27224665924094 29.891596210402405 5 12.334646010858455 38.53270615310252 36.02680106118791 13.105846774851127 6 29.446028101394635 39.21361104225646 18.28188803799037 13.058472818358535 7 25.328885034479313 33.354511567196916 24.05551654397316 17.26108685435061 8 24.5608913905587 36.561807229583685 21.403268906058333 17.474032473799276 9 25.48080733427896 16.099621629325895 21.407313209445014 37.012257826950126 10-11 23.740647956110177 26.861676018031073 29.873361119437085 19.52431490642167 12-13 24.42097153951953 24.540751412000038 29.80348273229223 21.234794316188204 14-15 21.59206638007554 26.10381369717719 27.28905570555901 25.01506421718826 16-17 21.35953172185238 28.969834389037853 27.98785143447201 21.682782454637756 18-19 21.26362628549325 27.96244407690569 29.830162088504363 20.9437675490967 20-21 21.925717888312647 27.270085429385976 29.75653293612186 21.04766374617952 22-23 21.586909633628512 27.208328264364372 29.645069977863965 21.559692124143155 24-25 21.493472744953284 27.35406732194433 29.641260673212923 21.511199259889462 26-27 21.46312459036337 27.458582159469756 29.488863183853642 21.589430066313227 28-29 21.495727631689025 27.651225628371716 29.420902763972872 21.43214397596639 30-31 21.740455699670726 27.37569047257925 29.428181881934286 21.45567194581574 32-33 21.562234433847625 27.54308186410516 29.310788041306395 21.583895660740822 34-35 21.81314833293157 27.56867953984325 29.26327882723767 21.354893299987506 36-37 21.87803528876869 27.402676696614304 29.23179185510326 21.487496159513743 38-39 21.69025454029535 27.51725976776062 29.2156029768258 21.576882715118227 40-41 21.84872880651094 27.460284274800493 29.217831705847765 21.4731552128408 42-43 21.776217184179583 27.536356920005804 28.97898906805272 21.708436827761897 44-45 21.71336784215078 27.663162454397916 29.015865912384964 21.60760379106634 46-47 21.890788084419047 27.713866620827638 28.611821184740954 21.783524110012358 48-49 21.845344707179034 27.78329392477592 28.493675440276057 21.87768592776899 50-51 21.895955801018314 27.893467043073876 28.41451805967072 21.796059096237094 52-53 22.05717901252092 27.763365661070743 28.358607835209792 21.82084749119855 54-55 21.79822548345121 27.771536295253874 28.554064885103987 21.87617333619093 56-57 21.875859810396875 27.944321063946 28.286198394862932 21.89362073079419 58-59 21.840652305463987 27.89667698059166 28.40564226610579 21.857028447838562 60-61 21.84444047775607 27.994419152273736 28.43221972001779 21.728920649952403 62-63 21.855525750959117 27.92969996567203 28.40399079429186 21.810783489076996 64-65 21.62608516654606 27.774892942313972 28.435987314849747 22.163034576290222 66-67 21.617652028309305 27.943690262159627 28.30957981552558 22.129077894005487 68-69 21.77228133795362 27.870382417283913 28.356576500189444 22.000759744573024 70-71 21.693055970875243 27.639300399969187 28.35579759443935 22.31184603471623 72-73 21.692542760054618 27.83436362826352 28.204215600984163 22.268878010697705 74-75 21.68745584119078 27.978563789596617 28.11655342721402 22.217426941998582 76-77 21.636411782206704 27.80919656676424 28.164463226114368 22.389928424914693 78-79 21.553717331553713 27.937781113479698 28.133193664406097 22.375307890560492 80-81 21.619618843569242 27.960560487134504 27.9965844876968 22.42323618159945 82-83 21.69967720588211 27.890706614816956 28.046836294752747 22.362779884548186 84-85 21.66390169992862 27.893809621888554 27.92088036671401 22.521408311468814 86-87 21.509448177633633 27.962998537853867 27.978001598804454 22.549551685708053 88-89 21.500390569579277 27.832819912362382 27.9400920080821 22.72669750997624 90-91 21.45734349627207 27.813077991046843 28.016708604526876 22.71286990815421 92-93 21.506210812883573 27.699245196665274 28.047850587530487 22.746693402920666 94-95 21.29599323935687 27.625876942139715 28.114366676500392 22.963763142003025 96-97 21.304770549382738 27.45387292603067 28.168915549400676 23.072440975185916 98-99 21.127457199691722 27.54778882604636 28.029468620934946 23.295285353326978 100-101 21.10108768930357 27.46639722038942 28.01061694869703 23.42189814160998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 480.0 1 337.0 2 376.5 3 538.0 4 423.0 5 360.0 6 495.5 7 850.5 8 1269.5 9 1488.0 10 1387.5 11 1346.5 12 1526.5 13 1745.5 14 1989.0 15 2185.0 16 2549.0 17 2930.5 18 3250.5 19 3735.5 20 4343.0 21 5136.5 22 6292.5 23 8023.5 24 10464.5 25 13957.5 26 18791.5 27 24726.5 28 32139.0 29 40930.5 30 50400.0 31 60537.5 32 70767.5 33 77058.5 34 80406.0 35 83632.5 36 89285.0 37 95904.0 38 99054.0 39 100570.0 40 101501.5 41 102773.0 42 105166.5 43 107420.5 44 108666.0 45 110570.0 46 112001.0 47 111813.5 48 111003.0 49 108800.0 50 104919.0 51 100368.5 52 96705.5 53 93825.0 54 89627.5 55 82008.5 56 72465.0 57 62882.0 58 54903.0 59 47726.5 60 39345.5 61 32079.5 62 26516.0 63 20471.0 64 15067.0 65 11311.0 66 8183.5 67 6365.0 68 4992.0 69 3721.5 70 2877.0 71 2193.5 72 1672.0 73 1334.5 74 997.0 75 757.5 76 574.5 77 351.5 78 219.0 79 152.0 80 102.0 81 62.0 82 42.0 83 24.5 84 19.0 85 13.0 86 5.0 87 2.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019699671335119132 2 0.003587688488183948 3 0.0028701507905471587 4 0.03323504154054039 5 0.001402460045380998 6 3.2615349892581344E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6307674946290672E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.076918736572668E-4 26-27 0.002005844018393753 28-29 4.892302483887202E-5 30-31 0.0067187620778717564 32-33 0.021395669529533364 34-35 0.012719986458106724 36-37 7.501530475293709E-4 38-39 7.664607224756617E-4 40-41 0.002087382393125206 42-43 0.008349529572500824 44-45 0.008300606547661953 46-47 0.007827683974219523 48-49 0.009588912868418915 50-51 0.1917782573683783 52-53 0.0485968713399462 54-55 0.017286135443068115 56-57 0.010485834990464902 58-59 0.019764902034904296 60-61 0.05322825102469275 62-63 0.09593805170902803 64-65 0.10076512349313006 66-67 0.10539650317787663 68-69 0.10337435148453657 70-71 0.09148605644869068 72-73 0.06889992664807809 74-75 0.24389758649672333 76-77 0.29489168605377425 78-79 0.007159069301421605 80-81 9.621528218311497E-4 82-83 0.0 84-85 1.6307674946290672E-5 86-87 0.0 88-89 1.9569209935548808E-4 90-91 0.006376300903999653 92-93 0.017628596616940218 94-95 0.0019732286685011716 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3066041.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.381437096716105 #Duplication Level Percentage of deduplicated Percentage of total 1 80.53728346588373 50.2403148246742 2 11.481760991082183 14.32497502049444 3 3.351513979140832 6.272167754056156 4 1.4358276327126789 3.5827596460717115 5 0.7718620615413783 2.407493231969255 6 0.5066721562145535 1.8964162344933415 7 0.3484336351514347 1.5215053625505548 8 0.25615391268145893 1.2783399352812912 9 0.1942835071956263 1.0907715944748015 >10 1.0411122934785633 11.596190438690524 >50 0.05305384494229805 2.2464273209180043 >100 0.020157859638696055 2.223266431857189 >500 0.0013611435766642036 0.6004123263980383 >1k 4.711650838863617E-4 0.521217336089235 >5k 5.235167598737352E-5 0.19774254198122293 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6055 0.19748594359958005 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4870 0.15883675397687116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3191 0.10407558150722707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2615349892581344E-5 2 0.0 0.0 0.0 0.0 3.2615349892581344E-5 3 0.0 0.0 0.0 0.0 3.2615349892581344E-5 4 0.0 0.0 0.0 0.0 3.2615349892581344E-5 5 0.0 0.0 0.0 0.0 3.2615349892581344E-5 6 0.0 0.0 0.0 0.0 6.523069978516269E-5 7 0.0 0.0 0.0 0.0 6.523069978516269E-5 8 0.0 0.0 0.0 0.0 6.523069978516269E-5 9 0.0 0.0 0.0 0.0 6.523069978516269E-5 10-11 0.0 0.0 0.0 0.0 6.523069978516269E-5 12-13 0.0 0.0 0.0 0.0 9.784604967774403E-5 14-15 0.0 0.0 0.0 0.0 2.1199977430177874E-4 16-17 0.0 0.0 0.0 0.0 2.772304740869414E-4 18-19 0.0 0.0 0.0 0.0 3.587688488183948E-4 20-21 0.0 0.0 0.0 3.2615349892581344E-5 4.4030722354984815E-4 22-23 0.0 0.0 0.0 1.4676907451661607E-4 4.566148984961388E-4 24-25 1.6307674946290672E-5 0.0 0.0 2.9353814903323213E-4 4.566148984961388E-4 26-27 3.2615349892581344E-5 0.0 0.0 6.033839730127549E-4 5.055379233350108E-4 28-29 3.2615349892581344E-5 0.0 0.0 0.0018427672689308458 5.544609481738828E-4 30-31 4.892302483887201E-5 0.0 0.0 0.0064904546286236875 5.707686231201736E-4 32-33 6.523069978516269E-5 0.0 0.0 0.01839505733941588 6.523069978516269E-4 34-35 6.523069978516269E-5 0.0 0.0 0.03509411648441753 7.175376976367896E-4 36-37 6.523069978516269E-5 0.0 0.0 0.06130055012310664 7.175376976367896E-4 38-39 6.523069978516269E-5 0.0 0.0 0.10381465870808643 7.175376976367896E-4 40-41 6.523069978516269E-5 0.0 0.0 0.15079707022834984 7.175376976367896E-4 42-43 6.523069978516269E-5 0.0 0.0 0.1971924054505468 7.338453725830802E-4 44-45 6.523069978516269E-5 0.0 0.0 0.25343757634030334 7.664607224756616E-4 46-47 6.523069978516269E-5 0.0 0.0 0.32145688854128174 7.827683974219523E-4 48-49 6.523069978516269E-5 0.0 0.0 0.39454788765055654 8.153837473145336E-4 50-51 6.523069978516269E-5 0.0 0.0 0.4729388811173758 8.47999097207115E-4 52-53 8.153837473145336E-5 0.0 0.0 0.5518680278574226 8.969221220459869E-4 54-55 9.784604967774404E-5 0.0 0.0 0.6408753177142772 9.132297969922776E-4 56-57 9.784604967774404E-5 0.0 0.0 0.7435973622009621 9.295374719385683E-4 58-59 9.784604967774404E-5 0.0 0.0 0.8594633926943573 9.45845146884859E-4 60-61 9.784604967774404E-5 0.0 0.0 0.9832223378617572 9.784604967774404E-4 62-63 9.784604967774404E-5 0.0 0.0 1.115444966326282 0.0010110758466700218 64-65 9.784604967774404E-5 0.0 0.0 1.25200543632652 0.0010110758466700218 66-67 1.3046139957032538E-4 0.0 0.0 1.4083145006867162 0.0010599988715088936 68-69 1.3046139957032538E-4 0.0 0.0 1.583964467533213 0.0011089218963477657 70-71 1.3046139957032538E-4 0.0 0.0 1.7788737984912792 0.0011578449211866377 72-73 1.6307674946290673E-4 0.0 0.0 1.9884274215511142 0.0011741525961329285 74-75 1.6307674946290673E-4 0.0 0.0 2.2062979588335576 0.0011741525961329285 76-77 1.6307674946290673E-4 0.0 0.0 2.4381604812199185 0.0011741525961329285 78-79 1.6307674946290673E-4 0.0 0.0 2.7078405018067273 0.0012067679460255097 80-81 1.6307674946290673E-4 0.0 0.0 3.006629722172665 0.0012719986458106726 82-83 1.6307674946290673E-4 0.0 0.0 3.3230964621803816 0.001402460045380998 84-85 1.6307674946290673E-4 0.0 0.0 3.6487607308578065 0.001402460045380998 86-87 1.6307674946290673E-4 0.0 0.0 3.9995714343024114 0.00145138307021987 88-89 1.6307674946290673E-4 0.0 0.0 4.390303326015536 0.0015003060950587418 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3360 0.0 53.833923 1 GTATCAA 6740 0.0 42.122257 1 TCAACGC 8315 0.0 33.506245 4 CAACGCA 8480 0.0 33.129276 5 ATCAACG 8420 0.0 32.977825 3 TATCAAC 8895 0.0 31.750402 2 AACGCAG 8955 0.0 31.477476 6 ACGCAGA 10340 0.0 27.169409 7 CGCAGAG 10645 0.0 26.346375 8 GTACATG 8300 0.0 25.739777 1 TACATGG 8445 0.0 24.168352 2 ACATGGG 8245 0.0 24.121346 3 GCAGAGT 12425 0.0 22.38104 9 TAACGCA 380 0.0 21.233189 4 AGAGTAC 11365 0.0 20.710533 10-11 CATGGGG 5430 0.0 20.36601 4 TATAACG 405 0.0 19.923798 2 CAGAGTA 11910 0.0 18.846401 10-11 GAGTACT 8585 0.0 18.24117 12-13 AGTACTT 9345 0.0 18.128754 12-13 >>END_MODULE