FastQCFastQC Report
Sat 18 Jun 2016
SRR1930929_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930929_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753060
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32040.18276613464456437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12950.050.56151
GTATCAA24400.047.8899651
TCAACGC33550.034.1997574
ATCAACG32950.034.0718963
CAACGCA35150.032.321015
TATCAAC35000.032.0823972
AACGCAG38150.029.8926126
GTACATG40000.028.7092081
TACATGG40100.027.3742452
ACATGGG41650.025.383483
ACGCAGA45700.024.6723237
CGCAGAG46700.024.6715748
CATGGGG29950.023.0199554
GCAGAGT50900.021.4524279
GTGGTAT7650.019.7517781
AGAGTAC47600.019.47108510-11
AGTACTT32700.018.65021512-13
GGGTACG1659.881776E-418.3152851
GTATAAG5500.018.3152851
CAGAGTA49400.017.560749
Produced by FastQC (version 0.11.5)